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How to determine whether a strain will undergo senescence.  Background
How to determine whether a strain will undergo senescence. Background

... accumulate and slow or terminate growth even in normal growing, nonsenescent cultures. Occasional outcrossing can purge the genome of the accumulated defective genes, but in the absence of genetic recombination, deleterious mutations are expected ultimately to result in death of a serially propogate ...
Altering gene expression by aminocoumarins: the role of DNA
Altering gene expression by aminocoumarins: the role of DNA

... We previously showed that different aminocoumarins cause severe inhibition of recA transcription [20]. This effect is likely mediated by the known inhibition of the GyrB subunit. To verify this assumption, a strain expressing a non-susceptible GyrB enzyme was analysed. A mutated gyrB gene (Ile102Ser ...
The Big Picture: an outline of the concepts covered to date
The Big Picture: an outline of the concepts covered to date

... Up until this point, genes have been viewed as a linear array of indivisible functional units on a chromosome ...
Exploring HLA Diversity in Brazil
Exploring HLA Diversity in Brazil

... gene produces an immune tolerance protein that is active at the fetal/maternal interface. We were trying to understand the genetics of HLA-G. It is quite different from the other HLA genes because it is highly conserved. Our main focus was on the population genetics around this gene. As we learned m ...
Evidence That the Human X Chromosome Is Enriched for Male
Evidence That the Human X Chromosome Is Enriched for Male

... represents a significant enrichment (6.5/ 7.3 are expected, P ¼ 0.02, one tailed, derived by 100,000 randomizations). Pairwise Blast of all of the X-linked prostate-specific genes against all the others on the X chromosome revealed no duplicate genes, so the enrichment is not owing to higher rates o ...
Exam 3 ANSWER KEY Page 1 [10] 1. The proBA genes are required
Exam 3 ANSWER KEY Page 1 [10] 1. The proBA genes are required

... fusaric acid the putP gene results in sensitivity to toxic proline analogs so you could select for insertions that disrupt this gene by demanding growth on plates containing azetidine carboxylic acid or dehydroproline; etc. A straightforward approach for selecting for transposition would be to elect ...
Candidatus Mycoplasma haemomuris subsp. ratti
Candidatus Mycoplasma haemomuris subsp. ratti

... 2005; Rikihisa et al., 1997). Recent studies have suggested that M. haemomuris consists of two subgroups based on differences in the 16S–23S rRNA intergenic spacer (ITS) The GenBank/EMBL/DDBJ accession numbers for rnpB and dnaK, appearing for the first time in this study, are AB973078 to AB973092. T ...
FEATURE SELECTION = GENE SELECTION
FEATURE SELECTION = GENE SELECTION

File S1
File S1

... Supplementary Figure 2. Expression profile graphs for the genes that are differentially expressed in all four brain region. The 49 genes’ expression values (average FPKM across the replicates) were plotted. The Y-axis depicts the range of the average FPKM values, and each colored line represents a g ...
Evolution of Immunoglobulin Kappa Chain Variable Region
Evolution of Immunoglobulin Kappa Chain Variable Region

... overall sequence divergence between the two sets of duplicate genes (;1%), Schäble and Zachau (1993) suggested that the duplication occurred about 1 MYA. The copy of the Vk region adjacent to Jk genes is called the p (proximal) contig, whereas the other copy of this region is called the d (distal) ...
Woolfe, 2005
Woolfe, 2005

... Experimentally showed CNE-transdev gene association CNEs found in clusters, in front of transdev genes CNEs act at large distances from coding sequence The relative order and positions of CNEs are conserved No vertebrate CNEs were found in invertebrates, even though the genes had clear homologs Many ...
PDF Full-text
PDF Full-text

... We then investigated the collected TyrRS sequences. A high-resolution phylogeny for archaea/ eukarya-type TyrRS suggests that several lineages of CPR bacteria independently obtained an archaea/eukarya-type TyrRS gene from archaea (Figure 2). Alternatively, lateral gene transfer (LGT) might have occu ...
9 The Secretome of Plant-Associated Fungi and Oomycetes
9 The Secretome of Plant-Associated Fungi and Oomycetes

