Chapter 22: SV40 and Polyomavirus DNA Replication
... stimulating the synthesis of cellular enzymes whose activity is needed for viral DNA replication. For example, large T antigen binds to ~ 1 0 5 ~ ~ / E 2 F complexes, resulting in the release and activation of the transcription activator E2F, whose target genes encode enzymes such as thymidine kinas ...
... stimulating the synthesis of cellular enzymes whose activity is needed for viral DNA replication. For example, large T antigen binds to ~ 1 0 5 ~ ~ / E 2 F complexes, resulting in the release and activation of the transcription activator E2F, whose target genes encode enzymes such as thymidine kinas ...
Specific inhibition of DNA polymerase (3 by its 14 kDa domain: role
... DNA polymerases play a critical role in each DNArepairpathway. Mammalian cells contain five known DNA polymerases: a, P, y, 8, e (8). Two mammalian DNArepairsystems have been shown to require P-polymerase for filling short gaps in vitro (9,10) and it has been suggested that p-polymerase is involved ...
... DNA polymerases play a critical role in each DNArepairpathway. Mammalian cells contain five known DNA polymerases: a, P, y, 8, e (8). Two mammalian DNArepairsystems have been shown to require P-polymerase for filling short gaps in vitro (9,10) and it has been suggested that p-polymerase is involved ...
molecular biology
... of the purine rings and C4, C5 and C6 of the pyrimidine rings line up in the major groove. Most of these groups distinguish one base from another. The minor groove is paved with C2 and N3 of the purine and C2 of the pyrimidine rings. These two grooves of different width appear between ribose-phospha ...
... of the purine rings and C4, C5 and C6 of the pyrimidine rings line up in the major groove. Most of these groups distinguish one base from another. The minor groove is paved with C2 and N3 of the purine and C2 of the pyrimidine rings. These two grooves of different width appear between ribose-phospha ...
DNA Profile 911 - Bertino Forensics
... other activities that provide additional background about DNA profiling and also provided for more advanced students who want to go further. Students use a photographed copy of an electropherogram given to the family of the 911 victim. The victim's wife and her two sons wanted to confirm that their ...
... other activities that provide additional background about DNA profiling and also provided for more advanced students who want to go further. Students use a photographed copy of an electropherogram given to the family of the 911 victim. The victim's wife and her two sons wanted to confirm that their ...
University of Groningen DNA Block Copolymers Schnitzler
... increasingly complex. One strategy for altering polymer properties is to combine different polymer segments within one polymer, known as block copolymers. The microphase separation of the individual polymer components and the resulting formation of well defined nanosized domains provide a broad rang ...
... increasingly complex. One strategy for altering polymer properties is to combine different polymer segments within one polymer, known as block copolymers. The microphase separation of the individual polymer components and the resulting formation of well defined nanosized domains provide a broad rang ...
An attempt to unify the structure of polymerases
... With the great availability of sequences from RNA- and DNAof DNA-dependent DNA polymerase, hereafter called the pol dependent RNA and DNA polymerases, it has become possible /3 type, has only two members: DNA polymerase /3 (Matsukage to delineate a few highly conserved regions for various etal., 198 ...
... With the great availability of sequences from RNA- and DNAof DNA-dependent DNA polymerase, hereafter called the pol dependent RNA and DNA polymerases, it has become possible /3 type, has only two members: DNA polymerase /3 (Matsukage to delineate a few highly conserved regions for various etal., 198 ...
Infectivity analysis of two variable DNA B components of Mungbean
... DNA Bs was determined as 150-nt based on the nucleotide sequence homology. The CR contains a putative stemloop structure with the conserved nonanucleotide sequence (5′-TAATATTAC-3′) in the loop (Lazarowitz 1992). This motif contains the nicking site for the initiation of rolling circle replication. ...
... DNA Bs was determined as 150-nt based on the nucleotide sequence homology. The CR contains a putative stemloop structure with the conserved nonanucleotide sequence (5′-TAATATTAC-3′) in the loop (Lazarowitz 1992). This motif contains the nicking site for the initiation of rolling circle replication. ...
