Supplementary Information (doc 104K)
... are driving the association are more likely to be causal candidates should they converge on a particular mechanism.5,11,12 ...
... are driving the association are more likely to be causal candidates should they converge on a particular mechanism.5,11,12 ...
Cloning, DNA nucleotide sequence and distribution
... mAb, designated 69/25, bound specifically to a fimbrialike structure, designated SEFA, but not to type 1 fimbriae, which were also present on the cell surface of S. enteritidis. Analysis o f the fimbrial antigen indicated a fimbrial subunit of M , 14300 (Thorns et al., 1990). Three discrete fimbrial ...
... mAb, designated 69/25, bound specifically to a fimbrialike structure, designated SEFA, but not to type 1 fimbriae, which were also present on the cell surface of S. enteritidis. Analysis o f the fimbrial antigen indicated a fimbrial subunit of M , 14300 (Thorns et al., 1990). Three discrete fimbrial ...
Finding Regulatory Motifs
... • A microarray experiment showed that when gene X is knocked out, 20 other genes are not expressed. • Motivating Question: How can one gene have such drastic effects? DNA Microarray ...
... • A microarray experiment showed that when gene X is knocked out, 20 other genes are not expressed. • Motivating Question: How can one gene have such drastic effects? DNA Microarray ...
Find information about the protein product of a gene
... Find Category 2 and open the tab-delimited list to access a listing of 4000 short-listed A. thaliana genes of unknown function available for tagging. Study the information for the genes and select one for further analysis. Record the information provided for this locus: o ___________________________ ...
... Find Category 2 and open the tab-delimited list to access a listing of 4000 short-listed A. thaliana genes of unknown function available for tagging. Study the information for the genes and select one for further analysis. Record the information provided for this locus: o ___________________________ ...
The life cycle of fungi - E
... number of means including determinations of base composition, nucleotide sequence, or DNA hybridization rates (c) Typically these means include very powerful ways by which organisms may be classified, either in terms of distinctions between organisms (i.e., the organisms may be classified as represe ...
... number of means including determinations of base composition, nucleotide sequence, or DNA hybridization rates (c) Typically these means include very powerful ways by which organisms may be classified, either in terms of distinctions between organisms (i.e., the organisms may be classified as represe ...
Geneious Sequence Classifier User Manual
... sequence will be classified to the taxonomic level that these database sequences have in common. E.g. for the example dataset given above with ”classify using taxonomy” checked, if this value is set on 0.2%, and the query sequence has 99.3% identity with Acmopyle pancheri and 99.2% identity with Acm ...
... sequence will be classified to the taxonomic level that these database sequences have in common. E.g. for the example dataset given above with ”classify using taxonomy” checked, if this value is set on 0.2%, and the query sequence has 99.3% identity with Acmopyle pancheri and 99.2% identity with Acm ...
Progresses on the knowledge about the ecological function and
... conventional or phase contrast optical microscopy are the most frequent method employed to identify protist species although precise identification is difficult to achieve in this way. The inaccurate assignment of species to other similar morphotypes following previous observations or reports in dep ...
... conventional or phase contrast optical microscopy are the most frequent method employed to identify protist species although precise identification is difficult to achieve in this way. The inaccurate assignment of species to other similar morphotypes following previous observations or reports in dep ...
v2 PEBiosystems News 2 01/00
... AmpliTaq Gold, GeneAmp and TaqMan are registered trademarks of Roche Molecular System, Inc. ICAT is a trademark of the University of Washington, exclusively licensed to Applied Biosystems Group of Applera Corporation. Paracel is a registered trademark of Paracel Inc. The ABI PRISM 3100 and the 3100- ...
... AmpliTaq Gold, GeneAmp and TaqMan are registered trademarks of Roche Molecular System, Inc. ICAT is a trademark of the University of Washington, exclusively licensed to Applied Biosystems Group of Applera Corporation. Paracel is a registered trademark of Paracel Inc. The ABI PRISM 3100 and the 3100- ...
genetics: the code broken
... ‘regulatory’ genes, which produce proteins that can control the transcription stage and other aspects of protein synthesis. Characteristics can be determined by more than one pair of alleles (‘multiple alleles’) within a gene pair; examples of this include the inheritance of blood groups and Rh anti ...
... ‘regulatory’ genes, which produce proteins that can control the transcription stage and other aspects of protein synthesis. Characteristics can be determined by more than one pair of alleles (‘multiple alleles’) within a gene pair; examples of this include the inheritance of blood groups and Rh anti ...
Find expression data for a tagged gene
... thaliana genes of unknown function available for tagging. Study the information for the genes and select one for further analysis: o _________________________________________________________________ o _________________________________________________________________ o _______________________________ ...
... thaliana genes of unknown function available for tagging. Study the information for the genes and select one for further analysis: o _________________________________________________________________ o _________________________________________________________________ o _______________________________ ...
