Amyloid Deposits: Protection Against Toxic Protein
... Prion diseases or spongiform encephalopathies can present in numerous ways, such as sporadic versus variant Creutzfeldt-Jakob disease (CJD). While the prion disease subtypes all involve the accumulation of a proteinase-K resistant form of the prion protein PrP (PrPres), they each affect different br ...
... Prion diseases or spongiform encephalopathies can present in numerous ways, such as sporadic versus variant Creutzfeldt-Jakob disease (CJD). While the prion disease subtypes all involve the accumulation of a proteinase-K resistant form of the prion protein PrP (PrPres), they each affect different br ...
Classification and substrate head-group specificity of membrane
... membrane-bound desaturases [25]. For a very diverse protein family, such as the membrane FADs, constructing a high-quality multiple sequence alignment can be challenging [30], which limits our ability to obtain evolutionary information from phylogenetic analysis and to annotate the possible function ...
... membrane-bound desaturases [25]. For a very diverse protein family, such as the membrane FADs, constructing a high-quality multiple sequence alignment can be challenging [30], which limits our ability to obtain evolutionary information from phylogenetic analysis and to annotate the possible function ...
proteins: three-dimensional structure
... than the 3.6 residues per turn of a standard a helix, the two keratin helices are inclined about 18° relative to one another, resulting in the coiled coil arrangement. This conformation allows the contacting side chains to interdigitate (Fig. 6-14b). The higher order structure of a keratin is not we ...
... than the 3.6 residues per turn of a standard a helix, the two keratin helices are inclined about 18° relative to one another, resulting in the coiled coil arrangement. This conformation allows the contacting side chains to interdigitate (Fig. 6-14b). The higher order structure of a keratin is not we ...
2015 department of medicine research day
... Tail amputation was performed three days post fertilization (dpf). Post-amputation fishes were treated with or without 25 µg/ml UFP in E3 solution. UFP induced abnormal vascular repair in the zebrafish model (n=16 out of 20) compared to control (n=4 out of 20). Abnormalities included incomplete repa ...
... Tail amputation was performed three days post fertilization (dpf). Post-amputation fishes were treated with or without 25 µg/ml UFP in E3 solution. UFP induced abnormal vascular repair in the zebrafish model (n=16 out of 20) compared to control (n=4 out of 20). Abnormalities included incomplete repa ...
ATP-binding-cassette (ABC) transport systems: Functional and
... by two short sequence motifs in their primary structure (`Walker' site A: GXXGXGKS/T, X can be varied; `Walker' site B: hhhhD, h stands for hydrophobic) that are supposed to constitute a nucleotide binding fold [7,8]. The Walker B-site is immediately preceded by a highly conserved sequence motif (`l ...
... by two short sequence motifs in their primary structure (`Walker' site A: GXXGXGKS/T, X can be varied; `Walker' site B: hhhhD, h stands for hydrophobic) that are supposed to constitute a nucleotide binding fold [7,8]. The Walker B-site is immediately preceded by a highly conserved sequence motif (`l ...
Separation of nuclear protein complexes by blue native
... whole cell lysates [8]. Moreover, an adaptation of the technique to agarose gel matrices with a higher separation limit was utilized for separation of very large protein complexes such as pyruvate dehydrogenase complex [4] or virus particles [9]. The procedure has several advantages that make the BN ...
... whole cell lysates [8]. Moreover, an adaptation of the technique to agarose gel matrices with a higher separation limit was utilized for separation of very large protein complexes such as pyruvate dehydrogenase complex [4] or virus particles [9]. The procedure has several advantages that make the BN ...
Lecture Protein Metabolism
... High affinity for NH3 - Concentrates NH3 in cells – Uses ATP Because of N recycling this reaction may not be that important ...
... High affinity for NH3 - Concentrates NH3 in cells – Uses ATP Because of N recycling this reaction may not be that important ...
