Exam3-1406_Spring'06.doc
... B) adenine. C) guanine. D) cytosine. E) uracil. 34) Which occurs in the nucleus? A) transcription only B) assembly of amino acids into protein C) replication of genetic material D) transcription and replication of genetic material E) translation only 35) The anticodon for AUC is A) TAG. B) AUC. C) G ...
... B) adenine. C) guanine. D) cytosine. E) uracil. 34) Which occurs in the nucleus? A) transcription only B) assembly of amino acids into protein C) replication of genetic material D) transcription and replication of genetic material E) translation only 35) The anticodon for AUC is A) TAG. B) AUC. C) G ...
Genetics I Exam 5 Review Sheet - Poultry Science
... 89. Describe how gel electrophoresis works. Do smaller or larger DNA fragments move through the gel faster? What charge is DNA? So where would you place the cathode (negative charge) and the anode (positive charge) in relation to a gel with DNA samples placed in the wells? 90. How are the bands on t ...
... 89. Describe how gel electrophoresis works. Do smaller or larger DNA fragments move through the gel faster? What charge is DNA? So where would you place the cathode (negative charge) and the anode (positive charge) in relation to a gel with DNA samples placed in the wells? 90. How are the bands on t ...
Checklist unit 15: The Chromosomal Basis of Inheritance
... from each other have a higher probability of being sorted independently than genes that are in close proximity of each other (which will, more often than not, be sorted together). The latter are referred to as “linked genes.” Genes found on sex chromosomes have altered inheritance patterns because t ...
... from each other have a higher probability of being sorted independently than genes that are in close proximity of each other (which will, more often than not, be sorted together). The latter are referred to as “linked genes.” Genes found on sex chromosomes have altered inheritance patterns because t ...
Last Name: First Name: Per. _____ Parent Signature: Pre
... up viral DNA. Researchers use them to cut DNA into fragments to create DNA fingerprints in gel electrophoresis and to insert foreign genes when making recombinant DNA ...
... up viral DNA. Researchers use them to cut DNA into fragments to create DNA fingerprints in gel electrophoresis and to insert foreign genes when making recombinant DNA ...
Chromosomes, DNA, and Genes
... 1. DNA is a recipe for _____________. 2. What is a gene? 3. How many genes does a chromosome hold? 4. Where are chromosomes stored in the cell? 5. How many chromosomes do humans have? 6. What organism has the most chromosomes? 7. What organism has the least chromosomes? 8. How many sex chromosomes d ...
... 1. DNA is a recipe for _____________. 2. What is a gene? 3. How many genes does a chromosome hold? 4. Where are chromosomes stored in the cell? 5. How many chromosomes do humans have? 6. What organism has the most chromosomes? 7. What organism has the least chromosomes? 8. How many sex chromosomes d ...
Chromosomes, DNA, and Genes
... 4. Where are chromosomes stored in the cell? 5. How many chromosomes do humans have? 6. What organism has the most chromosomes? 7. What organism has the least chromosomes? 8. How many sex chromosomes do humans have? ...
... 4. Where are chromosomes stored in the cell? 5. How many chromosomes do humans have? 6. What organism has the most chromosomes? 7. What organism has the least chromosomes? 8. How many sex chromosomes do humans have? ...
High Resolution Melt: species identification in theory and practice
... HRM is most conveniently performed as closed tube experiment after the PCR. However, originally this method was developed for a separate HRM-machine manufactured by Idaho Technology which is incapable to perform PCR amplification. Nowadays many block-based quantitative PCR machines are equipped with ...
... HRM is most conveniently performed as closed tube experiment after the PCR. However, originally this method was developed for a separate HRM-machine manufactured by Idaho Technology which is incapable to perform PCR amplification. Nowadays many block-based quantitative PCR machines are equipped with ...
DNA Replication - Living Environment H: 8(A,C)
... DNA Replication • The two strands of DNA unwind or “unzip” breaking the hydrogen bonds and separating. Then each strand becomes the guide or “template” for the making of a new strand. • A protein called an enzyme called DNA polymerase breaks the nitrogen base bonds and the two strands of DNA separa ...
... DNA Replication • The two strands of DNA unwind or “unzip” breaking the hydrogen bonds and separating. Then each strand becomes the guide or “template” for the making of a new strand. • A protein called an enzyme called DNA polymerase breaks the nitrogen base bonds and the two strands of DNA separa ...
16Discovery Of DNA
... • Hershey and Chase found that when the bacteria had been infected with T2 phages that contained radiolabeled proteins, most of the radioactivity was in the supernatant (shell), not in the pellet (core material. • When they examined the bacterial cultures with T2 phage that had radio-labeled DNA, m ...
... • Hershey and Chase found that when the bacteria had been infected with T2 phages that contained radiolabeled proteins, most of the radioactivity was in the supernatant (shell), not in the pellet (core material. • When they examined the bacterial cultures with T2 phage that had radio-labeled DNA, m ...
