DNA Structure, Function and Replication 1
... By Dr. Ingrid Waldron, Department of Biology, University of Pennsylvania, © 2014 ; Teachers are encouraged to copy this Student Handout for classroom use. A Word file (which can be used to prepare a modified version if desired) and Teacher Preparation Notes with learning goals, instructional suggest ...
... By Dr. Ingrid Waldron, Department of Biology, University of Pennsylvania, © 2014 ; Teachers are encouraged to copy this Student Handout for classroom use. A Word file (which can be used to prepare a modified version if desired) and Teacher Preparation Notes with learning goals, instructional suggest ...
DNA extraction from cheek cells protocol I mailed to you
... By Dr. Ingrid Waldron, Department of Biology, University of Pennsylvania, © 2014 ; Teachers are encouraged to copy this Student Handout for classroom use. A Word file (which can be used to prepare a modified version if desired) and Teacher Preparation Notes with learning goals, instructional suggest ...
... By Dr. Ingrid Waldron, Department of Biology, University of Pennsylvania, © 2014 ; Teachers are encouraged to copy this Student Handout for classroom use. A Word file (which can be used to prepare a modified version if desired) and Teacher Preparation Notes with learning goals, instructional suggest ...
- Horizon Discovery
... preservation of tissue architecture and cell morphology by cross-linking biomolecules. If fixation is not carried out under optimal conditions a tissue specimen can be irreversibly damaged. Methods of fixation vary according to sample types. Longer fixation periods may cause a high degree of DNA fra ...
... preservation of tissue architecture and cell morphology by cross-linking biomolecules. If fixation is not carried out under optimal conditions a tissue specimen can be irreversibly damaged. Methods of fixation vary according to sample types. Longer fixation periods may cause a high degree of DNA fra ...
View ePoster - 2015 AGU Fall Meeting
... • Shared OTU’s between cDNA and DNA further confirmed that presence of active members • D. audaxviator found in both DNA results and cDNA nifH results - it is active in the subsurface fixing N2. • No sulphate-reducing bacteria (dsrAB gene) was detected, however, after a few attempts of PCR amplifica ...
... • Shared OTU’s between cDNA and DNA further confirmed that presence of active members • D. audaxviator found in both DNA results and cDNA nifH results - it is active in the subsurface fixing N2. • No sulphate-reducing bacteria (dsrAB gene) was detected, however, after a few attempts of PCR amplifica ...
for DNA and RNA
... • If using microcentrifuge tubes, screw-top lids are preferred. Please use Parafilm® to cover lid for added seal precaution and clearly label tubes with water-resistant tags to ensure ink is not removed. Place individual tubes into a 50 mL conical tube filled with packing material (such as paper to ...
... • If using microcentrifuge tubes, screw-top lids are preferred. Please use Parafilm® to cover lid for added seal precaution and clearly label tubes with water-resistant tags to ensure ink is not removed. Place individual tubes into a 50 mL conical tube filled with packing material (such as paper to ...
HCS604.03 Exercise 1 Dr. Jones Spring 2005 Recombinant DNA
... or blunt-ended configuration. The enzyme has also been shown to catalyze the joining of RNA to either a DNA or RNA strand in a duplex molecule, but will not join singlestranded nucleic acids. The optimum incubation temperature for T4 DNA ligase is 16º C and when very high efficiency is desired, like ...
... or blunt-ended configuration. The enzyme has also been shown to catalyze the joining of RNA to either a DNA or RNA strand in a duplex molecule, but will not join singlestranded nucleic acids. The optimum incubation temperature for T4 DNA ligase is 16º C and when very high efficiency is desired, like ...
الشريحة 1
... PCR has also made amplification of genes an alternate approach to traditional cloning experiments. It is currently being used in: genome projects, DNA mapping sequencing. DNA based forensics paternity determination of evolutionary relationships. ...
