Cellular Disulfide Bond Formation in Bioactive Peptides and
... cofactor [73] which enables the generation of disulfide bonds. It also contains thioredoxin [74] motifs which aid in transferring the disulfide to the substrate protein [75]. In mammals, cells secrete QSOXs into the extracellular space after modification by the Golgi complex [76]. Human QSOX1 has tw ...
... cofactor [73] which enables the generation of disulfide bonds. It also contains thioredoxin [74] motifs which aid in transferring the disulfide to the substrate protein [75]. In mammals, cells secrete QSOXs into the extracellular space after modification by the Golgi complex [76]. Human QSOX1 has tw ...
The TEA Transcription Factor Tec1 Links TOR and MAPK Pathways
... that is independent of the CPD, but involves the C-terminal part of Tec1 (Chou et al. 2006; Heise et al. 2010). In S. cerevisiae, cell division and cellular development are also controlled by the nutrient-sensitive TORC1 signaling network, which is negatively regulated by the macrocyclic lactone rap ...
... that is independent of the CPD, but involves the C-terminal part of Tec1 (Chou et al. 2006; Heise et al. 2010). In S. cerevisiae, cell division and cellular development are also controlled by the nutrient-sensitive TORC1 signaling network, which is negatively regulated by the macrocyclic lactone rap ...
Protein Kinase C inhibits Kv1.1 potassium channel function
... in ventricular cells (36). In the Xenopus oocyte expression system, several types of recombinant, voltagegated K channels have been shown to be modulated by PKC (18, 19, 21, 23, 29), and in some cases there is evidence that the K channel protein is the substrate for PKC phosphorylation (2, 3, 4, 8). ...
... in ventricular cells (36). In the Xenopus oocyte expression system, several types of recombinant, voltagegated K channels have been shown to be modulated by PKC (18, 19, 21, 23, 29), and in some cases there is evidence that the K channel protein is the substrate for PKC phosphorylation (2, 3, 4, 8). ...
Solvent Accessibility Prediction in proteins
... hence crucial to stability, even if they may not be active sites. • A protein may lose function upon mutation either because the new amino acid does not bind or because the protein lost structure upon that mutation making stability a crucial factor. • Accessible residues represent active sites of th ...
... hence crucial to stability, even if they may not be active sites. • A protein may lose function upon mutation either because the new amino acid does not bind or because the protein lost structure upon that mutation making stability a crucial factor. • Accessible residues represent active sites of th ...
Overview for Electrophoresis and Western Blotting
... The discontinuous buffer systems employ different buffer ions and pH in the gel and in the electrode reservoirs. Samples are loaded onto a non-restrictive large pore gel, called a stacking gel, which overlays a smaller pore resolving gel. The major advantages of discontinuous buffer systems are that ...
... The discontinuous buffer systems employ different buffer ions and pH in the gel and in the electrode reservoirs. Samples are loaded onto a non-restrictive large pore gel, called a stacking gel, which overlays a smaller pore resolving gel. The major advantages of discontinuous buffer systems are that ...
PAI-1 - Trace: Tennessee Research and Creative Exchange
... imidazole buffer and applied an imidazole gradient to compete with histidine binding for release of bound proteins. The fractions were read to create and elution profile, and we analyzed by SDS-PAGE and Western blot. The protein was then concentrated to 2-3 milliliters volume and loaded on S-100 fil ...
... imidazole buffer and applied an imidazole gradient to compete with histidine binding for release of bound proteins. The fractions were read to create and elution profile, and we analyzed by SDS-PAGE and Western blot. The protein was then concentrated to 2-3 milliliters volume and loaded on S-100 fil ...
Investigating semantic similarity measures across the
... another term. These orphan terms within GO need to be provided with links for the purposes of our investigation. We simply linked them directly to the root of their taxonomy. This is perhaps semantically impoverished (for example, a “granum”, (GO:0009542) becomes a kind of “cellular component”, (GO: ...
