The Citric acid cycle
... 1 Enzymatic reactions rates are limited by diffusion, with shorter distance between subunits a enzyme can almost direct the substrate from one subunit (catalytic site) to another. 2. Channeling metabolic intermediates between successive enzymes minimizes side reactions ...
... 1 Enzymatic reactions rates are limited by diffusion, with shorter distance between subunits a enzyme can almost direct the substrate from one subunit (catalytic site) to another. 2. Channeling metabolic intermediates between successive enzymes minimizes side reactions ...
Cress and Potato Soluble Epoxide Hydrolases
... R-250. Electrofocusing was performed with pH 3.0 –7.0 gradient gels by using precast gels and standard procedures from Novex (San Diego, CA). The molecular weight associated with EH activity of the purified enzymes was estimated from the elution profile on a gel filtration column. The enzyme (0.5 ml ...
... R-250. Electrofocusing was performed with pH 3.0 –7.0 gradient gels by using precast gels and standard procedures from Novex (San Diego, CA). The molecular weight associated with EH activity of the purified enzymes was estimated from the elution profile on a gel filtration column. The enzyme (0.5 ml ...
Pdf - Text of NPTEL IIT Video Lectures
... Apoenzymes are enzyme without its cofactor, and Holoenzyme when you combine cofactor and Apoenzyme to give rise to complete catalytic enzyme that is Holoenzyme. Most enzymes are made up of a protein part known as the Apoenzyme as well as cofactor, which can either be an organic molecule known as coe ...
... Apoenzymes are enzyme without its cofactor, and Holoenzyme when you combine cofactor and Apoenzyme to give rise to complete catalytic enzyme that is Holoenzyme. Most enzymes are made up of a protein part known as the Apoenzyme as well as cofactor, which can either be an organic molecule known as coe ...
lec-08-handout
... Thiamine pyrophosphate as its coenzyme while carbonic anhydrase uses zinc ion as its cofactor. Enzymes are classified on the basis of the reactions that they catalyze. Most enzymes are named by adding the suffix ‘ase’ either to their substrate or the type of activity they carry out. However as more ...
... Thiamine pyrophosphate as its coenzyme while carbonic anhydrase uses zinc ion as its cofactor. Enzymes are classified on the basis of the reactions that they catalyze. Most enzymes are named by adding the suffix ‘ase’ either to their substrate or the type of activity they carry out. However as more ...
to the PDF file. - CURVE
... necessitates the development of TFM derivatives that are highly selective for TvMGL so that activation of the prodrug will be limited to the parasitic cells. An essential step in the development of selective TFM-based prodrugs is mapping of the TvMGL active site to identify the residues that partici ...
... necessitates the development of TFM derivatives that are highly selective for TvMGL so that activation of the prodrug will be limited to the parasitic cells. An essential step in the development of selective TFM-based prodrugs is mapping of the TvMGL active site to identify the residues that partici ...
Extended spectrum beta-lactamases - Micro-Rao
... First instance of a plasmid-mediated beta-lactamase was reported from an ampicillin resistant E. coli isolate obtained from urine specimen of a woman named Temoniera in Greece in 1965. This enzyme was designated as TEM-1. Within a decade this enzyme had spread to several members of Enterobacteriacea ...
... First instance of a plasmid-mediated beta-lactamase was reported from an ampicillin resistant E. coli isolate obtained from urine specimen of a woman named Temoniera in Greece in 1965. This enzyme was designated as TEM-1. Within a decade this enzyme had spread to several members of Enterobacteriacea ...
Practice Exam Spring 2008 answers
... Legge Practice Exam BCHS 3304 Fall 2005 Note: This homework will not be collected. However, quizzes and exams will assume that you have completed and understand the homework assignment and could answer related questions. In this homework you are also given a practice exam II, which you may use as a ...
... Legge Practice Exam BCHS 3304 Fall 2005 Note: This homework will not be collected. However, quizzes and exams will assume that you have completed and understand the homework assignment and could answer related questions. In this homework you are also given a practice exam II, which you may use as a ...
Semester 3 - Kurukshetra University
... be set taking three questions from each unit. The candidates will be required to attempt Q.No.1 & four others selecting two questions from each unit. All questions carry equal marks. UNIT-1 Enzymes: Historical perspectives, general characteristics, nomenclature & classification, significance of numb ...
... be set taking three questions from each unit. The candidates will be required to attempt Q.No.1 & four others selecting two questions from each unit. All questions carry equal marks. UNIT-1 Enzymes: Historical perspectives, general characteristics, nomenclature & classification, significance of numb ...
In the light of the haloarchaea metabolism
... Starch is an important source of energy especially for microorganisms. It is composed exclusively of Dglucose units that are linked by α-1,4- or α-1,6-glycosidic bonds. The two components of starch are amylose (15–25%), a linear polymer consisting of α-1,4-linked glucopyranose residues, and amylopec ...
... Starch is an important source of energy especially for microorganisms. It is composed exclusively of Dglucose units that are linked by α-1,4- or α-1,6-glycosidic bonds. The two components of starch are amylose (15–25%), a linear polymer consisting of α-1,4-linked glucopyranose residues, and amylopec ...
