Exceptionally high levels of recombination
... [Supplemental material is available online at www.genome.org. The sequence data from this study have been submitted to dbGSS under accession nos. 15028937–15029063.] Sex is believed to have evolved as a mechanism to break apart and recombine genetic material (Michod and Levin 1988). The exchange of ...
... [Supplemental material is available online at www.genome.org. The sequence data from this study have been submitted to dbGSS under accession nos. 15028937–15029063.] Sex is believed to have evolved as a mechanism to break apart and recombine genetic material (Michod and Levin 1988). The exchange of ...
Chapter 3
... for animals from the third bilaterian clade, the lophotrochozoans. Therefore, we decided to compare genes known to be involved in mesoderm formation in deuterostomes and ecdysozoans with orthologs of those genes in lophotrochozoans, in particular in molluscs. An obvious candidate gene for a comparis ...
... for animals from the third bilaterian clade, the lophotrochozoans. Therefore, we decided to compare genes known to be involved in mesoderm formation in deuterostomes and ecdysozoans with orthologs of those genes in lophotrochozoans, in particular in molluscs. An obvious candidate gene for a comparis ...
A PCR approach to determine the distribution of toxin genes in
... As far as we can ascertain few, if any, of the so-called C. novyi strains had been tested in our laboratory specifically for the a toxin when originally identified, although some guinea pig inoculations had been performed in the 1970s but records are sketchy. However, all gave biochemical profiles – ...
... As far as we can ascertain few, if any, of the so-called C. novyi strains had been tested in our laboratory specifically for the a toxin when originally identified, although some guinea pig inoculations had been performed in the 1970s but records are sketchy. However, all gave biochemical profiles – ...
Genome partitioning of genetic variation for complex traits using
... highly polygenic traits, and that the additive variation explained by a part of the genome is approximately proportional to the total length of DNA contained within genes therein. Genome-wide association studies (GWAS) have led to the discovery of hundreds of marker loci that are associated with co ...
... highly polygenic traits, and that the additive variation explained by a part of the genome is approximately proportional to the total length of DNA contained within genes therein. Genome-wide association studies (GWAS) have led to the discovery of hundreds of marker loci that are associated with co ...
Gene targeting: vector design and construction
... your gene of interest? – Degree of homology may depend on the gene. – look at the genes surrounding your gene. If you find synteny, it is OK. • Which chromosome? Three copies of chromosome #2; single copy of chromosome Z in DT40, which was derived from female chicken. • Analyze/map genomic structure ...
... your gene of interest? – Degree of homology may depend on the gene. – look at the genes surrounding your gene. If you find synteny, it is OK. • Which chromosome? Three copies of chromosome #2; single copy of chromosome Z in DT40, which was derived from female chicken. • Analyze/map genomic structure ...
TEXT Definition Chromosomal alterations are variations from the
... been translocated to another location in the genome. Small tandem duplications, in which duplicate segments lie adjacent to one another, occur frequently in complex organisms. Tandem duplications are able to produce even more copies of the duplicated region by means of a process called unequal cross ...
... been translocated to another location in the genome. Small tandem duplications, in which duplicate segments lie adjacent to one another, occur frequently in complex organisms. Tandem duplications are able to produce even more copies of the duplicated region by means of a process called unequal cross ...
Evolution of meiosis genes in sexual vs. asexual Potamopyrgus
... unanswered questions in evolutionary biology. While these questions have been addressed to some extent in different asexual species, the most powerful tests of how sex and its absence influence evolution requires direct comparisons between closely related and otherwise similar sexual and asexual tax ...
... unanswered questions in evolutionary biology. While these questions have been addressed to some extent in different asexual species, the most powerful tests of how sex and its absence influence evolution requires direct comparisons between closely related and otherwise similar sexual and asexual tax ...
Molecular Evolution of the Endosperm Starch Synthesis Pathway
... Only samples with less than 50-bp missing data were included in the analyses. The missing data are due to the heterozygosity of indels in some samples or to various other reasons during the sequencing process. Therefore, slightly different sample set were used for different starch genes. Statistics ...
