The Engineered Chloroplast Genome Just Got Smarter
... Long-term delivery of several protein drugs by injection can lead to adverse effects. One such complication is the development of antibodies to injected proteins, Thereby neutralizing the effects of injected drug or, in some cases, leading to toxic antibodies (such as immunoglobulin IgE) that ...
... Long-term delivery of several protein drugs by injection can lead to adverse effects. One such complication is the development of antibodies to injected proteins, Thereby neutralizing the effects of injected drug or, in some cases, leading to toxic antibodies (such as immunoglobulin IgE) that ...
Types of variation in DNA-A among isolates of East African cassava
... EACMV-MK, 2804 nt) were compared with the partial sequence of EACMV-YG (Hong et al., 1993 ; AJ006461), and the published sequences of DNA-A of EACMV-T (2801 nt ; Z83256), UgV-C39 (2799 nt ; Z83257), ACMV-K (2779 nt ; J02057) and TYLCV-Is (2787 nt ; X15656). In length and percentage sequence identity ...
... EACMV-MK, 2804 nt) were compared with the partial sequence of EACMV-YG (Hong et al., 1993 ; AJ006461), and the published sequences of DNA-A of EACMV-T (2801 nt ; Z83256), UgV-C39 (2799 nt ; Z83257), ACMV-K (2779 nt ; J02057) and TYLCV-Is (2787 nt ; X15656). In length and percentage sequence identity ...
CapeTownGenomes
... Nebulized or adapter-ligated DNA fragments are attached to beads PCR amplification step Each DNA-bound bead is placed into picotiterplate where the DNA synthesis will take place Measure incorporation of a nucleotide using the light produced via the luciferase enzyme (nucleotide incorporation release ...
... Nebulized or adapter-ligated DNA fragments are attached to beads PCR amplification step Each DNA-bound bead is placed into picotiterplate where the DNA synthesis will take place Measure incorporation of a nucleotide using the light produced via the luciferase enzyme (nucleotide incorporation release ...
Curr. Opin. Virol. v2-SFE140709
... ESS, two viruses involved in this game will coexist in the infected plant for long periods of time. The second most commonly played game is HD. According to this game, both viruses compete for a limiting resource. If one competitor is better using a ...
... ESS, two viruses involved in this game will coexist in the infected plant for long periods of time. The second most commonly played game is HD. According to this game, both viruses compete for a limiting resource. If one competitor is better using a ...
RESEARCH ARTICLES
... Fig. 2. Analysis of the assembly intermediates. (A) Not I and Sbf I double restriction digestion analysis of assembly 341350 purified from E. coli. These restriction enzymes release the vector fragments (5.5 and 3.4 kb) from the 10-kb insert. Insert DNA was separated from the vector DNA on a 0.8% E ...
... Fig. 2. Analysis of the assembly intermediates. (A) Not I and Sbf I double restriction digestion analysis of assembly 341350 purified from E. coli. These restriction enzymes release the vector fragments (5.5 and 3.4 kb) from the 10-kb insert. Insert DNA was separated from the vector DNA on a 0.8% E ...
Conformationally Complex Epitope on Glycoprotein H
... Thirteen antigenic variants of herpes simplex virus which were resistant to neutralization by monoclonal antibody 52S or LP11 were isolated and characterized. The antibodies in the absence of complement potently neutralize infectivity of wild-type virus as well as inhibit the transfer of virus from ...
... Thirteen antigenic variants of herpes simplex virus which were resistant to neutralization by monoclonal antibody 52S or LP11 were isolated and characterized. The antibodies in the absence of complement potently neutralize infectivity of wild-type virus as well as inhibit the transfer of virus from ...
Nucleotide sequences from tomato leaf curl viruses from different
... these differences, the 34 nucleotide sequence G C G G C C A T c C G T a T A A T A T T A C C G G A T G G C C G C G , which can form the well-known stem-loop structure, was the same in all five viruses except that the lower case ' c ' is T in T L C V - I N and the ' a ' is C in TLCV-A. This sequence, ...
... these differences, the 34 nucleotide sequence G C G G C C A T c C G T a T A A T A T T A C C G G A T G G C C G C G , which can form the well-known stem-loop structure, was the same in all five viruses except that the lower case ' c ' is T in T L C V - I N and the ' a ' is C in TLCV-A. This sequence, ...
the genome of herpes simplex virus: structure, replication and
... cis-acting signals fo r D N A replication a n d processing There was some early evidence, from electron microscopic analyses of H SV D N A extracted from infected cells, that the virus genome might possess one or more specific sites at which replicative synthesis of D N A started (Friedmann et al. 1 ...
... cis-acting signals fo r D N A replication a n d processing There was some early evidence, from electron microscopic analyses of H SV D N A extracted from infected cells, that the virus genome might possess one or more specific sites at which replicative synthesis of D N A started (Friedmann et al. 1 ...
