Lecture 5. Confocal microscopy and instrumentation I
... Very good quality laser beam from low power He-Ne laser can have a M2 ~1.05. Most lasers does not have such ideal beam. ...
... Very good quality laser beam from low power He-Ne laser can have a M2 ~1.05. Most lasers does not have such ideal beam. ...
In situ Raman Spectroscopic Study of Supported Molten Salt
... giving rise to formation of vanadium oxosulfato complexes ...
... giving rise to formation of vanadium oxosulfato complexes ...
Surface Watch - YMS Magazine
... microscope (AFM) measurements at discrete points. Process window characterization for grain size of interconnect films and unprecedented bare substrate microroughness characterization are now possible, as well as excursion monitoring and process control based on surface topography. Previously, surfa ...
... microscope (AFM) measurements at discrete points. Process window characterization for grain size of interconnect films and unprecedented bare substrate microroughness characterization are now possible, as well as excursion monitoring and process control based on surface topography. Previously, surfa ...
Low-Cost Tunable Adaptive Optics.pdf
... beam. Bessel beams are nondiffracting and self-healing. Because of these properties, they have uses in optical micromanipulation, where they can form two dimensional traps and transport microscopic particles over long distances. Bessel beams are also used in laser materials processing to process une ...
... beam. Bessel beams are nondiffracting and self-healing. Because of these properties, they have uses in optical micromanipulation, where they can form two dimensional traps and transport microscopic particles over long distances. Bessel beams are also used in laser materials processing to process une ...
Assessing age-related changes in the biomechanical properties of
... The increase in lens stiffness is generally believed to be responsible for the progressive loss of the ability of the lens to change shape leading to presbyopia. The location of the crystalline lens inside the eye makes it challenging to measure its mechanical properties in vivo or in situ. ...
... The increase in lens stiffness is generally believed to be responsible for the progressive loss of the ability of the lens to change shape leading to presbyopia. The location of the crystalline lens inside the eye makes it challenging to measure its mechanical properties in vivo or in situ. ...
18.1 Raman Scattering
... where ε0 is the permittivity of free space and χ is the electric susceptibility. χ has values from 0 to ∞, and varies with density. A vacuum has χ = 0, while a metal will have a very large value. Because the electric vector of light is oscillatory, the polarized electrons will oscillate at the optic ...
... where ε0 is the permittivity of free space and χ is the electric susceptibility. χ has values from 0 to ∞, and varies with density. A vacuum has χ = 0, while a metal will have a very large value. Because the electric vector of light is oscillatory, the polarized electrons will oscillate at the optic ...
ps fiber laser for CARS-authorproof-v2
... shorter pulses offer high peak power (and therefore signal) but limited spectral resolution, while longer pulses generate less signal but with better spectral resolution and specificity [4]. In practice, picosecond excitation is preferred to match the spectral width of the laser source to the intrin ...
... shorter pulses offer high peak power (and therefore signal) but limited spectral resolution, while longer pulses generate less signal but with better spectral resolution and specificity [4]. In practice, picosecond excitation is preferred to match the spectral width of the laser source to the intrin ...
light microscopy
... object is, in fact, a cross section of a three-dimensional pattern that extends along the optical axis of the microscope . As one focuses an objective lens for short distances above and below exact focus, the brightness of the central spot periodically oscillates between bright and dark as its absol ...
... object is, in fact, a cross section of a three-dimensional pattern that extends along the optical axis of the microscope . As one focuses an objective lens for short distances above and below exact focus, the brightness of the central spot periodically oscillates between bright and dark as its absol ...
Confocal Live Cell Microscopy
... specimens are then mounted between an objective slide and a cover slip. Live cell imaging brings along a variety of benefits not possible with fixed samples: observation of dynamic changes, reliable 3D structures, avoidance of fixation artefacts etc. Live cell imaging sets certain criteria also for ...