... Only samples with less than 50-bp missing data were included in the analyses. The missing data are due to the heterozygosity of indels in some samples or to various other reasons during the sequencing process. Therefore, slightly different sample set were used for different starch genes. Statistics ...
An accessible database for mouse and human whole transcriptome
... To use the database via the UCSC genome browser, follow these steps: (1) In the browser, select organism: ‘human’ or ‘mouse’. (2) Browse to your gene of interest. (3) To view the track ‘Whole transcriptome qPCR primers’ scroll down the page to the panel of suggested tracks named ‘Expression’. Turn t ...
... To use the database via the UCSC genome browser, follow these steps: (1) In the browser, select organism: ‘human’ or ‘mouse’. (2) Browse to your gene of interest. (3) To view the track ‘Whole transcriptome qPCR primers’ scroll down the page to the panel of suggested tracks named ‘Expression’. Turn t ...
Methicillin resistant Staphylococcus aureus (MRSA)
... surgical Glue 2 (Sr GEM I-Italy). The animals returned to individual cages and were examined every six hours. After 72 hours of observation, the euthanasia of the animals was performed by an overdose of isoflurane anesthetic (Forane® Abbott). All catheter s, heart, spleen and left kidney were explan ...
... surgical Glue 2 (Sr GEM I-Italy). The animals returned to individual cages and were examined every six hours. After 72 hours of observation, the euthanasia of the animals was performed by an overdose of isoflurane anesthetic (Forane® Abbott). All catheter s, heart, spleen and left kidney were explan ...
Different susceptibility of two animal species infected with isogenic
... D. M. Collins and others assessment of virulence in early work was based on the presence of visible lesions of tuberculosis in the spleen and, in later comparative work, on the enumeration of M. bovis c.f.u. in samples from the spleen. All animals were tested for infection by determining their dela ...
... D. M. Collins and others assessment of virulence in early work was based on the presence of visible lesions of tuberculosis in the spleen and, in later comparative work, on the enumeration of M. bovis c.f.u. in samples from the spleen. All animals were tested for infection by determining their dela ...
Optimizing Restriction Site Placement for Synthetic
... recognition site or restriction site. Unique restriction sites within a given target are particularly prized, as they cut the sequence unambiguously in exactly one place. Many techniques for manipulating DNA make use of unique restriction sites [6, 7]. In particular, subcloning is an important metho ...
... recognition site or restriction site. Unique restriction sites within a given target are particularly prized, as they cut the sequence unambiguously in exactly one place. Many techniques for manipulating DNA make use of unique restriction sites [6, 7]. In particular, subcloning is an important metho ...
Supplementary Materials and methods (doc 154K)
... on LB agar containing the appropriate antibiotics (neomycin 50 mg/L, kanamycin 1200 mg/L, gentamycin 128 mg/L and chloramphenicol 50 mg/L), donors were selected on LB agar with neomycin (50 mg/L) and kanamycin (1200 mg/L). ...
... on LB agar containing the appropriate antibiotics (neomycin 50 mg/L, kanamycin 1200 mg/L, gentamycin 128 mg/L and chloramphenicol 50 mg/L), donors were selected on LB agar with neomycin (50 mg/L) and kanamycin (1200 mg/L). ...
Introductory genetics for veterinary students
... Is it reasonable to assume that the Q and q alleles will be alternatively “fixed” in the alternate F0 lines? Hence, that the QTL genotype of all F1 animals is the same? Especially when this is not the case for the SNP or microsatellite markers. This has lead to many erroneous conclusions of impr ...
... Is it reasonable to assume that the Q and q alleles will be alternatively “fixed” in the alternate F0 lines? Hence, that the QTL genotype of all F1 animals is the same? Especially when this is not the case for the SNP or microsatellite markers. This has lead to many erroneous conclusions of impr ...
Transcriptional analysis of the gene for glutamine synthetase II and
... in the glnII region of the S. viridochromogenes chromosome, this was not the case for orf3 and orf4, which encode a putative hydrolase and a transcriptional regulator (Ptr) of the MarR family, respectively. High-resolution S1 nuclease mapping showed that the S. coelicolor glnII gene is expressed fro ...