Polymorphisms and Genomic Organization of Repetitive
... A highly abundant repetitive DNA sequence family of Arabidopsis, AtCon, is composed of 178-bp tandemly repeated units and is located at the centromeres of all five chromosome pairs. Analysis of multiple copies of AtCon showed 95% conservation of nucleotides, with some alternative bases, and revealed ...
... A highly abundant repetitive DNA sequence family of Arabidopsis, AtCon, is composed of 178-bp tandemly repeated units and is located at the centromeres of all five chromosome pairs. Analysis of multiple copies of AtCon showed 95% conservation of nucleotides, with some alternative bases, and revealed ...
Effect of non-histone proteins on thermal transition of chromatin and
... to pure DNA. It is concluded that chromatin non-histone proteins contain different fractions of proteins which are causing stabilizing and destabilizing effects on DNA structure. INTRODUCTION Native eukaryotic chromatin contains DNA, RNA, histones and nonhistones proteins (NHP). It was suggested tha ...
... to pure DNA. It is concluded that chromatin non-histone proteins contain different fractions of proteins which are causing stabilizing and destabilizing effects on DNA structure. INTRODUCTION Native eukaryotic chromatin contains DNA, RNA, histones and nonhistones proteins (NHP). It was suggested tha ...
Chapter 10 Review
... resembling a spiral staircase. Each nucleotide consists of a sugar molecule, a phosphate group, and one of four possible bases. The nucleotides are connected to each other by covalent bonds between the sugar of one nucleotide and the phosphate group of the next nucleotide. The double helix arrangeme ...
... resembling a spiral staircase. Each nucleotide consists of a sugar molecule, a phosphate group, and one of four possible bases. The nucleotides are connected to each other by covalent bonds between the sugar of one nucleotide and the phosphate group of the next nucleotide. The double helix arrangeme ...
A Eukaryotic SWI2/SNF2 Domain, an Exquisite Detector of Double
... processes of transcription, recombination, repair, and chromatin remodeling, and the SWI2/SNF2 family of DNA-dependent ATPases is known to be involved in these processes (6). Although all members of this family contain seven helicase-related motifs, none of them have been reported to possess helicas ...
... processes of transcription, recombination, repair, and chromatin remodeling, and the SWI2/SNF2 family of DNA-dependent ATPases is known to be involved in these processes (6). Although all members of this family contain seven helicase-related motifs, none of them have been reported to possess helicas ...
Molecular Computing Viability for Solving Computational Problems
... output) based on a trigger (the input). Logical assembly using DNA: The second approach to demonstrate DNA’s capability of binary logic uses as its input, combinations of different DNA assemblies that can further self assemble according to the input logic into different kinds of superarchitecture th ...
... output) based on a trigger (the input). Logical assembly using DNA: The second approach to demonstrate DNA’s capability of binary logic uses as its input, combinations of different DNA assemblies that can further self assemble according to the input logic into different kinds of superarchitecture th ...
Learning to Communicate in Science and Engineering: Case
... teaching and learning, communication activities are still occurring within the aegis of the classroom or school-based laboratory. In many of these rhetorical situations, the classroom teacher is the ultimate reader and evaluator of students’ texts, and the strongest identities that students assert a ...
... teaching and learning, communication activities are still occurring within the aegis of the classroom or school-based laboratory. In many of these rhetorical situations, the classroom teacher is the ultimate reader and evaluator of students’ texts, and the strongest identities that students assert a ...
Conformation and Rigidity of DNA Microcircles Containing waf1
... to be involved in cell-cycle regulation. In response to DNA damage, it brings about either cell-cycle arrest at G1/S phase or apoptosis through a cascade of events. These events rely primarily on the ability of p53 to function as a transcription factor, which in turn is closely tied with its DNA-bin ...
