presentation source
... Suppose you have identified a gene. What is its role in the biochemistry of its organism? Sequence databases can help us in formulating reasonable hypotheses. Search the database for genes with similar nucleotide sequences in other organisms. If the functions of the most similar genes are known ...
... Suppose you have identified a gene. What is its role in the biochemistry of its organism? Sequence databases can help us in formulating reasonable hypotheses. Search the database for genes with similar nucleotide sequences in other organisms. If the functions of the most similar genes are known ...
Lecture15
... • Comparisons of genes, proteins and non-coding sequences is not the only way to study relations between different species. • Attempts were made from 1930s to use chromosome rearrangements information for this purpose. • It has been shown that genomes consist of a relatively moderate number of “cons ...
... • Comparisons of genes, proteins and non-coding sequences is not the only way to study relations between different species. • Attempts were made from 1930s to use chromosome rearrangements information for this purpose. • It has been shown that genomes consist of a relatively moderate number of “cons ...
Polyploidy
... • In population-genetics terms, this is the switch from having four alleles at a single locus (tetrasomic inheritance) to having two alleles at each of two distinct loci (disomic ...
... • In population-genetics terms, this is the switch from having four alleles at a single locus (tetrasomic inheritance) to having two alleles at each of two distinct loci (disomic ...
J. Bacteriol.-2012-H
... and disseminated disease in both immunocompetent and immunocompromised individuals. NTM species previously considered nonpathogenic have now been shown to cause disease in humans. Mycobacterium vaccae, a rapidly growing and yellow-pigmented NTM, was first isolated, described, and named in 1962 (4). ...
... and disseminated disease in both immunocompetent and immunocompromised individuals. NTM species previously considered nonpathogenic have now been shown to cause disease in humans. Mycobacterium vaccae, a rapidly growing and yellow-pigmented NTM, was first isolated, described, and named in 1962 (4). ...
ppt - Sol Genomics Network
... - all available full-length tomato genes in GENBANK - TIGR full-length cDNA sequences (redundantly sequenced) - SGN unigene contigs with 5 or more ESTs - redundnacy correction 456 of 8,097 genes found in available genome sequence (5.6%) Correcting for 85% expectation yields 6.6% of target gene space ...
... - all available full-length tomato genes in GENBANK - TIGR full-length cDNA sequences (redundantly sequenced) - SGN unigene contigs with 5 or more ESTs - redundnacy correction 456 of 8,097 genes found in available genome sequence (5.6%) Correcting for 85% expectation yields 6.6% of target gene space ...
1) - life.illinois.edu
... between attDOT and attB by staggered cleavages seven base apart on each att site. The sites of cleavage in attDOT are shown between the D and D’ sites in the sequence. In vitro experiments indicated that the IntDOT integrase, which catalyzes the reaction, binds to two classes of sites in attDOT. One ...
... between attDOT and attB by staggered cleavages seven base apart on each att site. The sites of cleavage in attDOT are shown between the D and D’ sites in the sequence. In vitro experiments indicated that the IntDOT integrase, which catalyzes the reaction, binds to two classes of sites in attDOT. One ...
Text S1. Supporting Methods and Results METHODS
... the reference mouse C57BL/6 [2] contains 32,100 marked TSS (corresponding to 11,391 genes). Markings at typical liver genes were qualitatively very similar between our samples and the reference dataset. Of 3,990 liver genes from the UniProtKB Database that matched RefSeq genes, 74% were marked in po ...
... the reference mouse C57BL/6 [2] contains 32,100 marked TSS (corresponding to 11,391 genes). Markings at typical liver genes were qualitatively very similar between our samples and the reference dataset. Of 3,990 liver genes from the UniProtKB Database that matched RefSeq genes, 74% were marked in po ...
Alignment of pairs of sequences
... Why compare sequences? • To find whether two (or more) genes or proteins are evolutionarily related to each other • To find structurally or functionally similar regions within proteins ...
... Why compare sequences? • To find whether two (or more) genes or proteins are evolutionarily related to each other • To find structurally or functionally similar regions within proteins ...
Full-text PDF
... The cellular slime mold Dictyostelium discoideum has both multicellular and unicellular characteristics in its life cycle and hence it is considered as a powerful system for studying development, differenciation, and inter- or intra-cellular signaling. We have analyzed cDNA sequences of Dictyostelium ...
