1 Chromosome Mapping in Eukaryotes
... – Identify the single allele that has been switched so that it no longer is associated with its original neighboring alleles. – This allele is in the middle. ...
... – Identify the single allele that has been switched so that it no longer is associated with its original neighboring alleles. – This allele is in the middle. ...
An rpoB signature sequence provides unique resolution for the
... The use of morphological characters for the classification of cyanobacteria has often led to ambiguous strain assignment. In the past two decades, the availability of sequences, such as those of the 16S rRNA, nif, cpc and rpoC1 genes, and the use of metagenomics, has steadily increased and has made ...
... The use of morphological characters for the classification of cyanobacteria has often led to ambiguous strain assignment. In the past two decades, the availability of sequences, such as those of the 16S rRNA, nif, cpc and rpoC1 genes, and the use of metagenomics, has steadily increased and has made ...
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nature | methods Versatile P[acman] BAC libraries for transgenesis
... DNA, isolated from the isogenized y1; cn1 bw1 sp1 strain, was partially digested with MboI and ligated into the BamHI site of the attB-P(acman)-CmR-BW plasmid. White recombinant clones ...
... DNA, isolated from the isogenized y1; cn1 bw1 sp1 strain, was partially digested with MboI and ligated into the BamHI site of the attB-P(acman)-CmR-BW plasmid. White recombinant clones ...
Microbial Ecology: Where are we now?
... begin with molecular approaches on bacterial characterization (16S rRNA, DGGE, T-RFLP) and a section on typing bacterial pathogens. Followed by the various NGS technologies currently used (targeted amplification, metagenomics, RNA-seq, proteomics, Tn-seq). Included in each section are selected examp ...
... begin with molecular approaches on bacterial characterization (16S rRNA, DGGE, T-RFLP) and a section on typing bacterial pathogens. Followed by the various NGS technologies currently used (targeted amplification, metagenomics, RNA-seq, proteomics, Tn-seq). Included in each section are selected examp ...
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... and unavoidable, resulting in the problem that the raw measurements have inherent “noise” within microarray experiments. Currently, logarithmic ratios are usually analyzed by various clustering methods directly, which may introduce bias interpretation in identifying groups of genes or samples. In th ...
... and unavoidable, resulting in the problem that the raw measurements have inherent “noise” within microarray experiments. Currently, logarithmic ratios are usually analyzed by various clustering methods directly, which may introduce bias interpretation in identifying groups of genes or samples. In th ...
Anopheles gambiae APL1 Is a Family of Variable LRR Proteins
... for stable haplotypes that encode predicted proteins of distinct sizes and structure. The haplotypes are designated by the gene name followed by a superscript number (Figure 1B). The superscript 2 haplotype for each gene is most similar to the variant found in the PEST strain used for the A. gambiae ...
... for stable haplotypes that encode predicted proteins of distinct sizes and structure. The haplotypes are designated by the gene name followed by a superscript number (Figure 1B). The superscript 2 haplotype for each gene is most similar to the variant found in the PEST strain used for the A. gambiae ...
Quantitative genetics and breeding theory
... Status number is the size of unrelated non inbred trees sampled from the reference population, which have the same drift as the accumulated drift of the population under study (compared to the reference population). Gene frequency ...
... Status number is the size of unrelated non inbred trees sampled from the reference population, which have the same drift as the accumulated drift of the population under study (compared to the reference population). Gene frequency ...
Historical Development of the Concept of the Gene
... heterozygotes are compared. In the cis-heterozygote the mutations are in the same chromosome, but in the trans-heterozygote they are in homologous chromosomes. Thus, the genotype of the cis-heterozygote is designated as a b/, and that of the trans-heterozygote as a/b. If the cis-heterozygote is ...
... heterozygotes are compared. In the cis-heterozygote the mutations are in the same chromosome, but in the trans-heterozygote they are in homologous chromosomes. Thus, the genotype of the cis-heterozygote is designated as a b/, and that of the trans-heterozygote as a/b. If the cis-heterozygote is ...
Recent Statistical Approaches
... 1) Form a statistic tj for each gene, where a large positive value of tj corresponds to a gene that is differentially expressed across the tissues. 2) Compute the Pj-values according to the tj and fit a mixture of beta distributions (including a uniform component) to them where the latter correspon ...
... 1) Form a statistic tj for each gene, where a large positive value of tj corresponds to a gene that is differentially expressed across the tissues. 2) Compute the Pj-values according to the tj and fit a mixture of beta distributions (including a uniform component) to them where the latter correspon ...
The evolutionary mechanics of bacterial protein toxins
... perform the same pathogenic action through the same receptor. They have one A subunit that stops protein synthesis inside cells, and 5 identical B subunits that help get the A subunit into the cell. These toxins are similar in structure and function to the plant toxin ricin. These toxins cause b ...
