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Genetics Terminology List - Arabian Horse Association
Genetics Terminology List - Arabian Horse Association

... Genetic code - the instructions in a gene that tell the cell how to function. Genetic marker - a gene or other identifiable portion of DNA whose inheritance can be followed. Heterozygote - an individual having two different alleles of a particular gene; the individual can pass either allele to their ...
DNA and RNA - Xavier High School
DNA and RNA - Xavier High School

... What is meant by term base pairing? How is base pairing involved in DNA replication? When a DNA molecule is replicated, how do the new molecules relate to the original molecule? What is the difference between introns and exons? What is a codon?anticodon? How do they relate? Explain why controlling p ...
PLASMID ISOLATIONS (MINIPREPS)
PLASMID ISOLATIONS (MINIPREPS)

... combination of these. After the bacterial cells have been lysed, the plasmid is separated from the chromosomal DNA usually by differential precipitation of the DNAs. All rely on the different characteristics of the high molecular weight chromosomal DNA and the low molecular weight plasmid DNA. Highe ...
Heredity and Genetics Vocabulary
Heredity and Genetics Vocabulary

... Cross-Fertilization – A gamete(sex cell) from each parent gives one allele for each trait to make a new organism(humans) # of human chromosomes – 23 pairs or 46 chromosomes X and Y – Human chromosomes that determine gender Mutation – When a chromosome is not copied correctly Carrier – Someone who ha ...
Ribosome and Introduction to DNA Forensics
Ribosome and Introduction to DNA Forensics

Logic, DNA, and Poetry
Logic, DNA, and Poetry

... But the highway proved to be little more than a long, rutted detour. The straightforward, neatly determining logical structure envisioned by Crick — a structure the lust for which became a feverish obsession during the Human Genome Project — has progressively transformed itself into a seething cauld ...
Polyploidy
Polyploidy

... Cell volume generally rises with increasing genome size, although the exact relationship between ploidy and cell volume varies among environments and among taxa. Although average cell size is larger in polyploids, the size of the adult polyploidy organism may not be altered. As a rough generalizatio ...
Virus
Virus

... • In the lysogenic cycle, the phage genome replicates without destroying the host cell. • Temperate phages, like phage lambda, use both lytic and lysogenic cycles. • Within the host, the virus’ circular DNA engages in either the lytic or lysogenic cycle. • During a lytic cycle, the viral genes imme ...
MCB Lecture 1 – Molecular Diagnostics
MCB Lecture 1 – Molecular Diagnostics

... What is the typical size of fragments that PCR can amplify? o >1kb How many cycles must you perform via PCR before you get the first exact sample that you want to amplify? o 4 Cycles If you have a single base difference in sequence that does not affect a restriction site, how do you detect it? o Use ...
DNA packing - local.brookings.k12.sd.us
DNA packing - local.brookings.k12.sd.us

...  in humans, at least 5% of genome is made of a family of similar sequences called, Alu elements ...
DNA Packing
DNA Packing

... 2. Recombinant DNA technology/ Genetically Modified organisms – Recombinant DNA is formed by joining DNA sequences from two different sources: ...
Investigation 3: DNA - connorericksonbiology
Investigation 3: DNA - connorericksonbiology

... fact that living things inherit traits from their parents has been used since prehistoric times to improve crop plants and animals through selective breeding. Genes correspond to regions within DNA, a molecule composed of a chain of four different types of nucleotides—the sequence of these nucleotid ...
CHNOPS- Simulating Protein Synthesis
CHNOPS- Simulating Protein Synthesis

... blocks of proteins) that our cells make. The sequence of nucleotides in DNA determines the sequence of amino acids in polypeptides, and thus the structure of proteins. In a process called transcription, which takes place in the NUCLEUS of the cell, RNA Polymerase reads and copies the DNA’s nucleotid ...
Directed Reading A
Directed Reading A

... ______ 2. What is the name of the material that determines inherited characteristics? a. deoxyribonucleic acid c. RNA b. ribosome d. amino acid ...
Biologic
Biologic

... Recent observations on duplications provide possible mechanisms for change in gene function. When duplications between vertebrate genomes are compared as we get more and more complete data sets, it becomes clear that this mechanism of genetic change produces non-identical repeats in chromosomes (dup ...
Issues in Biotechnology
Issues in Biotechnology

... Gel electrophoresis is an important tool in molecular biology and biotechnology. Electro refers to the energy of electricity. Phoresis, from the Greek verb phoros, means “to carry across.” Thus, gel electrophoresis refers to the technique in which molecules are forced across a span of gel, motivated ...
DNA as Genetic Material
DNA as Genetic Material

... could be made pathogenic by incubating with heatkilled pathogenic bacteria - bacteria were “transformed” ...
Notes
Notes

... - F2 generation (2nd filial) is offspring made by F1 x F1 ...
BIOTECHNOLOGY
BIOTECHNOLOGY

... endonuclease isolated from that strain. EcoRI - from Escherichia coli BamHI - from Bacillus amyloliquefaciens HindIII - from Haemophilus influenzae (the one H. Smith found) PstI - from Providencia stuartii Sau3AI - from Staphylococcus aureus AvaI - from Anabaena variabilis ...
Biology Lecture 2 – Genes
Biology Lecture 2 – Genes

“Ancient” Viruses
“Ancient” Viruses

...  Adenoviruses are non-enveloped Icosahedral viruses with spike-like projections at the vertices.  Contain a linear double stranded DNA genome of about 36 KB that accounts for about 13% of mass of the virus particle.  First found in 1957 in the adenoid tissues of US Army recruits.  More than 40 A ...
Structural Variations
Structural Variations

... genetic differentiation and comparison of overall effect of population substructure. Measures reduction in heterozygosity (H) expected with non-random mating at any one level of population hierarchy relative to another more inclusive hierarchical level. ...
Ch. 10 Vocabs
Ch. 10 Vocabs

... -Replication fork: a Y-shaped point that results when the two strands of a DNA double helix separate so that the DNA molecule can be replicated. -DNA polymerase: an enzyme that catalyzes the formation of the DNA molecule. -Semi-conservative replication: in each new DNA double helix, one strand is fr ...
Datasheet - Santa Cruz Biotechnology
Datasheet - Santa Cruz Biotechnology

... MND1 localizes to chromatin during meiotic prophase and preferentially binds double-stranded DNA. MND1 forms a stable heterodimeric complex with HOP2, which binds DNA to activate the recombinase activity of DMC1 and RAD51. Disruption of the MND1-HOP2 complex leads to failure in meiotic recombination ...
Process of Electrophoresis
Process of Electrophoresis

... Agarose gel electrophoresis is a procedure used to separate DNA fragments based on their sizes. DNA is an acid and has many negative electrical charges. Scientists have used this fact to design a method that can be used to separate pieces of DNA. A solution containing a mixture of DNA fragments of v ...
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Genomic library



A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.
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