... in the glnII region of the S. viridochromogenes chromosome, this was not the case for orf3 and orf4, which encode a putative hydrolase and a transcriptional regulator (Ptr) of the MarR family, respectively. High-resolution S1 nuclease mapping showed that the S. coelicolor glnII gene is expressed fro ...
First report of a tetracycline-inducible gene
... Nevertheless, these operons are still expressed at a basal level in the absence of added sugars, indicating that they are not strongly repressed. Indeed, regulator FruR was shown to act as an activator of the fructose operon (Gaurivaud et al., 2001) and no repressor was identified. A variety of regu ...
... Nevertheless, these operons are still expressed at a basal level in the absence of added sugars, indicating that they are not strongly repressed. Indeed, regulator FruR was shown to act as an activator of the fructose operon (Gaurivaud et al., 2001) and no repressor was identified. A variety of regu ...
5 CHAPTER 2: LITERATURE REVIEW 2.1 Types of Ribonucleic
... structure in the pri-miRNA will be cleaved by RNase-III-type enzyme Dicer and nuclear microprocessor complex (Drosha and its cofactor) to form a stem-loop structure miRNA precursor (pre-miRNA; Yang and Mattes, 2008). For human, the microprocessor complex is consisted of Drosha and DiGeorge syndrome ...
... structure in the pri-miRNA will be cleaved by RNase-III-type enzyme Dicer and nuclear microprocessor complex (Drosha and its cofactor) to form a stem-loop structure miRNA precursor (pre-miRNA; Yang and Mattes, 2008). For human, the microprocessor complex is consisted of Drosha and DiGeorge syndrome ...
Assaying … promoter activity
... • Results and Discussion – The examples on the following slides were all excerpted from real papers – These illustrate common problems that students encounter when drafting their Results and Discussion sections – When reviewing each of these examples, ask yourself whether your own paper could provok ...
... • Results and Discussion – The examples on the following slides were all excerpted from real papers – These illustrate common problems that students encounter when drafting their Results and Discussion sections – When reviewing each of these examples, ask yourself whether your own paper could provok ...
General remarks: A biodesign is a man made, mostly computer
... General Business Terms (GBT) about Molecular Biodesigns, Gene Syntheses and Related Additional Services like Highest Quality Vectors or Expression of Gene Products. 1. General Business Terms This GBTs are regulating the contractual relations and agreements between the molecular biodesigns made execu ...
... General Business Terms (GBT) about Molecular Biodesigns, Gene Syntheses and Related Additional Services like Highest Quality Vectors or Expression of Gene Products. 1. General Business Terms This GBTs are regulating the contractual relations and agreements between the molecular biodesigns made execu ...
Genome-wide analysis of the distribution of AP2/ERF transcription
... Background: Cabbage (Brassica oleracea) is one of the most important leaf vegetables grown worldwide. The entire cabbage genome sequence and more than fifty thousand proteins have been obtained to date. However a high degree of sequence similarity and conserved genome structure remain between cabbag ...
... Background: Cabbage (Brassica oleracea) is one of the most important leaf vegetables grown worldwide. The entire cabbage genome sequence and more than fifty thousand proteins have been obtained to date. However a high degree of sequence similarity and conserved genome structure remain between cabbag ...
Journal of Bacteriology
... become more widespread (2, 14, 30, 38). Under the limited therapeutic options available to treat multidrug-resistant gram-negative bacteria such as Pseudomonas aeruginosa, Acinetobacter baumannii, and Klebsiella pneumoniae, polymyxins are sometimes the only available active antibiotics and have now ...
... become more widespread (2, 14, 30, 38). Under the limited therapeutic options available to treat multidrug-resistant gram-negative bacteria such as Pseudomonas aeruginosa, Acinetobacter baumannii, and Klebsiella pneumoniae, polymyxins are sometimes the only available active antibiotics and have now ...