DNA REPLICATION HANDOUT
... 1) Template strands: Original DNA strands that were ripped apart. 2) Replication Fork: Y-shaped region where new strands of DNA are elongated 3) Okazaki Fragments: Only found on the lagging strand. Since DNA is connected by base pairs, as the original strand “unzips” one of the templates is running ...
... 1) Template strands: Original DNA strands that were ripped apart. 2) Replication Fork: Y-shaped region where new strands of DNA are elongated 3) Okazaki Fragments: Only found on the lagging strand. Since DNA is connected by base pairs, as the original strand “unzips” one of the templates is running ...
4 chapter_test_b 4 chapter_test_b
... Name ______________________________ Class___________________Date__________________ ...
... Name ______________________________ Class___________________Date__________________ ...
forensics - bayo2pisay
... Also known as genetic fingerprinting or DNA profiling Way of identifying an individual* ...
... Also known as genetic fingerprinting or DNA profiling Way of identifying an individual* ...
File
... Heredity is the passing on of features from parents to offspring by means of genes Also called Genetic Inheritance ...
... Heredity is the passing on of features from parents to offspring by means of genes Also called Genetic Inheritance ...
Lecture Notes with Key Images
... Using pea plants, Mendel revealed the fundamental principles of transmission genetics. Work by others showed that genes are on chromosomes and that mutant strains can be used to map genes on chromosomes. ...
... Using pea plants, Mendel revealed the fundamental principles of transmission genetics. Work by others showed that genes are on chromosomes and that mutant strains can be used to map genes on chromosomes. ...
Solutions to 7.014 Problem Set 7
... 1) Digest mouse genomic DNA with a restriction enzyme and digest a plasmid vector with the same restriction enzyme. 2) Ligate the mouse DNA into the vector. 3) Transform ampicillin and kanamycin sensitive bacteria with this ligation mixture. 4) Plate the transformed bacteria on media containing ampi ...
... 1) Digest mouse genomic DNA with a restriction enzyme and digest a plasmid vector with the same restriction enzyme. 2) Ligate the mouse DNA into the vector. 3) Transform ampicillin and kanamycin sensitive bacteria with this ligation mixture. 4) Plate the transformed bacteria on media containing ampi ...
No Slide Title
... Base-specific chemical reaction Different sets of chemical reactions Dimethyl sulfate selectively react to purine Hydrazine selectively react to pyrimidine ...
... Base-specific chemical reaction Different sets of chemical reactions Dimethyl sulfate selectively react to purine Hydrazine selectively react to pyrimidine ...
Genetica per Scienze Naturali aa 05
... Following genome duplication, an initially diploid cell could have undergone a transient tetraploid state; subsequent large-scale chromosome inversions and translocations, etc., could result in chromosome divergence and restore diploidy, but now with twice the number of chromosomes Following duplica ...
... Following genome duplication, an initially diploid cell could have undergone a transient tetraploid state; subsequent large-scale chromosome inversions and translocations, etc., could result in chromosome divergence and restore diploidy, but now with twice the number of chromosomes Following duplica ...
Timeline Code DNAi Site Guide
... FISH for information about your chromosomes: Centromeres, Telomeres, Variation Genome spots Click on a "spot" to find out about the gene or genes at that location ...
... FISH for information about your chromosomes: Centromeres, Telomeres, Variation Genome spots Click on a "spot" to find out about the gene or genes at that location ...
Gel Electrophoresis DNA Fingerprinting
... • In this hypothetical case, DNA was extracted from samples obtained from the five possible suspects, and the crime scene sample • You will cleave the DNA with a restriction enzyme and simulated a “mock” DNA fingerprint analysis using Southern Blotting ...
... • In this hypothetical case, DNA was extracted from samples obtained from the five possible suspects, and the crime scene sample • You will cleave the DNA with a restriction enzyme and simulated a “mock” DNA fingerprint analysis using Southern Blotting ...
Questions - Humble ISD
... Did you memorize or learn about DNA 1. What is the shape of DNA? Who determined this shape? 2. What biomolecule does DNA belong to? 3. What is the monomer of DNA. 4. What are the 3 parts of the monomer? 5. A single-ringed N-base is called _____ & includes ________ & _______ 6. A double-ringed N-base ...
... Did you memorize or learn about DNA 1. What is the shape of DNA? Who determined this shape? 2. What biomolecule does DNA belong to? 3. What is the monomer of DNA. 4. What are the 3 parts of the monomer? 5. A single-ringed N-base is called _____ & includes ________ & _______ 6. A double-ringed N-base ...
Immunology
... sequence in the variable region – the basis of this unique sequence lies in the organization of the immunoglobulin genes – these genes, however, have to be rearranged to become a functional immunoglobulin gene ...
... sequence in the variable region – the basis of this unique sequence lies in the organization of the immunoglobulin genes – these genes, however, have to be rearranged to become a functional immunoglobulin gene ...
Attenuated Infectious Hematopoietic Necrosis Virus (IHNV)
... The genome of the Infectious Hematopoietic Necrosis Virus (IHNV), a salmonid Novirhabdovirus has been engineered to modify the gene order and to evaluate the impact on a possible attenuation of the virus in vitro and in vivo. By reverse genetics, eight recombinant IHNV (rIHNV), termed NxGy according ...
