Plant Functional Genomics
... munity have resulted in direct functional analysis of only about 1000 genes to date (5), this may seem like a tall order. However, it appears likely that the efficiency gained by “reverse genetics” will fundamentally change this equation. Large collections of insertion mutants are available for Arab ...
... munity have resulted in direct functional analysis of only about 1000 genes to date (5), this may seem like a tall order. However, it appears likely that the efficiency gained by “reverse genetics” will fundamentally change this equation. Large collections of insertion mutants are available for Arab ...
INF115 Compulsory Exercise 2 A genome is the term
... system must store which harbours a cruise visits on each day. It must be possible to find out which date a cruise arrives at and leaves a particular port. For every port the town name and telephone number of the port office should be stored. Every cruise ship has a number of cabins (rooms) in 4 t ...
... system must store which harbours a cruise visits on each day. It must be possible to find out which date a cruise arrives at and leaves a particular port. For every port the town name and telephone number of the port office should be stored. Every cruise ship has a number of cabins (rooms) in 4 t ...
ComPhy User Manual
... All sequence files need to be stored in directory “SeqDir” in “Input” folder. 2. All gene physical location files need to be in “GeneLocDir” in “Input” folder. The location file is in the format of following: Line 1: “genome_size #” shows genome sequence length, can not leave empty Line 2: “gene_num ...
... All sequence files need to be stored in directory “SeqDir” in “Input” folder. 2. All gene physical location files need to be in “GeneLocDir” in “Input” folder. The location file is in the format of following: Line 1: “genome_size #” shows genome sequence length, can not leave empty Line 2: “gene_num ...
JGI - MaizeGDB
... genic) regions, based on Morgante et al. results. Expect unique coverage of ~40% of B73 sequence. (alternative: MeF, C0t) – In a typical genic locus of 5 kb, conservatively expect ~100 mismatches or indels. Dense markers allows rapid development of multiple markers per gene. (Distribute via Gramene, ...
... genic) regions, based on Morgante et al. results. Expect unique coverage of ~40% of B73 sequence. (alternative: MeF, C0t) – In a typical genic locus of 5 kb, conservatively expect ~100 mismatches or indels. Dense markers allows rapid development of multiple markers per gene. (Distribute via Gramene, ...
Four types of evolution
... We need to be sure, then, that this really is the kind of change that organisms undergo over time: slow, gradual, random evolution towards forms of ever greater complexity. If that is not the story, then the choice presented may be a false one. We may find the desire to present it in such terms deep ...
... We need to be sure, then, that this really is the kind of change that organisms undergo over time: slow, gradual, random evolution towards forms of ever greater complexity. If that is not the story, then the choice presented may be a false one. We may find the desire to present it in such terms deep ...
CHAPTER 16 – THE MOLECULAR BASIS OF INHERITANCE
... About 44% of these sequences are transposable elements and related sequences. Eukaryotic transposable elements are two kinds: o Transposons – sequences that move around the genome by means of a DNA intermediate. These sequences can move by “cut-and-paste” mechanisms that remove them from one site of ...
... About 44% of these sequences are transposable elements and related sequences. Eukaryotic transposable elements are two kinds: o Transposons – sequences that move around the genome by means of a DNA intermediate. These sequences can move by “cut-and-paste” mechanisms that remove them from one site of ...
Schedule of Lecture and Laboratory Sessions
... Examine homologous recombination in a recipient, exconjugant cell Analyze the creation of knockout mice via homologous recombination and provide an example of a knockout mouse used as a disease model View aspects of plasmids used in transformation including ori, ampr, plasmid size, extrachromosomal ...
... Examine homologous recombination in a recipient, exconjugant cell Analyze the creation of knockout mice via homologous recombination and provide an example of a knockout mouse used as a disease model View aspects of plasmids used in transformation including ori, ampr, plasmid size, extrachromosomal ...
Colon Cancer Progression Tutorial
... several biological processes, including cell cycle, RNA processing and telomere maintenance. The arrow highlights the “telomerase-dependent telomere maintenance” ontology which is significantly over-represented in the upregulated gene list. Select the icon in the Genes column to view a list of the g ...
... several biological processes, including cell cycle, RNA processing and telomere maintenance. The arrow highlights the “telomerase-dependent telomere maintenance” ontology which is significantly over-represented in the upregulated gene list. Select the icon in the Genes column to view a list of the g ...
Genetics Option - Worked Examples
... Observed gene mutations are rare because DNA has the ability to repair itself. There are many genes which produce enzymes that repair damaged DNA: some have the ability to locate and cut out damaged sections of the DNA molecule, others add the missing bases, and still others repair the break in the ...
... Observed gene mutations are rare because DNA has the ability to repair itself. There are many genes which produce enzymes that repair damaged DNA: some have the ability to locate and cut out damaged sections of the DNA molecule, others add the missing bases, and still others repair the break in the ...
