Continued..
... which is found in a widely varying family of DNA-binding proteins. The conserved cysteine and histidine residues in this sequence motif form ligands to a zinc ion, which is essential to stabilize the tertiary structure. Conservation is sometimes of a class of residues rather than a specific resi ...
... which is found in a widely varying family of DNA-binding proteins. The conserved cysteine and histidine residues in this sequence motif form ligands to a zinc ion, which is essential to stabilize the tertiary structure. Conservation is sometimes of a class of residues rather than a specific resi ...
Biochemistry of Signal Transduction and Regulation - Beck-Shop
... The function of communicating with the environment is achieved through a number of pathways that receive and process signals originating from the external environment, from other cells within the organism and also from different regions within the cell. In addition to adapting the function of an org ...
... The function of communicating with the environment is achieved through a number of pathways that receive and process signals originating from the external environment, from other cells within the organism and also from different regions within the cell. In addition to adapting the function of an org ...
template
... Haptic devices have been used for ligand-protein docking problem [Subasi and Basdogan 2008; Nagata et al. 2001] to simulate electrostatic potential energy that plays an important role during the process. In haptic applications of ligand-protein docking problem, user moves the ligand with a haptic de ...
... Haptic devices have been used for ligand-protein docking problem [Subasi and Basdogan 2008; Nagata et al. 2001] to simulate electrostatic potential energy that plays an important role during the process. In haptic applications of ligand-protein docking problem, user moves the ligand with a haptic de ...
AMP-activated/SNF1 protein kinases: conserved guardians of
... know almost nothing about the properties of the enzyme from S. pombe, but this structure does define the core interactions between the three subunits, which are likely to be conserved between different eukaryotes. The α-subunits: catalytic subunits. Two α-subunit iso forms (α1/α2) are encoded by di ...
... know almost nothing about the properties of the enzyme from S. pombe, but this structure does define the core interactions between the three subunits, which are likely to be conserved between different eukaryotes. The α-subunits: catalytic subunits. Two α-subunit iso forms (α1/α2) are encoded by di ...
the Sec7 family of guanine-nucleotide- exchange factors
... inactive, abortive complex. A separate subclass of Sec7-domain proteins is involved in signal transduction and possess a domain that mediates membrane binding in response to extracellular signals. ...
... inactive, abortive complex. A separate subclass of Sec7-domain proteins is involved in signal transduction and possess a domain that mediates membrane binding in response to extracellular signals. ...
NUCLEAR PROTEIN KINASE ACTIVITIES DURING THE CELL
... been studied during the eukaryotic cell cycle. At the level of resolution provided by one-dimensional gel electrophoresis the complement of nonhistone chromosomal proteins does not vary [14--16]. However, cell cycle stage-specific variations have been observed in the rates of synthesis, turnover and ...
... been studied during the eukaryotic cell cycle. At the level of resolution provided by one-dimensional gel electrophoresis the complement of nonhistone chromosomal proteins does not vary [14--16]. However, cell cycle stage-specific variations have been observed in the rates of synthesis, turnover and ...
BMC Bioinformatics
... revealed some statistical biases in the N-terminal region instead of providing specific sequences responsible for the protein secretion. Moreover, many effectors do not fulfill these requirements. Some effectors even possess none of these features [8]. Petnicki-Ocwieja et al. [16] mentioned that alt ...
... revealed some statistical biases in the N-terminal region instead of providing specific sequences responsible for the protein secretion. Moreover, many effectors do not fulfill these requirements. Some effectors even possess none of these features [8]. Petnicki-Ocwieja et al. [16] mentioned that alt ...
The protein acetylome and the regulation of metabolism - Serval
... Mak10 and Mak31. Homologs to all these subunits are present in plants, and complementation of yeast natc mutants with the homologous Arabidopsis (Arabidopsis thaliana) genes has been reported [20]. NatA mainly recognizes N-terminal single amino acids, such as Ser, Ala, Thr or Gly. NatB specifically ...
