Precise insertion and guided editing of higher plant
... (crRNAs) and generate double strand breaks of DNA respectively12. The dual enzymatic activities allow for multiplex targeting from a single crRNA transcript13. In addition, Cpf1 nucleases have also been shown to have lower rates of off target edits relative to Cas9 nucleases14, 15. Cpf1 nucleases in ...
... (crRNAs) and generate double strand breaks of DNA respectively12. The dual enzymatic activities allow for multiplex targeting from a single crRNA transcript13. In addition, Cpf1 nucleases have also been shown to have lower rates of off target edits relative to Cas9 nucleases14, 15. Cpf1 nucleases in ...
Analysing complex genetic traits with chromosome
... for the strain combination of interest. For the mouse, a total of 21 strains must be constructed through a ‘marker-assisted’ breeding program of roughly 2–3 years in duration. To create a CSS for chromosome i, one starts with (A×B)F1 progeny and performs successive backcrosses to strain A (Fig. 2). ...
... for the strain combination of interest. For the mouse, a total of 21 strains must be constructed through a ‘marker-assisted’ breeding program of roughly 2–3 years in duration. To create a CSS for chromosome i, one starts with (A×B)F1 progeny and performs successive backcrosses to strain A (Fig. 2). ...
Genes for Cognitive Function: Developments on the X
... became instant candidates for familial XLMR, and they were independently confirmed as genes for XLMR by detection of mutations in families linked to the same regions. The three remaining genes, GDI1 (D’Adamo et al. 1998), PAK3 (Allen et al. 1998), and RPS6KA3 (Merienne et al. 1999), were identified ...
... became instant candidates for familial XLMR, and they were independently confirmed as genes for XLMR by detection of mutations in families linked to the same regions. The three remaining genes, GDI1 (D’Adamo et al. 1998), PAK3 (Allen et al. 1998), and RPS6KA3 (Merienne et al. 1999), were identified ...
Mycobacterium tuberculosis
... This Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) Gene Database was built on October 20, 2010; this build date reflected in the filename Mt-Std_External_20110303.gdb. All date fields internal to the Gene Database (and not usually seen by regular GenMAPP users) have been filled with this ...
... This Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) Gene Database was built on October 20, 2010; this build date reflected in the filename Mt-Std_External_20110303.gdb. All date fields internal to the Gene Database (and not usually seen by regular GenMAPP users) have been filled with this ...
Within-field Pathogenic Diversity of Phytophthora sojae in
... germplasm (3,16); however, only Rps1c, Rps1k, Rps3a, and Rps6 have been deployed in commercial soybean varieties in the north central region of the United States (8). In Iowa, Rps1k is the most common resistance gene used at present, followed by Rps1c, but the increased prevalence of race 25 (pathot ...
... germplasm (3,16); however, only Rps1c, Rps1k, Rps3a, and Rps6 have been deployed in commercial soybean varieties in the north central region of the United States (8). In Iowa, Rps1k is the most common resistance gene used at present, followed by Rps1c, but the increased prevalence of race 25 (pathot ...
Tutorial - Maize Inflorescence
... RNAseq: To know whether the selected genes are differentially expressed. ChIPseq: To query for presence of Peak summits within 2kb of the gene. SNPs: To know if there is any SNPs within 2kb of the gene. Orthologs: To get the Orthologs Save list: To save list of gene ids in a file ...
... RNAseq: To know whether the selected genes are differentially expressed. ChIPseq: To query for presence of Peak summits within 2kb of the gene. SNPs: To know if there is any SNPs within 2kb of the gene. Orthologs: To get the Orthologs Save list: To save list of gene ids in a file ...
Sample pages 1 PDF
... This is because the bacterial indicator cells possess a very limited capacity for endogenous metabolism of xenobiotics. Many carcinogens and mutagens are unable to interact with DNA unless they have undergone some degree of metabolism. To improve the ability of the bacterial test systems to detect a ...
... This is because the bacterial indicator cells possess a very limited capacity for endogenous metabolism of xenobiotics. Many carcinogens and mutagens are unable to interact with DNA unless they have undergone some degree of metabolism. To improve the ability of the bacterial test systems to detect a ...
Name that Gene Project The National Center for Biotechnology
... term. It then compares your character string (nucleotide sequence or protein sequence) against all the sequences in the target database. The program uses rigorous statistics to identify statistically significant matches. EXERCISE 1: From the main BLAST page select Nucleotide BLAST. This brings up a ...
... term. It then compares your character string (nucleotide sequence or protein sequence) against all the sequences in the target database. The program uses rigorous statistics to identify statistically significant matches. EXERCISE 1: From the main BLAST page select Nucleotide BLAST. This brings up a ...
Bioinfogrid_EGAAP
... • The BIOINFOGRID Initial training course has been opened to other bioinformatics national projects (≈ 25 participants) • Many thanks to EGEE for the really strong support. • We expect that the trained BIOINFOGRID personnel will join the Biomed VO after the course. • A second training course will be ...
