Sequential Elimination of Major-Effect Contributors Identifies
... effects of major loci and epistasis (Flint et al. 2005). In a recent study, a two-stage search strategy was used to first map the major-effect QTL and then to partition segregants on the basis of genotype at this locus: each subgroup of segregants (sharing one allele at the first QTL) was then used ...
... effects of major loci and epistasis (Flint et al. 2005). In a recent study, a two-stage search strategy was used to first map the major-effect QTL and then to partition segregants on the basis of genotype at this locus: each subgroup of segregants (sharing one allele at the first QTL) was then used ...
A Study of Linkage in Haploid Budding Yeast by Random Spore
... 5. You will need isolated colonies that developed from a single cell to determine the genotype next week; therefore you need to spread the yeast cells for isolation onto YPD plates. Label the bottom of the plates, not the lids, with your initials, the date, lab section and dilution factor. Place 100 ...
... 5. You will need isolated colonies that developed from a single cell to determine the genotype next week; therefore you need to spread the yeast cells for isolation onto YPD plates. Label the bottom of the plates, not the lids, with your initials, the date, lab section and dilution factor. Place 100 ...
Methods S1: Vector constructions and transformation of yeast and
... AvrLm4-7 in tobacco leaves, alone or fused to eGFP. eGFP coding sequence was amplified from plasmid peGFP (Clontech, Mountain View, CA, USA) using primers pBINeGFPXbaUp (which introduces a XbaI restriction site) and pBINeGFP-SacLo (which introduces a SacI restriction site). eGFP PCR product was dige ...
... AvrLm4-7 in tobacco leaves, alone or fused to eGFP. eGFP coding sequence was amplified from plasmid peGFP (Clontech, Mountain View, CA, USA) using primers pBINeGFPXbaUp (which introduces a XbaI restriction site) and pBINeGFP-SacLo (which introduces a SacI restriction site). eGFP PCR product was dige ...
Fungal Genetics Newsletter 54 In Press David Dexter Perkins (1919-2007)
... incorporated three reciprocal chromosome rearrangements into a single strain “alcoy" for mapping various mutant genes. He was also the main driving force behind the Neurospora Methods Manual (ed. Deborah Bell-Pederson), which is available on line at the FGSC site. The Newsletter, like the whole Neur ...
... incorporated three reciprocal chromosome rearrangements into a single strain “alcoy" for mapping various mutant genes. He was also the main driving force behind the Neurospora Methods Manual (ed. Deborah Bell-Pederson), which is available on line at the FGSC site. The Newsletter, like the whole Neur ...
RTPrimerDB: the real-time PCR primer and probe database, major
... annotation data and links to Entrez Gene and Ensembl, followed by a graphical representation of the aligned primer and probe sequences onto the different transcript variants available from Ensembl. The occurrence of SNPs in the region where the primer/probe anneals and the exact location of the ampl ...
... annotation data and links to Entrez Gene and Ensembl, followed by a graphical representation of the aligned primer and probe sequences onto the different transcript variants available from Ensembl. The occurrence of SNPs in the region where the primer/probe anneals and the exact location of the ampl ...
Extremophiles - University of Arizona
... such as those found in Egypt, the Rift the halophiles—makes its home in in- tremozymes. Nevertheless, at least one Valley of Africa and the western U.S. tensely saline environments, especially intriguing application is under considAbove a pH of eight or so, certain mol- natural salt lakes and solar ...
... such as those found in Egypt, the Rift the halophiles—makes its home in in- tremozymes. Nevertheless, at least one Valley of Africa and the western U.S. tensely saline environments, especially intriguing application is under considAbove a pH of eight or so, certain mol- natural salt lakes and solar ...
S1-1-13 - The Big Gamble - Lesson
... adults know that genes are responsible for the similarities between parents and their children, but cannot explain why (Driver et al, 2002). Please see the section “Learner Difficulties” for further information regarding student preconceptions in the field of inheritance. Students can be encouraged ...
... adults know that genes are responsible for the similarities between parents and their children, but cannot explain why (Driver et al, 2002). Please see the section “Learner Difficulties” for further information regarding student preconceptions in the field of inheritance. Students can be encouraged ...
Concepts and relevance of genome
... These common mutations are generally called variants or polymorphisms. The most common type of variants is the single-nucleotide polymorphism (SNP) which describes changes to an individual DNA base. The different forms of the same gene containing variable SNPs within the same site(s) are typically c ...
... These common mutations are generally called variants or polymorphisms. The most common type of variants is the single-nucleotide polymorphism (SNP) which describes changes to an individual DNA base. The different forms of the same gene containing variable SNPs within the same site(s) are typically c ...
