• Study Resource
  • Explore Categories
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
BIOLOGY Cells Unit GUIDE SHEET
BIOLOGY Cells Unit GUIDE SHEET

... 14. What are some benefits (PROS) to the use of genetically modified foods (pages 239-240)? ...
slides - István Albert
slides - István Albert

... In  2006  the  Archon  Genomics  X  PRIZE  was  to   award  $10  million  to  the  first  team  to  rapidly,   accurately  and  economically  sequence  100   whole  human  genomes  to  a  level  of  accuracy   never  before  achieved. ...
Portfolio 4 Index
Portfolio 4 Index

... Objective: Infer how traits are inherited over generations by interpreting pedigree charts Instruction: Answer the following questions 1. Explain why some cats have different colors; consider the sex of the cat. ...
DNA Technology and its Applications
DNA Technology and its Applications

...  The technique of removing, changing, or adding genes (chunks of DNA) to a DNA molecule to change the information it contains. By changing this information, we can change the type or amount of proteins an organism is capable of producing. ...
INTEGRATED MICROSYSTEM FOR FORENSIC DNA
INTEGRATED MICROSYSTEM FOR FORENSIC DNA

... addition of an internal size standard to the amplification products and to an allelic ladder. The sample amplification products and the allelic ladder are then separated sequentially by CE. The PCR requires thermal cycling between three temperature zones, ranging from 55 to 950C, and the CE channel ...
DNA, Genes, and Proteins EOC Review Describe the chemical and
DNA, Genes, and Proteins EOC Review Describe the chemical and

... The numbered statements below are the EOC objectives that the state has decided you must be able to meet coming out of Biology I. I have included some sample released item questions from past EOC tests that go with each objective. The purpose is to give you an opportunity to see the types of questio ...
Gene Technology
Gene Technology

... 0 After mRNA has been made in the nucleus 0 Introns are spliced out of the mRNA 0 Exons bind together 0 Addition of 5’ cap and poly A tail ...
Taxonomy
Taxonomy

... method of DNA analysis, developed by Johannes Hedman, a doctoral student at Applied Microbiology. Together with the Swedish National Laboratory of Forensic Science, SKL, he has created a new enzyme combination that makes DNA profiles from crime scene samples clearer. This raises the chances of linki ...
Genome Variant Calling: A sta>s>cal perspec>ve
Genome Variant Calling: A sta>s>cal perspec>ve

... •  the  distribu2ons  of  the  test  sta2s2c  is  discrete   •  the  distribu2ons  of  the  p-­‐values  are  too   •  as  coverage  increases,  for  a  fixed  cut-­‐off,  the   size  of  the  test  decreases   •  our  p-­‐values,  if  aggre ...
Genetic Engineering Includes
Genetic Engineering Includes

... • A technique used by scientists to distinguish between individuals of the same species using only samples of their DNA • Unless they are identical twins, individual organisms all have unique DNA. • The chemical structure of the DNA may be the same (A, T, C & G), but the order of the base pairs is d ...
DNA Review
DNA Review

... • p arm – short arm of chromosome • q arm – long arm • Bands are numbered from centromere outwards to telomere • 15pter – somewhere on terminus of p arm of chromosome 15 • 15q11.3 – exact band and sub-band • Location of DNA markers may be referred to based on location on chromosome ...
Genetic Engineering
Genetic Engineering

... • A technique used by scientists to distinguish between individuals of the same species using only samples of their DNA • Unless they are identical twins, individual organisms all have unique DNA. • The chemical structure of the DNA may be the same (A, T, C & G), but the order of the base pairs is d ...
Genetic Engineering
Genetic Engineering

... • A technique used by scientists to distinguish between individuals of the same species using only samples of their DNA • Unless they are identical twins, individual organisms all have unique DNA. • The chemical structure of the DNA may be the same (A, T, C & G), but the order of the base pairs is d ...
Nucleotide drug targets
Nucleotide drug targets

... The double stranded structure provides protection to the information containing face of the bases, an extra copy of the information and a template for repair. The outside of the DNA, with its predominating phosphate groups and sugar is very hydrophilic. The bases, buried in the interior, are much mo ...
Nucleotide drug targets.
Nucleotide drug targets.

... The double stranded structure provides protection to the information containing face of the bases, an extra copy of the information and a template for repair. The outside of the DNA, with its predominating phosphate groups and sugar is very hydrophilic. The bases, buried in the interior, are much mo ...
Why teach a course in bioinformatics?
Why teach a course in bioinformatics?

