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Chapter 8 Microbial Genetics
Chapter 8 Microbial Genetics

... Genetic Transfer in Bacteria Genetic transfer-results in genetic variation ...
Molecular Bio Questions2
Molecular Bio Questions2

... 12) Which statement below is FALSE regarding replication of the bacterial chromosome? a) Replication occurs in a bidirectional manner from the origin of replication. b) The leading strand is replicated in the 5’ to 3’ direction and the lagging strand in the 3’ to 5’ direction. c) The primase adds a ...
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...  DNA polymerase III makes strand building in the 5’--3’ direction.  DNA polymerase I replaces the primer  For lagging strand, DNA ligase joins fragments  Telomeres are present at the end of the strand so DNA doesn’t shorten  DNA is proof read for mistakes ...
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... Priming for DNA Replication: Before DNA polymerase can begin work on the daughter strands, a primer must be laid first. Which enzyme produces the primer? What type of molecule is the primer? Synthesis of the new DNA strands: Once the RNA primers are in place, DNA polymerase can go to work. DNA polym ...
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... Color the phosphates blue. Color the sugars (deoxyribose) red. Label one hydrogen bond. Part II. Answer the following questions: 1. Cytosine, guanine, thymine, and adenine are referred to as __________________ bases. 2. Cytosine is always paired with ________________. 3. Adenine is always paired wit ...
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DNA Fingerprinting lab
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dna vaccines - WordPress.com
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... polyadenylation sequences and the production unit composed of bacterial sequences necessary for plasmid amplification and selection. The construction of bacterial plasmids with vaccine inserts is accomplished using recombinant DNA technology. Once constructed, the vaccine plasmid is transformed into ...
Protein Synthesis Lab - Northwest ISD Moodle
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A Model of DNA Objective: To construct a model of double

... Materials: cardboard tube from paper-towel roll, 10 toothpicks, felt-tip markers (two colors), thumbtack, metric ruler (You could use other materials…get inventive!) Procedure: Create a 3-D “live” model of DNA with Sugars and Phosphates labeled. (Here is one way) 1. The typical tube has a seam that ...
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DNA replication



DNA replication is the process of producing two identical replicas from one original DNA molecule. This biological process occurs in all living organisms and is the basis for biological inheritance. DNA is made up of two strands and each strand of the original DNA molecule serves as a template for the production of the complementary strand, a process referred to as semiconservative replication. Cellular proofreading and error-checking mechanisms ensure near perfect fidelity for DNA replication.In a cell, DNA replication begins at specific locations, or origins of replication, in the genome. Unwinding of DNA at the origin and synthesis of new strands results in replication forks growing bidirectional from the origin. A number of proteins are associated with the replication fork which helps in terms of the initiation and continuation of DNA synthesis. Most prominently, DNA polymerase synthesizes the new DNA by adding complementary nucleotides to the template strand.DNA replication can also be performed in vitro (artificially, outside a cell). DNA polymerases isolated from cells and artificial DNA primers can be used to initiate DNA synthesis at known sequences in a template DNA molecule. The polymerase chain reaction (PCR), a common laboratory technique, cyclically applies such artificial synthesis to amplify a specific target DNA fragment from a pool of DNA.
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