Genetic Technology
... Mapping and Sequencing the Human Genome • In 1990, scientists in the United States organized the Human Genome Project (HGP). It is an international effort to completely map and sequence the human genome, the approximately 35 000-40 000 genes on the 46 human chromosomes. ...
... Mapping and Sequencing the Human Genome • In 1990, scientists in the United States organized the Human Genome Project (HGP). It is an international effort to completely map and sequence the human genome, the approximately 35 000-40 000 genes on the 46 human chromosomes. ...
The aquaporin-Z water channel gene of Escherichia co/i
... al, 1995). As revealed by phylogenetic analyses, functional distinctions between AqpZ and GlpF probably resulted from the ancient divergence in a primordial gene (Calamita et al, 1995; Park and Saier, 1996), however, uqpZ and glpF genes are not present in the genome of the archaeon Methunococcus jun ...
... al, 1995). As revealed by phylogenetic analyses, functional distinctions between AqpZ and GlpF probably resulted from the ancient divergence in a primordial gene (Calamita et al, 1995; Park and Saier, 1996), however, uqpZ and glpF genes are not present in the genome of the archaeon Methunococcus jun ...
Anatomy and Physiology BIO 137
... genome as opposed to highly-conserved genes. • PCR can be used to amplify highly variable regions of the human genome. These regions contain runs of short, repeated sequences (known as variable number of tandem repeat (VNTR) sequences) . The number of repeats can vary from 4-40 in different individu ...
... genome as opposed to highly-conserved genes. • PCR can be used to amplify highly variable regions of the human genome. These regions contain runs of short, repeated sequences (known as variable number of tandem repeat (VNTR) sequences) . The number of repeats can vary from 4-40 in different individu ...
Supplemental Material
... Figure S2: A) SDS-Page gels of purified the Rtf1 domains. The molecular sizes of marker proteins are given to the left. B) Western analysis of shifted material. Gel shifts were obtained in duplicate under standard conditions (domains and linkers are given in the panel). One set of gelshifted materia ...
... Figure S2: A) SDS-Page gels of purified the Rtf1 domains. The molecular sizes of marker proteins are given to the left. B) Western analysis of shifted material. Gel shifts were obtained in duplicate under standard conditions (domains and linkers are given in the panel). One set of gelshifted materia ...
10-Evidence for Evolution 4
... the similarities and differences between parents and their children are heard often in conversation. These similar traits are due to the genetic material that children inherit from their parents. As humans we are sometimes fortunate enough to have three or four generations sitting in a room at one t ...
... the similarities and differences between parents and their children are heard often in conversation. These similar traits are due to the genetic material that children inherit from their parents. As humans we are sometimes fortunate enough to have three or four generations sitting in a room at one t ...
#1
... ASE composition is spatially structured in mammalian genomes. From sodium chloride centrifugation experiments, Bernardi et al. (1985) defined three major classes of genomic fragments with low, median, and high GC content, respectively, and called them isochores. This discrete description now appears ...
... ASE composition is spatially structured in mammalian genomes. From sodium chloride centrifugation experiments, Bernardi et al. (1985) defined three major classes of genomic fragments with low, median, and high GC content, respectively, and called them isochores. This discrete description now appears ...
Example Use Case – Transcriptional Profiling
... • Pearson correlation as a database query: - Data matrix: 60,000+ samples x 60,000+ probes ...
... • Pearson correlation as a database query: - Data matrix: 60,000+ samples x 60,000+ probes ...
- Murdoch Research Repository
... B. hyodysenteriae has been shown to contain an unusual prophage-like agent named VSH-1 (virus of Serpulina hyodysenteriae) that is involved in natural gene transfer and recombination within the species (Humphrey et al., 1997 and Matson et al., 2007). This agent is in a state of permanent lysogeny an ...
... B. hyodysenteriae has been shown to contain an unusual prophage-like agent named VSH-1 (virus of Serpulina hyodysenteriae) that is involved in natural gene transfer and recombination within the species (Humphrey et al., 1997 and Matson et al., 2007). This agent is in a state of permanent lysogeny an ...
