Total genomic DNA of non-treated and DHPA
... respectively. GMC GT500-L DNA standard (Genomac; orange peaks) was added to fluorescently labeled products of selective amplification (green peaks) and after denaturation, ss DNA fragments were separated using ABI Prism 3100 Genetic Analyzer. Obtained data were aligned and visualized using GeneMarke ...
... respectively. GMC GT500-L DNA standard (Genomac; orange peaks) was added to fluorescently labeled products of selective amplification (green peaks) and after denaturation, ss DNA fragments were separated using ABI Prism 3100 Genetic Analyzer. Obtained data were aligned and visualized using GeneMarke ...
Restriction Enzymes
... organism from which they are isolated. • This is done by using the first letter of the genus followed by the first two letters of the species and additional letter or number represent the strain or serotypes. ...
... organism from which they are isolated. • This is done by using the first letter of the genus followed by the first two letters of the species and additional letter or number represent the strain or serotypes. ...
Single Nucleotide Polymorphism Microarray testing
... Each bead has thousands of ‘oligonucleotide’ probes attached, corresponding to a specific region of each chromosome.Reactions then take place on this slide, involving fluorescently labelled reagents. Regions where the genomic copy number differs between the patient’s DNA and a reference sample set c ...
... Each bead has thousands of ‘oligonucleotide’ probes attached, corresponding to a specific region of each chromosome.Reactions then take place on this slide, involving fluorescently labelled reagents. Regions where the genomic copy number differs between the patient’s DNA and a reference sample set c ...
DNA Replication - susanpittinaro
... Transformation = change in phenotype something in heat-killed bacteria could still transmit disease-causing properties ...
... Transformation = change in phenotype something in heat-killed bacteria could still transmit disease-causing properties ...
Document
... buffer (salt, pH) for enzyme to work. Mimics cellular conditions of bacteria they come from. ...
... buffer (salt, pH) for enzyme to work. Mimics cellular conditions of bacteria they come from. ...
1) - life.illinois.edu
... i). (5 Points). Which mechanism of transposition does this experiment support? Why? (Use a diagram or precise language to explain your answer). The result supports replicative transposition because a transposition event will transfer an element containing a single strand of Tn88-lacZ which is replic ...
... i). (5 Points). Which mechanism of transposition does this experiment support? Why? (Use a diagram or precise language to explain your answer). The result supports replicative transposition because a transposition event will transfer an element containing a single strand of Tn88-lacZ which is replic ...
I-Modified Nucleosides as DNA-Sugar Centered Radical Precursors
... interaction of DNA, oligonucleotides or nucleosides with ionizing radiation (γ-radiolysis), metal complexes (Mn-TMPyP, Cu(oP)2 and anticancer drugs such as enediynes). Photolysis of photoreactive groups that are specific C1’ radical precursors have been used by Greenberg and Chatgilialoglu for kinet ...
... interaction of DNA, oligonucleotides or nucleosides with ionizing radiation (γ-radiolysis), metal complexes (Mn-TMPyP, Cu(oP)2 and anticancer drugs such as enediynes). Photolysis of photoreactive groups that are specific C1’ radical precursors have been used by Greenberg and Chatgilialoglu for kinet ...
幻灯片 1
... ligation cycles join oligonucleotides into increasingly larger strands. A final PCR step is often employed to amplify the full-length target from the incomplete products. ...
... ligation cycles join oligonucleotides into increasingly larger strands. A final PCR step is often employed to amplify the full-length target from the incomplete products. ...
Genome-wide analysis by SNP Array
... CNVs can cause congenital diseases involving microduplications or microdeletions. All of the current scientific data reinforces the idea that the application of karyotyping or FISH remains insufficient for the diagnosis of the micro-rearrangements involved in ID and CA. Low karyotyping resolution ( ...
... CNVs can cause congenital diseases involving microduplications or microdeletions. All of the current scientific data reinforces the idea that the application of karyotyping or FISH remains insufficient for the diagnosis of the micro-rearrangements involved in ID and CA. Low karyotyping resolution ( ...
Document
... of a single gene A human gene can contain 40,000 1,000,000 base pairs. • That represents only about 0.03% of the entire genome. • We have the goal of identifying all human genes - Human Genome Project. • The first step is to construct a genomic library - brute force, hit or miss approach. ...
... of a single gene A human gene can contain 40,000 1,000,000 base pairs. • That represents only about 0.03% of the entire genome. • We have the goal of identifying all human genes - Human Genome Project. • The first step is to construct a genomic library - brute force, hit or miss approach. ...
Polymerase Chain Reaction (PCR)
... father. The genes are amplified using PCR, and then run through electrophoresis. The position of the two bands on the electrophoresis gel depends on the exact number of repeats at the locus. ...
... father. The genes are amplified using PCR, and then run through electrophoresis. The position of the two bands on the electrophoresis gel depends on the exact number of repeats at the locus. ...
DNA
... Transgenic Organisms • Transgenic Bacteria: – When a gene coding for a human protein (like a hormone or enzyme) is inserted into bacteria, the new recombinant cells may produce LARGE amounts of the protein. – The human growth hormone, a hormone required for growth and development, was incredibly ra ...
... Transgenic Organisms • Transgenic Bacteria: – When a gene coding for a human protein (like a hormone or enzyme) is inserted into bacteria, the new recombinant cells may produce LARGE amounts of the protein. – The human growth hormone, a hormone required for growth and development, was incredibly ra ...
