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Molecular Evolution of Functional Nucleic Acids
Molecular Evolution of Functional Nucleic Acids

... Eaton et al. created a modified RNA enzyme catalyzing the Diels–Alder reaction from a modified RNA library [19,20]. They used modified RNA containing uridine analog with a pyridiyl group at the 5 position instead of uridine. This RNA enzyme is considered to be able to exert its catalytic activity by ...
Chronic Liver Diseases
Chronic Liver Diseases

... These assays are particularly useful for isoenzyme analysis, as antisera can be directed toward specific isoenzymes, isoforms, or subunits. ...
Divergent evolution of the thiolase superfamily and chalcone
Divergent evolution of the thiolase superfamily and chalcone

... His and Asn (or His) residues that play catalytic roles (see below) are located in the loops that converge at the active site pocket. When these residues are aligned, a similar pattern of secondary structures emerges surrounding these residues, although there is little sequence similarity. Also foun ...
Divergent Evolution of Function in the ROK Sugar
Divergent Evolution of Function in the ROK Sugar

... ABSTRACT: The D-allose and N-acetyl-D-mannosamine kinases of Escherichia coli K-12 are divergent members of the functionally diverse ROK (repressor, open reading frame, kinase) superfamily. Previous work in our laboratory has demonstrated that AlsK and NanK possess weak phosphoryl transfer activity ...
Lehninger Principles of Biochemistry
Lehninger Principles of Biochemistry

Pfx50™ DNA Polymerase - Thermo Fisher Scientific
Pfx50™ DNA Polymerase - Thermo Fisher Scientific

... resold for use in research. Invitrogen Corporation will not assert a claim against the buyer of infringement of patents owned or controlled by Invitrogen Corporation which cover this product based upon the manufacture, use or sale of a therapeutic, clinical diagnostic, vaccine or prophylactic produc ...
Metabolic Pathway Flux Enhancement by Synthetic
Metabolic Pathway Flux Enhancement by Synthetic

... have conserved folds and can more likely be used interchangeably. ...
Bio 226: Cell and Molecular Biology
Bio 226: Cell and Molecular Biology

Synthesis and isolation of a single-strand
Synthesis and isolation of a single-strand

... extension. Strand degradation and run-away synthesis commences (lanes 8-10). Note that full-length strands do not co-migrate due to their fluorophore labels. ...
Enzyme Specificity and Selectivity
Enzyme Specificity and Selectivity

... hydrophobic residues such as tryptophan, phenylalanine and tyrosine, while elastase hydrolyses peptides at small aliphatic residues such as alanine and valine. Thus, the products of enzyme-catalysed peptide hydrolysis will vary greatly depending upon the enzyme utilized. The serine protease reaction ...
24.5 Nucleic Acids
24.5 Nucleic Acids

Roles of phosphatidate phosphatase enzymes in lipid metabolism
Roles of phosphatidate phosphatase enzymes in lipid metabolism

... the generation or degradation of lipid-signaling molecules in eukaryotic cells. They are classified as either Mg2+-dependent (referred to as PAP1 enzymes) or Mg2+independent (PAP2 or lipid phosphate phosphatase [LPP] enzymes) with respect to their cofactor requirement for catalytic activity [2–7]. I ...
The DnaE polymerase from Deinococcus radiodurans features
The DnaE polymerase from Deinococcus radiodurans features

... (αDr). The αDr enzyme was overexpressed in Escherichia coli, both in soluble form and as inclusion bodies. When purified from soluble protein extracts, αDr was found to be tightly associated with E. coli RNA polymerase, from which αDr could not be dissociated. On the contrary, when refolded from inc ...
Enzymes - Exercise 3 - Science Learning Center
Enzymes - Exercise 3 - Science Learning Center

... control; do not add enzyme to this tube. • 13. Using a fresh tip, add 100uL of enzyme to each of the remaining tubes, letting the tip touch the side of the rest tube as your dispense. • 14. Collect DNS reagent from your instructor if you have not done so. Set to appropriate 1,000uL (1mL), and add a ...
The Wizard® SV 96 PCR Clean-Up System
The Wizard® SV 96 PCR Clean-Up System

