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Brevifollis gellanilyticus gen. nov., sp. nov., a gellan-gum
Brevifollis gellanilyticus gen. nov., sp. nov., a gellan-gum

... the number of cultured strains is still limited. Improved PCR techniques and metagenomic analyses are now revealing the ubiquitous distribution and even, in some cases, the dominance of members of the phylum Verrucomicrobia in the environment (Bergmann et al., 2011; Kielak et al., 2010). However, th ...
VanBUG_quackenbush
VanBUG_quackenbush

... Conceptual Schema: MAD Clone Slide ...
Investigation 1: Identify the Transcriptional Unit
Investigation 1: Identify the Transcriptional Unit

... RNA-Seq data hence becomes our first line of evidence to try to determine the location of the TSS. In other words the information gathered from RNA-Seq will be used to support the choice of the TSS. To learn more about RNA-Seq, watch the RNA-Seq and TopHat video: https://youtu.be/qepVXEsfLMM Q4.Exam ...
Remarkably Little Variation in Proteins Encoded
Remarkably Little Variation in Proteins Encoded

... may be due to higher variance in reproductive success among men than among women,8 which would further reduce the effective population size of Y chromosomes. The results reported here shed new light on an important question: how representative or typical is the sequenced human Y chromosome? Previou ...
Evolution of the defensin-like gene family in grass genomes
Evolution of the defensin-like gene family in grass genomes

... ZmDf-10/11 and ZmDf-14/15, respectively. To determine whether the flanking genes of DEFL have undergone large-scale duplication events during the evolution of DEFL gene families, we compared the flanking genes of any two DEFL genes. If three or more flanking members had a best nonself match according t ...
Biology TEST: Chapter 18 Classification (Form MRK:2008)
Biology TEST: Chapter 18 Classification (Form MRK:2008)

... The procedure of grouping organisms based on their evolutionary history is called a. traditional classification. b. binomial nomenclature. c. derived characters. d. evolutionary classification. In an evolutionary classification scheme, species within one genus should a. be more similar to each other ...
Protein World
Protein World

... • This was measured by using the KEGG Pathway map (release 25) • The best, however not completely convincing, result was found using PCP and not ME: ...
Chapter 18
Chapter 18

... cytoplasm and are often present in small amounts, so a cDNA library is a “snapshot” of the transcription pattern of the cell. cDNA libraries are used to compare gene expression in different tissues at different stages of development. ...
Citrate Synthase Gene Comparison, a New Tool for Phylogenetic
Citrate Synthase Gene Comparison, a New Tool for Phylogenetic

... for the members of the genus Rickettsia (levels of similarity between two species, more than 97.2%); thus, reliable phylogenetic analysis based on a comparison of the 16s rRNA gene sequences has been difficult, especially for the members of the SFG. The importance of comparing results obtained for m ...
DNA SEQUENCING (using a Li
DNA SEQUENCING (using a Li

... fragment, then the run time will be short (1-2 h). If the fragment is long, and the desired sequence is closer to the 3' end, then the run time will be longer (2-5 h). The other method of sequencing DNA (Sanger 1977) uses a polymerization reaction (using DNA polymerase) in conjunction with mixtures ...
Low diversity in the major histocompatibility complex class II DRB1
Low diversity in the major histocompatibility complex class II DRB1

... PCR-RFLP and sequence analyses of the Capy-DRB1 gene in three subspecies of the SI allowed us to detect six distinct alleles (Genbank accession numbers AF461692AF46196 and AY351788). One of these DRB1 alleles (Capy-DRB1.6) was found only in CPV whereas two other alleles (Capy-DRB1.4 and Capy-DRB1.5) ...
Pleurochrysome: A Web Database of
Pleurochrysome: A Web Database of

... UNIGENEs) assembled from expressed sequence tag sequences of P. haptonemofera as core information. The UNIGENEs were annotated with gene sequences sharing significant homology, conserved domains, Gene Ontology, KEGG Orthology, predicted subcellular localization, open reading frames and orthologous r ...
Chap 8 Recombinant DNA technology Fall 2012
Chap 8 Recombinant DNA technology Fall 2012

... • Gene Libraries – A collection of bacterial or phage clones – Each clone in library often contains one gene of an organism’s genome ...
Name that Gene
Name that Gene

... This will take you to a page with lots of information about your gene and what it does. Some of the information may be difficult to follow. You can always search on the internet to find out more! ***So that is your basic tour of the NCBI. There is lots of other information about genes and DNA posted ...
module 2: transcription part i
module 2: transcription part i