... The cellular slime mold Dictyostelium discoideum has both multicellular and unicellular characteristics in its life cycle and hence it is considered as a powerful system for studying development, differenciation, and inter- or intra-cellular signaling. We have analyzed cDNA sequences of Dictyostelium ...
Behind the Scenes of Gene Expression
... These so-called imprints have since been found in angiosperms, mammals, and some protozoa. Not until 1991, however, did researchers begin isolating a variety of genes whose expression depended on their parents of origin. That year, researchers identified two genes, Igf2r and H19, that are active onl ...
... These so-called imprints have since been found in angiosperms, mammals, and some protozoa. Not until 1991, however, did researchers begin isolating a variety of genes whose expression depended on their parents of origin. That year, researchers identified two genes, Igf2r and H19, that are active onl ...
Learning objectives
... 6. Describe the role of an expression vector. 7. Describe two advantages of using yeast cells instead of bacteria as hosts for cloning or expressing eukaryotic genes. 8. Describe the structure and function of a yeast artificial chromosome (YAC). 9. Describe two techniques to introduce recombinant DN ...
... 6. Describe the role of an expression vector. 7. Describe two advantages of using yeast cells instead of bacteria as hosts for cloning or expressing eukaryotic genes. 8. Describe the structure and function of a yeast artificial chromosome (YAC). 9. Describe two techniques to introduce recombinant DN ...
The Genetics of Viruses and Prokaryotes The Genetics of Viruses
... • The first to describe viruses was Beijerinck (1898), a Dutch microbial ecologist who showed that they were not killed by alcohol, did not grow on any media, and only reproduced inside a host. ...
... • The first to describe viruses was Beijerinck (1898), a Dutch microbial ecologist who showed that they were not killed by alcohol, did not grow on any media, and only reproduced inside a host. ...
The Humanized Fly - Barbara J. Culliton
... New genome studies have uncovered additional Drosophila genes that parallel genes for human neurological disease including Tay Sachs disease, which destroys the nervous systems of afflicted children before their third or fourth birthdays, and the gene "tau," which is thought to cause a kind of de ...
... New genome studies have uncovered additional Drosophila genes that parallel genes for human neurological disease including Tay Sachs disease, which destroys the nervous systems of afflicted children before their third or fourth birthdays, and the gene "tau," which is thought to cause a kind of de ...
Learning objectives
... 6. Describe the role of an expression vector. 7. Describe two advantages of using yeast cells instead of bacteria as hosts for cloning or expressing eukaryotic genes. 8. Describe the structure and function of a yeast artificial chromosome (YAC). 9. Describe two techniques to introduce recombinant DN ...
... 6. Describe the role of an expression vector. 7. Describe two advantages of using yeast cells instead of bacteria as hosts for cloning or expressing eukaryotic genes. 8. Describe the structure and function of a yeast artificial chromosome (YAC). 9. Describe two techniques to introduce recombinant DN ...
On Mapping the Human Genome
... have been identified (generally by analysis of family pedigrees), but only a small fraction have been actually located on the chromosomes. In one important sense, the job will never be complete, because even if there were a full genomic sequence, understanding how it relates to disease, normal funct ...
... have been identified (generally by analysis of family pedigrees), but only a small fraction have been actually located on the chromosomes. In one important sense, the job will never be complete, because even if there were a full genomic sequence, understanding how it relates to disease, normal funct ...
Lecture 13
... and augur well for the generic use of the technology. The silencing was much more profound with ihpRNA constructs than either anti-sense or co-suppression constructs; some ihpRNA transformants were close to exhibiting a complete knockout of the target endogenous gene. However, most of the ihpRNA pla ...
... and augur well for the generic use of the technology. The silencing was much more profound with ihpRNA constructs than either anti-sense or co-suppression constructs; some ihpRNA transformants were close to exhibiting a complete knockout of the target endogenous gene. However, most of the ihpRNA pla ...
Lecture 1 - Portal UniMAP
... Male semen possess the “vital heat” which cooks and shapes the menstrual blood which is the “physical substance’ to give rise to an offspring Embryo develops as a result of shaping power of vital heat. ...
