Worksheet for 4/16
... gel electrophoresis. Diagram a gel including electric charge, and labeled fragments. ...
... gel electrophoresis. Diagram a gel including electric charge, and labeled fragments. ...
Biotech
... PCR • This is the polymerase chain reaction. It is a technique to multiply a sample of DNA many times in a short period of time. It supplies the scientist with sufficient DNA for further testing. http://www.dnalc.org/resources/animations/pcr.html ...
... PCR • This is the polymerase chain reaction. It is a technique to multiply a sample of DNA many times in a short period of time. It supplies the scientist with sufficient DNA for further testing. http://www.dnalc.org/resources/animations/pcr.html ...
Karyotyping
... Hybridization (CGH) is a molecularcytogenetic method for the analysis of regional changes in the DNA content of tumor cells. Using epifluorescence microscopy and quantitative image analysis, differences in the fluorescence ratio of the tumor versus that of the control DNA can be detected. The whole ...
... Hybridization (CGH) is a molecularcytogenetic method for the analysis of regional changes in the DNA content of tumor cells. Using epifluorescence microscopy and quantitative image analysis, differences in the fluorescence ratio of the tumor versus that of the control DNA can be detected. The whole ...
Genetic Engineering - Duplin County Schools
... • Allowing only those with desired character istics to produce the next generation ...
... • Allowing only those with desired character istics to produce the next generation ...
Application Sheet: DNA - NETZSCH Thermal Analysis
... and phosphate groups. This backbone carries four types of molecules called bases and it is the sequence of these ...
... and phosphate groups. This backbone carries four types of molecules called bases and it is the sequence of these ...
22. Recombinant DNA Technology
... 1. Heat shock: CaCl2 at 0oC then heat to 37-42oC 2. Electroporation – apply high voltage BAC – 5,000 to 400,000 bp insert ...
... 1. Heat shock: CaCl2 at 0oC then heat to 37-42oC 2. Electroporation – apply high voltage BAC – 5,000 to 400,000 bp insert ...
Microarrays: The Future of Prenatal Genetic Testing
... Molecular evaluation by microarrays has several advantages ...
... Molecular evaluation by microarrays has several advantages ...
array CGH
... contains high-density coverage for ~500 targeted regions with an average oligo probe spacing of ~5 kb or at least 20 probes per gene. These targeted regions include clinically relevant haploinsufficient genes, X-linked intellectual disability genes, all recurrent microdeletion/microduplication syndr ...
... contains high-density coverage for ~500 targeted regions with an average oligo probe spacing of ~5 kb or at least 20 probes per gene. These targeted regions include clinically relevant haploinsufficient genes, X-linked intellectual disability genes, all recurrent microdeletion/microduplication syndr ...
DNA, Genes and Chromosomes
... 2. To obtain a merit you need to use pictures of your DNA model in a flow diagram showing the progression from a cell to a gene writing descriptions. 3. A distinction will be achieved if you produce a poster writing a summary about how genes can be shuffled ...
... 2. To obtain a merit you need to use pictures of your DNA model in a flow diagram showing the progression from a cell to a gene writing descriptions. 3. A distinction will be achieved if you produce a poster writing a summary about how genes can be shuffled ...
Array comparative genomic hybridization (array
... Karyotyping versus array-CGH In principle, both karyotyping and arrays are genome-wide technologies which can be used to assess the presence of genomic imbalance such as CNVs. Although they may look like very different technologies, the primary difference between them is in the resolution, which is ...
... Karyotyping versus array-CGH In principle, both karyotyping and arrays are genome-wide technologies which can be used to assess the presence of genomic imbalance such as CNVs. Although they may look like very different technologies, the primary difference between them is in the resolution, which is ...
SW describe how techniques such as DNA
... Sex-influenced traits are those that are expressed differently in the two sexes. Such traits are autosomal, which means that the genes responsible for their expression are not carried on the sex chromosomes. ...
... Sex-influenced traits are those that are expressed differently in the two sexes. Such traits are autosomal, which means that the genes responsible for their expression are not carried on the sex chromosomes. ...
Array CGH for detection of chromosome imbalance
... “benign” CNVs – published as present in normal individuals and /or common in our population known regions (eg microdeletion syndrome loci) unknown CNVs – not in DGV ...
