Methods S1
... based on previous studies (Paolacci et al., 2009; Gimenez et al., 2011) and data on Genevestigator (Hruz et al., 2008) by searching for most stably expressed genes in leaves of wheat seedlings. Primers were designed for the reference genes RLIL and TA.6863 using Primer-blast (Ye et al., 2012) to tar ...
... based on previous studies (Paolacci et al., 2009; Gimenez et al., 2011) and data on Genevestigator (Hruz et al., 2008) by searching for most stably expressed genes in leaves of wheat seedlings. Primers were designed for the reference genes RLIL and TA.6863 using Primer-blast (Ye et al., 2012) to tar ...
Examination IV Key
... the DNA migrates to the positive electrode with the longest DNA moving the most rapidly the DNA migrates to the positive electrode with the shortest DNA moving the most rapidly the DNA migrates to the negative electrode with the longest DNA moving the most rapidly the DNA migrates to the negative el ...
... the DNA migrates to the positive electrode with the longest DNA moving the most rapidly the DNA migrates to the positive electrode with the shortest DNA moving the most rapidly the DNA migrates to the negative electrode with the longest DNA moving the most rapidly the DNA migrates to the negative el ...
Genoma
... translating it into tangible benefits for society: technology advancement accessible throughout Europe at an affordable price • Local presence and Support with close proximity to clients and medical community • Very high throughput platform (over 100’000 samples/year) ...
... translating it into tangible benefits for society: technology advancement accessible throughout Europe at an affordable price • Local presence and Support with close proximity to clients and medical community • Very high throughput platform (over 100’000 samples/year) ...
Gene conversion analysis of the mouse Pilr locus
... Gene conversion analysis of the mouse Pilr locus. Gene conversion has played a role in shaping the mouse Pilr locus and is detected by a variety of phylogenetic and statistical methods. Phylogenetic analysis of a conserved 3 kb region (the promoter through to intron 3) suggests that Pilrb1 and Pilrb ...
... Gene conversion analysis of the mouse Pilr locus. Gene conversion has played a role in shaping the mouse Pilr locus and is detected by a variety of phylogenetic and statistical methods. Phylogenetic analysis of a conserved 3 kb region (the promoter through to intron 3) suggests that Pilrb1 and Pilrb ...
Preparation of SCRATCHY Hybrid Protein Libraries
... The experimental implementation of SCRATCHY consists of two successive steps, an initial creation of an ITCHY library, followed by a homologous recombination procedure such as DNA shuffling (see Fig. 1). In the process, the ITCHY library serves as an artificial family of hybrid sequences that, upon ...
... The experimental implementation of SCRATCHY consists of two successive steps, an initial creation of an ITCHY library, followed by a homologous recombination procedure such as DNA shuffling (see Fig. 1). In the process, the ITCHY library serves as an artificial family of hybrid sequences that, upon ...
Why does the giraffe have such a long neck? Analysis zeroes in on
... Most of the genes Cavener and Agaba found help determine how the bodies of other animals form. This supports the idea that tweaking these genes could change an animal's physical qualities. For example, a small change in genes could make a donkey-like creature incredibly tall. The scientists found so ...
... Most of the genes Cavener and Agaba found help determine how the bodies of other animals form. This supports the idea that tweaking these genes could change an animal's physical qualities. For example, a small change in genes could make a donkey-like creature incredibly tall. The scientists found so ...
PDF
... ABSTRACT: β-Lactoglobulin (β-LG) is the dominant non-casein whey protein found in milk of bovine and of most ruminants. The amino acid sequence of β-LG along with its 3-dimensional structure illustrates linkage with the lipocalin superfamily. Preliminary studies in goats indicated that milk yield ca ...
... ABSTRACT: β-Lactoglobulin (β-LG) is the dominant non-casein whey protein found in milk of bovine and of most ruminants. The amino acid sequence of β-LG along with its 3-dimensional structure illustrates linkage with the lipocalin superfamily. Preliminary studies in goats indicated that milk yield ca ...
Rare genomic changes and mitochondrial sequences
... Sequences from 34 additional complete arthropod mitochondrial genomes that span the diversity of arthropods were selected for phylogenetic analyses, with the onychophoran Epiperipatus used as an outgroup (see Table 1 for a list of taxa). We purposefully excluded candidate mitogenomic data for severa ...
... Sequences from 34 additional complete arthropod mitochondrial genomes that span the diversity of arthropods were selected for phylogenetic analyses, with the onychophoran Epiperipatus used as an outgroup (see Table 1 for a list of taxa). We purposefully excluded candidate mitogenomic data for severa ...
Rearrangements of the Blood Group RhD Gene
... and our unpublished data) we deduced that the 5' and 3' boundaries of the deletion in the transcription product corresponded to the exon 3lintron 3 and intron6/exon l transitions, respectively. This result, together with the Southern analysis described above, showed that the genomic region encompass ...