Retrovirus Integration Database (RID): a public database for
... The NCI Retrovirus Integration Database is a MySql-based relational database created for storing and retrieving comprehensive information about retroviral integration sites, primarily, but not exclusively, HIV-1. The database is accessible to the public for submission or extraction of data originati ...
... The NCI Retrovirus Integration Database is a MySql-based relational database created for storing and retrieving comprehensive information about retroviral integration sites, primarily, but not exclusively, HIV-1. The database is accessible to the public for submission or extraction of data originati ...
Molecular evolution of swine vesicular disease virus
... Division of Molecular Biology, Institute for Animal Health, Compton Laboratory, Compton, Newbury RG20 7NN, UK ...
... Division of Molecular Biology, Institute for Animal Health, Compton Laboratory, Compton, Newbury RG20 7NN, UK ...
Plant Virology
... • Tomato bushy stunt virus is typical, wellstudied example • Each particle contains only a single molecule of RNA (4800 nt) and T= 3 Lattice 180 copies of the coat C protein subunit (387 aa; 41 kd) • Viruses similar to TBSV will self-assemble in N vitro from protein subunits + nucleic acid Protein S ...
... • Tomato bushy stunt virus is typical, wellstudied example • Each particle contains only a single molecule of RNA (4800 nt) and T= 3 Lattice 180 copies of the coat C protein subunit (387 aa; 41 kd) • Viruses similar to TBSV will self-assemble in N vitro from protein subunits + nucleic acid Protein S ...
ucsc genome research primer - Center for Biomolecular Science
... Bioinformatics Group works to make the human genome sequence even more useful for science and medicine by identifying and annotating its key functional elements in such a way that they are easily accessible to researchers. This process of discovery and categorization is a critical step toward fully ...
... Bioinformatics Group works to make the human genome sequence even more useful for science and medicine by identifying and annotating its key functional elements in such a way that they are easily accessible to researchers. This process of discovery and categorization is a critical step toward fully ...
repetitive extragenic palindromic sequences in pseudomonas
... following negatively oriented REP sequences shared another sequence Considering the conserved palindromy of REP sequences and the conserved arrangement of the clusters it is probable that REP sequences adopt conformations with peculiar secondary structures especially suitable to be specifically reco ...
... following negatively oriented REP sequences shared another sequence Considering the conserved palindromy of REP sequences and the conserved arrangement of the clusters it is probable that REP sequences adopt conformations with peculiar secondary structures especially suitable to be specifically reco ...
Genome browsers and other resources
... The 2015 issue is 1274 pages long and has 172 manuscripts in 8 categories 1. Nucleic acid sequence, structure and regulation 2. Protein sequence and structure, motifs and domains 3. Metabolic and signaling pathways, enzymes 4. Viruses, bacteria, protozoa and fungi ...
... The 2015 issue is 1274 pages long and has 172 manuscripts in 8 categories 1. Nucleic acid sequence, structure and regulation 2. Protein sequence and structure, motifs and domains 3. Metabolic and signaling pathways, enzymes 4. Viruses, bacteria, protozoa and fungi ...
Antisense derivatives of U7 small nuclear RNA as
... [7]. In this case, the U7 snRNP may have acted by masking exonic splicing enhancer (ESE) sequences or by affecting the flexibility of the exon. In our hands, a U7 Sm OPT derivative could induce skipping of exon 7 in the human survival of motoneuron 1 (SMN1) and SMN2 genes, even if it did not target ...
... [7]. In this case, the U7 snRNP may have acted by masking exonic splicing enhancer (ESE) sequences or by affecting the flexibility of the exon. In our hands, a U7 Sm OPT derivative could induce skipping of exon 7 in the human survival of motoneuron 1 (SMN1) and SMN2 genes, even if it did not target ...
Functional expression of lepidopteran
... genera Heliothis, Spodoptera and Trichoplusia [1]. However, the use of baculoviruses as insect control agents has met with limited success due to lack of field persistence and slower speed of action on insect mortality when compared to classical chemical insecticides [10]. In order to enhance the ef ...
... genera Heliothis, Spodoptera and Trichoplusia [1]. However, the use of baculoviruses as insect control agents has met with limited success due to lack of field persistence and slower speed of action on insect mortality when compared to classical chemical insecticides [10]. In order to enhance the ef ...
Antisense derivatives of U7 small nuclear RNA as
... mutagenesis with lentiviral vectors are still justified [22], this risk is minimal for AAV vectors because of their non-insertional behaviour. Furthermore, U snRNA promoters are not known to act as enhancers on nearby genes, which may translate into a lower risk of insertional mutagenesis if the rem ...