... to be involved in cell-cycle regulation. In response to DNA damage, it brings about either cell-cycle arrest at G1/S phase or apoptosis through a cascade of events. These events rely primarily on the ability of p53 to function as a transcription factor, which in turn is closely tied with its DNA-bin ...
101. The Role of Rigidity in DNA Looping
... shown that, in the absence of arabinose, the dimeric AraC protein prefers binding to the well separated I1 and O2 half-sites and forming a DNA loop (1–4), Fig. 1. On the addition of arabinose, the protein’s affinity for the I1 and I2 half-sites increases by about 50-fold, leading the protein to pref ...
... shown that, in the absence of arabinose, the dimeric AraC protein prefers binding to the well separated I1 and O2 half-sites and forming a DNA loop (1–4), Fig. 1. On the addition of arabinose, the protein’s affinity for the I1 and I2 half-sites increases by about 50-fold, leading the protein to pref ...
I n o v
... methods for the simultaneous determination of adenine, guanine, and thymine [14]. The MWCNTs composite film enhances surface coverage concentration, increase the electron transfer rate and also exhibits promising enhanced electrocatalytic activity towards the mixture of the biochemical compoundsThe ...
... methods for the simultaneous determination of adenine, guanine, and thymine [14]. The MWCNTs composite film enhances surface coverage concentration, increase the electron transfer rate and also exhibits promising enhanced electrocatalytic activity towards the mixture of the biochemical compoundsThe ...
Vol. 75, No. 5, May 2002 New Laws Reflect the Power and Potential
... Wisconsin's first DNA statutes, however, were written when RFLP ruled the field. The original provision, Wis. Stat. section 972.11(5), addressed admissibility but defined a DNA profile only as an analysis that uses the RFLP analysis; it did not recognize PCR/STR profiling or other techniques. 2001 W ...
... Wisconsin's first DNA statutes, however, were written when RFLP ruled the field. The original provision, Wis. Stat. section 972.11(5), addressed admissibility but defined a DNA profile only as an analysis that uses the RFLP analysis; it did not recognize PCR/STR profiling or other techniques. 2001 W ...
Volume 13 Number 3 A review of DNA repair and possible
... The mechanisms that underlie DNA repair have been extensively explicated in recent years. A few recent discoveries in the field DNA repair enzymes follow: Modrich found that a protein called PCNA is clamped onto the DNA at the strand break. This PCNA, together with the protein that clamps PCNA onto ...
... The mechanisms that underlie DNA repair have been extensively explicated in recent years. A few recent discoveries in the field DNA repair enzymes follow: Modrich found that a protein called PCNA is clamped onto the DNA at the strand break. This PCNA, together with the protein that clamps PCNA onto ...
From Gene to Protein—A Historical Perspective - AP Central
... life, and the storage and transfer of this information are necessary for life to continue. In most cases, this information is passed from parent to offspring via deoxyribonucleic acid, or DNA. This double-stranded molecule provides a simple and elegant solution for the transmission of heritable info ...
... life, and the storage and transfer of this information are necessary for life to continue. In most cases, this information is passed from parent to offspring via deoxyribonucleic acid, or DNA. This double-stranded molecule provides a simple and elegant solution for the transmission of heritable info ...
Identification of Saccharomyces cerevisiae DNA ligase IV
... restriction enzyme digestion: a function which cannot be complemented by CDC9. Lig4p acts in the same DNA repair pathway as the DNA end-binding protein Ku. However, unlike Ku, it does not function in telomere length homeostasis. These findings indicate diversification of function between different e ...
... restriction enzyme digestion: a function which cannot be complemented by CDC9. Lig4p acts in the same DNA repair pathway as the DNA end-binding protein Ku. However, unlike Ku, it does not function in telomere length homeostasis. These findings indicate diversification of function between different e ...
Protein–DNA Hydrophobic Recognition in the Minor Groove
... Keywords: protein– DNA recognition; DNA deformability; hydrophobic interactions; sugar pucker; DNA accessible surface ...
