During Cytokinesis Light Chain, Tctex
... lymphocytes. These results strongly suggest that Fyn plays diverse cellular roles in addition to receptor-proximal signal transduction, and defining these alternative roles is an important challenge. Functional diversity between members of the Src family of kinases is mediated by four classical prot ...
... lymphocytes. These results strongly suggest that Fyn plays diverse cellular roles in addition to receptor-proximal signal transduction, and defining these alternative roles is an important challenge. Functional diversity between members of the Src family of kinases is mediated by four classical prot ...
endoplasmic reticulum stress and lipid metabolism
... consist of a core of mainly triglycerides and steryl esters, surrounded by a phospholipid monolayer and come into close approximation with the ER. The monolayer is coated by proteins that are involved ...
... consist of a core of mainly triglycerides and steryl esters, surrounded by a phospholipid monolayer and come into close approximation with the ER. The monolayer is coated by proteins that are involved ...
APPLICATION OF PANCREATIC ENZYMES IN HYDROLYSIS OF
... useful methods (crystallization, precipitation, membrane filtration, and anion exchange or affinity chromatography) for their isolation and purification have been developed [Hiidenhovi, 2007; Sokołowska et al., 2007; Trziszka et al., 2006]. Two of the by-products of these processes are ovoalbumin an ...
... useful methods (crystallization, precipitation, membrane filtration, and anion exchange or affinity chromatography) for their isolation and purification have been developed [Hiidenhovi, 2007; Sokołowska et al., 2007; Trziszka et al., 2006]. Two of the by-products of these processes are ovoalbumin an ...
Determination of Protein Molecular Weight
... three-dimensional shape is abolished, the protein no longer possesses biological activity. Proteins that have lost their specific folding patterns and biological activity but have their intact polypeptide chains are called denatured. Proteins which contain several polypeptide chains that are associa ...
... three-dimensional shape is abolished, the protein no longer possesses biological activity. Proteins that have lost their specific folding patterns and biological activity but have their intact polypeptide chains are called denatured. Proteins which contain several polypeptide chains that are associa ...
Characterisation of the Aspergillus niger dapB gene, which encodes
... of DapB resembles that of a type II membrane protein, with the N-terminus located in the cytosol, followed by a single membrane-spanning region, with the remaining portion located in the extracellular (lumen) compartment. Further sequence comparisons with the functional domains of other DPP IVs resu ...
... of DapB resembles that of a type II membrane protein, with the N-terminus located in the cytosol, followed by a single membrane-spanning region, with the remaining portion located in the extracellular (lumen) compartment. Further sequence comparisons with the functional domains of other DPP IVs resu ...
cytoplasm nucleus and the A specific subset of
... The SR proteins constitute a large family of nuclear phosphoproteins required for constitutive pre-mRNA splicing. These factors also have global, concentration-dependent effects on alternative splicing regulation and this activity is antagonized by members of the hnRNP A/B family of proteins. We sho ...
... The SR proteins constitute a large family of nuclear phosphoproteins required for constitutive pre-mRNA splicing. These factors also have global, concentration-dependent effects on alternative splicing regulation and this activity is antagonized by members of the hnRNP A/B family of proteins. We sho ...
selection of antigens for antibody-based proteomics
... number of alternatively spliced transcripts, and the proteins encoded by those splice variants can be modified after translation, the number of different proteins in the human proteome is huge. Adding the large group of proteins created by somatic rearrangement (e. g. immunoglobulins) and amino acid ...
... number of alternatively spliced transcripts, and the proteins encoded by those splice variants can be modified after translation, the number of different proteins in the human proteome is huge. Adding the large group of proteins created by somatic rearrangement (e. g. immunoglobulins) and amino acid ...
See Source - Pentelute Lab
... resolution LCMS (Figure 1b–d). We conducted additional sortagging studies to investigate two aspects of the oligoglycine αthioester: varying the C-terminal amino acid (Xaa) or varying the number of glycine residues. It has been demonstrated that the reactivity of a peptide αthioester is dependent up ...