Chapter 12 Synapses and Neurotransmitters
... – cyclic AMP can induce several alternative effects in the cell • causes the production of an internal chemical that binds to a ligand-regulated ion gate from inside of the membrane, opening the gate and depolarizing the cell • can activate preexisting cytoplasmic enzymes that lead do diverse metabo ...
... – cyclic AMP can induce several alternative effects in the cell • causes the production of an internal chemical that binds to a ligand-regulated ion gate from inside of the membrane, opening the gate and depolarizing the cell • can activate preexisting cytoplasmic enzymes that lead do diverse metabo ...
Protein Catabolism
... The biosynthesis of proteins requires a continuous source of amino acids. Amino acids also provide cells with a source of nitrogen for the synthesis of nitrogen containing biomolecules such as nucleotides. Amino acids are generated by the digestion of proteins in the intestine or by the degradation ...
... The biosynthesis of proteins requires a continuous source of amino acids. Amino acids also provide cells with a source of nitrogen for the synthesis of nitrogen containing biomolecules such as nucleotides. Amino acids are generated by the digestion of proteins in the intestine or by the degradation ...
Cysteine 230 Modulates Tumor Necrosis Factor
... have been identified as apoptosis inducers. On binding to cognate receptors, TNF-␣ and FasL recruit cellular components including Fas-associated death domain-containing protein (4 – 6) and procaspase-8 to signal apoptotic activity (7–9). This initial signaling event is followed by serial activation ...
... have been identified as apoptosis inducers. On binding to cognate receptors, TNF-␣ and FasL recruit cellular components including Fas-associated death domain-containing protein (4 – 6) and procaspase-8 to signal apoptotic activity (7–9). This initial signaling event is followed by serial activation ...
Circadian Rhythm of Intestinal SucraseActivity in Rats
... Clark (15). All assays were done in duplicate. Statistical evaluation of differences between means was done by t test. Determination of specific activity offree amino acids. Aliquots of individual mucosal homogenates containing 5 mg of protein were pooled for each time point after injection of ['4C] ...
... Clark (15). All assays were done in duplicate. Statistical evaluation of differences between means was done by t test. Determination of specific activity offree amino acids. Aliquots of individual mucosal homogenates containing 5 mg of protein were pooled for each time point after injection of ['4C] ...
Electrophoretic Extraction and Proteomic Characterization of
... Much of the ocean is influenced by nitrogen limitation [1], and thus, understanding marine protein cycling is important for tracking the global organic nitrogen cycle. Solid state NMR has provided evidence that the majority of organic nitrogen in dissolved and particulate marine organic matter conta ...
... Much of the ocean is influenced by nitrogen limitation [1], and thus, understanding marine protein cycling is important for tracking the global organic nitrogen cycle. Solid state NMR has provided evidence that the majority of organic nitrogen in dissolved and particulate marine organic matter conta ...
Biosensor - PharmaStreet
... synthetic sensor that mimics the function of a natural biosensor . These can include aptasensors , where apta -sensors use aptamers as the biocomponent. Aptamers are synthetic single stranded nucleic acid that can be designed to identify or recognize amino-acids, oligosaccharides , peptides , and pr ...
... synthetic sensor that mimics the function of a natural biosensor . These can include aptasensors , where apta -sensors use aptamers as the biocomponent. Aptamers are synthetic single stranded nucleic acid that can be designed to identify or recognize amino-acids, oligosaccharides , peptides , and pr ...
Interaction of Graphene Oxide with Proteins and
... Graphene oxide is a highly oxidized form of chemically modified graphene, produced by oxidation of crystalline graphite followed by sonication or other dispersion methods to produce monolayer material, typically in aqueous suspension. The structure of GO consists of single-atom-thick carbon sheets w ...
... Graphene oxide is a highly oxidized form of chemically modified graphene, produced by oxidation of crystalline graphite followed by sonication or other dispersion methods to produce monolayer material, typically in aqueous suspension. The structure of GO consists of single-atom-thick carbon sheets w ...