... perform the same pathogenic action through the same receptor. They have one A subunit that stops protein synthesis inside cells, and 5 identical B subunits that help get the A subunit into the cell. These toxins are similar in structure and function to the plant toxin ricin. These toxins cause b ...
Investigating the molecular mechanisms behind flowering in
... AP1 homologs have been identified (Nakagawa et al., 2012, Hu et al., 2003, Davenport et al., 2006a, Luo et al., 2009, de los Santos-Villalobos et al., 2012). At present the MiFT genes is the strongest candidate for the genetic precursor of the mango FP, it’s expression increases in response to cooli ...
... AP1 homologs have been identified (Nakagawa et al., 2012, Hu et al., 2003, Davenport et al., 2006a, Luo et al., 2009, de los Santos-Villalobos et al., 2012). At present the MiFT genes is the strongest candidate for the genetic precursor of the mango FP, it’s expression increases in response to cooli ...
Ecological Risks of Gene Drive Technologies
... ● Build local rather than global drives - for example, elements which cannot spread unless they exceed a threshold frequency in the population, or multi-part genetic designs that drive initially then decay away and so have limited time to spread; ● Reversibility – before a modification gene drive sy ...
... ● Build local rather than global drives - for example, elements which cannot spread unless they exceed a threshold frequency in the population, or multi-part genetic designs that drive initially then decay away and so have limited time to spread; ● Reversibility – before a modification gene drive sy ...
A general method for gene isolation in tagging approaches
... fragments larger than 500 base pairs is difficult since artefacts of uncut DNA might be displayed, or the fragments might not always be amplified thoroughly by the Taq polymerase. Premature termination of DNA synthesis leads to an accumulation of small-sized bands (less than 80 bp) which also makes ...
... fragments larger than 500 base pairs is difficult since artefacts of uncut DNA might be displayed, or the fragments might not always be amplified thoroughly by the Taq polymerase. Premature termination of DNA synthesis leads to an accumulation of small-sized bands (less than 80 bp) which also makes ...
Bioinformatics Course Notes (Ming Li)
... -db: multiple sequence input, 0,1,2,3 (no, query, subject, both) -W: seed weight ...
... -db: multiple sequence input, 0,1,2,3 (no, query, subject, both) -W: seed weight ...
ppt - Chair of Computational Biology
... This suggests that many TFs can be regulated simply by the abundance (expression levels) of the TF. However, across 1000 microarray expression experiments for yeast, the correlation between a TF’s expression and that of its ChIP-based targets was typically very low (only between 0 and 0.25). Conside ...
... This suggests that many TFs can be regulated simply by the abundance (expression levels) of the TF. However, across 1000 microarray expression experiments for yeast, the correlation between a TF’s expression and that of its ChIP-based targets was typically very low (only between 0 and 0.25). Conside ...
Document
... the computational platform developed in and utilized by Dr. dos Santos’ laboratory for constraint based modeling applications. Any unique code for each reaction stoichiometry would work equally well. The key used to refer to genes was the gene locus ID. The tables described in Figure 2 in Text S2 we ...
... the computational platform developed in and utilized by Dr. dos Santos’ laboratory for constraint based modeling applications. Any unique code for each reaction stoichiometry would work equally well. The key used to refer to genes was the gene locus ID. The tables described in Figure 2 in Text S2 we ...
GENES AND SPECIATION
... ‘weak effect–strong interaction’ — that is, each individual gene has little effect on its own, but in combination, these genes cause reproductive incompatibility10. Functional studies of one of these interacting genes (OdsH; see below) have indeed shown that it has an extremely weak effect. Despite ...
... ‘weak effect–strong interaction’ — that is, each individual gene has little effect on its own, but in combination, these genes cause reproductive incompatibility10. Functional studies of one of these interacting genes (OdsH; see below) have indeed shown that it has an extremely weak effect. Despite ...
Differential Regulation of Antagonistic Pleiotropy in Synthetic and
... phenotypes, has been identified in various life history traits and complex disorders, indicating ...
... phenotypes, has been identified in various life history traits and complex disorders, indicating ...
Methods S1: Vector constructions and transformation of yeast and
... AvrLm4-7 in tobacco leaves, alone or fused to eGFP. eGFP coding sequence was amplified from plasmid peGFP (Clontech, Mountain View, CA, USA) using primers pBINeGFPXbaUp (which introduces a XbaI restriction site) and pBINeGFP-SacLo (which introduces a SacI restriction site). eGFP PCR product was dige ...
... AvrLm4-7 in tobacco leaves, alone or fused to eGFP. eGFP coding sequence was amplified from plasmid peGFP (Clontech, Mountain View, CA, USA) using primers pBINeGFPXbaUp (which introduces a XbaI restriction site) and pBINeGFP-SacLo (which introduces a SacI restriction site). eGFP PCR product was dige ...