... Mak10 and Mak31. Homologs to all these subunits are present in plants, and complementation of yeast natc mutants with the homologous Arabidopsis (Arabidopsis thaliana) genes has been reported [20]. NatA mainly recognizes N-terminal single amino acids, such as Ser, Ala, Thr or Gly. NatB specifically ...
Biogenesis of hepatitis C virus envelope glycoproteins
... participate in the assembly of the infectious particle and also play a crucial role in virus entry by binding to a receptor present on the host cell and inducing fusion between the viral envelope and a membrane of the host cell. To fulfil these functions, viral envelope proteins have to adopt dramat ...
... participate in the assembly of the infectious particle and also play a crucial role in virus entry by binding to a receptor present on the host cell and inducing fusion between the viral envelope and a membrane of the host cell. To fulfil these functions, viral envelope proteins have to adopt dramat ...
The proteome of the infectious bronchitis virus Beau
... Venn diagram (Fig. 3b). Similar to observations made when comparing uninfected and mock-infected allantoic fluids, the protein classes identified for the three preparations of IBV were largely the same. However, specific proteins within each protein class varied among the three preparations, resulti ...
... Venn diagram (Fig. 3b). Similar to observations made when comparing uninfected and mock-infected allantoic fluids, the protein classes identified for the three preparations of IBV were largely the same. However, specific proteins within each protein class varied among the three preparations, resulti ...
Short transmembrane domains with high
... To examine whether the geometric features of TMDs also act as determinants of localization in mammalian cells, CHO-K1 cells were transfected with mCherry-tagged Sso1 or fusions of the Sso1 cytoplasmic domain to the TMDs described in Fig. 2 and their subcellular localization was established by coloca ...
... To examine whether the geometric features of TMDs also act as determinants of localization in mammalian cells, CHO-K1 cells were transfected with mCherry-tagged Sso1 or fusions of the Sso1 cytoplasmic domain to the TMDs described in Fig. 2 and their subcellular localization was established by coloca ...
TFE3 contains two activation domains, one acidic and the other
... TFE3L activity was achieved with substoichiometric amounts of TFE3S expression plasmid. The correlation between the plasmid ratios at which TFE3S inhibited TFE3L activity and the ratio of these mRNA isoforms in vivo suggested that the dominant negative activity of TFE3S is biologically important. Se ...
... TFE3L activity was achieved with substoichiometric amounts of TFE3S expression plasmid. The correlation between the plasmid ratios at which TFE3S inhibited TFE3L activity and the ratio of these mRNA isoforms in vivo suggested that the dominant negative activity of TFE3S is biologically important. Se ...
Document
... the protein exceeds that which can be handled on a peptide synthesizer (∼ so the protein has to be expressed in E. coli. Second and more importantly, the amide I frequency is downshifted into a spectral range were many amino acid side chains absorb as well, in particular those containing carboxylate ...
... the protein exceeds that which can be handled on a peptide synthesizer (∼ so the protein has to be expressed in E. coli. Second and more importantly, the amide I frequency is downshifted into a spectral range were many amino acid side chains absorb as well, in particular those containing carboxylate ...
SDS-PAGE strongly overestimates the molecular
... to the carboxyterminally situated non+-helical domains (tailpieces) known to have an unusual amino acid composition [3] and in the case of H an extremely high content of serine phosphate [2,12]. In addition these domains are rather acidic [2,3]. There are several acidic proteins which, probably due ...
... to the carboxyterminally situated non+-helical domains (tailpieces) known to have an unusual amino acid composition [3] and in the case of H an extremely high content of serine phosphate [2,12]. In addition these domains are rather acidic [2,3]. There are several acidic proteins which, probably due ...
RbcL | Rubisco large subunit, form I and form II (50 µl) product
... AS03 037-100 | anti-RbcL, Rubisco large subunit, form I and form II (100 µl) AS03 037-200 | anti-RbcL, Rubisco large subunit, form I and form II, larger pack size (200 µl) of AS03 037, rabbit antibody AS03 037-HRP | anti-RbcL, Rubisco large subunit, form I and form II. HRP conjugated AS03 037PRE | R ...
