The Human Genome Project
... What we’ve learned from our genome so far… • There are a relatively small number of human genes, less than 30,000, but they have a complex architecture that we are only beginning to understand and appreciate. -We know where 85% of genes are in the sequence. -We don’t know where the other 15% are be ...
... What we’ve learned from our genome so far… • There are a relatively small number of human genes, less than 30,000, but they have a complex architecture that we are only beginning to understand and appreciate. -We know where 85% of genes are in the sequence. -We don’t know where the other 15% are be ...
Can the process of advanced retinal degeneration
... The retinal pigment epithelium (RPE) is the outermost layer of the retina. It is made up of a single layer of pigmented cells, known as RPE cells. The RPE cells play a key role in normal retinal functioning, including several actions that assist, support and maintain the photoreceptors. Genetic muta ...
... The retinal pigment epithelium (RPE) is the outermost layer of the retina. It is made up of a single layer of pigmented cells, known as RPE cells. The RPE cells play a key role in normal retinal functioning, including several actions that assist, support and maintain the photoreceptors. Genetic muta ...
Is it ethical to use gene therapy to cure genetic
... A desired gene is selected and inserted into a genome to replace an “abnormal”, disease-causing gene. The genome is then transferred into a vector, most commonly an adenovirus, which will transport the gene into the patient. The vector is sent to a target cell in the patient’s genome and then “unloa ...
... A desired gene is selected and inserted into a genome to replace an “abnormal”, disease-causing gene. The genome is then transferred into a vector, most commonly an adenovirus, which will transport the gene into the patient. The vector is sent to a target cell in the patient’s genome and then “unloa ...
GTRC Declaration
... (c) must not code for a toxin with an LD50 of 100 g/kg or more, if the intention is to express the toxin at high levels; and (d) must not be uncharacterised nucleic acid from a toxin-producing organism; and (e) must not include a viral sequence, unless the donor nucleic acid: (i) is missing at leas ...
... (c) must not code for a toxin with an LD50 of 100 g/kg or more, if the intention is to express the toxin at high levels; and (d) must not be uncharacterised nucleic acid from a toxin-producing organism; and (e) must not include a viral sequence, unless the donor nucleic acid: (i) is missing at leas ...
Reproductive Technology
... genome into fragments – Only sequenced EST positive fragments – Used STSs to align sequences at the end – Updated their analysis from GenBank every ...
... genome into fragments – Only sequenced EST positive fragments – Used STSs to align sequences at the end – Updated their analysis from GenBank every ...
viruses - biology3u
... They must live as ___________ within the cells of a host organism They depend on their hosts for respiration, nutrition and all other functions of life to enable them to reproduce ...
... They must live as ___________ within the cells of a host organism They depend on their hosts for respiration, nutrition and all other functions of life to enable them to reproduce ...
Prokaryotic genomes
... and fragments with sizes between 1.6 and 2.0 kb purified from an agarose gel and ligated into a plasmid vector to produce a clone library. End sequences were obtained from clones taken from this library, and a computer used to identify overlaps between sequences. This resulted in 140 sequence contig ...
... and fragments with sizes between 1.6 and 2.0 kb purified from an agarose gel and ligated into a plasmid vector to produce a clone library. End sequences were obtained from clones taken from this library, and a computer used to identify overlaps between sequences. This resulted in 140 sequence contig ...
General Replication Strategies for RNA Viruses
... There are 4 human diseases classified as TSEs. These are Creudtzfeldt-Jacob disease (CJD), Gertsmann-Straussler syndrome (GSS), fatal familial insomnia (FFI) and kuru (9). The latter is confined to the Fore tribe of Papua New Guinea (PNG), and is caused by cannibalistic rituals, specifically the pre ...
... There are 4 human diseases classified as TSEs. These are Creudtzfeldt-Jacob disease (CJD), Gertsmann-Straussler syndrome (GSS), fatal familial insomnia (FFI) and kuru (9). The latter is confined to the Fore tribe of Papua New Guinea (PNG), and is caused by cannibalistic rituals, specifically the pre ...
Assembling and Annotating the Draft Human Genome
... – Lack of introns (but ~20% of real genes lack introns) – Not being the best place in genome an mRNA aligns (be careful not to filter out real paralogs) – Being inserted from another chromosome since dog/human common ancestor (breaking synteny). – High rate of mutation (Ka/Ks ratio). • Robert Baerts ...
... – Lack of introns (but ~20% of real genes lack introns) – Not being the best place in genome an mRNA aligns (be careful not to filter out real paralogs) – Being inserted from another chromosome since dog/human common ancestor (breaking synteny). – High rate of mutation (Ka/Ks ratio). • Robert Baerts ...