... and our unpublished data) we deduced that the 5' and 3' boundaries of the deletion in the transcription product corresponded to the exon 3lintron 3 and intron6/exon l transitions, respectively. This result, together with the Southern analysis described above, showed that the genomic region encompass ...
FAQ 2015 HGMD - Frequently Asked Questions
... associated/functional polymorphisms reported in the literature. HGMD may also include additional reports for certain mutations if these reports serve to enhance the original entry (e.g. functional studies). To be included, there must be a convincing association of the polymorphism with the phenotype ...
... associated/functional polymorphisms reported in the literature. HGMD may also include additional reports for certain mutations if these reports serve to enhance the original entry (e.g. functional studies). To be included, there must be a convincing association of the polymorphism with the phenotype ...
Organisation of the human genome and our tools for
... gene is 2000 base pairs. The size of human genes varies from hundreds of bases to several megabases especially due to the large intronic sequences. The human Dystrophin gene is 2, 4 Mb (2 400 000 bases) including 79 exons. The non-coding part of the genome (98%) is not ‘junk’ DNA. It contains numero ...
... gene is 2000 base pairs. The size of human genes varies from hundreds of bases to several megabases especially due to the large intronic sequences. The human Dystrophin gene is 2, 4 Mb (2 400 000 bases) including 79 exons. The non-coding part of the genome (98%) is not ‘junk’ DNA. It contains numero ...
Lecture Presentation to accompany Principles of Life
... Share a Common Ancestor • 19.2 Prokaryote Diversity Reflects the Ancient Origins of Life ...
... Share a Common Ancestor • 19.2 Prokaryote Diversity Reflects the Ancient Origins of Life ...
DEVELOPMENT AND CHARACTERIZATION OF SULFIDE-OXIDIZING BIOFILMS UNIVERSITAT AUTÒNOMA DE BARCELONA Facultat de Ciències
... Another group of compounds which contribute massively to pollution are reduced sulfur compounds. They constitute a group of organic (carbon disulfide, dimethyl sulfide, methane thiol, thiophenes among others) and inorganic (hydrogen sulfide, thiosulfate, polysulfides, polythionates) chemical compoun ...
... Another group of compounds which contribute massively to pollution are reduced sulfur compounds. They constitute a group of organic (carbon disulfide, dimethyl sulfide, methane thiol, thiophenes among others) and inorganic (hydrogen sulfide, thiosulfate, polysulfides, polythionates) chemical compoun ...
Functional Genomics and the Path from Genetic
... Functional Genomics and the Path from Genetic Variation to Clinical Translation 2nd Annual Cross-Disciplinary Symposium on the State of Science, Technology, and Capacity at Washington University in St. Louis Sponsored by the Intellectual and Developmental Disabilities Research Center and St. Louis C ...
... Functional Genomics and the Path from Genetic Variation to Clinical Translation 2nd Annual Cross-Disciplinary Symposium on the State of Science, Technology, and Capacity at Washington University in St. Louis Sponsored by the Intellectual and Developmental Disabilities Research Center and St. Louis C ...
Baseline surveys
... Habitat re-created with mist sprinkler system Population rebounded but then hit by chytrid fungus Captive breeding started with 499 individuals Reintroduced 1700 to the wild in 2012 ...
... Habitat re-created with mist sprinkler system Population rebounded but then hit by chytrid fungus Captive breeding started with 499 individuals Reintroduced 1700 to the wild in 2012 ...
PCR Applications
... 11) Obtain a PCR tube (much smaller than microfuge tube) that contains “Ready to go ™ bead and add 22.5 L of PTC primer/loading dye mix to this tube. Wait for the bead to dissolve, at least 1 minute.. 12) To the tube from step11, add 2.5 L of your check cell supernatant (step 10). Keep on ice unti ...
... 11) Obtain a PCR tube (much smaller than microfuge tube) that contains “Ready to go ™ bead and add 22.5 L of PTC primer/loading dye mix to this tube. Wait for the bead to dissolve, at least 1 minute.. 12) To the tube from step11, add 2.5 L of your check cell supernatant (step 10). Keep on ice unti ...
Principal Component Analysis for Clustering Gene
... criterion. “Cluster” refers to clusters obtained by a clustering algorithm. There are two real data sets and three synthetic data sets in this study. ...
... criterion. “Cluster” refers to clusters obtained by a clustering algorithm. There are two real data sets and three synthetic data sets in this study. ...
BAC vectors (Bacterial Artificial Chromosome)
... not essential for viral growth are removed from the viral DNA and replaced with the DNA to be cloned. Up to ~25 kb of foreign DNA can be inserted into the λ genome, resulting in a recombinant DNA that can be packaged in vitro to form virions capable of replicating and forming plagues on E. coli host ...
... not essential for viral growth are removed from the viral DNA and replaced with the DNA to be cloned. Up to ~25 kb of foreign DNA can be inserted into the λ genome, resulting in a recombinant DNA that can be packaged in vitro to form virions capable of replicating and forming plagues on E. coli host ...