... another term. These orphan terms within GO need to be provided with links for the purposes of our investigation. We simply linked them directly to the root of their taxonomy. This is perhaps semantically impoverished (for example, a “granum”, (GO:0009542) becomes a kind of “cellular component”, (GO: ...
A first attempt to elucidate the amino acid sequence of some lichen
... induce ultrastructure damages such as chloroplast disorganization, hydrolysis of the cell wall and, finally, cell death (Molina et al., 1998). Increases in polyamine concentration are the result of the arginase activity of the signaling protein. On the contrary, the occurrence of the ligand at the a ...
... induce ultrastructure damages such as chloroplast disorganization, hydrolysis of the cell wall and, finally, cell death (Molina et al., 1998). Increases in polyamine concentration are the result of the arginase activity of the signaling protein. On the contrary, the occurrence of the ligand at the a ...
A Method To Define the Carboxyl Terminal of Proteins
... C-terminal sequence is achieved by capillary-HPLC electrospray ionization tandem mass spectrometry of the isolated peptide using a quadrupole ion trap mass spectrometer in the selective reaction monitoring mode. This strategy was successfully applied to the characterization of the C-terminal of prot ...
... C-terminal sequence is achieved by capillary-HPLC electrospray ionization tandem mass spectrometry of the isolated peptide using a quadrupole ion trap mass spectrometer in the selective reaction monitoring mode. This strategy was successfully applied to the characterization of the C-terminal of prot ...
R26 :: CAG GCaMP6f - The Jackson Laboratory
... international biomedical research community - adds hundreds of new strains annually. The JAX Mouse Repository is supported by NIH, The Howard Hughes Medical Institute and several private charitable foundations. ...
... international biomedical research community - adds hundreds of new strains annually. The JAX Mouse Repository is supported by NIH, The Howard Hughes Medical Institute and several private charitable foundations. ...
DMD # 35568 - Drug Metabolism and Disposition
... This article has not been copyedited and formatted. The final version may differ from this version. ...
... This article has not been copyedited and formatted. The final version may differ from this version. ...
Localization of Low-sulfur Keratin Proteins in the Wool Follicle Using
... using indirect immunofluorescent. The results are listed in Table I and shown schematically in Fig. 3. Despite the disadvantage that the monoclonal antibodies all recognize more than one protein component, the studies indicate a specific pattern of antibody binding to wool follicles. All of the seve ...
... using indirect immunofluorescent. The results are listed in Table I and shown schematically in Fig. 3. Despite the disadvantage that the monoclonal antibodies all recognize more than one protein component, the studies indicate a specific pattern of antibody binding to wool follicles. All of the seve ...
Supplementary Figures 1 - 5, Methods
... The TRAX protein is known to associate with the C-terminal region of PLC1, ...
... The TRAX protein is known to associate with the C-terminal region of PLC1, ...
Differential display proteomic analysis of Picea meyeri pollen
... cytoskeleton in living cells (Gibbon et al., 1999). Furthermore, at the biochemical level, LATB has a well understood and simple mode of action resulting in a complete shift from F-actin to G-actin (Walter et al., 2000). In recent years, LATB has been widely used to investigate the function of actin ...
... cytoskeleton in living cells (Gibbon et al., 1999). Furthermore, at the biochemical level, LATB has a well understood and simple mode of action resulting in a complete shift from F-actin to G-actin (Walter et al., 2000). In recent years, LATB has been widely used to investigate the function of actin ...
Proteins - The Open University
... Chemical catalysis Enzymes, which are responsible for catalysing biological reactions, are the largest functional group of proteins. Whilst there are thousands of different enzymes, all catalysing different reactions, they do have some features in common and can often be identified as members of a p ...
... Chemical catalysis Enzymes, which are responsible for catalysing biological reactions, are the largest functional group of proteins. Whilst there are thousands of different enzymes, all catalysing different reactions, they do have some features in common and can often be identified as members of a p ...