... Keywords: protein– DNA recognition; DNA deformability; hydrophobic interactions; sugar pucker; DNA accessible surface ...
dissertationes biologicae universitatis tartuensis 118
... involved in replication, recombination, repair and transposition. RNA helicases are involved in translation, splicing and ribosome biogenesis. RNA/ DNA helicases are mostly involved in transcription, but also in telomere biogenesis. Helicases interact with single-stranded nucleic acids and move alon ...
... involved in replication, recombination, repair and transposition. RNA helicases are involved in translation, splicing and ribosome biogenesis. RNA/ DNA helicases are mostly involved in transcription, but also in telomere biogenesis. Helicases interact with single-stranded nucleic acids and move alon ...
Integrating multi-scale data on homologous recombination into a
... that is insensitive to dsDNA length and ATP hydrolysis, but dependent on temperature. At 37◦ C the characteristic rate is ∼ 6 bp/s, whereas at 22◦ C it is ∼2 bp/s (8–10). The division of homology recognition into stages where the first stage tests ∼8 bp is consistent with measurements suggesting tha ...
... that is insensitive to dsDNA length and ATP hydrolysis, but dependent on temperature. At 37◦ C the characteristic rate is ∼ 6 bp/s, whereas at 22◦ C it is ∼2 bp/s (8–10). The division of homology recognition into stages where the first stage tests ∼8 bp is consistent with measurements suggesting tha ...
Relationship between Folding and Function in a Sequence
... with high affinity for a variety of targets including the duplex DNAs recognized by GCN4 and CREB (4, 5) and the Q50K engrailed homeodomain (7), the antiapoptotic proteins Bcl-XL (6) and Bcl-2 (10), the oncoprotein hDM2 (manuscript in preparation), the coactivator CBP (8), the EVH1 domain in Mena (9 ...
... with high affinity for a variety of targets including the duplex DNAs recognized by GCN4 and CREB (4, 5) and the Q50K engrailed homeodomain (7), the antiapoptotic proteins Bcl-XL (6) and Bcl-2 (10), the oncoprotein hDM2 (manuscript in preparation), the coactivator CBP (8), the EVH1 domain in Mena (9 ...
DNA Methyltransferases – Role and Function
... DNA methyltransferases were initially discovered as parts of restriction/modification (RM) systems (Arber and Dussoix 1962). S-Adenosyl-L-methionine (AdoMet)dependent DNA and RNA methylation activity was first described by Gold in 1963 (Gold et al. 1963) and a series of papers published by Gold in 1 ...
... DNA methyltransferases were initially discovered as parts of restriction/modification (RM) systems (Arber and Dussoix 1962). S-Adenosyl-L-methionine (AdoMet)dependent DNA and RNA methylation activity was first described by Gold in 1963 (Gold et al. 1963) and a series of papers published by Gold in 1 ...
DNA replication
DNA replication is the process of producing two identical replicas from one original DNA molecule. This biological process occurs in all living organisms and is the basis for biological inheritance. DNA is made up of two strands and each strand of the original DNA molecule serves as a template for the production of the complementary strand, a process referred to as semiconservative replication. Cellular proofreading and error-checking mechanisms ensure near perfect fidelity for DNA replication.In a cell, DNA replication begins at specific locations, or origins of replication, in the genome. Unwinding of DNA at the origin and synthesis of new strands results in replication forks growing bidirectional from the origin. A number of proteins are associated with the replication fork which helps in terms of the initiation and continuation of DNA synthesis. Most prominently, DNA polymerase synthesizes the new DNA by adding complementary nucleotides to the template strand.DNA replication can also be performed in vitro (artificially, outside a cell). DNA polymerases isolated from cells and artificial DNA primers can be used to initiate DNA synthesis at known sequences in a template DNA molecule. The polymerase chain reaction (PCR), a common laboratory technique, cyclically applies such artificial synthesis to amplify a specific target DNA fragment from a pool of DNA.