Measuring forces in the DNA molecule
... However, Dietz also intends to apply the data directly to his current research: He uses DNA as programmable building material to construct machines on the order of nanometers. When doing so, he draws inspiration from the complex structures which can e.g. be found in cells and, among other things, se ...
... However, Dietz also intends to apply the data directly to his current research: He uses DNA as programmable building material to construct machines on the order of nanometers. When doing so, he draws inspiration from the complex structures which can e.g. be found in cells and, among other things, se ...
Biotechnology
... • Sequences called VNTRs (variable number of tandem repeats) are easily detectable if they are between two restriction enzyme recognition sites. • Different individuals have different numbers of repeats. Each gets two sequences of repeats, one from the mother and one from the father. • Using PCR and ...
... • Sequences called VNTRs (variable number of tandem repeats) are easily detectable if they are between two restriction enzyme recognition sites. • Different individuals have different numbers of repeats. Each gets two sequences of repeats, one from the mother and one from the father. • Using PCR and ...
DNA Double Helix KEY
... often called the "control center" because it controls all the activities of the cell including cell reproduction, and heredity. Chromosomes are microscopic, threadlike strands composed of the chemical DNA (short for deoxyribonucleic acid). In simple terms, DNA controls the production of proteins w ...
... often called the "control center" because it controls all the activities of the cell including cell reproduction, and heredity. Chromosomes are microscopic, threadlike strands composed of the chemical DNA (short for deoxyribonucleic acid). In simple terms, DNA controls the production of proteins w ...
DNA - The Double Helix
... often called the "control center" because it controls all the activities of the cell including cell reproduction, and heredity. Chromosomes are microscopic, threadlike strands composed of the chemical DNA (short for deoxyribonucleic acid). In simple terms, DNA controls the production of proteins wit ...
... often called the "control center" because it controls all the activities of the cell including cell reproduction, and heredity. Chromosomes are microscopic, threadlike strands composed of the chemical DNA (short for deoxyribonucleic acid). In simple terms, DNA controls the production of proteins wit ...
Stress protein synthesis: EMF interaction with DNA
... Activation of the stress response by both (non-thermal) power frequency and (thermal) radiofrequency EMF shows that the biological response is not simply related to the EMF energy. Protective biological responses occur at EMF thresholds that are far below thermal levels. Protein synthesis requir ...
... Activation of the stress response by both (non-thermal) power frequency and (thermal) radiofrequency EMF shows that the biological response is not simply related to the EMF energy. Protective biological responses occur at EMF thresholds that are far below thermal levels. Protein synthesis requir ...
Chapter 12 Primary Structure of Nucleic Acids Sequencing Strategies
... • For example, if the DNA sequence were: ATCCGGTAGCAATCGA ...
... • For example, if the DNA sequence were: ATCCGGTAGCAATCGA ...
11.2 Meiosis
... B. Metaphase I 1. Chromosomes moved to equator by spindle fibers. 2. Two chromatids remain together 3. Independent assortment a. Random distribution of homologous chromosomes ...
... B. Metaphase I 1. Chromosomes moved to equator by spindle fibers. 2. Two chromatids remain together 3. Independent assortment a. Random distribution of homologous chromosomes ...
Introduction to some basic features of genetic information
... linker region. In transcriptionally inactive chromatin there is a further order of packaging to form a structure known as the solenoid, comprising nucleosomes wrapped around a multimeric rod of H1 subunits. The solenoid is 30 nm in diameter and each turn contains six nucleosomes and six H1 molecules ...
... linker region. In transcriptionally inactive chromatin there is a further order of packaging to form a structure known as the solenoid, comprising nucleosomes wrapped around a multimeric rod of H1 subunits. The solenoid is 30 nm in diameter and each turn contains six nucleosomes and six H1 molecules ...
DNA Replication and DNA Repair Study Guide Focus on the
... DNA Replication and DNA Repair Study Guide Focus on the following. 1. Meselon and Stahl experiments showing semiconservatism. a. N-15 labeled DNA is heavier than N-14 DNA. b. N-15 bacteria placed in medium (which only contains N-14 DNA). c. CsCl density gradient used to note difference between N-15 ...
... DNA Replication and DNA Repair Study Guide Focus on the following. 1. Meselon and Stahl experiments showing semiconservatism. a. N-15 labeled DNA is heavier than N-14 DNA. b. N-15 bacteria placed in medium (which only contains N-14 DNA). c. CsCl density gradient used to note difference between N-15 ...
Identification of Copy Number Variants using genome graphs.
... While inversions do not cause any changes in copy number, an area that is deleted (SV) will correspond to a loss (CNV). Similarly, a region containing a tandem duplication will be annotated as both having an insertion (SV) and as exhibiting a gain (CNV). In this way, any PEM method for SV detection ...
... While inversions do not cause any changes in copy number, an area that is deleted (SV) will correspond to a loss (CNV). Similarly, a region containing a tandem duplication will be annotated as both having an insertion (SV) and as exhibiting a gain (CNV). In this way, any PEM method for SV detection ...
