Influenza A virus
... completely new surface proteins are acquired by the virus. This process occurs when viruses with segmented genomes exchange segments after coinfection. The new reassortant viruses display dramatic changes in surface proteins that facilitate escape from immune surveillance. ...
... completely new surface proteins are acquired by the virus. This process occurs when viruses with segmented genomes exchange segments after coinfection. The new reassortant viruses display dramatic changes in surface proteins that facilitate escape from immune surveillance. ...
Complete Genome Sequence of Bacillus thuringiensis Strain 407 Cry-
... acillus thuringiensis is a Gram-positive bacterium that is pathogenic towards a range of insect and nematode species. This is largely mediated through the production of crystal (cry) toxin proteins, which vary among B. thuringiensis strains and enable the infection of particular hosts. Because of th ...
... acillus thuringiensis is a Gram-positive bacterium that is pathogenic towards a range of insect and nematode species. This is largely mediated through the production of crystal (cry) toxin proteins, which vary among B. thuringiensis strains and enable the infection of particular hosts. Because of th ...
UCSC genome support forum
... content. The tiles that Galt referred to are 11-base sequences that are overrepresented in the genome. In addition, parts of the genome that are annotated by RepeatMasker or short Tandem Repeats are soft-masked so that alignments cannot begin there -- but alignments can extend through those tiles an ...
... content. The tiles that Galt referred to are 11-base sequences that are overrepresented in the genome. In addition, parts of the genome that are annotated by RepeatMasker or short Tandem Repeats are soft-masked so that alignments cannot begin there -- but alignments can extend through those tiles an ...
GAlibLecture
... Overlapping and non-overlapping are supported Customizable chromosome types, mutation, objective , and crossover functions. • Well written documentation and manuals ...
... Overlapping and non-overlapping are supported Customizable chromosome types, mutation, objective , and crossover functions. • Well written documentation and manuals ...
Mapping strategies for sequence reads (with focus on RNA-seq)
... ted by its splice site scoring algorithm; its authors mapping 71 million RNA-Seq reads to A.thaliana 0 CPU hours, which is ∼180 000 reads per CPU hour. ...
... ted by its splice site scoring algorithm; its authors mapping 71 million RNA-Seq reads to A.thaliana 0 CPU hours, which is ∼180 000 reads per CPU hour. ...
- Global Genes
... in the cell’s membranes, including the membrane of the nucleus, allowing the DNA to get in. 1. Naked DNA • This DNA most often takes the form of a small circle and is called a plasmid. • Bacteria use plasmids to exchange DNA, so it also is a method borrowed from nature by researchers. • A plasmid ...
... in the cell’s membranes, including the membrane of the nucleus, allowing the DNA to get in. 1. Naked DNA • This DNA most often takes the form of a small circle and is called a plasmid. • Bacteria use plasmids to exchange DNA, so it also is a method borrowed from nature by researchers. • A plasmid ...
Nucleic acid hybridisation and polymerase chain reaction
... between probe and target. Several reports indicate that when highly-conserved regions of the genome are selected and hybridisation conditions are optimised, oligonucleotide probes can b e useful for viral detection (9, 10, 30). R N A p r o b e s , which are m o r e difficult to handle because of the ...
... between probe and target. Several reports indicate that when highly-conserved regions of the genome are selected and hybridisation conditions are optimised, oligonucleotide probes can b e useful for viral detection (9, 10, 30). R N A p r o b e s , which are m o r e difficult to handle because of the ...
Articles - American Scientist
... in more detail in the 3D model on the cover of this magazine. The stargate substructure of genetic material and protein coat) viruses. This unprecedented property lacks fibers and is visible as an indentation has an icosahedral core of ~500 nano- and other features of its lifestyle have in the fiber ...
... in more detail in the 3D model on the cover of this magazine. The stargate substructure of genetic material and protein coat) viruses. This unprecedented property lacks fibers and is visible as an indentation has an icosahedral core of ~500 nano- and other features of its lifestyle have in the fiber ...
BioinformaIcs Journal Club
... Where do these sequences come from? • reads from the same biotope were pooled • unique sequences removed • assambled contjointly by biotype, using the Assambler ABySS ⇒ Overall, 94 Mb of conBg sequences, ...
