pdf
... cloned genes in as many different bacteria as possible, and therefore should be endowed with a broad host-range promoter element. The well characterized lac promoter (Plac) of Escherichia coli has been shown to drive expression of cloned genes in a wide variety of Proteobacteria. Such an expression ...
... cloned genes in as many different bacteria as possible, and therefore should be endowed with a broad host-range promoter element. The well characterized lac promoter (Plac) of Escherichia coli has been shown to drive expression of cloned genes in a wide variety of Proteobacteria. Such an expression ...
PDF - European Journal of Biotechnology and Bioscience
... does not contain any viral genes and only the therapeutic gene, does not integrate into the genome. Instead the recombinant viral genome fuses at its ends via the ITR (inverted terminal repeats) recombination to form circular, episomal forms which are predicted to be the primary cause of the long te ...
... does not contain any viral genes and only the therapeutic gene, does not integrate into the genome. Instead the recombinant viral genome fuses at its ends via the ITR (inverted terminal repeats) recombination to form circular, episomal forms which are predicted to be the primary cause of the long te ...
shRNA FAQ - Functional Genomics Facility
... plate. Primary or other difficult-to-transduce cells may require more lentiviral supernatant. We suggest decreasing the number of cells plated to increase the multiplicity of infection (MOI) if necessary. Also, performing a limiting dilution titer on your cell line will determine the optimal amount ...
... plate. Primary or other difficult-to-transduce cells may require more lentiviral supernatant. We suggest decreasing the number of cells plated to increase the multiplicity of infection (MOI) if necessary. Also, performing a limiting dilution titer on your cell line will determine the optimal amount ...
53 Gene Targeting in Human Somatic Cells
... Rad51 is the key strand-exchange protein in homologous recombination.39 It is essential for the homology searches on the target DNA, i.e., the entire human genome (Figure 53–2, iv), that are required to localize the incoming DNA to its specific, cognate chromosomal counterpart.40 In humans, there ar ...
... Rad51 is the key strand-exchange protein in homologous recombination.39 It is essential for the homology searches on the target DNA, i.e., the entire human genome (Figure 53–2, iv), that are required to localize the incoming DNA to its specific, cognate chromosomal counterpart.40 In humans, there ar ...
the smallest dinoflagellate genome is yet to be found: a comment on
... current data suggests a possibility that dinoflagellate genomes may have increased as a result of extensive and repetitive duplication of individual genes, chromosomes, or even the whole genome (Beam and Himes 1984). Such extensive duplication can occur multiple times and can be followed by gene los ...
... current data suggests a possibility that dinoflagellate genomes may have increased as a result of extensive and repetitive duplication of individual genes, chromosomes, or even the whole genome (Beam and Himes 1984). Such extensive duplication can occur multiple times and can be followed by gene los ...
Deduced amino acid sequence of the fusion glycoprotein of turkey
... cDNA clones derived from oligo(dT)-selected infected cell RNA. The protein consists of 538 amino acids, the F2 and F1 subunits containing 102 (including the F 2 F1 connecting peptide RRRR) and 436 residues, respectively. Each subunit has one potential N-linked glycosylation site. The protein has 38 ...
... cDNA clones derived from oligo(dT)-selected infected cell RNA. The protein consists of 538 amino acids, the F2 and F1 subunits containing 102 (including the F 2 F1 connecting peptide RRRR) and 436 residues, respectively. Each subunit has one potential N-linked glycosylation site. The protein has 38 ...
Biosafety Manual - UC Berkeley, EHS
... This manual addresses the biological hazards frequently encountered in laboratories. Biological hazards include infectious or toxic microorganisms (including viral vectors), potentially infectious human substances, and research animals and their tissues, in cases from which transmission of infectiou ...
... This manual addresses the biological hazards frequently encountered in laboratories. Biological hazards include infectious or toxic microorganisms (including viral vectors), potentially infectious human substances, and research animals and their tissues, in cases from which transmission of infectiou ...
Use of Recombinant Adenovirus for Metabolic Engineering of
... DNA viruses have emerged as potentially attractive gene transfer vectors in recent years (Berkner, 1988;Graham and Prevec, 1991;Miller, 1992;Morgan and French Anderson, 1993; Nienhuis et al., 1993). Each virus under study has some distinct advantages and disadvantages. For example, vaccinia viruses ...
... DNA viruses have emerged as potentially attractive gene transfer vectors in recent years (Berkner, 1988;Graham and Prevec, 1991;Miller, 1992;Morgan and French Anderson, 1993; Nienhuis et al., 1993). Each virus under study has some distinct advantages and disadvantages. For example, vaccinia viruses ...