Masters_Thesis_Final - JScholarship
... for many known complex traits and are capable of causing diseases [5]. These mutations lie in regulatory regions and affect gene expression levels. Hence, it is important to identify parts of genomes which act as regulators. Different regulatory elements may be surveyed in different applications, so ...
... for many known complex traits and are capable of causing diseases [5]. These mutations lie in regulatory regions and affect gene expression levels. Hence, it is important to identify parts of genomes which act as regulators. Different regulatory elements may be surveyed in different applications, so ...
letter Widespread aneuploidy revealed by DNA microarray expression profiling
... location, we noted an expres- Fig. 3 Selection for aneuploidy in rnr1∆ and rps24a∆/rps24a∆ mutants. a, Slow-growing rnr1∆ (sector 2) or sion bias in a 56-ORF region on rps24a∆/rps24a∆ (sector 4) cells or their isogenic parental wild-type cells (sectors 1 and 3) were streaked onto solid the right arm ...
... location, we noted an expres- Fig. 3 Selection for aneuploidy in rnr1∆ and rps24a∆/rps24a∆ mutants. a, Slow-growing rnr1∆ (sector 2) or sion bias in a 56-ORF region on rps24a∆/rps24a∆ (sector 4) cells or their isogenic parental wild-type cells (sectors 1 and 3) were streaked onto solid the right arm ...
Bacteria are different: Observations, interpretations
... is through genes borne on, transmitted by, and sequestered from these elements. And, from this perspective, the population and evolutionary dynamics of these elements form an integral part of the process of adaptive evolution in bacteria. Variations on a Single Theme. Traditionally, we classify the ...
... is through genes borne on, transmitted by, and sequestered from these elements. And, from this perspective, the population and evolutionary dynamics of these elements form an integral part of the process of adaptive evolution in bacteria. Variations on a Single Theme. Traditionally, we classify the ...
Gene Section IGH@ (Immunoglobulin Heavy) Atlas of Genetics and Cytogenetics
... IGHC genes. Eighty-two to 88 IGHV genes belong to 7 subgroups, whereas 41 pseudogenes, which are too divergent to be assigned to subgroups, have been assigned to 4 clans. Seven non-mapped IGHV genes have been described as insertion/deletion polymorphism but have not yet been precisely located. The m ...
... IGHC genes. Eighty-two to 88 IGHV genes belong to 7 subgroups, whereas 41 pseudogenes, which are too divergent to be assigned to subgroups, have been assigned to 4 clans. Seven non-mapped IGHV genes have been described as insertion/deletion polymorphism but have not yet been precisely located. The m ...
Pair-wise sequence alignment
... • Paralogous sequences are homologous sequences that exists within a species • They have a common origin but involve gene duplication events to arise • Purpose of gene duplication is to use sequence to implement a new function • Perform different functions ...
... • Paralogous sequences are homologous sequences that exists within a species • They have a common origin but involve gene duplication events to arise • Purpose of gene duplication is to use sequence to implement a new function • Perform different functions ...
Evolution of cis-regulatory elements in duplicated genes of yeast
... due to the cellular concentration of its substrate but instead due to changes in another function, such as altered interaction with another pathway, identification of the other function or pathway might lead to a method to treat the tumors without having to replace the fumarate hydratase protein. It ...
... due to the cellular concentration of its substrate but instead due to changes in another function, such as altered interaction with another pathway, identification of the other function or pathway might lead to a method to treat the tumors without having to replace the fumarate hydratase protein. It ...
Identification and quantification of mycotoxigenic fungi
... It is common to analyse pure cultures of fungi by PCR. This is often undertaken for taxonomic, or more specifically phylogenic, purposes [2]. However, in a review such as this it is sometimes necessary to emphasise the potential pitfalls: two bands of equal size on a gel may not represent the same D ...
... It is common to analyse pure cultures of fungi by PCR. This is often undertaken for taxonomic, or more specifically phylogenic, purposes [2]. However, in a review such as this it is sometimes necessary to emphasise the potential pitfalls: two bands of equal size on a gel may not represent the same D ...
Word - NIEHS SNPs Program - University of Washington
... As a launching point, we will begin our search at the NIEHS SNPs resource. This can be accessed at http://egp.gs.washington.edu/ The NIEHS SNPs Program at the University of Washington is part of the EGP. The EGP is a multi-disciplinary effort focused on exploring the relationships between environmen ...
... As a launching point, we will begin our search at the NIEHS SNPs resource. This can be accessed at http://egp.gs.washington.edu/ The NIEHS SNPs Program at the University of Washington is part of the EGP. The EGP is a multi-disciplinary effort focused on exploring the relationships between environmen ...
