Rapid generation of nested chromosomal
... alternative. As a result, a number of laboratories have created large deletions in mice (12–21). Currently there are a number of methods available for making deletions in ES cells. A review of them reveals several shortcomings. One type of strategy using Cre recombinase depends ...
... alternative. As a result, a number of laboratories have created large deletions in mice (12–21). Currently there are a number of methods available for making deletions in ES cells. A review of them reveals several shortcomings. One type of strategy using Cre recombinase depends ...
Raven/Johnson Biology 8e
... The correct answer is c— B. Answer b is incorrect. The DNA microarray is only sensitive to the presence or absence of an mRNA, not DNA. The correct answer is c—The pattern of gene expression C. Answer c is correct. DNA microarray technology uses fluorescent probes to examine the pattern of gene expr ...
... The correct answer is c— B. Answer b is incorrect. The DNA microarray is only sensitive to the presence or absence of an mRNA, not DNA. The correct answer is c—The pattern of gene expression C. Answer c is correct. DNA microarray technology uses fluorescent probes to examine the pattern of gene expr ...
Chapter 12 Molecular Genetics
... copies of the original double-stranded DNA to be produced. Notice in Figure 12.11 that the two strands are made in a slightly different manner. One strand is called the leading strand and is elongated as the DNA unwinds. This strand is built continuously by the addition of nucleotides to the 3' end. ...
... copies of the original double-stranded DNA to be produced. Notice in Figure 12.11 that the two strands are made in a slightly different manner. One strand is called the leading strand and is elongated as the DNA unwinds. This strand is built continuously by the addition of nucleotides to the 3' end. ...
CB3 - Homework
... beakers. Add 10 cm of washing-up liquid and stir in gently until it dissolves. Do not make the mixture foamy! B Mash 50 g of peas using the pestle and mortar. When they are as mashed as you can make them, put them into the other large empty beaker and add the solution you made in step A. Stir slowly ...
... beakers. Add 10 cm of washing-up liquid and stir in gently until it dissolves. Do not make the mixture foamy! B Mash 50 g of peas using the pestle and mortar. When they are as mashed as you can make them, put them into the other large empty beaker and add the solution you made in step A. Stir slowly ...
Snímek 1
... (5) meiotic elimination in some species is counter-balanced by processes of drive at mitosis, mainly in the gametophytes, and less frequently at meiosis (equilibrium frequencies in populations) (6) neutral effects; negative and quantitative effects on the phenotype when present in high numbers (redu ...
... (5) meiotic elimination in some species is counter-balanced by processes of drive at mitosis, mainly in the gametophytes, and less frequently at meiosis (equilibrium frequencies in populations) (6) neutral effects; negative and quantitative effects on the phenotype when present in high numbers (redu ...
The dual nature of homologous recombination in plants
... processes of life. It has a dual function through its activities both in meiosis and in somatic cells. It creates new linkages in the genetic material in meiosis and in many organisms it is even required for fertility. Indeed, HR substantially contributes to evolution. HR is also important for repai ...
... processes of life. It has a dual function through its activities both in meiosis and in somatic cells. It creates new linkages in the genetic material in meiosis and in many organisms it is even required for fertility. Indeed, HR substantially contributes to evolution. HR is also important for repai ...
array CGH - Unique The Rare Chromosome Disorder Support Group
... or duplicated in your child is interesting and may help guide future studies, it does not lead directly to immediate improved treatment. At present, we do not know the function and impact of many of our genes so it is not possible to predict what the consequence is of having a particular gene missin ...
... or duplicated in your child is interesting and may help guide future studies, it does not lead directly to immediate improved treatment. At present, we do not know the function and impact of many of our genes so it is not possible to predict what the consequence is of having a particular gene missin ...
Student
... words, DNA is the substrate. Since DNA prefers to be in a double helix, it will try to join back together. But during replication, a part of the double helix needs to remain open for the two strands to be read and copied. After helicase has opened a small section, a group of small proteins coat the ...
... words, DNA is the substrate. Since DNA prefers to be in a double helix, it will try to join back together. But during replication, a part of the double helix needs to remain open for the two strands to be read and copied. After helicase has opened a small section, a group of small proteins coat the ...
Genetic Portrait of a Yeast
... Large numbers of cells can easily be grown and assayed Mutations can be isolated in diploids, then analyzed in haploids Conditional mutants can be isolated Recombination frequencies are high, facilitating molecular manipulations Genome size is small and number of genes low, so analysis and manipulat ...
... Large numbers of cells can easily be grown and assayed Mutations can be isolated in diploids, then analyzed in haploids Conditional mutants can be isolated Recombination frequencies are high, facilitating molecular manipulations Genome size is small and number of genes low, so analysis and manipulat ...
Directions for Use Uracil-DNA Glycosylase (UNG), Cod
... Will UNG remove uracil from both ss- and dsDNA with the same efficiency? No, UNG removes uracil from ss-DNA at a rate of 200% compared to dsDNA. Will UNG remove uracil from RNA? Will it work with short oligos (e.g. 25-mer)? UNG does not act on uracil on the ribose sugar backbone. It will work on ...
