Lecture 7 Oct 10th
... Genetic material sequenced after amplification using PCR (polymerase chain reaction) or used in fingerprinting approaches One study found that 6,000-10,000 unique genomes found in soil, compared to 40 cultured organisms ...
... Genetic material sequenced after amplification using PCR (polymerase chain reaction) or used in fingerprinting approaches One study found that 6,000-10,000 unique genomes found in soil, compared to 40 cultured organisms ...
Topic 4.4 - Genetic Engineering and Biotechnology
... enzyme recognises unique sequences of DNA in the plasmid and in the target DNA. It will cut DNA, producing “sticky ends”. Complementary sticky ends in target DNA and the plasmid allow incorporation of the target DNA into the plasmid, producing recombinant DNA. DNA ligase creates covalent bonds joini ...
... enzyme recognises unique sequences of DNA in the plasmid and in the target DNA. It will cut DNA, producing “sticky ends”. Complementary sticky ends in target DNA and the plasmid allow incorporation of the target DNA into the plasmid, producing recombinant DNA. DNA ligase creates covalent bonds joini ...
Study Guide – Unit 6 Test: Genetics and DNA Name: Per: 1 2 3 4 5 6
... What do genes contain instructions for? ...
... What do genes contain instructions for? ...
Ch 16-17 Practice Quiz
... • Thomas Hunt Morgan (fruit flies, linked genes) • Avery and colleagues : first proposed DNA as the transforming agent/hereditary agent • Mendel (early heredity) • Hershey-Chase show that DNA is the hereditary material • Griffith: transformation and mice ...
... • Thomas Hunt Morgan (fruit flies, linked genes) • Avery and colleagues : first proposed DNA as the transforming agent/hereditary agent • Mendel (early heredity) • Hershey-Chase show that DNA is the hereditary material • Griffith: transformation and mice ...
Mycoplasma genitalium
... • OK you have a sequence. What does the gene do? What is the function of the protein? – Search database for similar sequences – How does sequence compare to sequences for proteins of known function? – Use computer to search for functional motifs. • Various proteins that do the same thing have simila ...
... • OK you have a sequence. What does the gene do? What is the function of the protein? – Search database for similar sequences – How does sequence compare to sequences for proteins of known function? – Use computer to search for functional motifs. • Various proteins that do the same thing have simila ...
MODERN GENETICS USES DNA TECHNOLOGY
... (unique unless identical twin) to solve crimes. The more matches between crime scene DNA and suspect’s DNA, the higher the probability suspect is guilty. • Experts recommend 4-6 DNA regions be matched to establish guilt (chances are very small someone else would have the same DNA for this number of ...
... (unique unless identical twin) to solve crimes. The more matches between crime scene DNA and suspect’s DNA, the higher the probability suspect is guilty. • Experts recommend 4-6 DNA regions be matched to establish guilt (chances are very small someone else would have the same DNA for this number of ...
PowerPoint-Präsentation
... strain with a linear fragment containing a URA3 selectable marker flanked by homologous sequences. The chromosomal segment is replaced by this URA3 containing fragment after integration by homologous recombination. (B) The URA3 marker introduced in the YFG1 locus, can be excised if URA3 is also flan ...
... strain with a linear fragment containing a URA3 selectable marker flanked by homologous sequences. The chromosomal segment is replaced by this URA3 containing fragment after integration by homologous recombination. (B) The URA3 marker introduced in the YFG1 locus, can be excised if URA3 is also flan ...
Genetic Engineering
... • Recombinant DNA is taking DNA from one source and inserting it into an organism’s DNA, giving that organism those characteristics. – Ex.= Inserting salmon’s anti-freezing genes into corn to allow it to survive frost. ...
... • Recombinant DNA is taking DNA from one source and inserting it into an organism’s DNA, giving that organism those characteristics. – Ex.= Inserting salmon’s anti-freezing genes into corn to allow it to survive frost. ...
Name: Date: Period: ______ Notes Questions for the Unit 12, Part 2
... 5. A restriction fragment analysis was carried out on DNA samples taken from members of a family due to questionable paternity of one of the family’s children. The results of the gel generated for analysis are shown to the right. Which of the following statements is supported by the data? Explain yo ...