Flavors of Protein Disorder
... ago7, and that some proteins don’t fold due to an atypical amino acid composition was suggested more than 20 years ago8, 9. More recently, such proteins have been called "natively unfolded"10, "intrinsically unstructured"1, and “intrinsically disordered”2. The failure to self-fold into specific 3-D ...
... ago7, and that some proteins don’t fold due to an atypical amino acid composition was suggested more than 20 years ago8, 9. More recently, such proteins have been called "natively unfolded"10, "intrinsically unstructured"1, and “intrinsically disordered”2. The failure to self-fold into specific 3-D ...
pdf
... a. Ribosomes are the molecular machines that catalyze peptide bond formation between a growing polypeptide and an incoming aminoacyl-tRNA. The ribosomes insures that the amino acids are added in the order specified by the mRNA. b. Ribosomes associate reversibly with the mRNA. The two subunits of the ...
... a. Ribosomes are the molecular machines that catalyze peptide bond formation between a growing polypeptide and an incoming aminoacyl-tRNA. The ribosomes insures that the amino acids are added in the order specified by the mRNA. b. Ribosomes associate reversibly with the mRNA. The two subunits of the ...
Western blot Fast purification Comparative performance
... detection method for studying proteins. The typical protocol for measuring chemiluminescent signal exposes the Western blot to laboratory-grade X-ray film, but digital imaging using a cooledCCD camera is proving to be a more functional choice for researchers. The Thermo Scientific myECL Imager offer ...
... detection method for studying proteins. The typical protocol for measuring chemiluminescent signal exposes the Western blot to laboratory-grade X-ray film, but digital imaging using a cooledCCD camera is proving to be a more functional choice for researchers. The Thermo Scientific myECL Imager offer ...
Identification of fungal oxaloacetate hydrolyase within the
... Aspergillus niger produces oxalic acid through the hydrolysis of oxaloacetate, catalyzed by the cytoplasmic enzyme oxaloacetate acetylhydrolase (OAH). The A. niger genome encodes four additional open reading frames with strong sequence similarity to OAH yet only the oahA gene encodes OAH activity. O ...
... Aspergillus niger produces oxalic acid through the hydrolysis of oxaloacetate, catalyzed by the cytoplasmic enzyme oxaloacetate acetylhydrolase (OAH). The A. niger genome encodes four additional open reading frames with strong sequence similarity to OAH yet only the oahA gene encodes OAH activity. O ...
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Lecture 6 (ADP/ATP carrier) []
... Contains more than 100 different polypeptides, with a very high proteinto-phospholipid ratio (more than 3:1 by weight, which is about 1 protein for 15 phospholipids). Additionally, the inner membrane is rich in an unusual phospholipid, cardiolipin. Unlike the outer membrane, the inner membrane does ...
... Contains more than 100 different polypeptides, with a very high proteinto-phospholipid ratio (more than 3:1 by weight, which is about 1 protein for 15 phospholipids). Additionally, the inner membrane is rich in an unusual phospholipid, cardiolipin. Unlike the outer membrane, the inner membrane does ...
Introduction - ART
... ubiquitin-tagged proteins; ubiquitination also regulates certain processes by mechanisms that, although poorly understood, do not appear to involve proteolysis. These processes include ribosomal function (Spence et al. 2000), postreplicational DNA repair (Spence et al. 1995), the initiation of the i ...
... ubiquitin-tagged proteins; ubiquitination also regulates certain processes by mechanisms that, although poorly understood, do not appear to involve proteolysis. These processes include ribosomal function (Spence et al. 2000), postreplicational DNA repair (Spence et al. 1995), the initiation of the i ...