... Where do these sequences come from? • reads from the same biotope were pooled • unique sequences removed • assambled contjointly by biotype, using the Assambler ABySS ⇒ Overall, 94 Mb of conBg sequences, ...
Document
... Schematic representation of the ruvAB operon. The position of the ruvAB promoter is indicated by a bent arrow and that of the putative transcription terminator by a loop. The initiation codons of ruvA and ruvB are indicated (ATG). The vertical lines below show the position of the 10 MudX insertions ...
... Schematic representation of the ruvAB operon. The position of the ruvAB promoter is indicated by a bent arrow and that of the putative transcription terminator by a loop. The initiation codons of ruvA and ruvB are indicated (ATG). The vertical lines below show the position of the 10 MudX insertions ...
Document
... The assignment of a function to a gene product can be made by a human curator by assessing all of the data (similarities, protein domains, signal peptide etc) This is a labour intensive process and like gene prediction is subjective ...
... The assignment of a function to a gene product can be made by a human curator by assessing all of the data (similarities, protein domains, signal peptide etc) This is a labour intensive process and like gene prediction is subjective ...
bbr038online 474..484 - Oxford Academic
... genome sequencing based on new technologies will yield more genome sequences that are not well assembled [2, 3], which in turn will hinder comprehensive annotation of the protein-coding landscape. In particular, a major challenge is the identification of protein-coding sequences split into multiple ...
... genome sequencing based on new technologies will yield more genome sequences that are not well assembled [2, 3], which in turn will hinder comprehensive annotation of the protein-coding landscape. In particular, a major challenge is the identification of protein-coding sequences split into multiple ...
List of DNIRs - UNSW Research Gateway
... A dealing of any of the following kinds, or involving a dealing of the following kinds, is not a notifiable low risk dealing: (a) a dealing (other than a dealing mentioned in paragraph 2.1 (h)) involving cloning of nucleic acid encoding a toxin having an LD50 of less than 100 g/kg; (b) a dealing in ...
... A dealing of any of the following kinds, or involving a dealing of the following kinds, is not a notifiable low risk dealing: (a) a dealing (other than a dealing mentioned in paragraph 2.1 (h)) involving cloning of nucleic acid encoding a toxin having an LD50 of less than 100 g/kg; (b) a dealing in ...
Review Article Viral Bacterial Artificial - diss.fu
... mini-F insertion site is limited to the location of the unique restriction site. Insertion into open reading frames (ORFs) or promoters of the virus genome can impair or abrogate infectivity of BAC-derived viruses. Last but not least, ligation and transformation procedures for large BAC vectors are ...
... mini-F insertion site is limited to the location of the unique restriction site. Insertion into open reading frames (ORFs) or promoters of the virus genome can impair or abrogate infectivity of BAC-derived viruses. Last but not least, ligation and transformation procedures for large BAC vectors are ...
Performance of AccuPlex rEbola GP/NP Reference
... technology. The use of recombinant virus, containing target sequences from Ebola virus has many advantages as a NAT quality control material. First, it mimics clinical samples because it undergoes the entire extraction procedure. Second, the product is non-infectious and ensures biological safety fo ...
... technology. The use of recombinant virus, containing target sequences from Ebola virus has many advantages as a NAT quality control material. First, it mimics clinical samples because it undergoes the entire extraction procedure. Second, the product is non-infectious and ensures biological safety fo ...
CHAPTER 1 LITERATURE SURVEY
... an infectious genome RNP. The same strategy was also used for the successful recovery and manipulation of paramyxoviruses (Collins et al. 1995) and of the prototype rhabdovirus VSV (Lawson et al. 1995; Whelan et al. 1995). In the latter case, this reverse genetics system was utilised in a fascinatin ...
... an infectious genome RNP. The same strategy was also used for the successful recovery and manipulation of paramyxoviruses (Collins et al. 1995) and of the prototype rhabdovirus VSV (Lawson et al. 1995; Whelan et al. 1995). In the latter case, this reverse genetics system was utilised in a fascinatin ...
ACEMBL System:
... promoters T7 and Lac, as well as the T7 terminator element (Illustr.1, 10). The T7 system is currently most commonly used; it requires bacterial strains which contain a T7 polymerase gene in the E. coli genome. The Lac promoter is a strong endogenous promoter which can be utilized in most strains. A ...