Thermodynamic analysis of the unfolding and stability of the dimeric
... to prevent DNA duplex melting [7] has also been described. HU proteins from several species of bacillus growing in environments of different temperatures have already been isolated and studied [4,7,15–18]. The close sequence homology among them suggests that their native structure must be very simil ...
... to prevent DNA duplex melting [7] has also been described. HU proteins from several species of bacillus growing in environments of different temperatures have already been isolated and studied [4,7,15–18]. The close sequence homology among them suggests that their native structure must be very simil ...
Receptor-mediated sorting of soluble vacuolar proteins: myths, facts
... VSRs are found in all land plants, as well as in several algae (chlorophytes, e.g. Chlamydomonas, Ostreococcus, and Micromonas) but not in rhodophytes or glaucophytes. A VSR is also present in the genome of the most simple eukaryotic organism, Ostreococcus tauri, a chlorophyte and the smallest known ...
... VSRs are found in all land plants, as well as in several algae (chlorophytes, e.g. Chlamydomonas, Ostreococcus, and Micromonas) but not in rhodophytes or glaucophytes. A VSR is also present in the genome of the most simple eukaryotic organism, Ostreococcus tauri, a chlorophyte and the smallest known ...
Structural Influences: Cholesterol, Drug, and Proton Binding to Full
... Isotopically labeled M2FL protein was expressed with N-terminal histidine-6 tag from the BL21 RP codon plus Escherichia coli. Initial cultures were grown in rich media. Two liters of cells were pelleted and washed with 50 mL of M9 minimal media before growing 1 L of M9 culture supplemented with a 10 ...
... Isotopically labeled M2FL protein was expressed with N-terminal histidine-6 tag from the BL21 RP codon plus Escherichia coli. Initial cultures were grown in rich media. Two liters of cells were pelleted and washed with 50 mL of M9 minimal media before growing 1 L of M9 culture supplemented with a 10 ...
Proteomic Analysis of Neisseria gonorrhoeae Biofilms
... gonococcal blebbing as piliated gonococcal msbB mutants that are defective in membrane blebbing fail to form biofilms [11]. In addition, blebs have been shown to act as a vehicle for the extracellular transport of gonococcal DNA [12]. Our studies would suggest this factor combined with death of orga ...
... gonococcal blebbing as piliated gonococcal msbB mutants that are defective in membrane blebbing fail to form biofilms [11]. In addition, blebs have been shown to act as a vehicle for the extracellular transport of gonococcal DNA [12]. Our studies would suggest this factor combined with death of orga ...
us whey proteins in ready-to-drink beverages
... isolates (containing 90-92% protein), or whey peptides, which provide distinct nutritional and functional advantages. In some cases, whey proteins are combined with other proteins such as vegetableor casein-based ingredients to create a particular overall amino acid composition or textural character ...
... isolates (containing 90-92% protein), or whey peptides, which provide distinct nutritional and functional advantages. In some cases, whey proteins are combined with other proteins such as vegetableor casein-based ingredients to create a particular overall amino acid composition or textural character ...
U.S. WHEY PROTEINS IN READY-TO-DRINK BEVERAGES
... isolates (containing 90-92% protein), or whey peptides, which provide distinct nutritional and functional advantages. In some cases, whey proteins are combined with other proteins such as vegetableor casein-based ingredients to create a particular overall amino acid composition or textural character ...
... isolates (containing 90-92% protein), or whey peptides, which provide distinct nutritional and functional advantages. In some cases, whey proteins are combined with other proteins such as vegetableor casein-based ingredients to create a particular overall amino acid composition or textural character ...
669 Salmonella typhimurium - Journal of General Virology
... in the medium until some time has passed, the extent of which depends on the multiplicity of tails added (Israel et al. ~972). The matter is perhaps best put in terms of probability. After each cleavage a tail protein could (i) continue down the chain; (ii) dissociate and immediately bind to the sam ...