... “benign” CNVs – published as present in normal individuals and /or common in our population known regions (eg microdeletion syndrome loci) unknown CNVs – not in DGV ...
DNA and Chromosomes
... What is the relationship between DNA, chromosomes, and any organism? Drag and drop the descriptive phrase to the correct column, thereby helping us to describe the relationships between these important components of inheritance. ...
... What is the relationship between DNA, chromosomes, and any organism? Drag and drop the descriptive phrase to the correct column, thereby helping us to describe the relationships between these important components of inheritance. ...
Figure 2 Representation of the steps required for DNA sequence
... Supplementary Figure 1 Representation of the steps required for DNA sequence analysis to detect a germline mutation. Family members of the index case, that is the proband (arrow), are ascertained. After genetic counseling and obtaining informed consent, venous blood samples are collected and leucocy ...
... Supplementary Figure 1 Representation of the steps required for DNA sequence analysis to detect a germline mutation. Family members of the index case, that is the proband (arrow), are ascertained. After genetic counseling and obtaining informed consent, venous blood samples are collected and leucocy ...
Non-Mendelian Genetics Test Review
... What is chromosomal analysis? Chromosomal analysis is a procedure that isolates the chromosome pairs so that they may be visualized to determine abnormalities. ...
... What is chromosomal analysis? Chromosomal analysis is a procedure that isolates the chromosome pairs so that they may be visualized to determine abnormalities. ...
Document
... Used by majority of groups Biopsy at 6-10 cell stage Blastomeres totipotent 1-2 cells for analysis ...
... Used by majority of groups Biopsy at 6-10 cell stage Blastomeres totipotent 1-2 cells for analysis ...
Karyotyping, FISH and CGH array
... of the probes is however the main limitation of FISH: it can detect only the specific DNA sequences to which it is complimentary and to which it can hybridise. ...
... of the probes is however the main limitation of FISH: it can detect only the specific DNA sequences to which it is complimentary and to which it can hybridise. ...
Comparative genomic hybridization
Comparative genomic hybridization is a molecular cytogenetic method for analysing copy number variations (CNVs) relative to ploidy level in the DNA of a test sample compared to a reference sample, without the need for culturing cells. The aim of this technique is to quickly and efficiently compare two genomic DNA samples arising from two sources, which are most often closely related, because it is suspected that they contain differences in terms of either gains or losses of either whole chromosomes or subchromosomal regions (a portion of a whole chromosome). This technique was originally developed for the evaluation of the differences between the chromosomal complements of solid tumor and normal tissue, and has an improved resoIution of 5-10 megabases compared to the more traditional cytogenetic analysis techniques of giemsa banding and fluorescence in situ hybridization (FISH) which are limited by the resolution of the microscope utilized.This is achieved through the use of competitive fluorescence in situ hybridization. In short, this involves the isolation of DNA from the two sources to be compared, most commonly a test and reference source, independent labelling of each DNA sample with a different fluorophores (fluorescent molecules) of different colours (usually red and green), denaturation of the DNA so that it is single stranded, and the hybridization of the two resultant samples in a 1:1 ratio to a normal metaphase spread of chromosomes, to which the labelled DNA samples will bind at their locus of origin. Using a fluorescence microscope and computer software, the differentially coloured fluorescent signals are then compared along the length of each chromosome for identification of chromosomal differences between the two sources. A higher intensity of the test sample colour in a specific region of a chromosome indicates the gain of material of that region in the corresponding source sample, while a higher intensity of the reference sample colour indicates the loss of material in the test sample in that specific region. A neutral colour (yellow when the fluorophore labels are red and green) indicates no difference between the two samples in that location.CGH is only able to detect unbalanced chromosomal abnormalities. This is because balanced chromosomal abnormalities such as reciprocal translocations, inversions or ring chromosomes do not affect copy number, which is what is detected by CGH technologies. CGH does, however, allow for the exploration of all 46 human chromosomes in single test and the discovery of deletions and duplications, even on the microscopic scale which may lead to the identification of candidate genes to be further explored by other cytological techniques.Through the use of DNA microarrays in conjunction with CGH techniques, the more specific form of array CGH (aCGH) has been developed, allowing for a locus-by-locus measure of CNV with increased resolution as low as 100 kilobases. This improved technique allows for the aetiology of known and unknown conditions to be discovered.