• Study Resource
  • Explore Categories
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
SEG exam 2 1
SEG exam 2 1

... part of the short arm (p arm) of the Y chromosome. These females have poorly developed (streak) gonads and are sterile. Individuals who are 46,XY and missing part of the long arm (q arm) of the Y are also known but these individuals appear to be normal males. What does this suggest about the locatio ...
Chapter 21: Genomics I: Analysis of DNA and Transposable Elements
Chapter 21: Genomics I: Analysis of DNA and Transposable Elements

... because they are inherently mobile. They were discovered by Barbara McClintock in the 1950s through her classic studies with corn. Since then, geneticists have discovered many different types of TEs in organisms as diverse as bacteria, fungi, plants, and animals. The text first explores the three ma ...
A general video on DNA sequencing is
A general video on DNA sequencing is

... b. You want to amplify it by PCR, so you must make two primers for PCR. Why are there two, and what sequences are they? c. The gene coding for myoglobin, is on chromosome 22, which is 49 million DNA base pairs. (Chromosome 22 is actually a small chromosome, representing between 1.5 and 2% of the tot ...
Section D - Prokaryotic and Eukaryotic Chromosome Structure
Section D - Prokaryotic and Eukaryotic Chromosome Structure

... • Replication of phage λin vivo produces long linear molecules with multiple copies of the λ genome. These concatemers are ...
Mutations
Mutations

... way a complex organism develops from single fertilized cell. 1. Hox genes- controls organs and tissues that develop in various parts of the embryo a. Mutation in one of these “master control genes” can completely change organs that develop in specific parts of the body b. Genes tell cells in the bod ...
Genomic research concepts and application
Genomic research concepts and application

... Solid phase support to hold large  numbers of individual DNA molecules Sequencing via multiple cycles of  incorporation using different  fluorophores for each nucleotide Images per cycle provide sequence  data Based on short reads from a large  number of molecules Jay Shendure & Hanlee Ji Nature Bio ...
Invertebrate epigenomics: the brave new world of
Invertebrate epigenomics: the brave new world of

... mechanisms in invertebrate development, caste specification, homeostasis and evolution. To begin with, Maleszka and colleagues put forward an exciting theory of how genomic and ...
Invention Fact Sheet  - Lemelson
Invention Fact Sheet - Lemelson

... fold up into a double helix, but have not fully understood how the three billion base-pair long genome – 2 meters in length when stretched out – folds to fit into the nucleus of a human cell, which is less than one hundredth of a millimeter in diameter. To answer this question, Erez Lieberman-Aiden ...
CS691K Bioinformatics Kulp Lecture Notes #0 Molecular
CS691K Bioinformatics Kulp Lecture Notes #0 Molecular

... Humans: 23 pairs of chromosomes. Total ~3B “bases” (x2) DNA resides in nucleus in eukaryotes ...
ChIP-seq
ChIP-seq

... •Identifying genes and annotating regulatory function within and among genomes •Computational issues: data normalization, peak calling, differential expression and binding •Large-scale studies revealing regulatory architecture of human & model genomes ...
Microbial Genetics
Microbial Genetics

Recombinant DNA technology
Recombinant DNA technology

... • This new recombinant virus is used as a vaccine. • These vaccines are much safer since they do not expose the patients to the actual virus and do not risk to infection. • This method may be useful in vaccines against malaria and schistosomiasis and many viruses (e.g. HBV) ...
J. Bacteriol.-2012-H
J. Bacteriol.-2012-H

... molecular basis of M. vaccae and further study phylogenetic relationships and the genetic factors responsible for pathogenicity, we determined the complete genome sequence of this microorganism. Whole-genome sequencing is also important to facilitate a more reliable genetic identification between an ...
PLASMIDS AND RESTRICTION ENZYMES
PLASMIDS AND RESTRICTION ENZYMES

... conjugation, which enables bacteria to share and exchange genetic information. When a plasmid with a gene for antibiotic resistance is taken in by bacteria lacking that plasmid, the bacteria will then become resistant to that specific antibiotic. In nature, conjugation occurs with a very low efficie ...
Human Genetics
Human Genetics

... More diseases are now being understood in terms of gene expression. As our knowledge of and technology in this field increase, bioethical issues arise that preceding generations could not have imagined. ...
THINK ABOUT THESE………………
THINK ABOUT THESE………………

... 52. Because bryophytes lack _____________ tissue, they must grow close to the ground 53. What are the two types of vascular tissue? 54. What moves the products of photosynthesis from source to sink? 55. Where does the embryo develop in flowering plants? 56. Where are pollen grains made and stored? 5 ...
Syllabus (Principles of Biotechnology) File
Syllabus (Principles of Biotechnology) File

... hybridization; Gene libraries; PCR amplification; Plant and animal cell and tissue culture techniques and their applications. UNIT III Molecular markers and their applications; DNA sequencing; Applications of gene cloning in basic and applied research; Genetic engineering and transgenics; Genomics, ...
Human Genomic DNA Quality Controls for aCGH and Microarray
Human Genomic DNA Quality Controls for aCGH and Microarray

Chapt24 slides - Workforce3One
Chapt24 slides - Workforce3One

... - Polyploidization can lead to jumping of transposon • Transposon can move to new position in genome giving rise to new phenotypes ...
Biotechnology
Biotechnology

... Compare and contrast biotechnology, recombinant DNA technology, and genetic engineering. Identify the roles of a clone and a vector in making recombined DNA. Compare selection and mutation. Define restriction enzymes, and outline their use to make recombinant DNA. List some properties of vectors and ...
DNA Technology
DNA Technology

... • Avoids eukaryotic-prokaryotic incompatibility • Yeast – single-celled, easy to grow, have plasmids • Scientists have constructed vectors called yeast artificial chromosomes (YACs) -have an origin for DNA replication, a centromere, and two telomeres -behave normally in mitosis – foreign DNA cloned ...
Prokaryotes, Viruses, and Protistans
Prokaryotes, Viruses, and Protistans

... • Complete part of the life cycle inside specific cells of a host organism • Many have elaborate life cycles that require different hosts • Many cause serious human disease ...
DNA notes
DNA notes

... replication forks (closed triangles). Chromosome markers are segregated progressively as they are replicated, finishing with the terminus. ...
Unit 2 Review
Unit 2 Review

... Use the following as a TOOL only. Survey to see what you don’t know, focus on these terms in your notes. This will not be covered in class other than process questions you and several others are concerned about. Questions that are straight from the notes will be re-directed to the notes—find them th ...
Basic Principles of Protein Chemistry
Basic Principles of Protein Chemistry

... to prepare template DNA ...
< 1 ... 490 491 492 493 494 495 496 497 498 ... 561 >

Genomic library



A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.
  • studyres.com © 2025
  • DMCA
  • Privacy
  • Terms
  • Report