... PCR has also made amplification of genes an alternate approach to traditional cloning experiments. It is currently being used in: genome projects, DNA mapping sequencing. DNA based forensics paternity determination of evolutionary relationships. ...
12.11 Restriction fragment analysis is a powerful method that
... 12.19 Connection: Gene therapy may someday help treat a variety of diseases • Techniques for manipulating DNA have potential for treating disease by altering an afflicted individual’s genes ...
... 12.19 Connection: Gene therapy may someday help treat a variety of diseases • Techniques for manipulating DNA have potential for treating disease by altering an afflicted individual’s genes ...
Biogenetic Engineering & Manipulating Genes
... What does the acronym PCR stand for and what does this process do? 2) What does Gel electrophoresis allow us to do? 3) Give two applications of DNA profiling. 4) What are the advantages and disadvantages of genetic screening? 5) How is a cDNA library different from a genomic library? (p. 388390) 6) ...
... What does the acronym PCR stand for and what does this process do? 2) What does Gel electrophoresis allow us to do? 3) Give two applications of DNA profiling. 4) What are the advantages and disadvantages of genetic screening? 5) How is a cDNA library different from a genomic library? (p. 388390) 6) ...
Replication
... RNase H is also not required since the removal of last primer is performed by DNApol I via its 5’exonuclease activity). ALL prokaryotic (bacterial) DNAs are always circular. It is the case not only for bacterial genomic DNA but also for plasmids, of course. Eukaryotes By whatever reason in eukaryote ...
... RNase H is also not required since the removal of last primer is performed by DNApol I via its 5’exonuclease activity). ALL prokaryotic (bacterial) DNAs are always circular. It is the case not only for bacterial genomic DNA but also for plasmids, of course. Eukaryotes By whatever reason in eukaryote ...
Specimens - BioMed Central
... clone gets smaller (e.g. a difference of 1 is required when the clone value is 0 and 0.36 when the clone value is 1). This approach was motivated by the observation that high level amplifications may arise within regions of copy number loss and thus it is necessary to consider the relative magnitude ...
... clone gets smaller (e.g. a difference of 1 is required when the clone value is 0 and 0.36 when the clone value is 1). This approach was motivated by the observation that high level amplifications may arise within regions of copy number loss and thus it is necessary to consider the relative magnitude ...
SOP 105: Procedures for DNA gel electrophoresis.
... The ratio of the readings at 260 nm and 280 nm (A260/ A280) provides an estimate of DNA purity with respect to contaminants that absorb UV light, such as protein. The A260/ A280 ratio is influenced considerably by pH. Since water is not buffered, the pH and the resulting A260/ A280 ratio can vary gr ...
... The ratio of the readings at 260 nm and 280 nm (A260/ A280) provides an estimate of DNA purity with respect to contaminants that absorb UV light, such as protein. The A260/ A280 ratio is influenced considerably by pH. Since water is not buffered, the pH and the resulting A260/ A280 ratio can vary gr ...
DNA Fingerprinting
... around the world. By comparing different DNA sequences, we can analyse the relationship between any two individuals. ...
... around the world. By comparing different DNA sequences, we can analyse the relationship between any two individuals. ...
Supplemental Materials and Methods (doc 44K)
... diameter) which develop through soil mixing as a result of frost action, are located on a peat plateau containing thick, unturbated peat soil. Peat circles lack vascular plant cover and show only sporadic growth of mosses and lichens (Repo et al., 2009). The vegetation of the peat plateau consists m ...
... diameter) which develop through soil mixing as a result of frost action, are located on a peat plateau containing thick, unturbated peat soil. Peat circles lack vascular plant cover and show only sporadic growth of mosses and lichens (Repo et al., 2009). The vegetation of the peat plateau consists m ...
Restriction Fragment Length Polymorphisms
... different DNA sequences and therefore produce different fragments when a particular restriction enzyme is used to cut an equivalent section of DNA. •The fragments produced are called RFLPs, ...
