Low cost flatbed scanner label-free biosensor - bu people
... addition, they have also been adapted for fluorescence microscopy8 and digital holographic microscopy9 applications. In recent years, several biosensors that rely on label-free detection have been introduced, which are suitable for lowresource settings as they reduce sample preparation and washing s ...
... addition, they have also been adapted for fluorescence microscopy8 and digital holographic microscopy9 applications. In recent years, several biosensors that rely on label-free detection have been introduced, which are suitable for lowresource settings as they reduce sample preparation and washing s ...
Chromosomal Amplification Is Associated with
... chromosomal amplification in GCTs associated with resistance. Among the other sites amplified that were restricted to resistant tumors, several are of further interest. The ßCi-2-relatedgene, MFL1/ and overexpressed (64%) in breast cancer though its role in tumor BCL2AÌ,has been mapped to 15q23-24 ...
... chromosomal amplification in GCTs associated with resistance. Among the other sites amplified that were restricted to resistant tumors, several are of further interest. The ßCi-2-relatedgene, MFL1/ and overexpressed (64%) in breast cancer though its role in tumor BCL2AÌ,has been mapped to 15q23-24 ...
Exercise - GEP Community Server
... The first stop is Find Repeats. During this stop the submitted DNA sequence is scanned for repetitive sequences using the RepeatMasker computer script. It is essential that the search for genes is done in regions that do not contain repetitive DNA. For a large genome with lots of repetitive DNA, th ...
... The first stop is Find Repeats. During this stop the submitted DNA sequence is scanned for repetitive sequences using the RepeatMasker computer script. It is essential that the search for genes is done in regions that do not contain repetitive DNA. For a large genome with lots of repetitive DNA, th ...
IRAP (interretroelement amplified polymorphism)
... analysis provides insights into the nature and evolution of genes (eg resistance genes), tells us about diversity within a crop species and its wild relatives (eg which species and geographical area gave rise to the crop), and allows us to follow and direct selection programmes (eg parent choice and ...
... analysis provides insights into the nature and evolution of genes (eg resistance genes), tells us about diversity within a crop species and its wild relatives (eg which species and geographical area gave rise to the crop), and allows us to follow and direct selection programmes (eg parent choice and ...
FP-123
... quantitation of DNA (Fig. 3). RNA spiking experiments of DNA have shown A260 readings to increase. Additionally, an increase in the A 260/A 280 ratio was observed with increasing RNA contamination. ...
... quantitation of DNA (Fig. 3). RNA spiking experiments of DNA have shown A260 readings to increase. Additionally, an increase in the A 260/A 280 ratio was observed with increasing RNA contamination. ...
TOPICS FOR EXAMINATION II – Biology 1406
... What is biotechnology?. What are restriction endonucleases? What is special about the way they cleave DNA? From which kind of microorganisms are all restriction endonucleases obtained? How will DNA fragments of different sizes migrate through agarose gels, and how will they sort themselves by size? ...
... What is biotechnology?. What are restriction endonucleases? What is special about the way they cleave DNA? From which kind of microorganisms are all restriction endonucleases obtained? How will DNA fragments of different sizes migrate through agarose gels, and how will they sort themselves by size? ...
MBLG1001 Lecture 9 The Flow of Genetic Information Replication
... If replication was dispersive… • Hybrid H:L DNA would result but if the individual strands were analysed under denaturing conditions (in CsCl with NaOH to keep the strands apart) they would also have an intermediate density. • The individual DNA strands would always be completely H or L in the other ...
... If replication was dispersive… • Hybrid H:L DNA would result but if the individual strands were analysed under denaturing conditions (in CsCl with NaOH to keep the strands apart) they would also have an intermediate density. • The individual DNA strands would always be completely H or L in the other ...
Note 8.1 - Cloning DNA
... Restriction enzymes were first discovered by DR. Hamilton Smith in 1970, as he studied bacteria and their ability to resisted viral infections. Since the start of his research there have been 2500 restriction enzymes classified with a 200 target sequences. The primary functions of restriction enzyme ...
... Restriction enzymes were first discovered by DR. Hamilton Smith in 1970, as he studied bacteria and their ability to resisted viral infections. Since the start of his research there have been 2500 restriction enzymes classified with a 200 target sequences. The primary functions of restriction enzyme ...
Chapter 10 Gene Mutation: Origins and Repair Processes
... Salvatoria Luria and Max Delbrück's fluctuation test Hypothesis: random mutation or directed physiological change (1943) ...