Teacher Guide: Vector Selector - Teach Genetics (Utah)
... Scientists refer to DNA delivery vehicles as vectors. Each vector is designed to target specific cells. Traditionally, vectors have been derived from viruses, including retroviruses, adenoviruses, adeno-associated viruses, and herpes simplex viruses. Components of the virus that cause disease are rem ...
... Scientists refer to DNA delivery vehicles as vectors. Each vector is designed to target specific cells. Traditionally, vectors have been derived from viruses, including retroviruses, adenoviruses, adeno-associated viruses, and herpes simplex viruses. Components of the virus that cause disease are rem ...
part 2b risk assessment of genetically modified human and animal
... Genetically Modified Organisms (Deliberate course of work, there are some types of Release) Regulations 1992 (as amended). experiment where particular caution should be However, if the gene therapy is a contained use taken. These are cases where the pathogenicity of then it is likely to fall into ri ...
... Genetically Modified Organisms (Deliberate course of work, there are some types of Release) Regulations 1992 (as amended). experiment where particular caution should be However, if the gene therapy is a contained use taken. These are cases where the pathogenicity of then it is likely to fall into ri ...
I = -[1/3*log 2 (1/3)+ 1/3*log 2 (1/3)+ 1/3*log 2 (1/3)] + 4.32 = 2.73
... Mutiple alignment of 3 protein sequences: ...
... Mutiple alignment of 3 protein sequences: ...
Slide 1 - Kenwood Academy High School
... Gene therapy is the practice of inserting functional genes into a person’s genome to replace faulty genes. Doctors and medical researchers are working to restore function to cells that are affected by genetic disorders. To accomplish this goal, scientists have to get the right gene to the right cell ...
... Gene therapy is the practice of inserting functional genes into a person’s genome to replace faulty genes. Doctors and medical researchers are working to restore function to cells that are affected by genetic disorders. To accomplish this goal, scientists have to get the right gene to the right cell ...
Identification of the equine herpesvirus type 1 glycoprotein 17/18 as
... the EHV-1 strain Abl gD homologue was obtained from both strands of the D N A by sequencing a 1602 bp Sinai to HindlII region from the Us region of the genome (GenBank accession number M60946). This sequence was almost identical to that obtained by Audonnet et al. (1990) for the Kentucky D (KyD) str ...
... the EHV-1 strain Abl gD homologue was obtained from both strands of the D N A by sequencing a 1602 bp Sinai to HindlII region from the Us region of the genome (GenBank accession number M60946). This sequence was almost identical to that obtained by Audonnet et al. (1990) for the Kentucky D (KyD) str ...
El Proyecto Genoma Humano
... • BioProject is an administrative object (defined by goal, target, funding, collaboration) • Genome is a biological object defining an organism at molecular level • Genome assembly is a complex data structure that defines the structure, relative position (scaffold) and chromosome placement of DNA se ...
... • BioProject is an administrative object (defined by goal, target, funding, collaboration) • Genome is a biological object defining an organism at molecular level • Genome assembly is a complex data structure that defines the structure, relative position (scaffold) and chromosome placement of DNA se ...
2011_InstructorSlidesR
... • Run 2-3 different projects, to keep group number to a manageable size and so different groups can present to and learn from each other • Select genes for study that have easily-identifiable differences in DNA sequence in the test population, so that comparisons can be made between test subjects • ...
... • Run 2-3 different projects, to keep group number to a manageable size and so different groups can present to and learn from each other • Select genes for study that have easily-identifiable differences in DNA sequence in the test population, so that comparisons can be made between test subjects • ...
Genome sequence analysis of Ebola virus in
... have been deployed alongside local workers at multiple treatment and diagnosis centres in Guinea, Sierra Leone and Liberia. Over 860 HCWs are known to have been infected [6]. Monitoring of the evolution of the viral genome during outbreaks is crucial for the early detection of mutations that may hav ...
... have been deployed alongside local workers at multiple treatment and diagnosis centres in Guinea, Sierra Leone and Liberia. Over 860 HCWs are known to have been infected [6]. Monitoring of the evolution of the viral genome during outbreaks is crucial for the early detection of mutations that may hav ...
Nucleotide sequences of the trailer, nucleocapsid protein gene and
... intermediate virulence and cause respiratory symptoms with low mortality, whereas velogenic strains are highly virulent and cause high mortality. The virus is a member of the genus Rubulavirus of the family Paramyxoviridae and contains a single-stranded negative-sense RNA genome (Murphy et al., 1995 ...