... promoters T7 and Lac, as well as the T7 terminator element (Illustr.1, 10). The T7 system is currently most commonly used; it requires bacterial strains which contain a T7 polymerase gene in the E. coli genome. The Lac promoter is a strong endogenous promoter which can be utilized in most strains. A ...
Possible consequences of the overlap between the CaMV 35S
... Cauliflower mosaic virus (CaMV) is a pararetrovirus (Caulimoviridae) that infects members of the Brassicaceae family. CaMV was one of first plant DNA viruses to be studied, and its double-stranded circular DNA genome [~8 kg base (kb)] has been completely sequenced.1 The genome encodes seven genes an ...
... Cauliflower mosaic virus (CaMV) is a pararetrovirus (Caulimoviridae) that infects members of the Brassicaceae family. CaMV was one of first plant DNA viruses to be studied, and its double-stranded circular DNA genome [~8 kg base (kb)] has been completely sequenced.1 The genome encodes seven genes an ...
Spontaneous Mutation Rate of Measles Virus: Direct Estimation
... per site per replication (10). The average estimate that we obtained for measles virus, 1.2 3 1024 per replication, falls in this range. This mutation rate, however, represents a composite of the rates of all mutations that conferred resistance to MAb 80-III-B2. Genetic characterization of five isol ...
... per site per replication (10). The average estimate that we obtained for measles virus, 1.2 3 1024 per replication, falls in this range. This mutation rate, however, represents a composite of the rates of all mutations that conferred resistance to MAb 80-III-B2. Genetic characterization of five isol ...
Determining the epitope dominance on the capsid of a SAT2 fmdv by mutational analysis, P.A.Opperman
... Opperman, P.A., Theron, J. and F.F. Maree ...
... Opperman, P.A., Theron, J. and F.F. Maree ...
Sequences of flavivirus-related RNA viruses persist in DNA form
... et al. (1997) described the presence of LCMV sequences present as DNA in mice over 200 days after infection. In vitro studies demonstrated that DNA synthesis from viral RNA was mediated by endogenous reverse transcriptase activity, but the possibility of integration of the viral sequence into the ce ...
... et al. (1997) described the presence of LCMV sequences present as DNA in mice over 200 days after infection. In vitro studies demonstrated that DNA synthesis from viral RNA was mediated by endogenous reverse transcriptase activity, but the possibility of integration of the viral sequence into the ce ...
Molecular pathogenesis of feline leukemia virus
... Fig. 1. Concepts of retroviral insertional mutagenesis. The provirus contains long terminal repeats (LTRs) at both ends. LTRs can be subdivided into three regions: U3, R and U5. U3 contains the enhancer and promoter sequences that drive viral as well as cellular gene transcription. Retroviral gag, p ...
... Fig. 1. Concepts of retroviral insertional mutagenesis. The provirus contains long terminal repeats (LTRs) at both ends. LTRs can be subdivided into three regions: U3, R and U5. U3 contains the enhancer and promoter sequences that drive viral as well as cellular gene transcription. Retroviral gag, p ...
Virus induced gene silencing, a post transcriptional gene silencing
... Therefore, a number of viral-derived vectors have been developed (Table 1) and many procedures have been optimized by the researchers. TRV system was efficiently optimized for efficient silencing of Solanaceous plants [14; 15], and the system was also applied for tomato to study role of fruit ripeni ...
... Therefore, a number of viral-derived vectors have been developed (Table 1) and many procedures have been optimized by the researchers. TRV system was efficiently optimized for efficient silencing of Solanaceous plants [14; 15], and the system was also applied for tomato to study role of fruit ripeni ...
Adeno-associated virus
Adeno-associated virus (AAV) is a small virus which infects humans and some other primate species. AAV is not currently known to cause disease. The virus causes a very mild immune response, lending further support to its apparent lack of pathogenicity. Gene therapy vectors using AAV can infect both dividing and quiescent cells and persist in an extrachromosomal state without integrating into the genome of the host cell, although in the native virus some integration of virally carried genes into the host genome does occur. These features make AAV a very attractive candidate for creating viral vectors for gene therapy, and for the creation of isogenic human disease models. Recent human clinical trials using AAV for gene therapy in the retina have shown promise.AAV belongs to the genus Dependoparvovirus, which in turn belongs to the family Parvoviridae. The virus is a small (20 nm) replication-defective, nonenveloped virus.