Role of cryo-ET in membrane bioenergetics research
... densities attributed to PSII in thylakoid membranes (right) and the atomic model of PSII, PDB code 2O01 filtered to 30 Å (1 Å = 0.1 nm) (surface view, left; slice through volume, middle). Both densities are roughly the same size, two-fold symmetric and have two kidney-shaped protrusions on one side o ...
... densities attributed to PSII in thylakoid membranes (right) and the atomic model of PSII, PDB code 2O01 filtered to 30 Å (1 Å = 0.1 nm) (surface view, left; slice through volume, middle). Both densities are roughly the same size, two-fold symmetric and have two kidney-shaped protrusions on one side o ...
Variation in Glutenin Protein Subunits of Wheat
... bands than single-gel systems, we decided to use such a system to separate the glutenin subunits with the aim of determining more precisely the degree of variation that exists for glutenin subunits in cultivars of hexaploid wheat. This paper reports the results of using such a system to separate the ...
... bands than single-gel systems, we decided to use such a system to separate the glutenin subunits with the aim of determining more precisely the degree of variation that exists for glutenin subunits in cultivars of hexaploid wheat. This paper reports the results of using such a system to separate the ...
Dynamic regulation of the GABAA receptor function by redox
... excitatory and inhibitory receptors, have not been extensively studied. Ascorbate exerts diverse neuromodulatory actions including redox modulation of synaptic receptors (Majewska et al., 1990; Calero et al., 2011), either directly by reducing amino acidic residues or indirectly by scavenging ROS ca ...
... excitatory and inhibitory receptors, have not been extensively studied. Ascorbate exerts diverse neuromodulatory actions including redox modulation of synaptic receptors (Majewska et al., 1990; Calero et al., 2011), either directly by reducing amino acidic residues or indirectly by scavenging ROS ca ...
Properties of a newly characterized protein of the bovine - K-REx
... covalently bound in an amide linkage to the transacetylase subunit through the e-amino group of a lysine. The remaining approximately 300 amino acids contain the capacity to bind subunits and to catalyze the ...
... covalently bound in an amide linkage to the transacetylase subunit through the e-amino group of a lysine. The remaining approximately 300 amino acids contain the capacity to bind subunits and to catalyze the ...
G protein–coupled receptor
G protein–coupled receptors (GPCRs), also known as seven-transmembrane domain receptors, 7TM receptors, heptahelical receptors, serpentine receptor, and G protein–linked receptors (GPLR), constitute a large protein family of receptors that sense molecules outside the cell and activate inside signal transduction pathways and, ultimately, cellular responses. Coupling with G proteins, they are called seven-transmembrane receptors because they pass through the cell membrane seven times.G protein–coupled receptors are found only in eukaryotes, including yeast, choanoflagellates, and animals. The ligands that bind and activate these receptors include light-sensitive compounds, odors, pheromones, hormones, and neurotransmitters, and vary in size from small molecules to peptides to large proteins. G protein–coupled receptors are involved in many diseases, and are also the target of approximately 40% of all modern medicinal drugs. Two of the United States's top five selling drugs (Hydrocodone and Lisinopril) act by targeting a G protein–coupled receptor. The 2012 Nobel Prize in Chemistry was awarded to Brian Kobilka and Robert Lefkowitz for their work that was ""crucial for understanding how G protein–coupled receptors function."". There have been at least seven other Nobel Prizes awarded for some aspect of G protein–mediated signaling.There are two principal signal transduction pathways involving the G protein–coupled receptors: the cAMP signal pathway and the phosphatidylinositol signal pathway. When a ligand binds to the GPCR it causes a conformational change in the GPCR, which allows it to act as a guanine nucleotide exchange factor (GEF). The GPCR can then activate an associated G protein by exchanging its bound GDP for a GTP. The G protein's α subunit, together with the bound GTP, can then dissociate from the β and γ subunits to further affect intracellular signaling proteins or target functional proteins directly depending on the α subunit type (Gαs, Gαi/o, Gαq/11, Gα12/13).