DNA: Contamination Control - Sacramento County District Attorney
DNA: Contamination Control - Sacramento County District Attorney

... involve the handling of amplified DNA. This includes quantitation (based on Real Time PCR methodology), capillary electrophoresis of amplified DNA, waste disposal of amplified DNA products, and storage of amplified DNA.  Dedicated equipment and supplies located in this room are for use only with am ...
History_of_DNA
History_of_DNA

... DNA was responsible for transformation How would you design an experiment to prove that DNA was responsible for transformation? ...
Section 2: Figures
Section 2: Figures

... The absorbance of genomic and plasmid DNA were measured at 260 and 280 nm using Beckman DU 730 spectrophotometer. The DNA concentration estimation was based on the extinction coefficient for absorbance at 260 nm (1 cm path length) of 0.02 µg mL-1 or DNA with an absorption 1 = 50µg mL-1. [2] The 260/ ...
Document
Document

... of foreign donor DNA to represent the entire genome of the organism of interest. • Each clone will represent a portion of the genome. • Libraries may use plasmid vectors and host bacteria, or they may use a bacteriophage vector. • The library can then be screened for any gene of interest, and used o ...
Recombinant DNA
Recombinant DNA

... The cells containing the recombinant DNA are grown in culture – replicating the new DNA as they do Not all the cells will be descendants of those with the recombinant DNA  these need to be eliminated from the culture For this reason, plasmids that also confer resistance to a particular antibiotic o ...
RESTRICTION ENZYMES
RESTRICTION ENZYMES

... reconnects phosphodiester bonds. RE Video restriction enzymes.exe ...
Human DNA Dance - University of Wisconsin Biotechnology Center
Human DNA Dance - University of Wisconsin Biotechnology Center

... asking the two lines to release their handshakes and take one step to the left, while keeping their right hands in the C, T, G or A form. You can show how two complementary single strands of DNA can anneal (come together) by then having the two strands come back together into a double-stranded form. ...
General Lecture on Microarrays
General Lecture on Microarrays

Research Highlights: Highlights from the last year in nanomedicine
Research Highlights: Highlights from the last year in nanomedicine

< 1 ... 157 158 159 160 161 162 163 164 165 ... 222 >

Comparative genomic hybridization



Comparative genomic hybridization is a molecular cytogenetic method for analysing copy number variations (CNVs) relative to ploidy level in the DNA of a test sample compared to a reference sample, without the need for culturing cells. The aim of this technique is to quickly and efficiently compare two genomic DNA samples arising from two sources, which are most often closely related, because it is suspected that they contain differences in terms of either gains or losses of either whole chromosomes or subchromosomal regions (a portion of a whole chromosome). This technique was originally developed for the evaluation of the differences between the chromosomal complements of solid tumor and normal tissue, and has an improved resoIution of 5-10 megabases compared to the more traditional cytogenetic analysis techniques of giemsa banding and fluorescence in situ hybridization (FISH) which are limited by the resolution of the microscope utilized.This is achieved through the use of competitive fluorescence in situ hybridization. In short, this involves the isolation of DNA from the two sources to be compared, most commonly a test and reference source, independent labelling of each DNA sample with a different fluorophores (fluorescent molecules) of different colours (usually red and green), denaturation of the DNA so that it is single stranded, and the hybridization of the two resultant samples in a 1:1 ratio to a normal metaphase spread of chromosomes, to which the labelled DNA samples will bind at their locus of origin. Using a fluorescence microscope and computer software, the differentially coloured fluorescent signals are then compared along the length of each chromosome for identification of chromosomal differences between the two sources. A higher intensity of the test sample colour in a specific region of a chromosome indicates the gain of material of that region in the corresponding source sample, while a higher intensity of the reference sample colour indicates the loss of material in the test sample in that specific region. A neutral colour (yellow when the fluorophore labels are red and green) indicates no difference between the two samples in that location.CGH is only able to detect unbalanced chromosomal abnormalities. This is because balanced chromosomal abnormalities such as reciprocal translocations, inversions or ring chromosomes do not affect copy number, which is what is detected by CGH technologies. CGH does, however, allow for the exploration of all 46 human chromosomes in single test and the discovery of deletions and duplications, even on the microscopic scale which may lead to the identification of candidate genes to be further explored by other cytological techniques.Through the use of DNA microarrays in conjunction with CGH techniques, the more specific form of array CGH (aCGH) has been developed, allowing for a locus-by-locus measure of CNV with increased resolution as low as 100 kilobases. This improved technique allows for the aetiology of known and unknown conditions to be discovered.
  • studyres.com © 2026
  • DMCA
  • Privacy
  • Terms
  • Report