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What is DNA? - mrgscience.com
What is DNA? - mrgscience.com

... Miescher in Germany around 1869. Although the chemical composition of DNA was known in the 1920s, its structure was not determined until the 1950s. James D. Watson and Francis H. C. Crick worked out the structure of DNA in 1953, after long months of research. Watson, Crick and Maurice Wilkins shared ...
Spontaneous Deamin - Oregon State University
Spontaneous Deamin - Oregon State University

... genomic stability (mismatch repair). Protects DNA from restriction endonucleases. ...
Laboratory in Fundamentals of Molecular Biology
Laboratory in Fundamentals of Molecular Biology

... The process of extracting DNA from a cell is the first step for many laboratory procedures in molecular biology. The scientist must be able to separate DNA from the unwanted substances of the cell gently enough so that the DNA is not sheared into smaller pieces or degraded. Such shearing – breaking ...
DNA
DNA

... of all the DNA. – Semi-conservative o One of the original strand, one new ...
Recall that the nucleus is a small spherical, dense body in a cell
Recall that the nucleus is a small spherical, dense body in a cell

... Recall that the nucleus is a small spherical, dense body in a cell. It is often called the "control center" because it controls all the activities of the cell including cell reproduction, and heredity. Chromosomes are microscopic, threadlike strands composed of the chemical DNA (short for deoxyribon ...
What is DNA?
What is DNA?

... set of rules by which information in genetic material is translated into proteins by living cells? ...
DNA structure and replication notes
DNA structure and replication notes

...  Single-strand binding protein (SSB) binds to each strand to prevent reannealing ...
PP4 (Ch.12-25)DNA
PP4 (Ch.12-25)DNA

... •DNA Polymerase can only add to the 3’ end If DNA were synthesized in the 3' to 5' direction, the energy for the process would come from the 5' end of the growing strand rather than from free nucleotides. If the 5' nucleotide needed to be removed this triphosphate end would be lost, losing the energ ...
DNA
DNA

... 1. Think about it! The DNA strand can be incredibly LONG! Human DNA molecules contain up to 4,639,221,000 base pairs. That means there is about 1-2 meters of DNA in each cell. How can it be kept in such a small ...
Visualizing structural variations of single DNA molecules
Visualizing structural variations of single DNA molecules

... Single DNA molecules can be studied using nanofluidics. Individual DNA molecules of genomic length can be stretched by confinement in nanochannels. This has been used to characterize the base pair sequence [1-3], or the methylation [4] of DNA by imaging fluorescence barcodes of single molecules stre ...
DNA, RNA review ap biology summer homework
DNA, RNA review ap biology summer homework

... Color the nucleotides using the same colors as you colored them in the double helix. The two sides of the DNA ladder are held together loosely by hydrogen bonds. The DNA can actually "unzip" when it needs to replicate - or make a copy of itself. DNA needs to copy itself when a cell divides, so that ...
Recall that the nucleus is a small spherical, dense body in a cell
Recall that the nucleus is a small spherical, dense body in a cell

... Recall that the nucleus is a small spherical, dense body in a cell. It is often called the "control center" because it controls all the activities of the cell including cell reproduction, and heredity. Chromosomes are microscopic, threadlike strands composed of the chemical DNA (short for deoxyribon ...
DNA Structure and Protein Synthesis notes-2008
DNA Structure and Protein Synthesis notes-2008

... Structure of DNA is related to 2 primary functions: 1. Copy itself exactly for new cells that are created 2. Store and use information to direct cell activities ...
DNA - Hermantown
DNA - Hermantown

... • DNA is capable of storing, copying and transmitting the genetic information of a cell. ...
Structure of Nucleic Acids
Structure of Nucleic Acids

... DNA is one of the macromolecules, they are essential to all living forms .Deoxyribonucleic acid contains the genetic information, it is used in the development and functioning of all living organisms. The DNA segments carry genetic information are called the genes. Other DNA segments have structural ...
replication of dna
replication of dna