QPCR Analysis of Bacterial DNA Extractions
... mystery, it has been associated with three characteristics: a rise in pH (> 4.5), an overgrowth of pathogenic anaerobic bacteria, and a reduction in lactobacilli, the bacteria present in the healthy vagina. Lactobacilli produce lactic acid and hydrogen peroxide, maintaining the low pH of the vagina ...
... mystery, it has been associated with three characteristics: a rise in pH (> 4.5), an overgrowth of pathogenic anaerobic bacteria, and a reduction in lactobacilli, the bacteria present in the healthy vagina. Lactobacilli produce lactic acid and hydrogen peroxide, maintaining the low pH of the vagina ...
Deficiency γ-α Genetic Basis of Human Complement C8
... two-step procedure with PCR-SSCP analysis as a first step followed by a second step of sequencing the aberrant bands. In the first step, all 11 exons of the C8a and the 7 exons of the C8g gene were amplified by PCR, and the resulting DNA fragments were analyzed by SSCP. This approach enabled us to d ...
... two-step procedure with PCR-SSCP analysis as a first step followed by a second step of sequencing the aberrant bands. In the first step, all 11 exons of the C8a and the 7 exons of the C8g gene were amplified by PCR, and the resulting DNA fragments were analyzed by SSCP. This approach enabled us to d ...
DNA sequence annotation
... 2. In this project you will work with real data. 3. You will get the DNA sequence by e-mail. The sequence is saved in the text file and it is one long string of characters without spaces or new lines. 4. Please, read the full project description, before you start to write your programs. You don’t ne ...
... 2. In this project you will work with real data. 3. You will get the DNA sequence by e-mail. The sequence is saved in the text file and it is one long string of characters without spaces or new lines. 4. Please, read the full project description, before you start to write your programs. You don’t ne ...
Guidelines for interpretation of 16S rRNA gene sequence
... bacterial species based on similarity search results, as no threshold values are available as for DNA–DNA hybridization (Petti, 2007). As different bacterial species are likely to evolve at different rates, there has been no agreement on a universal cut-off for bacterial species delineation. In 1994 ...
... bacterial species based on similarity search results, as no threshold values are available as for DNA–DNA hybridization (Petti, 2007). As different bacterial species are likely to evolve at different rates, there has been no agreement on a universal cut-off for bacterial species delineation. In 1994 ...
Penn rDNA Registration Forms
... current NIH guidelines for the Biosafety Level you have indicated above, unless modified by the IBC; that you accept responsibility for the safe conduct of the experiments conducted at this Biosafety Level; and that you have informed all associated personnel of the conditions required for this work. ...
... current NIH guidelines for the Biosafety Level you have indicated above, unless modified by the IBC; that you accept responsibility for the safe conduct of the experiments conducted at this Biosafety Level; and that you have informed all associated personnel of the conditions required for this work. ...
Feng Zhang, Ph.D.
... successfully harnessed a second system, called CRISPR-Cpf1, which has the potential for even simpler and more precise genome engineering. Zhang and the Broad Institute are committed to making CRISPR technology widely available to the entire scientific community. The Zhang lab has trained thousands o ...
... successfully harnessed a second system, called CRISPR-Cpf1, which has the potential for even simpler and more precise genome engineering. Zhang and the Broad Institute are committed to making CRISPR technology widely available to the entire scientific community. The Zhang lab has trained thousands o ...
Supplementary Note
... weak positives were recovered, but none proved to contain a relative of SRY. Screening the same libraries with tammar SOX3 yielded many positive clones, but these proved to contain either unrelated sequences, or SOX genes that were present in both sexes. Two platypus SOXB genes (SOX2 and SOX14) were ...
... weak positives were recovered, but none proved to contain a relative of SRY. Screening the same libraries with tammar SOX3 yielded many positive clones, but these proved to contain either unrelated sequences, or SOX genes that were present in both sexes. Two platypus SOXB genes (SOX2 and SOX14) were ...
Metagenomics
Metagenomics is the study of genetic material recovered directly from environmental samples. The broad field may also be referred to as environmental genomics, ecogenomics or community genomics. While traditional microbiology and microbial genome sequencing and genomics rely upon cultivated clonal cultures, early environmental gene sequencing cloned specific genes (often the 16S rRNA gene) to produce a profile of diversity in a natural sample. Such work revealed that the vast majority of microbial biodiversity had been missed by cultivation-based methods. Recent studies use either ""shotgun"" or PCR directed sequencing to get largely unbiased samples of all genes from all the members of the sampled communities. Because of its ability to reveal the previously hidden diversity of microscopic life, metagenomics offers a powerful lens for viewing the microbial world that has the potential to revolutionize understanding of the entire living world. As the price of DNA sequencing continues to fall, metagenomics now allows microbial ecology to be investigated at a much greater scale and detail than before.