... mutagenesis with lentiviral vectors are still justified [22], this risk is minimal for AAV vectors because of their non-insertional behaviour. Furthermore, U snRNA promoters are not known to act as enhancers on nearby genes, which may translate into a lower risk of insertional mutagenesis if the rem ...
When Did Virology Start? - American Society for Microbiology
... could it live in harmony with its bacterial host, he noted. And, by some process of induction (stimulus), the prophage again became an infectious particle. Other important observations were incorporated into this model of the phage life cycle. For instance, August Doermann had reported that the phag ...
... could it live in harmony with its bacterial host, he noted. And, by some process of induction (stimulus), the prophage again became an infectious particle. Other important observations were incorporated into this model of the phage life cycle. For instance, August Doermann had reported that the phag ...
Molecular basis of the inflammatory response to adenovirus
... Adenovirus vectors are well known to induce host adaptive immunity.10 The Th1 dominant antiviral immune response occurs 5–7 days following transduction and is directed against the residual expression of viral genes that are still present in first-generation recombinant Ad-vectors.10,11 The developme ...
... Adenovirus vectors are well known to induce host adaptive immunity.10 The Th1 dominant antiviral immune response occurs 5–7 days following transduction and is directed against the residual expression of viral genes that are still present in first-generation recombinant Ad-vectors.10,11 The developme ...
The porcine reproductive and respiratory syndrome quandary. Part II
... stimulate immunity) are genetically engineered into the genome of another virus (the vector). The vector does not have to be a virus, for example it could be a bacterium, but I will focus on a virus for this short discussion. When a person or a lower animal ...
... stimulate immunity) are genetically engineered into the genome of another virus (the vector). The vector does not have to be a virus, for example it could be a bacterium, but I will focus on a virus for this short discussion. When a person or a lower animal ...
Document
... • Relatively easy to generate and renew • Infect a wide variety of cell types • Potential for in vivo gene delivery ...
... • Relatively easy to generate and renew • Infect a wide variety of cell types • Potential for in vivo gene delivery ...
What is a genome?
... The assembly starts when we generate the output of the sequencing machines This output can differ, but most of the time it will be a big FASTQ file maybe you know what a FASTA file is? ...
... The assembly starts when we generate the output of the sequencing machines This output can differ, but most of the time it will be a big FASTQ file maybe you know what a FASTA file is? ...
PDF
... system. To this end, we introduced I-SceI and I-CreI sources in TS51D flies simultaneously. As expected, flies of the R-G+W- type appeared in the progeny, but only in small numbers (4/2302; (Table 1). This small population can be explained by the light heat shock conditions that are suitable for I-C ...
... system. To this end, we introduced I-SceI and I-CreI sources in TS51D flies simultaneously. As expected, flies of the R-G+W- type appeared in the progeny, but only in small numbers (4/2302; (Table 1). This small population can be explained by the light heat shock conditions that are suitable for I-C ...
Molecular Determinants of Alphavirus Neurovirulence: Nucleotide
... D N A complementary to the 26S region of viral 42S RNA was prepared for cloning into pUC18 by priming at the T-end of the genome, as previously described (Kinney et al., 1986). Restriction fragments of the cloned cDNA, designated pTC-5, were subcloned into M 13 phages mpl0 and mpl 1. The entire sequ ...
... D N A complementary to the 26S region of viral 42S RNA was prepared for cloning into pUC18 by priming at the T-end of the genome, as previously described (Kinney et al., 1986). Restriction fragments of the cloned cDNA, designated pTC-5, were subcloned into M 13 phages mpl0 and mpl 1. The entire sequ ...
Quasispecies evolution of a hypervariable region of the feline
... sequences were obtained from isolates from these cats implying they were relatively homogeneous. This was confirmed by sequencing multiple clones of the isolates made from Sam 1 and 25 days p.i. in which nine of ten and seven of eight clones respectively, were identical to the consensus sequence for ...
... sequences were obtained from isolates from these cats implying they were relatively homogeneous. This was confirmed by sequencing multiple clones of the isolates made from Sam 1 and 25 days p.i. in which nine of ten and seven of eight clones respectively, were identical to the consensus sequence for ...
Adeno-associated virus
Adeno-associated virus (AAV) is a small virus which infects humans and some other primate species. AAV is not currently known to cause disease. The virus causes a very mild immune response, lending further support to its apparent lack of pathogenicity. Gene therapy vectors using AAV can infect both dividing and quiescent cells and persist in an extrachromosomal state without integrating into the genome of the host cell, although in the native virus some integration of virally carried genes into the host genome does occur. These features make AAV a very attractive candidate for creating viral vectors for gene therapy, and for the creation of isogenic human disease models. Recent human clinical trials using AAV for gene therapy in the retina have shown promise.AAV belongs to the genus Dependoparvovirus, which in turn belongs to the family Parvoviridae. The virus is a small (20 nm) replication-defective, nonenveloped virus.