... • The BIOINFOGRID Initial training course has been opened to other bioinformatics national projects (≈ 25 participants) • Many thanks to EGEE for the really strong support. • We expect that the trained BIOINFOGRID personnel will join the Biomed VO after the course. • A second training course will be ...
in silico PCR-RFLP of Bacillus species: a problem
... applications of molecular taxonomy in bacterial identification. Although the 16S rRNA gene sequence is well known to have highly conserved structures, it contains some variable regions that can be used as specific probes for detection and identification. Such sequences are useful and can be applied ...
... applications of molecular taxonomy in bacterial identification. Although the 16S rRNA gene sequence is well known to have highly conserved structures, it contains some variable regions that can be used as specific probes for detection and identification. Such sequences are useful and can be applied ...
Study of Hypertension in Spontaneous Hypertensive Rats by
... Results: There were no important differences between all the above rats in the 6,268 nucleotides of the alpha2B receptor. Conclusion: Our data suggest that, although the alpha2B adrenergic receptor has a crucial role in the development of hypertension, the genetic susceptibility or resistance of var ...
... Results: There were no important differences between all the above rats in the 6,268 nucleotides of the alpha2B receptor. Conclusion: Our data suggest that, although the alpha2B adrenergic receptor has a crucial role in the development of hypertension, the genetic susceptibility or resistance of var ...
2.4. Sequence databases
... relational databases e.g. it is not so easy to add additional data to the database, therefore the whole database structure needs to be updated. There is also a risk that some of the relationships between objects may be misrepresented. Some current databases have therefore incorporated features of bo ...
... relational databases e.g. it is not so easy to add additional data to the database, therefore the whole database structure needs to be updated. There is also a risk that some of the relationships between objects may be misrepresented. Some current databases have therefore incorporated features of bo ...
summary_Stickleback_Seg_Dup
... 92% of total intra chromosomal WGAC pairs and 81% total pairs has at least one sequence in the pair is on chrUn. The result is expected, since chrUn contains high percentage of redundant poorly assembled sequences. Our analysis also suggest that the potential repeats which are not covered by the rep ...
... 92% of total intra chromosomal WGAC pairs and 81% total pairs has at least one sequence in the pair is on chrUn. The result is expected, since chrUn contains high percentage of redundant poorly assembled sequences. Our analysis also suggest that the potential repeats which are not covered by the rep ...
TregouetD_EGEE3-presentation
... Estimate the SNP allele frequencies in cases and controls and calculate the corresponding statistical test yielding a pvalue • SNP definition Genetic variation in a DNA sequence that occurs when a single nucleotide (~ base: A,C,G,T ) in a genome is altered. Often considered as a binary 0/1 variable ...
... Estimate the SNP allele frequencies in cases and controls and calculate the corresponding statistical test yielding a pvalue • SNP definition Genetic variation in a DNA sequence that occurs when a single nucleotide (~ base: A,C,G,T ) in a genome is altered. Often considered as a binary 0/1 variable ...
mutant_tutorial
... section of Gramene that provides descriptions of alleles associated with morphological, developmental, and agronomically important phenotypes and variants of physiological characters, biochemical functions and isozymes. Many genes in Gramene also have map positions and sequencing data. ...
... section of Gramene that provides descriptions of alleles associated with morphological, developmental, and agronomically important phenotypes and variants of physiological characters, biochemical functions and isozymes. Many genes in Gramene also have map positions and sequencing data. ...
Temperature-dependent expression of virulence genes in fish
... the infection process of endothermic organisms by pathogenic bacteria. These bacteria have to adjust their physiology to the host temperature, which is usually higher than that encountered within a vector or in the natural environment. This accommodation may involve the induction of both virulence a ...
... the infection process of endothermic organisms by pathogenic bacteria. These bacteria have to adjust their physiology to the host temperature, which is usually higher than that encountered within a vector or in the natural environment. This accommodation may involve the induction of both virulence a ...
The relation of genetics to physiology and medicine
... cells. Here we appear to approach a physiological problem, but one that is new and strange to the classical physiology of the schools. We ascribe certain general properties to the genes, in part from genetic evidence and in part from microscopical observations. These properties we may next consider. ...
... cells. Here we appear to approach a physiological problem, but one that is new and strange to the classical physiology of the schools. We ascribe certain general properties to the genes, in part from genetic evidence and in part from microscopical observations. These properties we may next consider. ...
Clustering Gene Expression Data: The Good, The Bad, and The
... • “Clustering” is an exploratory tool for looking at associations within gene expression data • Hierarchical clustering dendrograms allow us to visualize gene expression data. • These methods allow us to hypothesize about relationships between genes and classes. • We should use these methods for vis ...
... • “Clustering” is an exploratory tool for looking at associations within gene expression data • Hierarchical clustering dendrograms allow us to visualize gene expression data. • These methods allow us to hypothesize about relationships between genes and classes. • We should use these methods for vis ...