... Male semen possess the “vital heat” which cooks and shapes the menstrual blood which is the “physical substance’ to give rise to an offspring Embryo develops as a result of shaping power of vital heat. ...
Genome Editing of a CArG Element in the Mouse Genome
... amount of time required to carry out all these steps is ≥1 year and probably ≈2 years; yet, despite all this effort, the end result was a mutant allele in which the 30-nucleotide CArG-bearing fragment was replaced with a 34-nucleotide loxP sequence, effective but crude. In contrast, Han et al1 were ...
... amount of time required to carry out all these steps is ≥1 year and probably ≈2 years; yet, despite all this effort, the end result was a mutant allele in which the 30-nucleotide CArG-bearing fragment was replaced with a 34-nucleotide loxP sequence, effective but crude. In contrast, Han et al1 were ...
A History of Genetics and Genomics
... following the rediscovery, other genetic principles such as linkage, lethal genes, and a bit later, maternal inheritance were described. In each case, the principles provided to be simple extensions of the Mendelian laws, providing further evidence of their importance. At the beginning of the centur ...
... following the rediscovery, other genetic principles such as linkage, lethal genes, and a bit later, maternal inheritance were described. In each case, the principles provided to be simple extensions of the Mendelian laws, providing further evidence of their importance. At the beginning of the centur ...
Lezione 23 - 24 martedì 10 maggio 2011
... This simple code between amino acids in TAL effectors and DNA bases in their target sites might be useful for protein engineering applications. Numerous groups have design artificial TAL effectors capable of recognizing new DNA sequences in a variety of experimental systems. Such engineered TAL effe ...
... This simple code between amino acids in TAL effectors and DNA bases in their target sites might be useful for protein engineering applications. Numerous groups have design artificial TAL effectors capable of recognizing new DNA sequences in a variety of experimental systems. Such engineered TAL effe ...
Introduction to Biology
... that contained all the polymerases and other enzymatic ingredients necessary for RNA transcription and translation. The synthetic virus was able to successfully replicate itself from this mixture.” ...
... that contained all the polymerases and other enzymatic ingredients necessary for RNA transcription and translation. The synthetic virus was able to successfully replicate itself from this mixture.” ...
Lecture_note_463BI
... 60 to 90 tRNA isoacceptors (Lin and Agris, 1980). The studies by McBride et al. (1989) as well as studies by others (see, e.g., 180620, 189930, 189920, 180640, 189880) indicated that tRNA genes and pseudogenes are dispersed on at least 7 human chromosomes and suggested that these sequences would pro ...
... 60 to 90 tRNA isoacceptors (Lin and Agris, 1980). The studies by McBride et al. (1989) as well as studies by others (see, e.g., 180620, 189930, 189920, 180640, 189880) indicated that tRNA genes and pseudogenes are dispersed on at least 7 human chromosomes and suggested that these sequences would pro ...
Expression of Genes Involved with Carotenoid Biosynthesis in
... are able to see that these genes are affected by Cefotax and Imipenem. These conditions can either inhibit the gene, or cause it to be expressed more. With the use of RAST, PATRIC, and BLAST, we are able to observe the genes and sequences associated in the pathway of carotenoid biosynthesis. With th ...
... are able to see that these genes are affected by Cefotax and Imipenem. These conditions can either inhibit the gene, or cause it to be expressed more. With the use of RAST, PATRIC, and BLAST, we are able to observe the genes and sequences associated in the pathway of carotenoid biosynthesis. With th ...
Gene duplication and rearrangement
... sample in different reading frames staggered by two bases ...
... sample in different reading frames staggered by two bases ...
Transposable element
A transposable element (TE or transposon) is a DNA sequence that can change its position within the genome, sometimes creating or reversing mutations and altering the cell's genome size. Transposition often results in duplication of the TE. Barbara McClintock's discovery of these jumping genes earned her a Nobel prize in 1983.TEs make up a large fraction of the C-value of eukaryotic cells. There are at least two classes of TEs: class I TEs generally function via reverse transcription, while class II TEs encode the protein transposase, which they require for insertion and excision, and some of these TEs also encode other proteins. It has been shown that TEs are important in genome function and evolution. In Oxytricha, which has a unique genetic system, they play a critical role in development. They are also very useful to researchers as a means to alter DNA inside a living organism.