Microarray poster-final - London Regional Genomics Centre
... Microarrays are a modern high throughput technology for interrogating RNA or DNA. The probes are immobilized on the array surface and the fluorescently labeled target is hybridized to the array. Results from microarray experiments can provide insights into differential gene expression, or genotyping ...
... Microarrays are a modern high throughput technology for interrogating RNA or DNA. The probes are immobilized on the array surface and the fluorescently labeled target is hybridized to the array. Results from microarray experiments can provide insights into differential gene expression, or genotyping ...
4.04 Workfile
... When law enforcement is faced with a real crime and must analyze a crime scene, they take a much more detailed approach. Using science and technology, they can conduct what is called a forensic analysis. For example, the police might find written documents and have the handwriting analyzed by an exp ...
... When law enforcement is faced with a real crime and must analyze a crime scene, they take a much more detailed approach. Using science and technology, they can conduct what is called a forensic analysis. For example, the police might find written documents and have the handwriting analyzed by an exp ...
Designing Molecular Machines·
... going to be. It's a simple mathematical exercise, really. There are 64--4 x 4 x 4, or 4 3-possible three-letter words we can make with a four-letter alphabet. In the three billion letters of the genome, each one of those 64 words should appear about 16 million times, assuming that all four letters, ...
... going to be. It's a simple mathematical exercise, really. There are 64--4 x 4 x 4, or 4 3-possible three-letter words we can make with a four-letter alphabet. In the three billion letters of the genome, each one of those 64 words should appear about 16 million times, assuming that all four letters, ...
Amniocentesis and CVS: QF-PCR analysis. Information for Parents
... ▪ QF-PCR can count the chromosomes mentioned above very accurately and rapidly. What are the limitations of QF-PCR? ▪ QF-PCR does not count or visualise any other chromosomes apart from 21, 18, 13, X and Y, so it will not detect other chromosome abnormalities or alterations in single genes. Many gen ...
... ▪ QF-PCR can count the chromosomes mentioned above very accurately and rapidly. What are the limitations of QF-PCR? ▪ QF-PCR does not count or visualise any other chromosomes apart from 21, 18, 13, X and Y, so it will not detect other chromosome abnormalities or alterations in single genes. Many gen ...
Molecular Typing Of microorganisms
... Sequencing of a single locus may not be reliable result ...
... Sequencing of a single locus may not be reliable result ...
Unit review questions
... 1. What scientist is responsible for our study of heredity? 2. Define heredity. 3. What plant did Mendel use for his hereditary experiments? 4. Name the 7 characteristics, giving both dominant and recessive forms of the pea plants, in Mendel’s experiments. 5. In order to study pea plant traits, Mend ...
... 1. What scientist is responsible for our study of heredity? 2. Define heredity. 3. What plant did Mendel use for his hereditary experiments? 4. Name the 7 characteristics, giving both dominant and recessive forms of the pea plants, in Mendel’s experiments. 5. In order to study pea plant traits, Mend ...
Comparative genomic hybridization
Comparative genomic hybridization is a molecular cytogenetic method for analysing copy number variations (CNVs) relative to ploidy level in the DNA of a test sample compared to a reference sample, without the need for culturing cells. The aim of this technique is to quickly and efficiently compare two genomic DNA samples arising from two sources, which are most often closely related, because it is suspected that they contain differences in terms of either gains or losses of either whole chromosomes or subchromosomal regions (a portion of a whole chromosome). This technique was originally developed for the evaluation of the differences between the chromosomal complements of solid tumor and normal tissue, and has an improved resoIution of 5-10 megabases compared to the more traditional cytogenetic analysis techniques of giemsa banding and fluorescence in situ hybridization (FISH) which are limited by the resolution of the microscope utilized.This is achieved through the use of competitive fluorescence in situ hybridization. In short, this involves the isolation of DNA from the two sources to be compared, most commonly a test and reference source, independent labelling of each DNA sample with a different fluorophores (fluorescent molecules) of different colours (usually red and green), denaturation of the DNA so that it is single stranded, and the hybridization of the two resultant samples in a 1:1 ratio to a normal metaphase spread of chromosomes, to which the labelled DNA samples will bind at their locus of origin. Using a fluorescence microscope and computer software, the differentially coloured fluorescent signals are then compared along the length of each chromosome for identification of chromosomal differences between the two sources. A higher intensity of the test sample colour in a specific region of a chromosome indicates the gain of material of that region in the corresponding source sample, while a higher intensity of the reference sample colour indicates the loss of material in the test sample in that specific region. A neutral colour (yellow when the fluorophore labels are red and green) indicates no difference between the two samples in that location.CGH is only able to detect unbalanced chromosomal abnormalities. This is because balanced chromosomal abnormalities such as reciprocal translocations, inversions or ring chromosomes do not affect copy number, which is what is detected by CGH technologies. CGH does, however, allow for the exploration of all 46 human chromosomes in single test and the discovery of deletions and duplications, even on the microscopic scale which may lead to the identification of candidate genes to be further explored by other cytological techniques.Through the use of DNA microarrays in conjunction with CGH techniques, the more specific form of array CGH (aCGH) has been developed, allowing for a locus-by-locus measure of CNV with increased resolution as low as 100 kilobases. This improved technique allows for the aetiology of known and unknown conditions to be discovered.