DNA 1. Evidence for DNA as the genetic material.
... The strands are separated based on length using the technique of gel electrophoresis. In this technique, electricity is passed through a slab of gel into which the strands have been placed. The strands separate because shorter segments travel through the gel faster than longer segments. These fragme ...
... The strands are separated based on length using the technique of gel electrophoresis. In this technique, electricity is passed through a slab of gel into which the strands have been placed. The strands separate because shorter segments travel through the gel faster than longer segments. These fragme ...
here
... – I added DNA polymerase III and dNTPs. – I added very specific DNA primers that mapped onto a specific gene. – Then I started heating and cooling the DNA over and over and over and over…. ...
... – I added DNA polymerase III and dNTPs. – I added very specific DNA primers that mapped onto a specific gene. – Then I started heating and cooling the DNA over and over and over and over…. ...
Polygenic Traits
... – 95% of non-disjunctions occur with the ovum. • Most Down syndrome babies are born to women younger than 35 because those are the ages that most women have children. • Dogma: all your oocytes are present at birth; meiosis is arrested in Prophase I and not completed until adulthood, once a month. – ...
... – 95% of non-disjunctions occur with the ovum. • Most Down syndrome babies are born to women younger than 35 because those are the ages that most women have children. • Dogma: all your oocytes are present at birth; meiosis is arrested in Prophase I and not completed until adulthood, once a month. – ...
DNA - APBioPMWest
... Copy DNA without plasmids? PCR! Polymerase Chain Reaction method for making many, many copies of a specific segment of DNA ~only need 1 cell of DNA to start ...
... Copy DNA without plasmids? PCR! Polymerase Chain Reaction method for making many, many copies of a specific segment of DNA ~only need 1 cell of DNA to start ...
Geometrical ordering of DNA in bacteria
... blob diameters but in all cases 2,000 blobs are used to represent the chromosome (of length 4.02 Mbp) of a C. crescentus swarmer cell. The implemented cell volume has height H = 2 μm and cross section 0.5 μm x 0.5 μm. The fixed positions of ori and ter (at zori = 0.1H and z ter = 0.9H) have been adj ...
... blob diameters but in all cases 2,000 blobs are used to represent the chromosome (of length 4.02 Mbp) of a C. crescentus swarmer cell. The implemented cell volume has height H = 2 μm and cross section 0.5 μm x 0.5 μm. The fixed positions of ori and ter (at zori = 0.1H and z ter = 0.9H) have been adj ...
Comparative genomic hybridization
Comparative genomic hybridization is a molecular cytogenetic method for analysing copy number variations (CNVs) relative to ploidy level in the DNA of a test sample compared to a reference sample, without the need for culturing cells. The aim of this technique is to quickly and efficiently compare two genomic DNA samples arising from two sources, which are most often closely related, because it is suspected that they contain differences in terms of either gains or losses of either whole chromosomes or subchromosomal regions (a portion of a whole chromosome). This technique was originally developed for the evaluation of the differences between the chromosomal complements of solid tumor and normal tissue, and has an improved resoIution of 5-10 megabases compared to the more traditional cytogenetic analysis techniques of giemsa banding and fluorescence in situ hybridization (FISH) which are limited by the resolution of the microscope utilized.This is achieved through the use of competitive fluorescence in situ hybridization. In short, this involves the isolation of DNA from the two sources to be compared, most commonly a test and reference source, independent labelling of each DNA sample with a different fluorophores (fluorescent molecules) of different colours (usually red and green), denaturation of the DNA so that it is single stranded, and the hybridization of the two resultant samples in a 1:1 ratio to a normal metaphase spread of chromosomes, to which the labelled DNA samples will bind at their locus of origin. Using a fluorescence microscope and computer software, the differentially coloured fluorescent signals are then compared along the length of each chromosome for identification of chromosomal differences between the two sources. A higher intensity of the test sample colour in a specific region of a chromosome indicates the gain of material of that region in the corresponding source sample, while a higher intensity of the reference sample colour indicates the loss of material in the test sample in that specific region. A neutral colour (yellow when the fluorophore labels are red and green) indicates no difference between the two samples in that location.CGH is only able to detect unbalanced chromosomal abnormalities. This is because balanced chromosomal abnormalities such as reciprocal translocations, inversions or ring chromosomes do not affect copy number, which is what is detected by CGH technologies. CGH does, however, allow for the exploration of all 46 human chromosomes in single test and the discovery of deletions and duplications, even on the microscopic scale which may lead to the identification of candidate genes to be further explored by other cytological techniques.Through the use of DNA microarrays in conjunction with CGH techniques, the more specific form of array CGH (aCGH) has been developed, allowing for a locus-by-locus measure of CNV with increased resolution as low as 100 kilobases. This improved technique allows for the aetiology of known and unknown conditions to be discovered.