Restriction enzyme
... It is a fragment of DNA of variable length (usually 100-1000 bases long), which is used to detect in DNA the presence of nucleotide sequences that are complementary to the sequence in the probe Must be labeled to be visualized Usually prepared by making a radioactive copy of a DNA fragment. Probing ...
... It is a fragment of DNA of variable length (usually 100-1000 bases long), which is used to detect in DNA the presence of nucleotide sequences that are complementary to the sequence in the probe Must be labeled to be visualized Usually prepared by making a radioactive copy of a DNA fragment. Probing ...
Chapter 13
... Cohen and Boyer eliminated the bacteria that did not contain the recombinant then they needed to test each clone to see if the frog rRNA gene was indeed present. The procedure for identifying a specific gene is known as the Southern blot The Southern blot is a technique that uses radioactively label ...
... Cohen and Boyer eliminated the bacteria that did not contain the recombinant then they needed to test each clone to see if the frog rRNA gene was indeed present. The procedure for identifying a specific gene is known as the Southern blot The Southern blot is a technique that uses radioactively label ...
lecture15
... The DNA polymerase of T7 bacteriophage has DNA polymerase and 3' -> 5' exonuclease activities, but lacks a 5' -> 3' exonuclease domain. It is thus very similar in activity to Klenow fragment and T4 DNA polymerase. The claim to fame for T7 DNA polymerase is it's processivity. That is to say, the aver ...
... The DNA polymerase of T7 bacteriophage has DNA polymerase and 3' -> 5' exonuclease activities, but lacks a 5' -> 3' exonuclease domain. It is thus very similar in activity to Klenow fragment and T4 DNA polymerase. The claim to fame for T7 DNA polymerase is it's processivity. That is to say, the aver ...
Clone
... oligonuceotide probes from a known amino acid sequence. A radioactively labeled set of DNA (degenerate) oligonucleotides ...
... oligonuceotide probes from a known amino acid sequence. A radioactively labeled set of DNA (degenerate) oligonucleotides ...
Mitosis Meiosis Notes
... a. twice as many males as females have this. b. there is a region on the X chromosome that has many replications which elongates the X chromosome. Family history of Fragile X syndrome, especially a male relative Mental retardation Large testicles after puberty Large body size Tendency to avoid eye c ...
... a. twice as many males as females have this. b. there is a region on the X chromosome that has many replications which elongates the X chromosome. Family history of Fragile X syndrome, especially a male relative Mental retardation Large testicles after puberty Large body size Tendency to avoid eye c ...
File - NCEA Level 3 Biology
... amino acids and the order they are arranged in. This is a universal code, so in theory the same protein can be made by any organism. – The promoter region – controls gene expression. Regulates in which tissue the gene should be expressed, at what time and in response to what stimulus the gene is ...
... amino acids and the order they are arranged in. This is a universal code, so in theory the same protein can be made by any organism. – The promoter region – controls gene expression. Regulates in which tissue the gene should be expressed, at what time and in response to what stimulus the gene is ...
Unit 6: Biotechnology
... DNA Information Is Not Private As the court recognized, the U.S. Department of Defense had good reasons for requiring that DNA samples be taken and stored. Furthermore, DNA sequences are no more private and personal than fingerprints or photographs, which are taken by private and government agencies ...
... DNA Information Is Not Private As the court recognized, the U.S. Department of Defense had good reasons for requiring that DNA samples be taken and stored. Furthermore, DNA sequences are no more private and personal than fingerprints or photographs, which are taken by private and government agencies ...
Model of unequal chromosomal crossing over in DNA sequences1
... parental chromosome changes in length, one becomes longer, while the other becomes shorter. We base our model on this mechanism of unequal chromosomal crossing over, which is de ned as follows: Model. Consider a segment with a DTR of length ‘ (see Fig. 2). We de ne unequal crossing over to be when a ...
... parental chromosome changes in length, one becomes longer, while the other becomes shorter. We base our model on this mechanism of unequal chromosomal crossing over, which is de ned as follows: Model. Consider a segment with a DTR of length ‘ (see Fig. 2). We de ne unequal crossing over to be when a ...
lecture_11(LP)
... genomic DNA library had been made from ade2 mutant yeast? no colonies on the -adenine plate 3. Why is this type of functional cloning considered an example of “complementation”? 4. Can this experiment be done using the human “ADE2” ...
... genomic DNA library had been made from ade2 mutant yeast? no colonies on the -adenine plate 3. Why is this type of functional cloning considered an example of “complementation”? 4. Can this experiment be done using the human “ADE2” ...
Uracil-DNA Glycosylase (UDG)
... temperature (95°C) under alkaline conditions (during the initial denaturation step) will remove contaminating U-DNA from the sample. This method, of course, requires that all PCR-reactions in the lab have to be carried out with dUTP instead of dTTP step ...
... temperature (95°C) under alkaline conditions (during the initial denaturation step) will remove contaminating U-DNA from the sample. This method, of course, requires that all PCR-reactions in the lab have to be carried out with dUTP instead of dTTP step ...
Ch. 12 Quiz! Get Out A Piece of Paper!
... c) prokaryotic DNA is replicated in only one direction d) eukaryotic DNA takes much longer to replicate ...
... c) prokaryotic DNA is replicated in only one direction d) eukaryotic DNA takes much longer to replicate ...
Isolating, Cloning and Sequencing DNA
... Technique by which charged molecules are separated basrd on their size or charge, by passing them through a gel within an electric field ...