... PCR(b) products directly from an amplification reaction with up to 90% recovery (Figure 1). PCR products are commonly purified to remove excess nucleotides and primers. This membrane-based system allows recovery of the PCR fragments in as little as 20 minutes. The DNA can be used for automated fluor ...
Nucleotide Sequence of fruA, the Gene Specifying Enzyme IIfru of
Nucleotide Sequence of fruA, the Gene Specifying Enzyme IIfru of

Chapter Nineteen
Chapter Nineteen

... ► The specificity of an enzyme for one of two enantiomers is a matter of fit. A chiral reactant fits a chiral reaction site. The enantiomer at the top fits the reaction site like a hand in a glove, but the enantiomer at the bottom does not. ► The enzyme lactate dehydrogenase catalyzes the removal o ...
reactants -> products. - University of San Diego Home Pages
reactants -> products. - University of San Diego Home Pages

... Rate of reaction is dependent on concentration of both A and B. Enzyme Kinetics Kinetic defn.: Of or relating to or produced by motion. Kinetics defn.: the branch of chemistry or biochemistry concerned with measuring and studying the rates of reactions. Enzyme kinetics are defined differently than b ...
An improved protocol for pulsed-field gel electrophoresis typing of
An improved protocol for pulsed-field gel electrophoresis typing of

... ribotyping scheme (Bidet et al., 2000), as a result of which we do not know whether our non-typable strains belong to those reported major groups. With the described modifications to the protocol we increased the typability of the technique, enabling all our C. difficile isolates to be characterized ...
PDF
PDF

... and GH42 enzymes characterized in this study suggest that D. turgidum can utilize most plant-based polysaccharides except crystalline cellulose. The DNA polymerase I enzyme was also expressed and characterized. The pure enzyme showed improved amplification of long PCR targets compared to Taq polymer ...
tb_ch21
tb_ch21

The ADP-dependent sugar kinase family
The ADP-dependent sugar kinase family

... protein superfamily from a structural perspective. The main efforts in this respect have been focused in the relationships within the ATP-dependent sugar kinases such as ribokinase, adenosine kinase, and 6-phosphofructokinases. Zhang et al. (5) had proposed an evolutionary hypothesis for the superfa ...
Comparison of real-time PCR with SYBR Green I or
Comparison of real-time PCR with SYBR Green I or

A Human Centromere Protein, CENP-B, Has a DNA Binding Domain
A Human Centromere Protein, CENP-B, Has a DNA Binding Domain

... the NH2-terminal part of CENP-B protein and 1.5 kb of its upstream region. The T7 expression system was used to express CENP-B in Escherichia coli (Studier et al., 1990). We first chemically synthesized the nucleotide sequence covering from the start codon to the first AccIII site of CENP-B gene dow ...
Three multidomain esterases from the cellulolytic
Three multidomain esterases from the cellulolytic

... All five include at least one potential calcium-binding motif, and show limited homology with dockerins from cellulolytic Clostridium spp. (Bayer et al., 1998a). A phylogenetic comparison including typical type I (LicB) and type II (CipA) dockerins from C. thermocellum is shown in Fig. 5. It is clea ...
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Restriction enzyme

A restriction enzyme or restriction endonuclease is an enzyme that cuts DNA at or near specific recognition nucleotide sequences known as restriction sites. Restriction enzymes are commonly classified into three types, which differ in their structure and whether they cut their DNA substrate at their recognition site, or if the recognition and cleavage sites are separate from one another. To cut DNA, all restriction enzymes make two incisions, once through each sugar-phosphate backbone (i.e. each strand) of the DNA double helix.These enzymes are found in bacteria and archaea and provide a defense mechanism against invading viruses. Inside a prokaryote, the restriction enzymes selectively cut up foreign DNA in a process called restriction; while host DNA is protected by a modification enzyme (a methyltransferase) that modifies the prokaryotic DNA and blocks cleavage. Together, these two processes form the restriction modification system.Over 3000 restriction enzymes have been studied in detail, and more than 600 of these are available commercially. These enzymes are routinely used for DNA modification in laboratories, and are a vital tool in molecular cloning.
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