Introduction to self-assembly Self
... merits a detailed analysis since many gene therapy and bioengineering applications require delivering synthetic sequences via virion, and knowing the limitations on size and sequence would be helpful for developing new vectors. Key strategies of which you may be aware include producing a capsid from ...
... merits a detailed analysis since many gene therapy and bioengineering applications require delivering synthetic sequences via virion, and knowing the limitations on size and sequence would be helpful for developing new vectors. Key strategies of which you may be aware include producing a capsid from ...
Ebola lol
... Endothelial cells, mononuclear phagocytes, and hepatocytes are the main targets of infection. After infection, in a secreted glycoprotein (sGP) the Ebola virus glycoprotein (GP) is synthesized. Ebola replication overwhelms protein synthesis of infected cells and host immune defenses. The GP forms a ...
... Endothelial cells, mononuclear phagocytes, and hepatocytes are the main targets of infection. After infection, in a secreted glycoprotein (sGP) the Ebola virus glycoprotein (GP) is synthesized. Ebola replication overwhelms protein synthesis of infected cells and host immune defenses. The GP forms a ...
File
... vectors, for they have a number of advantages over other vectors, including the other two classes of vector for E. coli: plasmids and phage λ Filamentous bacteriophages have a number of unique properties that make them suitable as vectors ...
... vectors, for they have a number of advantages over other vectors, including the other two classes of vector for E. coli: plasmids and phage λ Filamentous bacteriophages have a number of unique properties that make them suitable as vectors ...
How do viruses differ?
... The International Committee on Taxonomy of Viruses (ICTV) developed the current classification system and put in place guidelines that put a greater weighting on certain virus properties in order to maintain family uniformity. A universal system for classifying viruses, and a unified taxonomy, has b ...
... The International Committee on Taxonomy of Viruses (ICTV) developed the current classification system and put in place guidelines that put a greater weighting on certain virus properties in order to maintain family uniformity. A universal system for classifying viruses, and a unified taxonomy, has b ...
REGISTRATION DOCUMENT FOR RECOMBINANT DNA RESEARCH
... Viral Vector .............. PLEASE ATTACH MAP(S) OF EXPRESSION CASSETTE. Adeno-associated virus (AAV) Adenovirus Lentivirus ...
... Viral Vector .............. PLEASE ATTACH MAP(S) OF EXPRESSION CASSETTE. Adeno-associated virus (AAV) Adenovirus Lentivirus ...
Respiratory-viruses-lecture-2005-no
... human and avian) • H3N2 1968 Hong Kong flu (reassortment) • H1N1 1977 Russian flu • H5N1 1997 Hong Kong (all genes avian) • H9N2 1999 Hong Kong (avian) • H5N1 2004 Vietnam(13) and Thialand(4) (avian) ...
... human and avian) • H3N2 1968 Hong Kong flu (reassortment) • H1N1 1977 Russian flu • H5N1 1997 Hong Kong (all genes avian) • H9N2 1999 Hong Kong (avian) • H5N1 2004 Vietnam(13) and Thialand(4) (avian) ...
doc
... C. In Xenopus laevis, nucleotide substitution patterns are consistent with the action of purifying selection on both copies of the duplicated genes D. For loci that have avoided nonfunctionalization in both duplicate copies, there is only a small amount of null alleles segregating in extant populati ...
... C. In Xenopus laevis, nucleotide substitution patterns are consistent with the action of purifying selection on both copies of the duplicated genes D. For loci that have avoided nonfunctionalization in both duplicate copies, there is only a small amount of null alleles segregating in extant populati ...
Filled in by Vector Core: Project: Received: Lot: BIOCENTER
... The materials produced by the BCK Virus Vector Laboratory require the following levels of containment: BSL1: plasmids and virus vectors tested free of replication competent virus (upon specific request) BSL2: virus vectors upon standard request if the inserted genetic material does not increase the ...
... The materials produced by the BCK Virus Vector Laboratory require the following levels of containment: BSL1: plasmids and virus vectors tested free of replication competent virus (upon specific request) BSL2: virus vectors upon standard request if the inserted genetic material does not increase the ...
Plant Viruses
... •Viral RNA consists of a 5'-end untranslated region of 68 nts. •Open reading frame at the 5’end codes for a 126 kDa protein and a readthrough of the UAG terminator signal by plant suppressor Tyr-tRNA to produce 183 kDa protein. •Genes downstream expressed via subgenomic RNAs. Genome expression strat ...
... •Viral RNA consists of a 5'-end untranslated region of 68 nts. •Open reading frame at the 5’end codes for a 126 kDa protein and a readthrough of the UAG terminator signal by plant suppressor Tyr-tRNA to produce 183 kDa protein. •Genes downstream expressed via subgenomic RNAs. Genome expression strat ...