... AS03 037-100 | anti-RbcL, Rubisco large subunit, form I and form II (100 µl) AS03 037-200 | anti-RbcL, Rubisco large subunit, form I and form II, larger pack size (200 µl) of AS03 037, rabbit antibody AS03 037-HRP | anti-RbcL, Rubisco large subunit, form I and form II. HRP conjugated AS03 037PRE | R ...
Tyrosine cross-linking of extracellular matrix is catalyzed by Duox, a
... Domain organization and sequence comparisons among gp91phox, h-Duox1, Ce-Duox1, and Ce-Duox2 The domain structure and transmembrane regions in gp91phox, h-Duox1/2, Ce-Duox1, and Ce-Duox2 are diagrammed in Fig. 1. Duox enzymes are homologous to gp91phox in their COOH termini (see http://www.biochem. ...
... Domain organization and sequence comparisons among gp91phox, h-Duox1, Ce-Duox1, and Ce-Duox2 The domain structure and transmembrane regions in gp91phox, h-Duox1/2, Ce-Duox1, and Ce-Duox2 are diagrammed in Fig. 1. Duox enzymes are homologous to gp91phox in their COOH termini (see http://www.biochem. ...
Modification of the signal sequence cleavage site of
... protein to the secretion pathway. This signal peptide is needed to target the protein and to initiate translocation across the membrane, in both Gram-negative and Grampositive bacteria (von Heijne, 1990; Pugsley, 1993; van Wely et al., 2001; Antelmann et al., 2001; and references therein). Upon tran ...
... protein to the secretion pathway. This signal peptide is needed to target the protein and to initiate translocation across the membrane, in both Gram-negative and Grampositive bacteria (von Heijne, 1990; Pugsley, 1993; van Wely et al., 2001; Antelmann et al., 2001; and references therein). Upon tran ...
Translation | Principles of Biology from Nature Education
... As translation occurs, the polypeptide folds spontaneously due to its primary sequence. Frequently, a chaperone protein called chaperonin also aids in proper folding. However, many proteins require additional modifications after translation to become fully functional. There are many types of post-tr ...
... As translation occurs, the polypeptide folds spontaneously due to its primary sequence. Frequently, a chaperone protein called chaperonin also aids in proper folding. However, many proteins require additional modifications after translation to become fully functional. There are many types of post-tr ...
Protein Kinase C–dependent Activation of Cytosolic
... buffer for SDS-PAGE and Western blotting or in the assay buffer supplemented with 5 mM DTT for the cPLA2 activity assay. Phosphorylation-induced mobility shift, SDS-PAGE, and Western blotting of MAP kinase. Cells cultured in a 6-well plate were washed four times with serum- and NaHCO3-free DME suppl ...
... buffer for SDS-PAGE and Western blotting or in the assay buffer supplemented with 5 mM DTT for the cPLA2 activity assay. Phosphorylation-induced mobility shift, SDS-PAGE, and Western blotting of MAP kinase. Cells cultured in a 6-well plate were washed four times with serum- and NaHCO3-free DME suppl ...
The syndapin protein family: linking membrane trafficking with the
... trafficking and organization of the actin cytoskeleton (Qualmann et al., 1999), a hypothesis that was followed up by more-detailed studies of the interactions with dynamin and NWASP. The relevance of the interaction with dynamin is strongly supported by coimmunoprecipitation studies of the endogenou ...
... trafficking and organization of the actin cytoskeleton (Qualmann et al., 1999), a hypothesis that was followed up by more-detailed studies of the interactions with dynamin and NWASP. The relevance of the interaction with dynamin is strongly supported by coimmunoprecipitation studies of the endogenou ...
Histoplasma capsulatum an effective mechanism for trans-cell wall transfer
... from H. capsulatum revealed the presence of bilayered, spherical vesicles (Fig. 1). Five hundred and eight vesicles were analysed and were found to range in size from 10 to 350 nm (Fig. 2). The electron density of the vesicles varied considerably, suggesting distinct contents ...
