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Article
Article

... and tissue differentiation processes, general housekeeping chores, and seed protein accumulation at appropriate embryogenic stages; more recently, gene action has been implicated in the lapse into dormancy of embryos during their final stage of development (for review: Raghavan, 1997). This paper pr ...
Mechanisms Underlying the Evolution and Maintenance of
Mechanisms Underlying the Evolution and Maintenance of

... paralogs and alleles. For example, in the case of P. vivax, the three large gene clusters correspond to at least three different paralogs. Yet, there may also be allelic sequences within some clusters (e.g., multiple ‘‘El Salvador’’ or sequences within two of the clusters). Thus, it is difficult to ...
Saccharomyces Genome Database.
Saccharomyces Genome Database.

... in other databases. The assembled resources available for a locus include links to the scientific literature (SGD's Literature Guide, a PubMed search for that locus name, and a curator-composed Gene Summary if available); several options for locus-specific DNA or protein sequence retrieval; sequence ...
[ 19] Saccharomyces Genome Database - SGD-Wiki
[ 19] Saccharomyces Genome Database - SGD-Wiki

... in other databases. The assembled resources available for a locus include links to the scientific literature (SGD's Literature Guide, a PubMed search for that locus name, and a curator-composed Gene Summary if available); several options for locus-specific DNA or protein sequence retrieval; sequence ...
Phylogenetic Affinity of Mitochondria of Euglena
Phylogenetic Affinity of Mitochondria of Euglena

... RNA molecules called guide RNAs mediate the uridine insertion/deletion type of RNA editing (Simpson et al. 1993). It is known that these guide RNA molecules can be capped in vitro with guanylyl transferase and GTP (Blum and Simpson 1990). To search for similar RNA species in E. gracilis mitochondria ...
Characterization of a heat-active archaeal β
Characterization of a heat-active archaeal β

... the nucleotide pattern. The deduced 495 amino acid comprising sequence of Bgl1 exhibited a molecular mass of 57.2 kDa and a theoretical pI of 6.3. Similarities to the glycoside hydrolase family 1 (GH1, cl01046) were detected. Conserved domains of GH1 (carbohydrate transport and mechanism, COG2723) a ...
Fine Mapping of Two Wheat Powdery Mildew Resistance Genes
Fine Mapping of Two Wheat Powdery Mildew Resistance Genes

... along with high molecular weight markers (#N0350S, New England Biolabs). Sequencing of BAC clones was performed in BGI-Shenzhen, Shenzhen, China. Gene prediction was performed using the gene ...
Fine Mapping of Two Wheat Powdery Mildew Resistance Genes
Fine Mapping of Two Wheat Powdery Mildew Resistance Genes

... along with high molecular weight markers (#N0350S, New England Biolabs). Sequencing of BAC clones was performed in BGI-Shenzhen, Shenzhen, China. Gene prediction was performed using the gene ...
Comparative study of overlapping genes in bacteria, with special
Comparative study of overlapping genes in bacteria, with special

... as a result of mutational bias towards deletion. Overlapping genes are more conserved between species than nonoverlapping genes (Lipman, 1997; Yelin et al., 2003), mostly because a mutation in the overlapping region causes changes in both genes, and selection against such mutations should therefore ...
Array CGH for detection of chromosome imbalance
Array CGH for detection of chromosome imbalance

... Array CGH analysis of DNA from XXX has been carried out using oligonucleotide arrays with ~44,000 probes across the genome. This test identified an apparently terminal deletion of approximately 84kb from band p16.3 in the short arm of chromosome 4, between base pair coordinates 72,446 and 156,159. T ...
Caspary T, Anderson KV. Dev Dyn. 2006 Sep;235(9):2412-23. Uncovering the uncharacterized and unexpected: unbiased phenotype-driven screens in the mouse. (Review)
Caspary T, Anderson KV. Dev Dyn. 2006 Sep;235(9):2412-23. Uncovering the uncharacterized and unexpected: unbiased phenotype-driven screens in the mouse. (Review)

... the past five years from a potential approach to a practical tool. This change has been driven by the relative ease of identifying causative mutations now that the complete genome sequence is available. These unbiased screens make it possible to identify genes, gene functions and processes that are ...
Laboratory Detection and Gene Cassette Stability of the
Laboratory Detection and Gene Cassette Stability of the

... blaGES-2 coding region, distinguishes this ESBL from blaGES-1 and the blaIBC-type genes, making it an ideal target for developing a novel sequence-specific, peptide nucleic acid (PNA)-based, multiplex-PCR detection method. Utilizing two primer pairs in conjunction with a PNA probe, this novel method ...
Comparative Genetic Mapping Revealed Powdery Mildew
Comparative Genetic Mapping Revealed Powdery Mildew

The Genome of a Mongolian Individual Reveals
The Genome of a Mongolian Individual Reveals

