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Ch. 9: Presentation Slides
Ch. 9: Presentation Slides

...  Plasmids are most convenient for cloning relatively small DNA fragments (5-10 kb)  Fragments (from 12-20 kb) can be cloned with bacteriophage   Still larger DNA fragments (40-45 kb) can be inserted into cosmid vectors. These vectors can exist as plasmids, but they also can be packaged into matu ...
DNA, RNA, PROTEINS STARTS WITH
DNA, RNA, PROTEINS STARTS WITH

... _C_ __ __ __ __ __ __ __ __ . 2. The group of 3 nitrogen bases in the mRNA message that is read together is called a _C_ __ __ __ __. 3. In dividing cells, the DNA is scrunched into _C_ __ __ __ __ __ __ __ __ __ __ so it can be moved. 4. The mRNA message tells the ribosomes which _A_ __ __ __ __ _A ...
PCR and Its Applications
PCR and Its Applications

Genoombrowsers - Radboud Universiteit
Genoombrowsers - Radboud Universiteit

... • With the UCSC browser one can examine genomic conservation ...
Abstract-Template-2016
Abstract-Template-2016

... During analysis of the promoter region of the human MEST gene, we noted apparent nonMendelian behaviour of three closely linked single nucleotide polymorphisms (SNPs) 1. MEST plays a role in mammalian development and maternal behaviour. It is genomically imprinted, with the maternally inherited alle ...
epigenome
epigenome

Abstract-Template-2017 - Queenstown Research Week
Abstract-Template-2017 - Queenstown Research Week

... During analysis of the promoter region of the human MEST gene, we noted apparent nonMendelian behaviour of three closely linked single nucleotide polymorphisms (SNPs) 1. MEST plays a role in mammalian development and maternal behaviour. It is genomically imprinted, with the maternally inherited alle ...
Human Genetics WF, ML , SFdf
Human Genetics WF, ML , SFdf

... One strand of DNA contains many genes.  DNA is found in our blood, as blood runs throughout our whole body and can be seen as an easy way to distribute DNA  If you pulled the DNA from a single human cell, the strand would be more than one meter long! ...
XL-I
XL-I

... Hb demonstrates higher oxygen carrying capacity compared to myoglobin II. There is covalent bonding between the four subunits of Hb III. During deoxygenation the loss of the first oxygen molecule from oxygenated Hb promotes the dissociation of oxygen from the other subunits (A) II ...
Practice Exam II
Practice Exam II

... fastens DNA fragments together E selectable marker in plasmid vector C may be used to target specific tissues ...
Epigenetic modification of DNA
Epigenetic modification of DNA

... genome-wide DNA methylation patterns of all human genes in all major tissues. • Methylation is tissue specific and is of major importance in the regulation of gene expression during development. ...
DNA,RNA & Protein synthesis game
DNA,RNA & Protein synthesis game

... Hershey and Chase found this radioactively tagged element inside the cell after subjecting bacteria to viruses. ...
DNA Technology
DNA Technology

... The HGP • Since the completion of the HGP, two monumental applications (uses) have been discovered): 1. Disease Prediction: scientists can look at specific genes and tell if you inherited a disease. 2. Gene Therapy: if you have a defective gene, it can now be corrected ...
FAQ of Module 7
FAQ of Module 7

... (a) Gene and Genome: The segment of DNA that carries genetic information are called genes. Genes hold the information to build and maintain an organism's cells and pass genetic traits to offspring. Genome is the sum total of an organism's hereditary information. It is encoded either in DNA or, for m ...
DNA Technology
DNA Technology

... How do you make DNA fingerprints? • RFLP (restriction fragment length polymorphism) analysis ▫ 1. extract DNA from specimen using restriction enzymes ▫ 2. separate fragments of DNA using electrophoresis (separates DNA according to size and charge) ▫ 3. placed in wells made on gel and run electric c ...
The brain and spinal cord comprise the central nervous system
The brain and spinal cord comprise the central nervous system

... • Barr Body – concept of X-Chromosome Inactivation and Dosage Compensation • Nondisjunction of autosomes (example Down Syndrome) and Sex Chromosomes • In utero detection methods for genetic defects: Amniocentesis, Chorionic villi sampling • Exceptions to the Chromosomal Theory of Inheritance: Concep ...
Gene: Usually, a section of DNA long enough to code for a protein
Gene: Usually, a section of DNA long enough to code for a protein

... one allele may code for brown eyes, the other for blue. Because blue is recessive, your phenotype would be brown. Phenotype: Describes the effect of a gene. White fur is a phenotype. Genotype: Describes the genes an organism has inherited. In class a genotype might be written as BB. Homozygous: The ...
Coevolution --- viruses may have evolved along with cells
Coevolution --- viruses may have evolved along with cells

... and nonsense (-, or noncoding) when single stranded --- most viral genomes make use of overlapping reading frames to get more use out of their small genomes ...
DNA WebQuest - Pearland ISD
DNA WebQuest - Pearland ISD

... Take the tour of DNA by clicking on “What is DNA?” and answer the questions below: 1. In what organelle (CELL PART) would I find your DNA (YOUR INSTRUCTIONS)? 2. What does DNA stand for? 3. The DNA molecule comes in the form of a ...
Biotechnology
Biotechnology

...  Let’s take a closer look at this… ...
9.4 Genetic Engineering
9.4 Genetic Engineering

Document
Document

... New genes can be added to an organism’s DNA. • Genetic engineering involves changing an organism’s DNA to give it new traits. • Genetic engineering is based on the use of recombinant DNA. • Recombinant DNA contains genes from more than one organism. ...
9.4 Genetic Engineering
9.4 Genetic Engineering

... New genes can be added to an organism’s DNA. • Genetic engineering involves changing an organism’s DNA to give it new traits. • Genetic engineering is based on the use of recombinant DNA. • Recombinant DNA contains genes from more than one organism. ...
9.4 Genetic Engineering KEY CONCEPT DNA sequences of organisms can be changed.
9.4 Genetic Engineering KEY CONCEPT DNA sequences of organisms can be changed.

... New genes can be added to an organism’s DNA. • Genetic engineering involves changing an organism’s DNA to give it new traits. • Genetic engineering is based on the use of recombinant DNA. • Recombinant DNA contains genes from more than one organism. ...
Name _________KEY___________________________
Name _________KEY___________________________

... human DNA and bacterial plasmid DNA. 2) Human gene is inserted into bacteria and the two combine. 3) The gene is cloned as bacteria reproduce. 4) Bacteria with the desired human gene are isolated to produce the protein of interest (insulin, for example). 38. Define the following terms: a. Recombinan ...
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Genomic library



A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.
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