... specimens are then mounted between an objective slide and a cover slip. Live cell imaging brings along a variety of benefits not possible with fixed samples: observation of dynamic changes, reliable 3D structures, avoidance of fixation artefacts etc. Live cell imaging sets certain criteria also for ...
Detection Sensitivity of Single-Molecule Optical Absorption at Room
... surpassed by much easier far-field single molecule imaging with total internal reflection12 and confocal microscopy.1,13 Surface enhanced Raman scattering is capable of detecting single molecules,14,15 but it requires close contact of molecules with a metal nanostructure, which is difficult to contr ...
... surpassed by much easier far-field single molecule imaging with total internal reflection12 and confocal microscopy.1,13 Surface enhanced Raman scattering is capable of detecting single molecules,14,15 but it requires close contact of molecules with a metal nanostructure, which is difficult to contr ...
Assignment #2 - Rose
... in the z = 0 plane, with the beam waist radii w0x and w0y in the x and y-directions respectively. The contours of constant intensity are therefore ellipses instead of circles. Write expressions for the beam depth of focus, angular divergence, and radii of curvature in the x and y-directions, as func ...
... in the z = 0 plane, with the beam waist radii w0x and w0y in the x and y-directions respectively. The contours of constant intensity are therefore ellipses instead of circles. Write expressions for the beam depth of focus, angular divergence, and radii of curvature in the x and y-directions, as func ...
CH915: Elemental Analysis
... dissociate in the flame Ionisation of the analyte can reduce the signal Matrix interferences due to differences between surface tension and viscosity of test solutions and ...
... dissociate in the flame Ionisation of the analyte can reduce the signal Matrix interferences due to differences between surface tension and viscosity of test solutions and ...
H2 Raman overtone intensities measured for
... spectral response function of the spectrometer. For a flat spectral distribution, the measured count rate is proportional to the product of spectral intensity per unit frequency and spectral slit width av, while for a monochromatic source, the integrated count obtained by scanning the spectral line ...
... spectral response function of the spectrometer. For a flat spectral distribution, the measured count rate is proportional to the product of spectral intensity per unit frequency and spectral slit width av, while for a monochromatic source, the integrated count obtained by scanning the spectral line ...
Raman Scattering in Solids
... The backscattered light is collected with an objective lens (fig. 8) with large aperture and focused on the entrance slit of a highly dispersive and selective grating spectrometer. In spite of the good selectivity of double and triple monochromators elastically scattered laser light cannot be comple ...
... The backscattered light is collected with an objective lens (fig. 8) with large aperture and focused on the entrance slit of a highly dispersive and selective grating spectrometer. In spite of the good selectivity of double and triple monochromators elastically scattered laser light cannot be comple ...
Light Scattering Spectroscopy
... Summary of LSS • LSS contrasts: • Polarization: single vs. multiple scattering • Angle: small vs. large particles • Spectrum: size and refractive index • Advantages: • Strong signal - allows use of lower cost components components. • Sensitive to important chromophores that are not fluorescent: e.g ...
... Summary of LSS • LSS contrasts: • Polarization: single vs. multiple scattering • Angle: small vs. large particles • Spectrum: size and refractive index • Advantages: • Strong signal - allows use of lower cost components components. • Sensitive to important chromophores that are not fluorescent: e.g ...
INTRODUCTION TO SPECTROSCOPIC METHODS OF ANALYSIS
... and sends it to a readout device such as a meter, digital readout, chart recorder, computer, etc. ...
... and sends it to a readout device such as a meter, digital readout, chart recorder, computer, etc. ...
The Interaction of Radiation and Matter: Quantum
... When the normal mode is driven by a two (visible) wave input (see discussion of fourwave processes above), its dominant response will be at the difference frequency -- viz. ...
... When the normal mode is driven by a two (visible) wave input (see discussion of fourwave processes above), its dominant response will be at the difference frequency -- viz. ...
Argos TM - Lockheed Martin
... Figure 1: The QEPAS tuning-fork sensor. With QEPAS, researchers measure the amplitude of the sound wave that is generated when gas molecules absorb modulated light. The detection module is a low-cost, compact, quartz tuning fork, such as those commonly found in digital watches. The QEPAS sensor has ...