... different DNA sequences and therefore produce different fragments when a particular restriction enzyme is used to cut an equivalent section of DNA. •The fragments produced are called RFLPs, ...
Nucleic Acids - Structure and Replication
... For each section, the student counted the number of cells in mitosis and the total number of cells in each field of view. She then calculated a mitotic index for each section using the equation: ...
... For each section, the student counted the number of cells in mitosis and the total number of cells in each field of view. She then calculated a mitotic index for each section using the equation: ...
PTC Lab Classroom Slides
... Complete the miniPCR PTC Taster lab in two 45-min class periods, or in a single 3-h instruction block ...
... Complete the miniPCR PTC Taster lab in two 45-min class periods, or in a single 3-h instruction block ...
Gene Technology
... Cut with restriction enzymes into either small large or Bacterial artificial fragments fragments chromosome (BAC) ...
... Cut with restriction enzymes into either small large or Bacterial artificial fragments fragments chromosome (BAC) ...
File
... Base Excision Repair (BER) BER is a cellular mechanism that repairs damaged DNA throughout the cell cycle. It is primarily responsible for removing small, nonhelix distorting base lesions from the genome. BER is important for removing damaged bases that could otherwise cause mutations by mispairing ...
... Base Excision Repair (BER) BER is a cellular mechanism that repairs damaged DNA throughout the cell cycle. It is primarily responsible for removing small, nonhelix distorting base lesions from the genome. BER is important for removing damaged bases that could otherwise cause mutations by mispairing ...
Comparative genomic hybridization
Comparative genomic hybridization is a molecular cytogenetic method for analysing copy number variations (CNVs) relative to ploidy level in the DNA of a test sample compared to a reference sample, without the need for culturing cells. The aim of this technique is to quickly and efficiently compare two genomic DNA samples arising from two sources, which are most often closely related, because it is suspected that they contain differences in terms of either gains or losses of either whole chromosomes or subchromosomal regions (a portion of a whole chromosome). This technique was originally developed for the evaluation of the differences between the chromosomal complements of solid tumor and normal tissue, and has an improved resoIution of 5-10 megabases compared to the more traditional cytogenetic analysis techniques of giemsa banding and fluorescence in situ hybridization (FISH) which are limited by the resolution of the microscope utilized.This is achieved through the use of competitive fluorescence in situ hybridization. In short, this involves the isolation of DNA from the two sources to be compared, most commonly a test and reference source, independent labelling of each DNA sample with a different fluorophores (fluorescent molecules) of different colours (usually red and green), denaturation of the DNA so that it is single stranded, and the hybridization of the two resultant samples in a 1:1 ratio to a normal metaphase spread of chromosomes, to which the labelled DNA samples will bind at their locus of origin. Using a fluorescence microscope and computer software, the differentially coloured fluorescent signals are then compared along the length of each chromosome for identification of chromosomal differences between the two sources. A higher intensity of the test sample colour in a specific region of a chromosome indicates the gain of material of that region in the corresponding source sample, while a higher intensity of the reference sample colour indicates the loss of material in the test sample in that specific region. A neutral colour (yellow when the fluorophore labels are red and green) indicates no difference between the two samples in that location.CGH is only able to detect unbalanced chromosomal abnormalities. This is because balanced chromosomal abnormalities such as reciprocal translocations, inversions or ring chromosomes do not affect copy number, which is what is detected by CGH technologies. CGH does, however, allow for the exploration of all 46 human chromosomes in single test and the discovery of deletions and duplications, even on the microscopic scale which may lead to the identification of candidate genes to be further explored by other cytological techniques.Through the use of DNA microarrays in conjunction with CGH techniques, the more specific form of array CGH (aCGH) has been developed, allowing for a locus-by-locus measure of CNV with increased resolution as low as 100 kilobases. This improved technique allows for the aetiology of known and unknown conditions to be discovered.