... Salvatoria Luria and Max Delbrück's fluctuation test Hypothesis: random mutation or directed physiological change (1943) ...
isolation and sequencing of a genomic dna encoding for ascorbat
... accumulation in melon fruits could be achieved. At least four genes are considered by [6] responsible for AO biosynthesis and three of them (AO1 ; AO2 and AO3) have already been isolated and sequenced by the above cited scientists. The purpose of the present paper was the isolation and characterizat ...
... accumulation in melon fruits could be achieved. At least four genes are considered by [6] responsible for AO biosynthesis and three of them (AO1 ; AO2 and AO3) have already been isolated and sequenced by the above cited scientists. The purpose of the present paper was the isolation and characterizat ...
Genomic Library cDNA Library
... What is a genomic library and why is it important? A genomic library is a collection of cloned sequences which represents the entire genome. It allows the analysis of gene promoters which control how genes function (where and when they are expressed, and in response to which stimuli) ...
... What is a genomic library and why is it important? A genomic library is a collection of cloned sequences which represents the entire genome. It allows the analysis of gene promoters which control how genes function (where and when they are expressed, and in response to which stimuli) ...
Replication 1
... Covalent extension—synthesis of new strand as an extension of an old strand (“Rolling Circle”) ...
... Covalent extension—synthesis of new strand as an extension of an old strand (“Rolling Circle”) ...
Proving that DNA Replication is Semiconservative
... species, corresponding to 15N-labeled DNA. As DNA replication proceeded, the amount of (15N)-DNA decreased, and a second DNA species, consisting of hybrid DNA molecules containing 15N- and 14N-labeled strands, appeared. DNA collected after completion of the first round of replication was found to se ...
... species, corresponding to 15N-labeled DNA. As DNA replication proceeded, the amount of (15N)-DNA decreased, and a second DNA species, consisting of hybrid DNA molecules containing 15N- and 14N-labeled strands, appeared. DNA collected after completion of the first round of replication was found to se ...
DNA sequencing
... DNA is analysed on agarose gels which have big spaces to allow larger molecules of DNA to ...
... DNA is analysed on agarose gels which have big spaces to allow larger molecules of DNA to ...
Comparative genomic hybridization
Comparative genomic hybridization is a molecular cytogenetic method for analysing copy number variations (CNVs) relative to ploidy level in the DNA of a test sample compared to a reference sample, without the need for culturing cells. The aim of this technique is to quickly and efficiently compare two genomic DNA samples arising from two sources, which are most often closely related, because it is suspected that they contain differences in terms of either gains or losses of either whole chromosomes or subchromosomal regions (a portion of a whole chromosome). This technique was originally developed for the evaluation of the differences between the chromosomal complements of solid tumor and normal tissue, and has an improved resoIution of 5-10 megabases compared to the more traditional cytogenetic analysis techniques of giemsa banding and fluorescence in situ hybridization (FISH) which are limited by the resolution of the microscope utilized.This is achieved through the use of competitive fluorescence in situ hybridization. In short, this involves the isolation of DNA from the two sources to be compared, most commonly a test and reference source, independent labelling of each DNA sample with a different fluorophores (fluorescent molecules) of different colours (usually red and green), denaturation of the DNA so that it is single stranded, and the hybridization of the two resultant samples in a 1:1 ratio to a normal metaphase spread of chromosomes, to which the labelled DNA samples will bind at their locus of origin. Using a fluorescence microscope and computer software, the differentially coloured fluorescent signals are then compared along the length of each chromosome for identification of chromosomal differences between the two sources. A higher intensity of the test sample colour in a specific region of a chromosome indicates the gain of material of that region in the corresponding source sample, while a higher intensity of the reference sample colour indicates the loss of material in the test sample in that specific region. A neutral colour (yellow when the fluorophore labels are red and green) indicates no difference between the two samples in that location.CGH is only able to detect unbalanced chromosomal abnormalities. This is because balanced chromosomal abnormalities such as reciprocal translocations, inversions or ring chromosomes do not affect copy number, which is what is detected by CGH technologies. CGH does, however, allow for the exploration of all 46 human chromosomes in single test and the discovery of deletions and duplications, even on the microscopic scale which may lead to the identification of candidate genes to be further explored by other cytological techniques.Through the use of DNA microarrays in conjunction with CGH techniques, the more specific form of array CGH (aCGH) has been developed, allowing for a locus-by-locus measure of CNV with increased resolution as low as 100 kilobases. This improved technique allows for the aetiology of known and unknown conditions to be discovered.