... α-phoshate of the first entering nucleotide with release of pyrophosphate • Elongation-3’-OH of the newly attached nt is then free to carry out nucleophilic attack on the next entering nt ...
NUCLEIC ACIDS
NUCLEIC ACIDS

... like Mg bind to and stabilize the charge on the polymer. Instead, DNA exists usually as a double-stranded (ds) structure with the sugar-phosphate backbones of the two different strands running in opposite directions (5'-3' and the other 3'-5'). The strands are held together by hydrogen bonds between ...
DNApowerpoint
DNApowerpoint

...  DNA and RNA are structurally the same  A mutation in your DNA can turn you into a zombie  You eat DNA everyday  DNA lab techniques are used to solve crime  The first cloned mammal was a cat. ...
DNA and RNA - CPC Kilcullen
DNA and RNA - CPC Kilcullen

... 3. The complementary sands move apart. 4. DNA nucleotides move from the cytoplasm into the nucleus and attach to their complementary bases on the exposed strands. 5. The new strands contain exactly the same sequence of bases. Each new stretch of DNA re-forms into a double helix. ...
12 1 DNA
12 1 DNA

... The Shape of DNA is a Double Helix  DNA often is compared to a twisted ladder.  Rails of the ladder are represented by the alternating deoxyribose and phosphate.  The pairs of bases (cytosine–guanine or thymine–adenine) form the steps. ...
CHAPTER 14: DNA: THE GENETIC MATERIAL
CHAPTER 14: DNA: THE GENETIC MATERIAL

... determining that it was a semiconservative process; each strand served as a template for the production of a new one and each old and new strand then intertwined to become a new helix. Double-stranded DNA replication is complicated since new nucleotides must be added to both the 5’ to 3’ strand and ...
DNA - TeacherWeb
DNA - TeacherWeb

... perhaps the most significant tool to arise from DNA typing is the ability to compare DNA types recovered from crime scene evidence to those of convicted criminals CODIS (COmbined Dna Index System) is a ...
BIO 10 Lecture 1
BIO 10 Lecture 1

... • Enzymes called DNA polymerases catalyze the elongation of new DNA at a replication fork • Most DNA polymerases require a primer and a DNA template strand • The rate of elongation is about 500 nucleotides per second in bacteria and 50 per second in human cells ...
Dephosphorylation of Plasmid DNA Buffers and Solutions EDTA (0.5
Dephosphorylation of Plasmid DNA Buffers and Solutions EDTA (0.5

... electrophoresis through a 0.7% agarose gel containing ethidium bromide, using undigested plasmid DNA as a marker. If digestion is not complete, add more restriction enzyme and continue the incubation. 3. When digestion is complete, extract the sample once with phenol:chloroform and recover the DNA b ...
DNA Replication and Protein Synthesis Virtual Lab
DNA Replication and Protein Synthesis Virtual Lab

... http://tinyurl.com/7qt6wwu 1. Most organisms have the same sort of very molecule ...
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DNA replication



DNA replication is the process of producing two identical replicas from one original DNA molecule. This biological process occurs in all living organisms and is the basis for biological inheritance. DNA is made up of two strands and each strand of the original DNA molecule serves as a template for the production of the complementary strand, a process referred to as semiconservative replication. Cellular proofreading and error-checking mechanisms ensure near perfect fidelity for DNA replication.In a cell, DNA replication begins at specific locations, or origins of replication, in the genome. Unwinding of DNA at the origin and synthesis of new strands results in replication forks growing bidirectional from the origin. A number of proteins are associated with the replication fork which helps in terms of the initiation and continuation of DNA synthesis. Most prominently, DNA polymerase synthesizes the new DNA by adding complementary nucleotides to the template strand.DNA replication can also be performed in vitro (artificially, outside a cell). DNA polymerases isolated from cells and artificial DNA primers can be used to initiate DNA synthesis at known sequences in a template DNA molecule. The polymerase chain reaction (PCR), a common laboratory technique, cyclically applies such artificial synthesis to amplify a specific target DNA fragment from a pool of DNA.
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