... from H. capsulatum revealed the presence of bilayered, spherical vesicles (Fig. 1). Five hundred and eight vesicles were analysed and were found to range in size from 10 to 350 nm (Fig. 2). The electron density of the vesicles varied considerably, suggesting distinct contents ...
chapter 11
... • A protein may have from about 50 to many thousands of amino acids, joined linearly by way of peptide bonds. • The information for determining the sequence of amino acids resides in the DNA of most cells. • A gene is the region of DNA responsible for the coding of a protein. • There are thousands o ...
... • A protein may have from about 50 to many thousands of amino acids, joined linearly by way of peptide bonds. • The information for determining the sequence of amino acids resides in the DNA of most cells. • A gene is the region of DNA responsible for the coding of a protein. • There are thousands o ...
Protein measurement with the Folin-phenol reagent
... If the protein is present in an already very dilute solution (less than 25 y per ml.), 0.5 ml. may be mixed with 0.5 ml. of an exactly double strength Reagent C and otherwise treated as above. Insoluble Proteins, etc.-Many protein precipitates, e.g. tungstate preHowever, cipitates, will dissolve rea ...
... If the protein is present in an already very dilute solution (less than 25 y per ml.), 0.5 ml. may be mixed with 0.5 ml. of an exactly double strength Reagent C and otherwise treated as above. Insoluble Proteins, etc.-Many protein precipitates, e.g. tungstate preHowever, cipitates, will dissolve rea ...
View PDF - Elly Nedivi
... gyrus. Other IEG mRNAs, such as c-fos, c-jun, and junB, were less consistently increased under the same experimental conditions, in line with earlier findings (63). While Cole et al. (48) found that stimulation of convergent inhibitory synaptic inputs known to block LTP also blocked IEG induction, W ...
... gyrus. Other IEG mRNAs, such as c-fos, c-jun, and junB, were less consistently increased under the same experimental conditions, in line with earlier findings (63). While Cole et al. (48) found that stimulation of convergent inhibitory synaptic inputs known to block LTP also blocked IEG induction, W ...
G protein–coupled receptor
G protein–coupled receptors (GPCRs), also known as seven-transmembrane domain receptors, 7TM receptors, heptahelical receptors, serpentine receptor, and G protein–linked receptors (GPLR), constitute a large protein family of receptors that sense molecules outside the cell and activate inside signal transduction pathways and, ultimately, cellular responses. Coupling with G proteins, they are called seven-transmembrane receptors because they pass through the cell membrane seven times.G protein–coupled receptors are found only in eukaryotes, including yeast, choanoflagellates, and animals. The ligands that bind and activate these receptors include light-sensitive compounds, odors, pheromones, hormones, and neurotransmitters, and vary in size from small molecules to peptides to large proteins. G protein–coupled receptors are involved in many diseases, and are also the target of approximately 40% of all modern medicinal drugs. Two of the United States's top five selling drugs (Hydrocodone and Lisinopril) act by targeting a G protein–coupled receptor. The 2012 Nobel Prize in Chemistry was awarded to Brian Kobilka and Robert Lefkowitz for their work that was ""crucial for understanding how G protein–coupled receptors function."". There have been at least seven other Nobel Prizes awarded for some aspect of G protein–mediated signaling.There are two principal signal transduction pathways involving the G protein–coupled receptors: the cAMP signal pathway and the phosphatidylinositol signal pathway. When a ligand binds to the GPCR it causes a conformational change in the GPCR, which allows it to act as a guanine nucleotide exchange factor (GEF). The GPCR can then activate an associated G protein by exchanging its bound GDP for a GTP. The G protein's α subunit, together with the bound GTP, can then dissociate from the β and γ subunits to further affect intracellular signaling proteins or target functional proteins directly depending on the α subunit type (Gαs, Gαi/o, Gαq/11, Gα12/13).