Genetic Engineering
... This process may be divided into the following steps: ● Isolation of plasmid DNA containing the cloned sequence of interest. ● Digestion (cutting) of the plasmid into discrete fragments with restriction endonucleases. ● Separation of the fragments by agarose gel electrophoresis. ● Purification of th ...
... This process may be divided into the following steps: ● Isolation of plasmid DNA containing the cloned sequence of interest. ● Digestion (cutting) of the plasmid into discrete fragments with restriction endonucleases. ● Separation of the fragments by agarose gel electrophoresis. ● Purification of th ...
Introduction_to_Enzymes (1)
... Almost all chemical reactions in an organism are controlled by enzymes. ...
... Almost all chemical reactions in an organism are controlled by enzymes. ...
I have.. Who has.. DNA produced from mRNA by reverse
... between DNA fragments by complementary base pairing ...
... between DNA fragments by complementary base pairing ...
6.2 Recombinant DNA Technology
... DNA extracted from human cells DNA treated with restriction enzyme, cuts the DNA at specific sites, produce “sticky end” Bacterial plasmid cut with same enzyme ...
... DNA extracted from human cells DNA treated with restriction enzyme, cuts the DNA at specific sites, produce “sticky end” Bacterial plasmid cut with same enzyme ...
Scientific Notebook
... 16 weeks, three hour lab meeting/week (either one 3-hour meeting or three 1-hour meeting) A total of 48 hours of lab time Each lab activity should be assigned as a reading assignment prior to the class meeting. Activities may require adjustment to meet the time limitations of a particular course. We ...
... 16 weeks, three hour lab meeting/week (either one 3-hour meeting or three 1-hour meeting) A total of 48 hours of lab time Each lab activity should be assigned as a reading assignment prior to the class meeting. Activities may require adjustment to meet the time limitations of a particular course. We ...
Notes_DNA Replication_teacher
... the new DNA strand. This means that it must constantly back track to copy parts of the strand newly exposed by helicase. Since this process takes longer than the other strand, it is called the lagging strand. The Strand that does not need to do this is called the leading strand. The fragments that a ...
... the new DNA strand. This means that it must constantly back track to copy parts of the strand newly exposed by helicase. Since this process takes longer than the other strand, it is called the lagging strand. The Strand that does not need to do this is called the leading strand. The fragments that a ...
20 - Biotechnology
... to cut strands of DNA at specific locations (restriction sites) Restriction Fragments: have at least 1 sticky end (single-stranded end) DNA ligase: joins DNA fragments Cloning vector: carries the DNA sequence to be cloned ...
... to cut strands of DNA at specific locations (restriction sites) Restriction Fragments: have at least 1 sticky end (single-stranded end) DNA ligase: joins DNA fragments Cloning vector: carries the DNA sequence to be cloned ...
Answers-to-examination-in-Gene-technology_20121020
... Change in the DNA sequence that do not cause any change in the amino acid sequence. e) A palindromic sequence: CTTTGA change to 5’-CTATAG-3’ or 5’-TTATAA-5 3’-GATATC-5’ 3’-AATATT-3’ f) The advantage is the possibility to regulate the transcription of the gene. If the gene product is toxic and harmfu ...
... Change in the DNA sequence that do not cause any change in the amino acid sequence. e) A palindromic sequence: CTTTGA change to 5’-CTATAG-3’ or 5’-TTATAA-5 3’-GATATC-5’ 3’-AATATT-3’ f) The advantage is the possibility to regulate the transcription of the gene. If the gene product is toxic and harmfu ...
DNA Structure, Replication and Protein Synthesis
... Insert the most appropriate words in each of the following spaces: A section of DNA that causes the production of a protein is called a ___________________. Sections of DNA that do not code for a particular protein are called _____________________________. The protein ____________________________ ...
... Insert the most appropriate words in each of the following spaces: A section of DNA that causes the production of a protein is called a ___________________. Sections of DNA that do not code for a particular protein are called _____________________________. The protein ____________________________ ...
Watson, Crick and Wilkins
... Holley, an organic chemist, was able to determine the structure of transfer RNA, the RNA which at one end attaches to a specific amino acid and at the other has a code complementary to that on the messenger RNA. ...
... Holley, an organic chemist, was able to determine the structure of transfer RNA, the RNA which at one end attaches to a specific amino acid and at the other has a code complementary to that on the messenger RNA. ...
PLASMIDS AND RESTRICTION ENZYMES
... lacking that plasmid, the bacteria will then become resistant to that specific antibiotic. In nature, conjugation occurs with a very low efficiency; that is, only a small percentage of bacteria in a population can take in plasmid DNA at any point in time. The presence of an antibiotic resistance gen ...
... lacking that plasmid, the bacteria will then become resistant to that specific antibiotic. In nature, conjugation occurs with a very low efficiency; that is, only a small percentage of bacteria in a population can take in plasmid DNA at any point in time. The presence of an antibiotic resistance gen ...
Genetic engineering – stepping stones
... Plasmid of bacterial DNA is cut open. Insulin gene is inserted. Altered plasmid is inserted into another bacterium. Assaying techniques are used to check new gene. Transgenic bacteria begin to produce insulin. Transgenic bacteria are cloned and cultured. Human insulin is produced in significant quan ...
... Plasmid of bacterial DNA is cut open. Insulin gene is inserted. Altered plasmid is inserted into another bacterium. Assaying techniques are used to check new gene. Transgenic bacteria begin to produce insulin. Transgenic bacteria are cloned and cultured. Human insulin is produced in significant quan ...
Chapter 9 - FIU Faculty Websites
... Complex with nonspecific DNA does not bind Mg2+ whereas complex with cognate DNA bind Mg2+ ...
... Complex with nonspecific DNA does not bind Mg2+ whereas complex with cognate DNA bind Mg2+ ...