... Figure 1: The QEPAS tuning-fork sensor. With QEPAS, researchers measure the amplitude of the sound wave that is generated when gas molecules absorb modulated light. The detection module is a low-cost, compact, quartz tuning fork, such as those commonly found in digital watches. The QEPAS sensor has ...
MICROSCOPY
... covers two main types of diffraction-limited microscopy. The first provides optical visualization plus IR spectroscopic data collection. ...
... covers two main types of diffraction-limited microscopy. The first provides optical visualization plus IR spectroscopic data collection. ...
supplemental_material
... Figure 1. Measured transmittance of sample with 2.8 nm PtSi with Black Silicon light-trapping. Illumination is from the nanostructured b-Si side of the sample. In order to estimate the rear mirror effect, additional measurements were conducted, in which the sample was flipped; such that illumination ...
... Figure 1. Measured transmittance of sample with 2.8 nm PtSi with Black Silicon light-trapping. Illumination is from the nanostructured b-Si side of the sample. In order to estimate the rear mirror effect, additional measurements were conducted, in which the sample was flipped; such that illumination ...
Extinction Coefficient Measurements of Turbid Media
... A 1 mW He–Ne laser source emitting at 632.8 nm operating in continuous wave (cw) [which has proved suitable for low-power laser interaction with matter], was directed horizontally to a cell (glass box) of thickness 25 mm containing the sample . The collimated transmitted (unscattered ) light was det ...
... A 1 mW He–Ne laser source emitting at 632.8 nm operating in continuous wave (cw) [which has proved suitable for low-power laser interaction with matter], was directed horizontally to a cell (glass box) of thickness 25 mm containing the sample . The collimated transmitted (unscattered ) light was det ...
Coherent laser radar system using optical homodyne detection
... Raman spectroscopy (RS) is a technique with potential to solve several problems in oceanographic remote sensing, including being able to provide reliable data about subsurface water properties (e.g. temperature, salinity). Several studies established a relationship between water Raman spectra and te ...
... Raman spectroscopy (RS) is a technique with potential to solve several problems in oceanographic remote sensing, including being able to provide reliable data about subsurface water properties (e.g. temperature, salinity). Several studies established a relationship between water Raman spectra and te ...
Nugget
... pathogenesis of the neurodegenerative diseases such as Alzheimer. Magnetite (Fe3O4) is an example of the Correlated Electron Materials. These materials represent an intermediate case of the electronic structure of solids between those that have itinerant electrons and those with localized electrons. ...
... pathogenesis of the neurodegenerative diseases such as Alzheimer. Magnetite (Fe3O4) is an example of the Correlated Electron Materials. These materials represent an intermediate case of the electronic structure of solids between those that have itinerant electrons and those with localized electrons. ...
1.3.6 Electromagnetic radiation Name Symbol Definition SI
... power α/γl, or the molar optical rotatory power α/cl, where γ is the mass concentration, c is the amount (of substance) concentration, and l is the path length. Most tabulations give the specific optical rotatory power, denoted [α]. The wavelength of light used λ (frequently the sodium D line) and t ...
... power α/γl, or the molar optical rotatory power α/cl, where γ is the mass concentration, c is the amount (of substance) concentration, and l is the path length. Most tabulations give the specific optical rotatory power, denoted [α]. The wavelength of light used λ (frequently the sodium D line) and t ...
I. Physical Biochemistry Protein Interactions Ultraviolet(UV
... Mathematically, each point of the protein spectra and each point of the ligand spectra are subtracted from the equivalent point of the protein-ligand spectra. The result is the UV absorbance associated with the conformational changes in the protein due to ligand target binding. ...
... Mathematically, each point of the protein spectra and each point of the ligand spectra are subtracted from the equivalent point of the protein-ligand spectra. The result is the UV absorbance associated with the conformational changes in the protein due to ligand target binding. ...