Penn rDNA Registration Forms
... Your signature below indicates that you acknowledge all requirements and restrictions of the most current NIH guidelines for the Biosafety Level you have indicated above, unless modified by the IBC; that you accept responsibility for the safe conduct of the experiments conducted at this Biosafety Le ...
... Your signature below indicates that you acknowledge all requirements and restrictions of the most current NIH guidelines for the Biosafety Level you have indicated above, unless modified by the IBC; that you accept responsibility for the safe conduct of the experiments conducted at this Biosafety Le ...
Binary Vectors
... where the T-DNA region and the vir region are on separate plasmids. In the binary vector system, the two different plasmids employed are: • a wide-host-range small replicon, which has an origin of replication (ori) that permits the maintenance of the plasmid in a wide range of bacteria including E. ...
... where the T-DNA region and the vir region are on separate plasmids. In the binary vector system, the two different plasmids employed are: • a wide-host-range small replicon, which has an origin of replication (ori) that permits the maintenance of the plasmid in a wide range of bacteria including E. ...
Mammalian Expression Vectors Mammalian Transient Expression
... The cytomegalovirus (CMV) enhancer element plays a critical role in overcoming inefficient transcriptional activities of promoters, thereby enhancing transcription. The hCMV IE1 enhancer/promoter is one of the strongest enhancer/promoters known and is active in a wide range of cell types. (www.link. ...
... The cytomegalovirus (CMV) enhancer element plays a critical role in overcoming inefficient transcriptional activities of promoters, thereby enhancing transcription. The hCMV IE1 enhancer/promoter is one of the strongest enhancer/promoters known and is active in a wide range of cell types. (www.link. ...
Bats, camels and emerging viral disease by Dr. David L. (“Woody
... cases have occurred in Saudi Arabia, Jordan, Qatar and the United Arab Emirates, but infection has also been imported by sick travelers to Tunisia, the United Kingdom, Italy, Germany and France. To date, there are 130 laboratory-confirmed cases of infection with MERS; 58 patients have died. You may ...
... cases have occurred in Saudi Arabia, Jordan, Qatar and the United Arab Emirates, but infection has also been imported by sick travelers to Tunisia, the United Kingdom, Italy, Germany and France. To date, there are 130 laboratory-confirmed cases of infection with MERS; 58 patients have died. You may ...
Life Under a Microscope: Viruses Questions
... have a living cell to do its work. A virus attaches to a cell. It finds a way to get its genetic material into the cell. The virus's genetic material lets the virus take control of the cell. It turns the cell into a virus factory. The cell stops doing its normal work and begins making copies of the ...
... have a living cell to do its work. A virus attaches to a cell. It finds a way to get its genetic material into the cell. The virus's genetic material lets the virus take control of the cell. It turns the cell into a virus factory. The cell stops doing its normal work and begins making copies of the ...
CDC select agent survey form
... ____ 1. Select agent viral nucleic acids (synthetic or naturally derived, contiguous or fragmented, in host chromosomes or in expression vectors) that have potential to encode infectious and/or replication competent forms of any of the select agent viruses. ____ 2. Nucleic Acids (synthetic or natura ...
... ____ 1. Select agent viral nucleic acids (synthetic or naturally derived, contiguous or fragmented, in host chromosomes or in expression vectors) that have potential to encode infectious and/or replication competent forms of any of the select agent viruses. ____ 2. Nucleic Acids (synthetic or natura ...
REN Ee Chee
... We have developed a model for infecting liver cancer cells using a HBV replicon under the control of the native viral promoter. Using this system we have identified a host liver factor – hnRNP K – that regulates HBV replication. A naturally occurring base substitution (SNP) in the viral core promote ...
... We have developed a model for infecting liver cancer cells using a HBV replicon under the control of the native viral promoter. Using this system we have identified a host liver factor – hnRNP K – that regulates HBV replication. A naturally occurring base substitution (SNP) in the viral core promote ...
Adeno-associated virus
Adeno-associated virus (AAV) is a small virus which infects humans and some other primate species. AAV is not currently known to cause disease. The virus causes a very mild immune response, lending further support to its apparent lack of pathogenicity. Gene therapy vectors using AAV can infect both dividing and quiescent cells and persist in an extrachromosomal state without integrating into the genome of the host cell, although in the native virus some integration of virally carried genes into the host genome does occur. These features make AAV a very attractive candidate for creating viral vectors for gene therapy, and for the creation of isogenic human disease models. Recent human clinical trials using AAV for gene therapy in the retina have shown promise.AAV belongs to the genus Dependoparvovirus, which in turn belongs to the family Parvoviridae. The virus is a small (20 nm) replication-defective, nonenveloped virus.