AP Bio Ch.18 “Genetics of Viruses and Bacteria” The Genetics of Viruses
... Euk have introns, prok don’t have introns and do not have RNA-splicing machinery. Use cDNA, includes only exons. Just add bacterial promoter and other control elements. 8. Describe two advantages of using yeast cells instead of bacteria as hosts for cloning or expressing eukaryotic genes. 1. They ar ...
... Euk have introns, prok don’t have introns and do not have RNA-splicing machinery. Use cDNA, includes only exons. Just add bacterial promoter and other control elements. 8. Describe two advantages of using yeast cells instead of bacteria as hosts for cloning or expressing eukaryotic genes. 1. They ar ...
BMC Biology - BioMedSearch
... transfer of a gene into the host from other microbial symbionts may have compensated for the deterioration of the gene in Buchnera, producing a selective advantage for the laterally transferred copy. Although symbiont-host lateral transfer is both surprising and exciting at first sight, retrospectiv ...
... transfer of a gene into the host from other microbial symbionts may have compensated for the deterioration of the gene in Buchnera, producing a selective advantage for the laterally transferred copy. Although symbiont-host lateral transfer is both surprising and exciting at first sight, retrospectiv ...
Lateral gene transfer between prokaryotes and multicellular
... transfer of a gene into the host from other microbial symbionts may have compensated for the deterioration of the gene in Buchnera, producing a selective advantage for the laterally transferred copy. Although symbiont-host lateral transfer is both surprising and exciting at first sight, retrospectiv ...
... transfer of a gene into the host from other microbial symbionts may have compensated for the deterioration of the gene in Buchnera, producing a selective advantage for the laterally transferred copy. Although symbiont-host lateral transfer is both surprising and exciting at first sight, retrospectiv ...
Introduction to Seed Development/Arabidopsis as a model organism
... Final HC70AL Symposium Spring, 2009 Page Three Some Things to Think About and Present in Your Individual Talks (you can add other data if relevant) 1. General Arabidopsis Gene Information a. What is the transcription factor gene? b. What other studies have been carried out on this gene? c. What is ...
... Final HC70AL Symposium Spring, 2009 Page Three Some Things to Think About and Present in Your Individual Talks (you can add other data if relevant) 1. General Arabidopsis Gene Information a. What is the transcription factor gene? b. What other studies have been carried out on this gene? c. What is ...
Slide 1
... • Almost all genes have mammalian homologs • Exaption of non-coding sequence is rare, especially for constitutively expressed exons • Conservation of exon-intron structure (number and phase of exons) • Conservation of length • Conservation of domains • Conservation of synteny ...
... • Almost all genes have mammalian homologs • Exaption of non-coding sequence is rare, especially for constitutively expressed exons • Conservation of exon-intron structure (number and phase of exons) • Conservation of length • Conservation of domains • Conservation of synteny ...
Document
... Genes may show sequence similarity that is limited to a certain region—some parts of a protein will be similar and other parts will be different. Genes may share similar motifs, meaning that they encode regions of similar amino acid sequence that aren't located right next to each other in the linear ...
... Genes may show sequence similarity that is limited to a certain region—some parts of a protein will be similar and other parts will be different. Genes may share similar motifs, meaning that they encode regions of similar amino acid sequence that aren't located right next to each other in the linear ...
Understanding Contemporary Genomics
... Therefore, if we insert this gene into this plant, the plant will poison insect pests. This argument is plausible, if a bit enthymematic. One premise that might start to ºesh it out is: If we insert a gene for x into a (living) genome then that genome will produce x. This premise shows us that the a ...
... Therefore, if we insert this gene into this plant, the plant will poison insect pests. This argument is plausible, if a bit enthymematic. One premise that might start to ºesh it out is: If we insert a gene for x into a (living) genome then that genome will produce x. This premise shows us that the a ...
Gene Structure: Searching Genbank and Interpreting
... /note="a in normal hbb; t in sickle cell anemia 78]" From the features table as shown above, nucleotide position number 62206 is mutated from a normal ‘a’ nucleotide to ‘t’ in the sickle cell anemia from. Normally the amino acid glutanic acid is in the beta chain of hemoglobin, in the mutant form it ...
... /note="a in normal hbb; t in sickle cell anemia 78]" From the features table as shown above, nucleotide position number 62206 is mutated from a normal ‘a’ nucleotide to ‘t’ in the sickle cell anemia from. Normally the amino acid glutanic acid is in the beta chain of hemoglobin, in the mutant form it ...
Symbiotic DNA in eukaryotic genomes
... evolutionary flexibility. Transposition in experimental popuBox 1. Transposons: cut-and-paste elements lations can increase the variability of metabolic and quantitative trait&l4 and consequently the rate of response to Transposons move by excision from a chromosome, followed by reinsertion into a n ...