Protocol for MasterPure™ Gram Positive DNA
... needed to purify DNA from gram positive bacteria. These bacteria lyse more readily after treatment with Ready-Lyse™ Lysozyme and the Gram Positive Cell Lysis Solution. Ready-Lyse Lysozyme is a stable solution of a non-mammalian, non-avian recombinant lysozyme, with high specific activity and no net ...
... needed to purify DNA from gram positive bacteria. These bacteria lyse more readily after treatment with Ready-Lyse™ Lysozyme and the Gram Positive Cell Lysis Solution. Ready-Lyse Lysozyme is a stable solution of a non-mammalian, non-avian recombinant lysozyme, with high specific activity and no net ...
HotStart DNA Polymerase
... chemically modified to remain inactive until time, temperature and pH conditions are optimal. This results in higher specificity and greater yields when compared to standard DNA polymerases. o ...
... chemically modified to remain inactive until time, temperature and pH conditions are optimal. This results in higher specificity and greater yields when compared to standard DNA polymerases. o ...
APBiology 12
... Gene cloning is useful for two basic purposes: to make many copies of a particular gene and to create a protein product. o Isolated copies of a cloned gene may enable scientists to determine the gene’s nucleotide sequence or provide an organism with a new metabolic capability, such as pest resistanc ...
... Gene cloning is useful for two basic purposes: to make many copies of a particular gene and to create a protein product. o Isolated copies of a cloned gene may enable scientists to determine the gene’s nucleotide sequence or provide an organism with a new metabolic capability, such as pest resistanc ...
Perspectives on the Medical and Genetic Aspects
... Dr. Carey began by saying questions were welcomed. He also noted most participants were familiar with the basics of trisomy, so he would emphasize new information. The result was he completed about 10% of his forty prepared slides. The order of information that follows reflects the questions that in ...
... Dr. Carey began by saying questions were welcomed. He also noted most participants were familiar with the basics of trisomy, so he would emphasize new information. The result was he completed about 10% of his forty prepared slides. The order of information that follows reflects the questions that in ...
Total genomic DNA of non-treated and DHPA
... respectively. GMC GT500-L DNA standard (Genomac; orange peaks) was added to fluorescently labeled products of selective amplification (green peaks) and after denaturation, ss DNA fragments were separated using ABI Prism 3100 Genetic Analyzer. Obtained data were aligned and visualized using GeneMarke ...
... respectively. GMC GT500-L DNA standard (Genomac; orange peaks) was added to fluorescently labeled products of selective amplification (green peaks) and after denaturation, ss DNA fragments were separated using ABI Prism 3100 Genetic Analyzer. Obtained data were aligned and visualized using GeneMarke ...
Comparative genomic hybridization
Comparative genomic hybridization is a molecular cytogenetic method for analysing copy number variations (CNVs) relative to ploidy level in the DNA of a test sample compared to a reference sample, without the need for culturing cells. The aim of this technique is to quickly and efficiently compare two genomic DNA samples arising from two sources, which are most often closely related, because it is suspected that they contain differences in terms of either gains or losses of either whole chromosomes or subchromosomal regions (a portion of a whole chromosome). This technique was originally developed for the evaluation of the differences between the chromosomal complements of solid tumor and normal tissue, and has an improved resoIution of 5-10 megabases compared to the more traditional cytogenetic analysis techniques of giemsa banding and fluorescence in situ hybridization (FISH) which are limited by the resolution of the microscope utilized.This is achieved through the use of competitive fluorescence in situ hybridization. In short, this involves the isolation of DNA from the two sources to be compared, most commonly a test and reference source, independent labelling of each DNA sample with a different fluorophores (fluorescent molecules) of different colours (usually red and green), denaturation of the DNA so that it is single stranded, and the hybridization of the two resultant samples in a 1:1 ratio to a normal metaphase spread of chromosomes, to which the labelled DNA samples will bind at their locus of origin. Using a fluorescence microscope and computer software, the differentially coloured fluorescent signals are then compared along the length of each chromosome for identification of chromosomal differences between the two sources. A higher intensity of the test sample colour in a specific region of a chromosome indicates the gain of material of that region in the corresponding source sample, while a higher intensity of the reference sample colour indicates the loss of material in the test sample in that specific region. A neutral colour (yellow when the fluorophore labels are red and green) indicates no difference between the two samples in that location.CGH is only able to detect unbalanced chromosomal abnormalities. This is because balanced chromosomal abnormalities such as reciprocal translocations, inversions or ring chromosomes do not affect copy number, which is what is detected by CGH technologies. CGH does, however, allow for the exploration of all 46 human chromosomes in single test and the discovery of deletions and duplications, even on the microscopic scale which may lead to the identification of candidate genes to be further explored by other cytological techniques.Through the use of DNA microarrays in conjunction with CGH techniques, the more specific form of array CGH (aCGH) has been developed, allowing for a locus-by-locus measure of CNV with increased resolution as low as 100 kilobases. This improved technique allows for the aetiology of known and unknown conditions to be discovered.