... resolution LCMS (Figure 1b–d). We conducted additional sortagging studies to investigate two aspects of the oligoglycine αthioester: varying the C-terminal amino acid (Xaa) or varying the number of glycine residues. It has been demonstrated that the reactivity of a peptide αthioester is dependent up ...
34750 - Radboud Repository
... [4]. During the transformation of proto-mitochondrion to organelle, its proteome underwent a series of modifications, including, among others, the acquisition of a protein import machinery and an ADP/ATP carrier, leading to a situation in which only a minority of mitochondrial proteins can be traced ...
... [4]. During the transformation of proto-mitochondrion to organelle, its proteome underwent a series of modifications, including, among others, the acquisition of a protein import machinery and an ADP/ATP carrier, leading to a situation in which only a minority of mitochondrial proteins can be traced ...
UvA-DARE (Digital Academic Repository) Mitochondria in neutrophil
... their apoptosis. Most prominent amongst these proteins in neutrophils are Mcl-1 and Bfl-1. The main function of Mcl-1 in neutrophils seems to be inhibition of Bax translocation 5, but this thesis has demonstrated that the role of Bfl-1 in the apoptotic process is far from obvious. The function of Ba ...
... their apoptosis. Most prominent amongst these proteins in neutrophils are Mcl-1 and Bfl-1. The main function of Mcl-1 in neutrophils seems to be inhibition of Bax translocation 5, but this thesis has demonstrated that the role of Bfl-1 in the apoptotic process is far from obvious. The function of Ba ...
Synthetic human prion protein octapeptide repeat binds to the
... the clinical and laboratory practice for identification of the scrapie agent [6]. The NMR structure of the recombinant human prion protein, rhPrP (23–230), includes a globular domain (residues 125–228) and an N-terminal disordered tail [7]. The fragment between residues 53 and 85 consists of repeats ...
... the clinical and laboratory practice for identification of the scrapie agent [6]. The NMR structure of the recombinant human prion protein, rhPrP (23–230), includes a globular domain (residues 125–228) and an N-terminal disordered tail [7]. The fragment between residues 53 and 85 consists of repeats ...
Glycoprotein IIIa Is Phosphorylated in Intact Human
... 1). The purified material was essentially homogeneous (Fig 1, lane 1). Only one component of the complex was phosphorylated. This was identified as G P IIIa (lanes 2 and 4) on the basis of its co-electrophoresis with iodinated G P IIIa (lanes 3 and 5) from the purified G P IIb-IIIa complex. The poss ...
... 1). The purified material was essentially homogeneous (Fig 1, lane 1). Only one component of the complex was phosphorylated. This was identified as G P IIIa (lanes 2 and 4) on the basis of its co-electrophoresis with iodinated G P IIIa (lanes 3 and 5) from the purified G P IIb-IIIa complex. The poss ...
Protein S-nitrosylation in photosynthetic organisms: A
... It is well established that NO can form complexes with plant metalcontaining proteins and several studies have focused their attention on the interactions between NO and hemoglobin (Hb). In plants, there are three main types of Hb: symbiotic Hb, also named leghemoglobin (Lb), localized in nitrogen-fi ...
... It is well established that NO can form complexes with plant metalcontaining proteins and several studies have focused their attention on the interactions between NO and hemoglobin (Hb). In plants, there are three main types of Hb: symbiotic Hb, also named leghemoglobin (Lb), localized in nitrogen-fi ...
Nitrogen Balance and Protein Requirements: Definition and
... the Postprandial Phase The diurnal cycle of feeding and fasting is accompanied by concurrent changes in protein turnover. Protein feeding is necessary to replenish the body protein stores that would be wasted during fasting [21–24]. Because of this, nitrogen retention calculated on a daily basis is ...
... the Postprandial Phase The diurnal cycle of feeding and fasting is accompanied by concurrent changes in protein turnover. Protein feeding is necessary to replenish the body protein stores that would be wasted during fasting [21–24]. Because of this, nitrogen retention calculated on a daily basis is ...