Bacterial Growth and Metabolism on Surfaces in the Large Intestine
... (5) thus, while we can determine that the ecosystem contains large numbers of phylogenetically and physiologically different bacteria, the relative population sizes and types of non-culturable organisms present in the microbiota are largely unknown. Many host and environmental factors affect the com ...
... (5) thus, while we can determine that the ecosystem contains large numbers of phylogenetically and physiologically different bacteria, the relative population sizes and types of non-culturable organisms present in the microbiota are largely unknown. Many host and environmental factors affect the com ...
Genetic and functional characterization of the gene cluster directing
... colony as described previously (Dubern et al., 2005). Culture supernatants of the selected mutants, obtained after growth overnight in KB medium, were analysed for the presence of surfactant production using the drop-collapsing assay (Jain et al., 1991). To isolate the chromosomal DNA region flankin ...
... colony as described previously (Dubern et al., 2005). Culture supernatants of the selected mutants, obtained after growth overnight in KB medium, were analysed for the presence of surfactant production using the drop-collapsing assay (Jain et al., 1991). To isolate the chromosomal DNA region flankin ...
Convergent evolution of antifreeze glycoproteins in
... AFGP genes are transcribed and translated into large polyproteins that are posttranslationally cleaved. The near-identical AFGP protein structures from the two fishes have led to suggestions of a common ancestor (18), and the apparent similarities in their AFGP genes appear to support such an argume ...
... AFGP genes are transcribed and translated into large polyproteins that are posttranslationally cleaved. The near-identical AFGP protein structures from the two fishes have led to suggestions of a common ancestor (18), and the apparent similarities in their AFGP genes appear to support such an argume ...
DNA sequence and chromatin structure
... Accommodate nucleosomal DNA size variation by using a variable window to match forward and reverse reads to identify position and amplitude of dyad. ...
... Accommodate nucleosomal DNA size variation by using a variable window to match forward and reverse reads to identify position and amplitude of dyad. ...
Identification of incomplete coding sequences for
... inserts from positive plaques have been isolated and subcloned into the vector pUC18 (Ballabio et al. 1987). One of these clones, p422. contains a 560bp insert. However later studies have shown that only 2(X) bp of this clone correspond to the cDN A for STS. the remaining sequence being of unknown o ...
... inserts from positive plaques have been isolated and subcloned into the vector pUC18 (Ballabio et al. 1987). One of these clones, p422. contains a 560bp insert. However later studies have shown that only 2(X) bp of this clone correspond to the cDN A for STS. the remaining sequence being of unknown o ...
Making Sense of Complicated Microarray Data
... population 1, i.e., s is significantly different from “mean 1” at the p < 0.05 significance level. But we cannot reject the hypothesis that the sample came from population 2. ...
... population 1, i.e., s is significantly different from “mean 1” at the p < 0.05 significance level. But we cannot reject the hypothesis that the sample came from population 2. ...
Promoter identification
... initiates transcription of a gene is called a promoter. • Transcription from a particular promoter is controlled by DNAbinding proteins, termed transcription factors. • DNA control elements in binding transcription factors may be located very far from the promoter they regulate. ...
... initiates transcription of a gene is called a promoter. • Transcription from a particular promoter is controlled by DNAbinding proteins, termed transcription factors. • DNA control elements in binding transcription factors may be located very far from the promoter they regulate. ...
Evolution of language: Lessons from the genome | SpringerLink
... must occur via intermediate effects on the types of neurobiological processes discussed above (proliferation, differentiation, connectivity, plasticity, etc.). The necessarily complex mappings from gene to behaviour mean that discussions that centre on an abstract “gene for language” are unconstruct ...
... must occur via intermediate effects on the types of neurobiological processes discussed above (proliferation, differentiation, connectivity, plasticity, etc.). The necessarily complex mappings from gene to behaviour mean that discussions that centre on an abstract “gene for language” are unconstruct ...