Preimplantation Genetic Testing An Overview
... Ratio of the fluorescence intensities is proportional to the ratio of 'copy number of DNA sequences' in the test and reference genomes. Altered Cy 3 : Cy 5 ratio indicates a loss or gain of the patient DNA at that specific genomic region If Cy 3 : Cy 5 ratio is equal on one probe (equal intens ...
... Ratio of the fluorescence intensities is proportional to the ratio of 'copy number of DNA sequences' in the test and reference genomes. Altered Cy 3 : Cy 5 ratio indicates a loss or gain of the patient DNA at that specific genomic region If Cy 3 : Cy 5 ratio is equal on one probe (equal intens ...
STM
... 20-oxidase1 (GA20ox1) and the catabolic gene GA 2-oxidase1 (GA2ox1) to reduce gibberellin (GA) levels, and the lignin biosynthetic genes caffeic acid-O-methyltransferase1 (COMT1), caffeoyl-CoA O-methyltransferase (CCoAOMT) and Arabidopsis thaliana peroxidase12a (AtP12a) to reduce lignin levels. KNOX ...
... 20-oxidase1 (GA20ox1) and the catabolic gene GA 2-oxidase1 (GA2ox1) to reduce gibberellin (GA) levels, and the lignin biosynthetic genes caffeic acid-O-methyltransferase1 (COMT1), caffeoyl-CoA O-methyltransferase (CCoAOMT) and Arabidopsis thaliana peroxidase12a (AtP12a) to reduce lignin levels. KNOX ...
Integrated genome sequence and linkage map of physic nut
... and 0.746 Mbp, respectively, which are approximately 1.8 and 46.8 times longer, respectively, than the reported physic nut genome (Hirakawa et al., 2012). As the result of short contigs and scaffolds, too many predicted genes in their study were fragmented. Only 17 Mbp (6%) of their assembly (but ab ...
... and 0.746 Mbp, respectively, which are approximately 1.8 and 46.8 times longer, respectively, than the reported physic nut genome (Hirakawa et al., 2012). As the result of short contigs and scaffolds, too many predicted genes in their study were fragmented. Only 17 Mbp (6%) of their assembly (but ab ...
Groups Basics - Bioinformatics Research Group at SRI International
... We have a file containing replicon coordinates here. [show file regions.txt] You can see replicon names, start, and end positions for each region. Note that start positions have a larger value than end positions to represent a region that has a reverse direction. Let’s upload the file now. And now w ...
... We have a file containing replicon coordinates here. [show file regions.txt] You can see replicon names, start, and end positions for each region. Note that start positions have a larger value than end positions to represent a region that has a reverse direction. Let’s upload the file now. And now w ...
embr201439791-sup-0014
... the transcriptome, spun down in PBS for 5 min at 7500 rpm and immediately frozen in 100 µl TriPure reagent (#11667157001, Roche) on dry ice. To isolate RNA, samples were thawed, homogenized with a blue centrifuge pestle, combined so each sample contained ~100-150 flies, incubated at RT for 10 min an ...
... the transcriptome, spun down in PBS for 5 min at 7500 rpm and immediately frozen in 100 µl TriPure reagent (#11667157001, Roche) on dry ice. To isolate RNA, samples were thawed, homogenized with a blue centrifuge pestle, combined so each sample contained ~100-150 flies, incubated at RT for 10 min an ...
Potato Genome Sequencing Consortium: Final
... Copyright, Agriculture and Horticulture Development Board 2012. No part of this publication may be reproduced in any material form (including by photocopy or storage in any medium by electronic means) or any copy or adaptation stored, published or distributed (by physical, electronic or other means) ...
... Copyright, Agriculture and Horticulture Development Board 2012. No part of this publication may be reproduced in any material form (including by photocopy or storage in any medium by electronic means) or any copy or adaptation stored, published or distributed (by physical, electronic or other means) ...
ª2010 Elsevier Ltd All rights reserved DOI 10.1016/j.cub.2010.06.022
... ecological isolation [3, 4] and Dobzhansky-Muller (DM) interaction [5]. Under ecological isolation, populations adapt to divergent environments through the accumulation of genetic changes that result in increased fitness. If formed, hybrid populations are genotypically intermediate and therefore sub ...
... ecological isolation [3, 4] and Dobzhansky-Muller (DM) interaction [5]. Under ecological isolation, populations adapt to divergent environments through the accumulation of genetic changes that result in increased fitness. If formed, hybrid populations are genotypically intermediate and therefore sub ...
GENETICS – BIO 300
... gene evolution ... relative positions of genes in related organisms infer history of change ...
... gene evolution ... relative positions of genes in related organisms infer history of change ...