... in the medium until some time has passed, the extent of which depends on the multiplicity of tails added (Israel et al. ~972). The matter is perhaps best put in terms of probability. After each cleavage a tail protein could (i) continue down the chain; (ii) dissociate and immediately bind to the sam ...
38
... sequence signatures of protein disorder are present. The popular choice of training set to construct these predictors often use reported missing residues in X-ray crystallographic structures, but reported temperature factors (B-factors) and NMR characterized disordered regions have also been used. F ...
... sequence signatures of protein disorder are present. The popular choice of training set to construct these predictors often use reported missing residues in X-ray crystallographic structures, but reported temperature factors (B-factors) and NMR characterized disordered regions have also been used. F ...
Probing the receptor recognition site of the FimH adhesin by f
... chosen to exploit the compatibility of type 1 fimbriae with another fimbrial system, namely F1C fimbriae. Unlike type 1 fimbriae, F1C fimbriae do not target to Dmannosides; in fact, they do not agglutinate any known red blood cells or yeasts. However, they are known to confer adhesion to an unknown ...
... chosen to exploit the compatibility of type 1 fimbriae with another fimbrial system, namely F1C fimbriae. Unlike type 1 fimbriae, F1C fimbriae do not target to Dmannosides; in fact, they do not agglutinate any known red blood cells or yeasts. However, they are known to confer adhesion to an unknown ...
Detection of plum pox potyviral protein–protein interactions in planta
... PPV_CP interaction studies suggested that the F1 and F2 CP fragments contain interacting protein domains and that C-terminal aa 222–315 were not involved in the selfinteraction of PPV_CP. This is in contrast to the results of Kang et al. (2006), who reported the C-terminal aa 171–285 of SMV-G7H_CP t ...
... PPV_CP interaction studies suggested that the F1 and F2 CP fragments contain interacting protein domains and that C-terminal aa 222–315 were not involved in the selfinteraction of PPV_CP. This is in contrast to the results of Kang et al. (2006), who reported the C-terminal aa 171–285 of SMV-G7H_CP t ...
G protein–coupled receptor
G protein–coupled receptors (GPCRs), also known as seven-transmembrane domain receptors, 7TM receptors, heptahelical receptors, serpentine receptor, and G protein–linked receptors (GPLR), constitute a large protein family of receptors that sense molecules outside the cell and activate inside signal transduction pathways and, ultimately, cellular responses. Coupling with G proteins, they are called seven-transmembrane receptors because they pass through the cell membrane seven times.G protein–coupled receptors are found only in eukaryotes, including yeast, choanoflagellates, and animals. The ligands that bind and activate these receptors include light-sensitive compounds, odors, pheromones, hormones, and neurotransmitters, and vary in size from small molecules to peptides to large proteins. G protein–coupled receptors are involved in many diseases, and are also the target of approximately 40% of all modern medicinal drugs. Two of the United States's top five selling drugs (Hydrocodone and Lisinopril) act by targeting a G protein–coupled receptor. The 2012 Nobel Prize in Chemistry was awarded to Brian Kobilka and Robert Lefkowitz for their work that was ""crucial for understanding how G protein–coupled receptors function."". There have been at least seven other Nobel Prizes awarded for some aspect of G protein–mediated signaling.There are two principal signal transduction pathways involving the G protein–coupled receptors: the cAMP signal pathway and the phosphatidylinositol signal pathway. When a ligand binds to the GPCR it causes a conformational change in the GPCR, which allows it to act as a guanine nucleotide exchange factor (GEF). The GPCR can then activate an associated G protein by exchanging its bound GDP for a GTP. The G protein's α subunit, together with the bound GTP, can then dissociate from the β and γ subunits to further affect intracellular signaling proteins or target functional proteins directly depending on the α subunit type (Gαs, Gαi/o, Gαq/11, Gα12/13).