Untitled
... alternative protocol developed by US researchers. Fred’s method revolutionised genetics over the following three decades, and has only recently been superseded by modern ‘next-generation’ technology – the super-speedy high-definition Blu-ray disc to replace Fred’s clunky VHS cassette. Most of us wil ...
... alternative protocol developed by US researchers. Fred’s method revolutionised genetics over the following three decades, and has only recently been superseded by modern ‘next-generation’ technology – the super-speedy high-definition Blu-ray disc to replace Fred’s clunky VHS cassette. Most of us wil ...
Chapter 13 Lecture Notes: DNA Function I. Transcription (General
... initiate transcription via protein-protein interactions or by altering the structure of the DNA. b) Transcription of some promoters requires an accessory transcriptional activator; at other promoters, the activators just increase the rate of transcription but are not absolutely required. 3. Template ...
... initiate transcription via protein-protein interactions or by altering the structure of the DNA. b) Transcription of some promoters requires an accessory transcriptional activator; at other promoters, the activators just increase the rate of transcription but are not absolutely required. 3. Template ...
Implications of the Human Genome for Understanding Human
... now live in a world in which the 2.9 billion nucleotide codes of the human genome are available as a resource for scientific discovery. Some of the findings from the sequencing of the human genome were expected, confirming knowledge presaged by many decades of research in both human and comparative ...
... now live in a world in which the 2.9 billion nucleotide codes of the human genome are available as a resource for scientific discovery. Some of the findings from the sequencing of the human genome were expected, confirming knowledge presaged by many decades of research in both human and comparative ...
digital PCR - Bio-Rad
... copy number variable regions greater than 1 kb, harboring approximately 1,000 genes. Next-generation sequencing analyses from the 1000 Genomes Project indicated that the majority of CNVs exist as five or fewer copies per cell, but the 56 most variable gene families have median CNV values between 5 a ...
... copy number variable regions greater than 1 kb, harboring approximately 1,000 genes. Next-generation sequencing analyses from the 1000 Genomes Project indicated that the majority of CNVs exist as five or fewer copies per cell, but the 56 most variable gene families have median CNV values between 5 a ...
slides
... • Sequence preferences is over-ridden by nucleosome remodeling complexes which move them to new locations whenever needed. • Opposing view: the remodeling complexes only enable the nucleosomes to sample rapidly alternative positions and therefore compete efficiently with DNA binding proteins. They d ...
... • Sequence preferences is over-ridden by nucleosome remodeling complexes which move them to new locations whenever needed. • Opposing view: the remodeling complexes only enable the nucleosomes to sample rapidly alternative positions and therefore compete efficiently with DNA binding proteins. They d ...
pdf
... sequences (usually rRNA) and those based on the sequences of functional genes or proteins have both been demonstrated in the nitrogen cycle. The most progress has been made for ammonia oxidizers; several sets of PCR primers have been described and their specificity may be optimized to allow detectio ...
... sequences (usually rRNA) and those based on the sequences of functional genes or proteins have both been demonstrated in the nitrogen cycle. The most progress has been made for ammonia oxidizers; several sets of PCR primers have been described and their specificity may be optimized to allow detectio ...
Metagenomics
Metagenomics is the study of genetic material recovered directly from environmental samples. The broad field may also be referred to as environmental genomics, ecogenomics or community genomics. While traditional microbiology and microbial genome sequencing and genomics rely upon cultivated clonal cultures, early environmental gene sequencing cloned specific genes (often the 16S rRNA gene) to produce a profile of diversity in a natural sample. Such work revealed that the vast majority of microbial biodiversity had been missed by cultivation-based methods. Recent studies use either ""shotgun"" or PCR directed sequencing to get largely unbiased samples of all genes from all the members of the sampled communities. Because of its ability to reveal the previously hidden diversity of microscopic life, metagenomics offers a powerful lens for viewing the microbial world that has the potential to revolutionize understanding of the entire living world. As the price of DNA sequencing continues to fall, metagenomics now allows microbial ecology to be investigated at a much greater scale and detail than before.