... 5. A restriction fragment analysis was carried out on DNA samples taken from members of a family due to questionable paternity of one of the family’s children. The results of the gel generated for analysis are shown to the right. Which of the following statements is supported by the data? Explain yo ...
1.) Plasmids ______.
... onto an island far offshore and manages to survive and reproduce there for a period of 10,000 years. After that period, a climate change results in lower sea levels and the reconnection of the island with the mainland. Members of the formerly isolated island finch population can now interact freely ...
... onto an island far offshore and manages to survive and reproduce there for a period of 10,000 years. After that period, a climate change results in lower sea levels and the reconnection of the island with the mainland. Members of the formerly isolated island finch population can now interact freely ...
Public data and tool repositories Section 2 Survey of
... 1. Integrates feature identity information with whole genome view 2. Allows one to view and search an organism's complete genome 3. Displays chromosome maps 4. User can zoom into progressively greater levels of detail, down to the sequence data for a region of interest. 5. Focus more on individual s ...
... 1. Integrates feature identity information with whole genome view 2. Allows one to view and search an organism's complete genome 3. Displays chromosome maps 4. User can zoom into progressively greater levels of detail, down to the sequence data for a region of interest. 5. Focus more on individual s ...
CHAPTER 12
... 1. Explain how recombinant DNA technology can be used to produce useful products. 2. Explain how recombinant DNA techniques are used to mass-produce a protein from an isolated gene; such as Humulin, human growth hormone, erythropoietin.. 3. Explain how restriction enzymes and DNA ligase are used to ...
... 1. Explain how recombinant DNA technology can be used to produce useful products. 2. Explain how recombinant DNA techniques are used to mass-produce a protein from an isolated gene; such as Humulin, human growth hormone, erythropoietin.. 3. Explain how restriction enzymes and DNA ligase are used to ...
Document
... iii. Has anyone ever been born without an X chromosome? ______ 11. What is the purpose of a karyotype? a. List 3 things a karyotype shows? b. What does homologous chromosomes mean? 12. Human gametes contain ______ autosomes and ______ sex chromosomes each. 13. In pedigrees, males are drawn as a ____ ...
... iii. Has anyone ever been born without an X chromosome? ______ 11. What is the purpose of a karyotype? a. List 3 things a karyotype shows? b. What does homologous chromosomes mean? 12. Human gametes contain ______ autosomes and ______ sex chromosomes each. 13. In pedigrees, males are drawn as a ____ ...
Study_Guide
... State that deoxyribonucleic acid (DNA) is a polynucleotide, usually double-stranded, made up of nucleotides containing the bases adenine (A), thymine (T), cytosine (C) and guanine (G). State that ribonucleic acid (RNA) is a polynucleotide, usually single-stranded, made up of nucleotides containi ...
... State that deoxyribonucleic acid (DNA) is a polynucleotide, usually double-stranded, made up of nucleotides containing the bases adenine (A), thymine (T), cytosine (C) and guanine (G). State that ribonucleic acid (RNA) is a polynucleotide, usually single-stranded, made up of nucleotides containi ...
B. gal-4 and gal-7
... the precursor ribosomal RNA genes are transcribed and then processed into mature rRNAs viz. 5.8s. Identification of rRNA processing 17S and 26S. This processing of pre-rRNA is believed to be regulated by protein products of gene homologs of yeast in specific genes. In yeast (Saccharomyces cerevisiae ...
... the precursor ribosomal RNA genes are transcribed and then processed into mature rRNAs viz. 5.8s. Identification of rRNA processing 17S and 26S. This processing of pre-rRNA is believed to be regulated by protein products of gene homologs of yeast in specific genes. In yeast (Saccharomyces cerevisiae ...
Advances in Genetics
... Cows then produce clotting protein in milk, which can then be extracted for humans. Gene Therapy • Working copies of a gene inserted directly into cells of a person with a genetic disorder • Used to correct some genetic disorders ...
... Cows then produce clotting protein in milk, which can then be extracted for humans. Gene Therapy • Working copies of a gene inserted directly into cells of a person with a genetic disorder • Used to correct some genetic disorders ...
Genomic library
A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.