Function and biotechnology of extremophilic enzymes in low water
... One of the underlying reasons for limited enzyme activity in extreme conditions is their effects on water structure and dynamics. When water activity is perturbed by extreme temperatures, high salinity, or other extreme conditions, normally structured liquid water may become limiting to enzymes, wit ...
... One of the underlying reasons for limited enzyme activity in extreme conditions is their effects on water structure and dynamics. When water activity is perturbed by extreme temperatures, high salinity, or other extreme conditions, normally structured liquid water may become limiting to enzymes, wit ...
Oxidized Amino Acids in Lens Protein with Age
... in thespectra, the more intense m/z = 865 and 871 ions were chosen assay for measurement of DT formed during radiolytic and for quantitation of DT andde-DT, respectively. Essentially identical MCO of the model proteins RNase and lysozyme (9). Howresults were obtained using the m/z = 805 and 811 ions ...
... in thespectra, the more intense m/z = 865 and 871 ions were chosen assay for measurement of DT formed during radiolytic and for quantitation of DT andde-DT, respectively. Essentially identical MCO of the model proteins RNase and lysozyme (9). Howresults were obtained using the m/z = 805 and 811 ions ...
Studying Protein Flexibility in a Statistical Framework: Tools and
... structural alignments on version 1.39 of the scop database, which represented most the known structures as of the beginning of 1999. From this they found 4403 pairs of domains that had appreciable sequence similarity yet had great structural differences; these represented putative instances of confo ...
... structural alignments on version 1.39 of the scop database, which represented most the known structures as of the beginning of 1999. From this they found 4403 pairs of domains that had appreciable sequence similarity yet had great structural differences; these represented putative instances of confo ...
Type and timing of protein feeding to optimize anabolism
... increase (even moderate) of blood free amino acid levels. This was the case when subjects were fed casein or frequent small meals [10]. The main target in this situation appears to be in the splanchnic area for both protein degradation and for protein synthesis. In humans, it is likely that the inhi ...
... increase (even moderate) of blood free amino acid levels. This was the case when subjects were fed casein or frequent small meals [10]. The main target in this situation appears to be in the splanchnic area for both protein degradation and for protein synthesis. In humans, it is likely that the inhi ...
PPT - Portland State University
... in n-3, or ω-3, fatty acids lead to the replacement of 22:6n-3 with 22:5n6. Dietary n-3 deficiency leads to a spectrum of developmental disorders associated with learning, memory, intelligence, and visual function. We examined rhodopsin, transducin (Gt) and phosphodiesterase (PDE) function and acyl ...
... in n-3, or ω-3, fatty acids lead to the replacement of 22:6n-3 with 22:5n6. Dietary n-3 deficiency leads to a spectrum of developmental disorders associated with learning, memory, intelligence, and visual function. We examined rhodopsin, transducin (Gt) and phosphodiesterase (PDE) function and acyl ...
Regulation of microtubule dynamics by microtubule
... to control microtubule dynamics in vivo. There is a major group of microtubule-interacting proteins that have ATPase activity and transiently bind to tubulin. These «motor» proteins are involved in the transport of organelles along microtubules. As these proteins do not show a stable association wit ...
... to control microtubule dynamics in vivo. There is a major group of microtubule-interacting proteins that have ATPase activity and transiently bind to tubulin. These «motor» proteins are involved in the transport of organelles along microtubules. As these proteins do not show a stable association wit ...
The Ribosome, rRNA and mRNA (3.1)
... The large ribosomal subunit catalyzes peptide bond formation and binds initiation, termination, and elongation factors. We have determined the crystal structure of the large ribosomal subunit from Haloarcula marismortui at 2.4 angstrom resolution, and it includes 2833 of the subunit's 3045 nucleotid ...
... The large ribosomal subunit catalyzes peptide bond formation and binds initiation, termination, and elongation factors. We have determined the crystal structure of the large ribosomal subunit from Haloarcula marismortui at 2.4 angstrom resolution, and it includes 2833 of the subunit's 3045 nucleotid ...