... Technique by which charged molecules are separated basrd on their size or charge, by passing them through a gel within an electric field ...
A conserved repetitive DNA element located in the centromeres of
... species are much more complex compared with those from budding yeast. These centromeres, called regional centromeres, encompass kilobases or megabases of DNA and include both unique and repetitive DNA sequences. Several different repetitive DNA elements were identified in the centromeres of fission ...
... species are much more complex compared with those from budding yeast. These centromeres, called regional centromeres, encompass kilobases or megabases of DNA and include both unique and repetitive DNA sequences. Several different repetitive DNA elements were identified in the centromeres of fission ...
DNA Questions #4 Questions on the PCR Process:
... person and sample are the same. If the sample is from a female, it will show only one peak for AMELX at 109 bp. If the sample is from a male, there will be two peaks. One will be for AMELX at 109 bp and one for AMELY at 112 bp. The two peaks will be very close to each other at the beginning of the e ...
... person and sample are the same. If the sample is from a female, it will show only one peak for AMELX at 109 bp. If the sample is from a male, there will be two peaks. One will be for AMELX at 109 bp and one for AMELY at 112 bp. The two peaks will be very close to each other at the beginning of the e ...
Sodium Bisulfite Methods
... Rauch TA and Pfeifer GP. 2009. Chapter 9: Methods for Assessing Genome Wide DNA methylation. In: Handbook of Epigenetics : The New Molecular and Medical Genetics. ed. Tollefsbol T. Academic Press. Rauch TA et al. 2006. MIRA-assisted microarray analysis, a new technology for the determination of DNA ...
... Rauch TA and Pfeifer GP. 2009. Chapter 9: Methods for Assessing Genome Wide DNA methylation. In: Handbook of Epigenetics : The New Molecular and Medical Genetics. ed. Tollefsbol T. Academic Press. Rauch TA et al. 2006. MIRA-assisted microarray analysis, a new technology for the determination of DNA ...
7. Biotechnology- Using Molecular Biology and Genetic Engineering
... In order for selective breeding to be successful, there must be a lot of genetic variation in the population Breeders increase the genetic variation in a population by inducing mutations, which are the ultimate source of genetic ...
... In order for selective breeding to be successful, there must be a lot of genetic variation in the population Breeders increase the genetic variation in a population by inducing mutations, which are the ultimate source of genetic ...
Chromosome - Rajshahi University
... 4.Metacentric : p and q arms are exactly the same length; centromere in exact middle of chromosome ...
... 4.Metacentric : p and q arms are exactly the same length; centromere in exact middle of chromosome ...
Chapter 10: Biotechnology
... in 1,000,000,000,000,000,000. This is one in a quintillion, which is much more than the number of people that are even alive on Earth!!! • Usually, a standard set of thirteen short tandem repeat regions is used to make a DNA fingerprint of an individual if it is to be used in any court in the U.S. • ...
... in 1,000,000,000,000,000,000. This is one in a quintillion, which is much more than the number of people that are even alive on Earth!!! • Usually, a standard set of thirteen short tandem repeat regions is used to make a DNA fingerprint of an individual if it is to be used in any court in the U.S. • ...
Comparative genomic hybridization
Comparative genomic hybridization is a molecular cytogenetic method for analysing copy number variations (CNVs) relative to ploidy level in the DNA of a test sample compared to a reference sample, without the need for culturing cells. The aim of this technique is to quickly and efficiently compare two genomic DNA samples arising from two sources, which are most often closely related, because it is suspected that they contain differences in terms of either gains or losses of either whole chromosomes or subchromosomal regions (a portion of a whole chromosome). This technique was originally developed for the evaluation of the differences between the chromosomal complements of solid tumor and normal tissue, and has an improved resoIution of 5-10 megabases compared to the more traditional cytogenetic analysis techniques of giemsa banding and fluorescence in situ hybridization (FISH) which are limited by the resolution of the microscope utilized.This is achieved through the use of competitive fluorescence in situ hybridization. In short, this involves the isolation of DNA from the two sources to be compared, most commonly a test and reference source, independent labelling of each DNA sample with a different fluorophores (fluorescent molecules) of different colours (usually red and green), denaturation of the DNA so that it is single stranded, and the hybridization of the two resultant samples in a 1:1 ratio to a normal metaphase spread of chromosomes, to which the labelled DNA samples will bind at their locus of origin. Using a fluorescence microscope and computer software, the differentially coloured fluorescent signals are then compared along the length of each chromosome for identification of chromosomal differences between the two sources. A higher intensity of the test sample colour in a specific region of a chromosome indicates the gain of material of that region in the corresponding source sample, while a higher intensity of the reference sample colour indicates the loss of material in the test sample in that specific region. A neutral colour (yellow when the fluorophore labels are red and green) indicates no difference between the two samples in that location.CGH is only able to detect unbalanced chromosomal abnormalities. This is because balanced chromosomal abnormalities such as reciprocal translocations, inversions or ring chromosomes do not affect copy number, which is what is detected by CGH technologies. CGH does, however, allow for the exploration of all 46 human chromosomes in single test and the discovery of deletions and duplications, even on the microscopic scale which may lead to the identification of candidate genes to be further explored by other cytological techniques.Through the use of DNA microarrays in conjunction with CGH techniques, the more specific form of array CGH (aCGH) has been developed, allowing for a locus-by-locus measure of CNV with increased resolution as low as 100 kilobases. This improved technique allows for the aetiology of known and unknown conditions to be discovered.