... intermediate virulence and cause respiratory symptoms with low mortality, whereas velogenic strains are highly virulent and cause high mortality. The virus is a member of the genus Rubulavirus of the family Paramyxoviridae and contains a single-stranded negative-sense RNA genome (Murphy et al., 1995 ...
The Mitochondria as a Minimal Chassis:
... rho+ mtDNA, the last S. douglasii cox1 exon and part of its terminator region were cloned upstream of the cox1::RIP1m gene. This large additional region homologous to the 3′ part of the cox1 gene (886 bp) should promote integration of RIP1m between the cox1 and atp8 genes in rho+ mtDNA (Fig. 1C). S. ...
... rho+ mtDNA, the last S. douglasii cox1 exon and part of its terminator region were cloned upstream of the cox1::RIP1m gene. This large additional region homologous to the 3′ part of the cox1 gene (886 bp) should promote integration of RIP1m between the cox1 and atp8 genes in rho+ mtDNA (Fig. 1C). S. ...
Efficient expression of ribozyme and reduction of stromelysin mRNA
... Figure 2: Schematic Representation of rAAV Vectors. Recombinant viruses contain the ribozyme (Rz) transcription units as noted. vAT22 is the "empty" (contains no ribozyme transcription unit) control virus derived from the backbone plasmid pAT22. AAV-ITR, AAV inverted terminal repeat sequence; ∆ - La ...
... Figure 2: Schematic Representation of rAAV Vectors. Recombinant viruses contain the ribozyme (Rz) transcription units as noted. vAT22 is the "empty" (contains no ribozyme transcription unit) control virus derived from the backbone plasmid pAT22. AAV-ITR, AAV inverted terminal repeat sequence; ∆ - La ...
HIV Drug Resistance Assessment and Prevention Strategy
... headaches. • While the symptoms resembled dengue fever, testing ruled out this and several other potential causes. • Then, in May 2015, researchers in Brazil determined, using the RT-PCR technique, that the illness was an outbreak of Zika virus. ...
... headaches. • While the symptoms resembled dengue fever, testing ruled out this and several other potential causes. • Then, in May 2015, researchers in Brazil determined, using the RT-PCR technique, that the illness was an outbreak of Zika virus. ...
Notes
... Suffix tree Suffix tree is data structure, which allows one to find, extremely efficiently, all distinct subsequences in a given sequence. There are efficient algorithms to construct suffix trees given by Weiner (1973) and McCreight (1976) (in linear time) For the task of comparing two DNA se ...
... Suffix tree Suffix tree is data structure, which allows one to find, extremely efficiently, all distinct subsequences in a given sequence. There are efficient algorithms to construct suffix trees given by Weiner (1973) and McCreight (1976) (in linear time) For the task of comparing two DNA se ...
Ch 18
... The story of how viruses were discovered begins in 1883 with research on the cause of tobacco mosaic disease by Adolf Mayer. This disease stunts tobacco plant growth and mottles plant leaves. Mayer concluded that the disease was infectious when he found that he could transmit the disease by rubb ...
... The story of how viruses were discovered begins in 1883 with research on the cause of tobacco mosaic disease by Adolf Mayer. This disease stunts tobacco plant growth and mottles plant leaves. Mayer concluded that the disease was infectious when he found that he could transmit the disease by rubb ...
Viruses, Koalas and Leukaemia - Australian Wildlife Rehabilitation
... equated with disease. What they did when not causing disease and how they fitted into the planets ecology remained a black box. Only in the last 15 years have investigators started to look more broadly at virus ecology and are now realizing that while viruses sometimes cause disease they also underp ...
... equated with disease. What they did when not causing disease and how they fitted into the planets ecology remained a black box. Only in the last 15 years have investigators started to look more broadly at virus ecology and are now realizing that while viruses sometimes cause disease they also underp ...
Adeno-associated virus
Adeno-associated virus (AAV) is a small virus which infects humans and some other primate species. AAV is not currently known to cause disease. The virus causes a very mild immune response, lending further support to its apparent lack of pathogenicity. Gene therapy vectors using AAV can infect both dividing and quiescent cells and persist in an extrachromosomal state without integrating into the genome of the host cell, although in the native virus some integration of virally carried genes into the host genome does occur. These features make AAV a very attractive candidate for creating viral vectors for gene therapy, and for the creation of isogenic human disease models. Recent human clinical trials using AAV for gene therapy in the retina have shown promise.AAV belongs to the genus Dependoparvovirus, which in turn belongs to the family Parvoviridae. The virus is a small (20 nm) replication-defective, nonenveloped virus.