... Will UNG remove uracil from both ss- and dsDNA with the same efficiency? No, UNG removes uracil from ss-DNA at a rate of 200% compared to dsDNA. Will UNG remove uracil from RNA? Will it work with short oligos (e.g. 25-mer)? UNG does not act on uracil on the ribose sugar backbone. It will work on ...
genotyping arabidopsis - STLCC.edu :: Users` Server
... concentration of the deoxynucleotides in the reaction is also critical. For example, if the concentration of dNTPs is too high, fidelity of the polymerase (ability to stay attached to the template DNA) will decrease. When the cycle is complete, two double-stranded DNA molecules will result, each con ...
... concentration of the deoxynucleotides in the reaction is also critical. For example, if the concentration of dNTPs is too high, fidelity of the polymerase (ability to stay attached to the template DNA) will decrease. When the cycle is complete, two double-stranded DNA molecules will result, each con ...
DNA - Lyndhurst Schools
... 6. Colorblindness is a sex-linked trait that affects males more often than it does females. It requires only one affected sex chromosome to be expressed in males, but in females it requires two affected sex chromosomes. On what chromosome do you think the trait for colorblindness is found? Explain. ...
... 6. Colorblindness is a sex-linked trait that affects males more often than it does females. It requires only one affected sex chromosome to be expressed in males, but in females it requires two affected sex chromosomes. On what chromosome do you think the trait for colorblindness is found? Explain. ...
High-Resolution Single-Copy Gene Fluorescence in Situ
... High-resolution cytogenetic maps provide important biological information on genome organization and function, as they correlate genetic distance with cytological structures, and are an invaluable complement to physical sequence data. The most direct way to generate a cytogenetic map is to localize ...
... High-resolution cytogenetic maps provide important biological information on genome organization and function, as they correlate genetic distance with cytological structures, and are an invaluable complement to physical sequence data. The most direct way to generate a cytogenetic map is to localize ...
Repeat mediated gene duplication in the Drosophila
... arm; the results presented are not affected by whether Muller elements or chromosome arms are used. Duplicated genes were assigned to one of three classes based on the relative position of the paralogs: adjacent, non-adjacent intra-chromosome-arm, or interchromosome-arm. Adjacent duplications have n ...
... arm; the results presented are not affected by whether Muller elements or chromosome arms are used. Duplicated genes were assigned to one of three classes based on the relative position of the paralogs: adjacent, non-adjacent intra-chromosome-arm, or interchromosome-arm. Adjacent duplications have n ...
DNA-dependent protein kinase in nonhomologous end joining: a
... against degradation or premature and incorrect ligation, it is clear that the DNA-PKCS cap has to be removed or altered before religation of the DNA ends and repair of the DSB can take place. Several authors have demonstrated that autophosphorylation of DNA-PKCS results in release of the cap and acc ...
... against degradation or premature and incorrect ligation, it is clear that the DNA-PKCS cap has to be removed or altered before religation of the DNA ends and repair of the DSB can take place. Several authors have demonstrated that autophosphorylation of DNA-PKCS results in release of the cap and acc ...
Woods Hole – Zebrafish Genetics and Development Bioinformatics
... in the genome, locate the translational start site (ATG), and identify the exonintron boundaries. Design two 25-mer morpholino sequences that target (1) the ATG and (2) an exon-intron boundary. Identify an orthologous gene in another fish species for use in rescue experiments to control for morpholi ...
... in the genome, locate the translational start site (ATG), and identify the exonintron boundaries. Design two 25-mer morpholino sequences that target (1) the ATG and (2) an exon-intron boundary. Identify an orthologous gene in another fish species for use in rescue experiments to control for morpholi ...
pdf
... A total of 92 DNA clones containing sequences identified as homologous with known nifH sequences were retrieved from seven of the nine depths from which clone libraries were made (Table 1). The cDNA clone libraries were made from five depths, and a total of 99 cDNA sequences were obtained (Table 1). ...
... A total of 92 DNA clones containing sequences identified as homologous with known nifH sequences were retrieved from seven of the nine depths from which clone libraries were made (Table 1). The cDNA clone libraries were made from five depths, and a total of 99 cDNA sequences were obtained (Table 1). ...
"PCR-based Species Identification and Gene Tagging"
... Detection of infected progeny from a systemically infected maize plant via dot-blot hybridization using genomic P. sorghi gDNA ...
... Detection of infected progeny from a systemically infected maize plant via dot-blot hybridization using genomic P. sorghi gDNA ...
Bioinformatics Molecular Genetics
... More precisely, the genome of an organism is a complete DNA sequence of one set of chromosomes; for example, one of the two sets that a diploid individual carries in every somatic cell. The term genome can be applied specifically to mean the complete set of nuclear DNA (i.e., the nuclear genome) but ...
... More precisely, the genome of an organism is a complete DNA sequence of one set of chromosomes; for example, one of the two sets that a diploid individual carries in every somatic cell. The term genome can be applied specifically to mean the complete set of nuclear DNA (i.e., the nuclear genome) but ...
Genomic library
A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.