Document
... Biological Concept Spaces (2005) Compute concept spaces for All of Biology BioSpace across entire biomedical literature 50M abstracts across 50K repositories Use Gene Ontology to partition literature into biological communities for functional analysis GO same scale as MeSH but adequate coverage? GO ...
... Biological Concept Spaces (2005) Compute concept spaces for All of Biology BioSpace across entire biomedical literature 50M abstracts across 50K repositories Use Gene Ontology to partition literature into biological communities for functional analysis GO same scale as MeSH but adequate coverage? GO ...
Molecular characterization of dioxygenases from polycyclic aromatic
... and two amino acid throughout the uniform protein size of 169 amino acids. The Rieske center iron^sulfur binding site [4], CXHRGX8 GNX5 CXZHG, was found to be conserved in all deduced NidA proteins. Also, two histidine residues and one aspartate residue, which according to Parales et al. [6] bind th ...
... and two amino acid throughout the uniform protein size of 169 amino acids. The Rieske center iron^sulfur binding site [4], CXHRGX8 GNX5 CXZHG, was found to be conserved in all deduced NidA proteins. Also, two histidine residues and one aspartate residue, which according to Parales et al. [6] bind th ...
"Frontmatter". In: Plant Genomics and Proteomics
... (genome sizes) that occur in the plant kingdom, even between closely related species. Therefore, it is harder to define the nature of a typical plant genome because the contribution of additional DNA may have phenotypic effects independent of the actual sequences of DNA present, for example, the rol ...
... (genome sizes) that occur in the plant kingdom, even between closely related species. Therefore, it is harder to define the nature of a typical plant genome because the contribution of additional DNA may have phenotypic effects independent of the actual sequences of DNA present, for example, the rol ...
Molecular Mechanisms of Plant and Microbe Coexistence
... containing compounds of root exudates. A partial answer has been obtained by using reporter bacteria responding specifically to individual amino acids. The induction of a lysine-responsive P. putida reporter was demonstrated in rhizosphere of corn, but not in the bulk soil (Espinosa-Urgel and Ramos ...
... containing compounds of root exudates. A partial answer has been obtained by using reporter bacteria responding specifically to individual amino acids. The induction of a lysine-responsive P. putida reporter was demonstrated in rhizosphere of corn, but not in the bulk soil (Espinosa-Urgel and Ramos ...
Instructions for ICML-98 Authors
... understanding gene regulation and interactions, expression levels for thousands of genes simultaneously. The ability to successfully microarrays have very important applications in pharmaceutical and clinical research. By comparing analyze the huge amounts of genomic data is of gene expression in no ...
... understanding gene regulation and interactions, expression levels for thousands of genes simultaneously. The ability to successfully microarrays have very important applications in pharmaceutical and clinical research. By comparing analyze the huge amounts of genomic data is of gene expression in no ...
Acquired Copy Number Alterations in Adult Acute Myeloid Leukemia
... Figure 1: Copy number and UPD heatmap for 86 AML genomes. The results of copy number and UPD (copy-neutral LOH) analysis of 86 paired tumor and normal DNA research samples assayed on the Affymetrix Genome-Wide SNP 6.0 arrays are shown. Each of the 86 genomes is represented by two columns, copy numbe ...
... Figure 1: Copy number and UPD heatmap for 86 AML genomes. The results of copy number and UPD (copy-neutral LOH) analysis of 86 paired tumor and normal DNA research samples assayed on the Affymetrix Genome-Wide SNP 6.0 arrays are shown. Each of the 86 genomes is represented by two columns, copy numbe ...
BLAST Exercise: Detecting and Interpreting Genetic Homology
... expect the same result if it had come from a primate? Translated Query vs. Protein Database (blastx): The Gene Hunter Following the initial annotation of repetitive elements found within our sequence (dmel_seq2.fasta), we would like to see if any part of our sequence matches any known genes. We will ...
... expect the same result if it had come from a primate? Translated Query vs. Protein Database (blastx): The Gene Hunter Following the initial annotation of repetitive elements found within our sequence (dmel_seq2.fasta), we would like to see if any part of our sequence matches any known genes. We will ...
Brand, Veronica - Degenerate Primer Design using Computational Tools
... such: “Given a training set of known genes, design a pair of primers, one for the 5’ side and another for the 3’ side, so that the primers would amplify many of the genes and would have degeneracy that does not exceed a predefined limit” (2005). A degenerate primer in this case is a primer where mul ...
... such: “Given a training set of known genes, design a pair of primers, one for the 5’ side and another for the 3’ side, so that the primers would amplify many of the genes and would have degeneracy that does not exceed a predefined limit” (2005). A degenerate primer in this case is a primer where mul ...
Reconstruction of a 450-My-old ancestral vertebrate protokaryotype
... consists of the large TNI 2 and 3 chromosomes, which are included in chromosomes TET 2, 3, 8 and 11 (Table 2), implying that TEL 1 is a large chromosome. By contrast, protochromosome TEL 12 consists of segments of TNI 6 and 15 and corresponds to the small chromosome OLA 20. Similar reconstructions o ...