... The genome of the Infectious Hematopoietic Necrosis Virus (IHNV), a salmonid Novirhabdovirus has been engineered to modify the gene order and to evaluate the impact on a possible attenuation of the virus in vitro and in vivo. By reverse genetics, eight recombinant IHNV (rIHNV), termed NxGy according ...
Chapter 20: Biotechnology - Biology E
... The complete set of plasmid-containing cell clones, each carrying copies of a particular segment from the initial genome, is referred to as a genomic library. Each “plasmid clone” in the library is like a book containing specific information. Historically, certain bacteriophages have also been used ...
... The complete set of plasmid-containing cell clones, each carrying copies of a particular segment from the initial genome, is referred to as a genomic library. Each “plasmid clone” in the library is like a book containing specific information. Historically, certain bacteriophages have also been used ...
C10 Lesson 3
... 1. _______________ In the process of cloning, breeders cross two genetically different individuals. 2. _______________ Crossing two individuals that have similar desirable characteristics is called genetic engineering. 3. _______________ In selective breeding, organisms with desired traits are chose ...
... 1. _______________ In the process of cloning, breeders cross two genetically different individuals. 2. _______________ Crossing two individuals that have similar desirable characteristics is called genetic engineering. 3. _______________ In selective breeding, organisms with desired traits are chose ...
Document
... Phage vectors are modified bacterial viruses -Most based on phage lambda (l) of E. coli -Used to clone inserts up to 40 Kbp -Have two features not shared with plasmid vectors -They kill their host cells -They have linear genomes -Middle replaced with inserted DNA ...
... Phage vectors are modified bacterial viruses -Most based on phage lambda (l) of E. coli -Used to clone inserts up to 40 Kbp -Have two features not shared with plasmid vectors -They kill their host cells -They have linear genomes -Middle replaced with inserted DNA ...
Whole Genome Scale DNA Methylation Differences in
... Summary of Results: We identified 132 different CpG sites at which the direction of the intra-MZ pair DNA methylation difference significantly correlated with the diabetic state i.e. T1D-associated methylation variable positions (T1D-MVPs). We confirmed these T1D-MVPs display statistically significa ...
... Summary of Results: We identified 132 different CpG sites at which the direction of the intra-MZ pair DNA methylation difference significantly correlated with the diabetic state i.e. T1D-associated methylation variable positions (T1D-MVPs). We confirmed these T1D-MVPs display statistically significa ...
Word Definition Synonym 1 heredity the passing of physical traits or
... a characteristic that an organism can pass on to its offspring through its genes allele the different forms of a gene like smooth/wrinkled seeds or tall/short height Rosalind Franklin famous woman scientist who used x-rays to photograph DNA molecules James Watson & Francis Crick with Franklin's phot ...
... a characteristic that an organism can pass on to its offspring through its genes allele the different forms of a gene like smooth/wrinkled seeds or tall/short height Rosalind Franklin famous woman scientist who used x-rays to photograph DNA molecules James Watson & Francis Crick with Franklin's phot ...
The Secret of How Life Works - The Biotechnology Institute
... times, though, the variations can make for happy surprises. It is important to understand that the word mutation doesn’t apply just to things that go wrong. Scientists also use the word to mean all the different variations that make us so diverse and interesting. Without variations, everyone would l ...
... times, though, the variations can make for happy surprises. It is important to understand that the word mutation doesn’t apply just to things that go wrong. Scientists also use the word to mean all the different variations that make us so diverse and interesting. Without variations, everyone would l ...
Chapters 19-21 review
... 16. The uptake of foreign “naked” DNA by bacteria is called _________________ ...
... 16. The uptake of foreign “naked” DNA by bacteria is called _________________ ...
Name___________ Midterm Review 1. What is an organism? 2
... 11. What molecule contains the cells hereditary information? 12. What is a gene? 13. New cells or organisms from asexual reproduction have information. 14. Name a unicellular organism that reproduces by asexual reproduction. 15. Define autotroph. ...
... 11. What molecule contains the cells hereditary information? 12. What is a gene? 13. New cells or organisms from asexual reproduction have information. 14. Name a unicellular organism that reproduces by asexual reproduction. 15. Define autotroph. ...
Methods S1: Vector constructions and transformation of yeast and
... (which introduces a NotI restriction site and a sequence encoding for a hexa-histidine tag). AvrLm4-7 PCR product was digested by SnaBI and NotI. AvrLm4-7 fragment was ligated into SnaBI-NotI digested pPIC9. The construct, termed pPIC9-A47, was cloned in E. coli, reextracted and sequenced. Preparati ...
... (which introduces a NotI restriction site and a sequence encoding for a hexa-histidine tag). AvrLm4-7 PCR product was digested by SnaBI and NotI. AvrLm4-7 fragment was ligated into SnaBI-NotI digested pPIC9. The construct, termed pPIC9-A47, was cloned in E. coli, reextracted and sequenced. Preparati ...
Genomic library
A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.