... We here also carried out the annotation of the genome draft using the pipeline developed by BGI (Li, Fan, et al. 2010). For repeats annotation, we used the RepeatModeler (1.0.5) to construct the de novo repeat library. We also used the RepeatMasker (version 3.3.0) (http://repeatmasker.org) with the ...
cis667-10
cis667-10

... • Extreme conservation of genes on X chromosomes across mammalian species provides an opportunity to study the evolutionary history of X chromosome independently of the rest of the genomes • According to Ohno´s law, the gene content of X ...
Single nucleotide polymorphisms and the future of genetic
Single nucleotide polymorphisms and the future of genetic

iMap Exercise ()
iMap Exercise ()

... functions to regulate gene expression. A colleague who is sequencing maize genomic BAC clones offers to sequence BAC clones containing some of the chromatin genes you are studying. This is terrific, because most of your genes are repesented only as cDNA sequences and it would be useful to have genom ...
Molecular Coat Colour Genetics
Molecular Coat Colour Genetics

... Coat colour, being sufficiently easy to observe and score, is a model phenotype for studying gene action and interaction. Farm animals provide us a valuable resource for identifying genes behind such phenotypic traits, because they show a remarkably higher diversity in coat colour compared to natura ...
Genetic Techniques for Biological Research Chapter4
Genetic Techniques for Biological Research Chapter4

... The first step in a genetic analysis of a process it to isolate mutant individuals that areunable to carry outthat process orcarry it out in anaberrant way. The researcher must hypothesize what characteristics, changes in growth capabilities, morphology, etc. will be exhibited by this individual and ...
Using a Single-Nucleotide Polymorphism to Predict
Using a Single-Nucleotide Polymorphism to Predict

... These fragment are 221 base pairs long. The "D" lane contains your digested PCR products. If the "D" lane contains a single band, your PCR products were not cut by the restriction enzyme HaeIII. In this case both copies of your TAS2R38 gene are identical (the same allele). This form of the gene has ...
Patchy distribution of flexible genetic elements in bacterial
Patchy distribution of flexible genetic elements in bacterial

... sympatric diversification? Answering such a question requires a clear definition of a bacterial population, a task complicated by the ecological and genetic interactions between different bacterial strains. Population definitions differ, depending on the scale at which a study of interest is conduct ...
A molecular phylogeny of enteric bacteria and implications for a
A molecular phylogeny of enteric bacteria and implications for a

... and pooled, and argue that the pooled data provides an appropriate estimate of the enteric phylogeny. In addition, this phylogeny reveals that the phenotypic ‘clusters’ that have traditionally been used to define bacterial species (Holt, 1994; Rossello-Mora & Amann, 2001) are clearly seen as gene po ...
Fine mapping of Restorer-of-fertility in pepper (Capsicum
Fine mapping of Restorer-of-fertility in pepper (Capsicum

... The majority of cloned Rf genes are members of the pentatricopeptide repeat (PPR) gene family. Through genetic mapping, co-segregation of PPR genes with Rf has also been identified in several crops including sorghum, Mimulus, and maize (CMS-S) (Barr and Fishman 2010; Klein et al. 2005; Xu et al. 200 ...
Comprehensive genetic approaches to cleft lip/palate
Comprehensive genetic approaches to cleft lip/palate

... • Our laboratory is funded in part by support from the NIDCR • There will be no discussion or endorsement of any products in this presentation ...
GNET/BIOL 621 Fall 2016 - UNC Department of Biology
GNET/BIOL 621 Fall 2016 - UNC Department of Biology

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Genome editing

Genome editing, or genome editing with engineered nucleases (GEEN) is a type of genetic engineering in which DNA is inserted, replaced, or removed from a genome using artificially engineered nucleases, or ""molecular scissors."" The nucleases create specific double-stranded break (DSBs) at desired locations in the genome, and harness the cell’s endogenous mechanisms to repair the induced break by natural processes of homologous recombination (HR) and nonhomologous end-joining (NHEJ). There are currently four families of engineered nucleases being used: Zinc finger nucleases (ZFNs), Transcription Activator-Like Effector Nucleases (TALENs), the CRISPR/Cas system, and engineered meganuclease re-engineered homing endonucleases.It is commonly practiced in genetic analysis that in order to understand the function of a gene or a protein function one interferes with it in a sequence-specific way and monitors its effects on the organism. However, in some organisms it is difficult or impossible to perform site-specific mutagenesis, and therefore more indirect methods have to be used, such as silencing the gene of interest by short RNA interference (siRNA) . Yet gene disruption by siRNA can be variable and incomplete. Genome editing with nucleases such as ZFN is different from siRNA in that the engineered nuclease is able to modify DNA-binding specificity and therefore can in principle cut any targeted position in the genome, and introduce modification of the endogenous sequences for genes that are impossible to specifically target by conventional RNAi. Furthermore, the specificity of ZFNs and TALENs are enhanced as two ZFNs are required in the recognition of their portion of the target and subsequently direct to the neighboring sequences.It was chosen by Nature Methods as the 2011 Method of the Year.
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