... consists of the large TNI 2 and 3 chromosomes, which are included in chromosomes TET 2, 3, 8 and 11 (Table 2), implying that TEL 1 is a large chromosome. By contrast, protochromosome TEL 12 consists of segments of TNI 6 and 15 and corresponds to the small chromosome OLA 20. Similar reconstructions o ...
Evolution of Immunoglobulin Kappa Chain Variable Region
... overall sequence divergence between the two sets of duplicate genes (;1%), Schäble and Zachau (1993) suggested that the duplication occurred about 1 MYA. The copy of the Vk region adjacent to Jk genes is called the p (proximal) contig, whereas the other copy of this region is called the d (distal) ...
... overall sequence divergence between the two sets of duplicate genes (;1%), Schäble and Zachau (1993) suggested that the duplication occurred about 1 MYA. The copy of the Vk region adjacent to Jk genes is called the p (proximal) contig, whereas the other copy of this region is called the d (distal) ...
The Spectrum and Frequency of Self
... with a mutant phenotype from several Ac donor lines and found only 24 such Ac inactivation events. The system that we report on here is highly efficient for isolating these more rare events, which most likely arise from DSB repair by an error-prone DNA synthesis pathway and from aberrant transpositio ...
... with a mutant phenotype from several Ac donor lines and found only 24 such Ac inactivation events. The system that we report on here is highly efficient for isolating these more rare events, which most likely arise from DSB repair by an error-prone DNA synthesis pathway and from aberrant transpositio ...
Bacteria Transformation
... human insulin. The technology which made this approach possible was the development of recombinant DNA techniques. In simple terms, the human gene which codes for the insulin protein was cloned (copied) and then put inside of bacteria. A number of tricks were performed on this gene to make the bacte ...
... human insulin. The technology which made this approach possible was the development of recombinant DNA techniques. In simple terms, the human gene which codes for the insulin protein was cloned (copied) and then put inside of bacteria. A number of tricks were performed on this gene to make the bacte ...
Association Studies of Vascular Phenotypes
... DNA into RNA, affect the processing or stability of the RNA or the protein, or change the structure of the protein. A second option is that the genotyped marker is not itself functional, but is in linkage disequilibrium with other polymorphisms that are functional. Linkage disequilibrium, explained ...
... DNA into RNA, affect the processing or stability of the RNA or the protein, or change the structure of the protein. A second option is that the genotyped marker is not itself functional, but is in linkage disequilibrium with other polymorphisms that are functional. Linkage disequilibrium, explained ...
Supporting Information Legends Supporting Figure 1. Amino acid
... experiments. (A) Immunoblotting of the gluc protein in the indicated strains. Gluc(9–12 mm)-2×ago3-1(AGO3)-2 was generated by a genetic cross between Gluc(9–12 mm)-2 and ago3-1(AGO3)-2. This strain expresses the gluc(9–12 mm) transgene and tagged-AGO3 in the ago3-1 background. (B) Northern blotting ...
... experiments. (A) Immunoblotting of the gluc protein in the indicated strains. Gluc(9–12 mm)-2×ago3-1(AGO3)-2 was generated by a genetic cross between Gluc(9–12 mm)-2 and ago3-1(AGO3)-2. This strain expresses the gluc(9–12 mm) transgene and tagged-AGO3 in the ago3-1 background. (B) Northern blotting ...
CGMD: An integrated database of Cancer Genes and Markers
... ‘pathways,’ ‘gene description,’ ‘protein description,’ (Figure 3F&G) by clicking the ‘pathways’ it representing by displaying the list of cancer markers and its pathways and the number of tumor suppressor genes involvement and their detailed descriptions with respective types of cancers (Figure 3H). ...
... ‘pathways,’ ‘gene description,’ ‘protein description,’ (Figure 3F&G) by clicking the ‘pathways’ it representing by displaying the list of cancer markers and its pathways and the number of tumor suppressor genes involvement and their detailed descriptions with respective types of cancers (Figure 3H). ...
Metagenomics
Metagenomics is the study of genetic material recovered directly from environmental samples. The broad field may also be referred to as environmental genomics, ecogenomics or community genomics. While traditional microbiology and microbial genome sequencing and genomics rely upon cultivated clonal cultures, early environmental gene sequencing cloned specific genes (often the 16S rRNA gene) to produce a profile of diversity in a natural sample. Such work revealed that the vast majority of microbial biodiversity had been missed by cultivation-based methods. Recent studies use either ""shotgun"" or PCR directed sequencing to get largely unbiased samples of all genes from all the members of the sampled communities. Because of its ability to reveal the previously hidden diversity of microscopic life, metagenomics offers a powerful lens for viewing the microbial world that has the potential to revolutionize understanding of the entire living world. As the price of DNA sequencing continues to fall, metagenomics now allows microbial ecology to be investigated at a much greater scale and detail than before.