Protein synthesis (Primer)
... There is a correlation with the non-polar solvent accessible surface area The interaction with the surrounding beta structure is a dominant force for determining beta sheet propensity—i.e. the tertiary structure is an important determinant of beta sheet propensity ...
... There is a correlation with the non-polar solvent accessible surface area The interaction with the surrounding beta structure is a dominant force for determining beta sheet propensity—i.e. the tertiary structure is an important determinant of beta sheet propensity ...
PPT
... • Assign all of the residues in the peptide the appropriate set of parameters. • Scan through the peptide and identify regions where 4 out of 6 contiguous residues have P(a-helix) > 100. • That region is declared an alpha-helix. Extend the helix in both directions until a set of four contiguous resi ...
... • Assign all of the residues in the peptide the appropriate set of parameters. • Scan through the peptide and identify regions where 4 out of 6 contiguous residues have P(a-helix) > 100. • That region is declared an alpha-helix. Extend the helix in both directions until a set of four contiguous resi ...
Jürgen Sühnel 3D Structures of Biological Macromolecules
... • Assign all of the residues in the peptide the appropriate set of parameters. • Scan through the peptide and identify regions where 4 out of 6 contiguous residues have P(a-helix) > 100. • That region is declared an alpha-helix. Extend the helix in both directions until a set of four contiguous resi ...
... • Assign all of the residues in the peptide the appropriate set of parameters. • Scan through the peptide and identify regions where 4 out of 6 contiguous residues have P(a-helix) > 100. • That region is declared an alpha-helix. Extend the helix in both directions until a set of four contiguous resi ...
Storage globulins pass through the Golgi apparatus and
... storage vacuoles (PSVs). Multiple mechanisms have been proposed to be responsible for transporting storage proteins to PSVs in developing seeds. In this study, a specific antibody was raised against the mung bean (Vigna radiata) seed storage protein 8S globulin and its deposition was followed via im ...
... storage vacuoles (PSVs). Multiple mechanisms have been proposed to be responsible for transporting storage proteins to PSVs in developing seeds. In this study, a specific antibody was raised against the mung bean (Vigna radiata) seed storage protein 8S globulin and its deposition was followed via im ...
G protein–coupled receptor
G protein–coupled receptors (GPCRs), also known as seven-transmembrane domain receptors, 7TM receptors, heptahelical receptors, serpentine receptor, and G protein–linked receptors (GPLR), constitute a large protein family of receptors that sense molecules outside the cell and activate inside signal transduction pathways and, ultimately, cellular responses. Coupling with G proteins, they are called seven-transmembrane receptors because they pass through the cell membrane seven times.G protein–coupled receptors are found only in eukaryotes, including yeast, choanoflagellates, and animals. The ligands that bind and activate these receptors include light-sensitive compounds, odors, pheromones, hormones, and neurotransmitters, and vary in size from small molecules to peptides to large proteins. G protein–coupled receptors are involved in many diseases, and are also the target of approximately 40% of all modern medicinal drugs. Two of the United States's top five selling drugs (Hydrocodone and Lisinopril) act by targeting a G protein–coupled receptor. The 2012 Nobel Prize in Chemistry was awarded to Brian Kobilka and Robert Lefkowitz for their work that was ""crucial for understanding how G protein–coupled receptors function."". There have been at least seven other Nobel Prizes awarded for some aspect of G protein–mediated signaling.There are two principal signal transduction pathways involving the G protein–coupled receptors: the cAMP signal pathway and the phosphatidylinositol signal pathway. When a ligand binds to the GPCR it causes a conformational change in the GPCR, which allows it to act as a guanine nucleotide exchange factor (GEF). The GPCR can then activate an associated G protein by exchanging its bound GDP for a GTP. The G protein's α subunit, together with the bound GTP, can then dissociate from the β and γ subunits to further affect intracellular signaling proteins or target functional proteins directly depending on the α subunit type (Gαs, Gαi/o, Gαq/11, Gα12/13).