Determination of guanine-plus-cytosine content of
Determination of guanine-plus-cytosine content of

... A dual-laser flow cytometer was used to analyse different species of bacteria for the molar percentage of guanineplus-cytosine (% G + C) without the need for DNA extraction or purification. Ethanol-fixed bacterial cells were stained with a combination of DNA-specific fluorochromes, Hoechst 33258 and ...
A Comparative Gene Map of the Horse (Equus caballus)
A Comparative Gene Map of the Horse (Equus caballus)

... Andersson et al. 1996; Wakefield and Graves 1996). This information forms the basis for comparative genome mapping, a discipline that allows the prospect of using information from highly characterized genomes to study genetic phenomena in map-poor species (O’Brien et al. 1993; Georges and Andersson ...
Jane Yeadon  How to do recombination assays.
Jane Yeadon How to do recombination assays.

... or between two genes in a particular chromosomal interval, which is a measure of crossing over. Allelic assays require a cross that is heteroallelic, for example, between two strains each with a different his-3 mutation. Non-allelic assays are between strains that have mutations in two distinct gene ...
Array Flip Book
Array Flip Book

... GenomeDx Oligonucleotide Array Comparative Genomic Hybridization (Oligo aCGH) • GenomeDx is a diagnostic test, offered through GeneDx, that can identify regions of gain or loss of genetic material across the entire human genome (with the exception of centromeres, telomeres, and satellites) • GenomeD ...
Genetic and epigenetic dissection of cis regulatory
Genetic and epigenetic dissection of cis regulatory

... target DNA fragments using antibodies against 5-methyl cytosine. When combined with microarray technology, these two approaches have been used successfully in global DNA methylation profiling [39–42]. The first high-density genomic methylation map for Arabidopsis was recently published using ChIP fo ...
1/19/2016 1 The Effect of Artificial Selection on Phenotypic Plasticity
1/19/2016 1 The Effect of Artificial Selection on Phenotypic Plasticity

Characterization of an IS-like element from
Characterization of an IS-like element from

Document
Document

... In fact, we can define one genetic map unit (m.u.) as that distance between genes for which one product of meiosis in 100 is recombinant. Put another way, a recombinant frequency (RF) of 0.01 (1 percent) is defined as 1 m.u A map unit is today referred to as a centimorgan (cM) in honor of Morgan A d ...
On the internal dynamics of mendelian genetics
On the internal dynamics of mendelian genetics

CGC07OthrGene - Cucurbit Breeding
CGC07OthrGene - Cucurbit Breeding

... cucumber, melon and watermelon. Those are major crop species originally from the Old World: cucumber from India; melon and watermelon from Africa (Wehner and Maynard, 2003). However, there are other important species originally from Africa such as gherkin (Cucumis anguria), African horned melon (Cuc ...
Characterization of lysine decarboxylase
Characterization of lysine decarboxylase

Alpha Thalassemia - Lab Test Directory
Alpha Thalassemia - Lab Test Directory

... o Individual is predicted to be affected with Hb H disease • Predicted genotype (--/--) o Result is consistent with Hb Bart hydrops fetalis syndrome • Predicted genotype (ααα/αα) o An extra functional α-globin gene present Deletion or duplication of unknown clinical significance detected • Breakpoin ...
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Pathogenomics

Pathogen infections are among the leading causes of infirmity and mortality among humans and other animals in the world. Until recently, it has been difficult to compile information to understand the generation of pathogen virulence factors as well as pathogen behaviour in a host environment. The study of Pathogenomics attempts to utilize genomic and metagenomics data gathered from high through-put technologies (e.g. sequencing or DNA microarrays), to understand microbe diversity and interaction as well as host-microbe interactions involved in disease states. The bulk of pathogenomics research concerns itself with pathogens that affect human health; however, studies also exist for plant and animal infecting microbes.
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