Nanosep® Centrifugal Devices - Protocols for Use
... A 500 µL sample of a 100 µg/mL solution of a 500 bp double-stranded DNA fragment was centrifuged in a 3K or 100K Nanosep device for 5 to 25 minutes. Recovered samples were quantitated using absorbance at 260 nm. Increased loss of the 500 bp fragment was seen at high speed for the 100K device. Remova ...
... A 500 µL sample of a 100 µg/mL solution of a 500 bp double-stranded DNA fragment was centrifuged in a 3K or 100K Nanosep device for 5 to 25 minutes. Recovered samples were quantitated using absorbance at 260 nm. Increased loss of the 500 bp fragment was seen at high speed for the 100K device. Remova ...
GeneMorph II EZClone Domain Mutagenesis Kit
... elements by introducing mutations during PCR. However, cloning amplicons generated by error-prone PCR can be difficult and labor-intensive due to low product yields, mutations at the ends which interfere with restriction-based cloning, and/or inefficient synthesis of 3´ dA overhangs or blunt ends wh ...
... elements by introducing mutations during PCR. However, cloning amplicons generated by error-prone PCR can be difficult and labor-intensive due to low product yields, mutations at the ends which interfere with restriction-based cloning, and/or inefficient synthesis of 3´ dA overhangs or blunt ends wh ...
Amino Acids, Proteins, and Enzymes
... RDA is 3 mg; deficiencies include night blindness, immune system repression, and slowed growth. Sources are meats and beta-carotenes in plants, yellow and green fruits and vegetables. H3C ...
... RDA is 3 mg; deficiencies include night blindness, immune system repression, and slowed growth. Sources are meats and beta-carotenes in plants, yellow and green fruits and vegetables. H3C ...
Amino Acids, Proteins, and Enzymes
... for the second half of the lab • When your group has agreed on the procedure share it with me for approval • Once it is approved everyone needs to write the procedure into their lab handout • All written procedures, observations, and questions need to be completed in your own words ...
... for the second half of the lab • When your group has agreed on the procedure share it with me for approval • Once it is approved everyone needs to write the procedure into their lab handout • All written procedures, observations, and questions need to be completed in your own words ...
Evolution of the enzymes of the citric acid cycle and the
... the JTT-F martix and starting from the NJ tree of ML distances. We often encountered distantly related genes encoding related protein families for different enzyme activities. These were usually included in the analysis if they helped to elucidate a general evolution pattern within a gene family, bu ...
... the JTT-F martix and starting from the NJ tree of ML distances. We often encountered distantly related genes encoding related protein families for different enzyme activities. These were usually included in the analysis if they helped to elucidate a general evolution pattern within a gene family, bu ...
Analysis of Toxoplasma gondii Repeat Region 529 bp (NCBI Acc
... Toxoplasma gondii repeat region was amplified using specific primers which was designed based on T. gondii repeat region 529 bp accessed from GeneBank (NCBI Acc No. AF 146527), as follows: forward primer 5'- GAC TCG GGC CCA GCT GCG -3’ and reverse primer 5'- CCT CTC CTA CCC CTC CTC -3'. Primers were ...
... Toxoplasma gondii repeat region was amplified using specific primers which was designed based on T. gondii repeat region 529 bp accessed from GeneBank (NCBI Acc No. AF 146527), as follows: forward primer 5'- GAC TCG GGC CCA GCT GCG -3’ and reverse primer 5'- CCT CTC CTA CCC CTC CTC -3'. Primers were ...
Recent advances in enzyme promiscuity | SpringerLink
... emergence of new enzyme. This hypothesis has been utilized for the construction of an efficient and thermostable phosphotriesterase from lactonase by creating simple double mutations His250Ile and Ile263Trp [37]. When complicated and challenging enzyme activities are sought by applying directed evol ...
... emergence of new enzyme. This hypothesis has been utilized for the construction of an efficient and thermostable phosphotriesterase from lactonase by creating simple double mutations His250Ile and Ile263Trp [37]. When complicated and challenging enzyme activities are sought by applying directed evol ...
Phenotypic and Genotypic Comparisons among Strains of the
... marine strains KB162, KB172, SH22, SH23, and SH31 were isolated from water or sediment in Norwegian lobster ponds. The marine isolates 37R and 88A were supposed to be avirulent strains of A . viridans (J. E. Stewart). Escherichia coli B (ATCC 11303) was used as a reference strain in the DNA studies. ...
... marine strains KB162, KB172, SH22, SH23, and SH31 were isolated from water or sediment in Norwegian lobster ponds. The marine isolates 37R and 88A were supposed to be avirulent strains of A . viridans (J. E. Stewart). Escherichia coli B (ATCC 11303) was used as a reference strain in the DNA studies. ...
Kinetics - University of San Diego Home Pages
... - One of the most powerful actions of enzymes. Group complementation - the ability to recognize specific regions of the substrate to align reactants with catalytic site. Based on non-covalent molecular interactions. Lock and key vs. induced fit - both occur. Induced fit takes place when binding of o ...
... - One of the most powerful actions of enzymes. Group complementation - the ability to recognize specific regions of the substrate to align reactants with catalytic site. Based on non-covalent molecular interactions. Lock and key vs. induced fit - both occur. Induced fit takes place when binding of o ...