... RNA-Seq data hence becomes our first line of evidence to try to determine the location of the TSS. In other words the information gathered from RNA-Seq will be used to support the choice of the TSS. To learn more about RNA-Seq, watch the RNA-Seq and TopHat video: https://youtu.be/qepVXEsfLMM Q4.Exam ...
DNA, Technology, and Florida Strawberries 1 - EDIS
DNA, Technology, and Florida Strawberries 1 - EDIS

... the EDIS website at http://edis.ifas.ufl.edu. 2. Seonghee Lee, assistant professor, Department of Horticultural Sciences, UF/IFAS Gulf Coast Research and Education Center; Young-Hee Noh, visiting scientist, Horticultural Sciences Department, UF/IFAS Gulf Coast Research and Education Center; Sujeet ...
AP Biology: Evolution
AP Biology: Evolution

... Where were restriction enzymes first discovered? (What type of organism?) Why did this type of organism develop these enzymes? 1. In the RE lab we used lambda DNA which is a “phage”. What does that mean? Sketch a picture of how it operates. What is lambda DNA commonly used for? 2. What temperature d ...
Allele replacement: an application that permits rapid manipulation of
Allele replacement: an application that permits rapid manipulation of

... present in bacteria that could give a false PCR result (see Figure 5b). Once detected, BAC DNA can be harvested from bacteria and transfected into mammalian cells and virus recovered 2 days later. Taken together, this procedure allows generation and isolation of mutant viruses within 7 days. Moreove ...
BIOL 1010
BIOL 1010

... into a bacterium, using a plasmid as a vector (see next paragraph) – the bacterium could then produce the human form of insulin. A plasmid is a circlet of DNA found in a bacterium. Plasmids are unique to bacteria and are a means by which bacteria can actually exchange genetic material. Scientists ha ...
Lecture PPT - Carol Lee Lab
Lecture PPT - Carol Lee Lab

... 2. You are a medical researcher working on HIV. A novel strain has appeared in Madison, Wisconsin. To determine which drugs would be most effective in treating this new strain (because different strains are resistant to different drugs), you need to determine its recent evolutionary history. You de ...
Full Text  - Science and Education Publishing
Full Text - Science and Education Publishing

... substitution of aspartic acid in TEM-1(gb: AFI61435.1) to Threonine at position 262. In Silico tools was used for mutation analysis and prediction of secondary and tertiary structure of wild and mutant type genes. We conclude that our mutant gene is completely different from the wild types TEM-1 gen ...
STAT115 STAT225 BIST512 BIO298 - Intro to Computational Biology
STAT115 STAT225 BIST512 BIO298 - Intro to Computational Biology

... STAT115 STAT225 BIST512 BIO298 - Intro to Computational Biology ...
Lab exam 1 V DONE
Lab exam 1 V DONE

... same location, but four of them map to chromosome 4 and the remaining six map to chromosome 6. Other scientists get the same data as well (for the same species) and even confirm these loci encode for mRNA. Select the answer that explains these results. Select either D or E if you think there are two ...
Regions of XY homology in the pig X pseudoautosomal region
Regions of XY homology in the pig X pseudoautosomal region

... further complicates attempts to sequence this chromosome, a problem common to mammalian Y chromosomes (e.g. mouse; [11-15]). Some clues as to the structure of the pig Y have come from the search for male specific DNA sequences. These yielded short repetitive sequences [16-19], which appear male-spec ...
Microarray Data analysis - Functional Glycomics Gateway
Microarray Data analysis - Functional Glycomics Gateway

... DAVID, or Database for Annotation, Visualization and Integrated Discovery, provides integrated solutions for the annotation and analysis of genomescale datasets derived from high-throughput technologies such as microarray and proteomic platforms. This tutorial will demonstrate how to use DAVID 1.0. ...
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Metagenomics



Metagenomics is the study of genetic material recovered directly from environmental samples. The broad field may also be referred to as environmental genomics, ecogenomics or community genomics. While traditional microbiology and microbial genome sequencing and genomics rely upon cultivated clonal cultures, early environmental gene sequencing cloned specific genes (often the 16S rRNA gene) to produce a profile of diversity in a natural sample. Such work revealed that the vast majority of microbial biodiversity had been missed by cultivation-based methods. Recent studies use either ""shotgun"" or PCR directed sequencing to get largely unbiased samples of all genes from all the members of the sampled communities. Because of its ability to reveal the previously hidden diversity of microscopic life, metagenomics offers a powerful lens for viewing the microbial world that has the potential to revolutionize understanding of the entire living world. As the price of DNA sequencing continues to fall, metagenomics now allows microbial ecology to be investigated at a much greater scale and detail than before.
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