... evolutionary flexibility. Transposition in experimental popuBox 1. Transposons: cut-and-paste elements lations can increase the variability of metabolic and quantitative trait&l4 and consequently the rate of response to Transposons move by excision from a chromosome, followed by reinsertion into a n ...
The Biotechnology Age: Issues and Impacts
... - Floral dip under vacuum -plant cells or seeds on growth media containing selection antibiotic (i.e. Kan). -Only engineered plants grow ...
... - Floral dip under vacuum -plant cells or seeds on growth media containing selection antibiotic (i.e. Kan). -Only engineered plants grow ...
When gene marriages don`t work out: divorce by subfunctionalization
... species (Populus trichocarpa and P. alba; [10–12]). Loss of RPL32 from chloroplast DNA occurred after Populus (order Malpighiales) diverged from other members of the Eurosid I clade such as cucumber (order Cucurbitales) and legumes (order Fabales). We identified database EST (expressed sequence tag) ...
... species (Populus trichocarpa and P. alba; [10–12]). Loss of RPL32 from chloroplast DNA occurred after Populus (order Malpighiales) diverged from other members of the Eurosid I clade such as cucumber (order Cucurbitales) and legumes (order Fabales). We identified database EST (expressed sequence tag) ...
DNA Chip Analysis and Bioinformatics
... Go to the National Center for Biotechnology Information (NCBI) site http://www.ncbi.nlm.nih.gov/ From the menu on the right click on “BLAST” (Basic Local Alignment Search Tool). Choose the BASIC BLAST program "Nucleotide Blast". Paste the probe DNA sequence into the query box, scroll down and select ...
... Go to the National Center for Biotechnology Information (NCBI) site http://www.ncbi.nlm.nih.gov/ From the menu on the right click on “BLAST” (Basic Local Alignment Search Tool). Choose the BASIC BLAST program "Nucleotide Blast". Paste the probe DNA sequence into the query box, scroll down and select ...
Pi kur, 2004
... specific phylogenetic hypotheses but also in precise reconstruction of the historical associations of all the taxa analysed. In other cases the amount of sequence information needed to resolve specific relationships will be dependent on the ...
... specific phylogenetic hypotheses but also in precise reconstruction of the historical associations of all the taxa analysed. In other cases the amount of sequence information needed to resolve specific relationships will be dependent on the ...
dsRNA synthesis RNAi (Howard Clarke)
... JFN RNAi Selection and preparation of DNA template: Chose an exon-rich region of genomic DNA 300bp in length (>500 is better, and 3’ UTR sequence is fine). Alternatively, cDNA clones or first-strand cDNA generated by RT-PCR can be used as template (see protocol “Oligo d(T) primed cDNA synthesis”). c ...
... JFN RNAi Selection and preparation of DNA template: Chose an exon-rich region of genomic DNA 300bp in length (>500 is better, and 3’ UTR sequence is fine). Alternatively, cDNA clones or first-strand cDNA generated by RT-PCR can be used as template (see protocol “Oligo d(T) primed cDNA synthesis”). c ...
Poliammine, evoluzione e patogenicità in Shigella spp
... expression of virulence genes residing on a large plasmid and on the chromosome. The genomes of Shigella and E. coli, its commensal ancenstor, are colinear and highly homologous. Critical events in the evolution of Shigella have been the acquisition of the virulence plasmid through lateral gene tran ...
... expression of virulence genes residing on a large plasmid and on the chromosome. The genomes of Shigella and E. coli, its commensal ancenstor, are colinear and highly homologous. Critical events in the evolution of Shigella have been the acquisition of the virulence plasmid through lateral gene tran ...
Practice exam (2010) key
... c) Because organelle genome transformation occurs by homologous recombination, mutant correction by homologous gene replacement is possible. Explain how you would select for a a chlamydomonas cell in which a mutant gene essential for chloroplast photosynthesis had been successfully replaced with a w ...
... c) Because organelle genome transformation occurs by homologous recombination, mutant correction by homologous gene replacement is possible. Explain how you would select for a a chlamydomonas cell in which a mutant gene essential for chloroplast photosynthesis had been successfully replaced with a w ...
Transposable element
A transposable element (TE or transposon) is a DNA sequence that can change its position within the genome, sometimes creating or reversing mutations and altering the cell's genome size. Transposition often results in duplication of the TE. Barbara McClintock's discovery of these jumping genes earned her a Nobel prize in 1983.TEs make up a large fraction of the C-value of eukaryotic cells. There are at least two classes of TEs: class I TEs generally function via reverse transcription, while class II TEs encode the protein transposase, which they require for insertion and excision, and some of these TEs also encode other proteins. It has been shown that TEs are important in genome function and evolution. In Oxytricha, which has a unique genetic system, they play a critical role in development. They are also very useful to researchers as a means to alter DNA inside a living organism.