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ppt_II
ppt_II

... Automatically finds genes and other features of the sequence Associates sequence and features with data from other sources Provides a publicly accessible web based interface to the database ...
DNA helicase deficiencies associated with cancer
DNA helicase deficiencies associated with cancer

... DNA damage exists in many different forms and can arise as a result of defects in any aspect of DNA metabolism. To deal with DNA damage, all cells have developed a range of responses, which include pathways for DNA repair and arrest of the cell cycle (1,2). Mutations in DNA repair genes frequently l ...
A homologue of the breast cancer associated gene BARD1 is
A homologue of the breast cancer associated gene BARD1 is

... similar to BRCA1 (Wu et al, 1996). As further studies demonstrated that both proteins are able to form a heterodimer through their common N-terminal RING domain, this protein was designated BARD1, breast cancer associated RING domain (Meza et al, 1999; Joukov et al, 2001). This hBRCA1/ hBARD1 hetero ...
Chapter 8 - Human Genetics and Biotechnology
Chapter 8 - Human Genetics and Biotechnology

... Linkage can be assessed by determining how often crossing-over occurs between two genes on the same chromosome. Genes on different (nonhomologous) chromosomes are not linked. They assort independently during meiosis, so they have a 50 percent chance of ending up in different gametes. If genes show up ...
Sequenced Mitochondrial Genomes of Bryophytes
Sequenced Mitochondrial Genomes of Bryophytes

... and biochemical functions related to their bacterial ancestors. During evolution a massive loss/transfer of mitochondrial genes to the host genome occurs consequently mitochondria harbors a very small fraction of the endosymbiont's genome. Moreover they are highly diversified in different phyla of p ...
Using the Basic Local Alignment Search Tool (BLAST) - bio-bio-1
Using the Basic Local Alignment Search Tool (BLAST) - bio-bio-1

... a minimization procedure. The SEG program is used for analysis of either proteins or nucleic acids by the above methods. PSEG and NSEG are similar to SEG but are set up for analysis of protein and nucleic acid sequences, respectively. These versatile programs may also be used for locating specific s ...
PRESENTATION No
PRESENTATION No

... of proteins that putatively include from about 15 up to 30% of the proteome of organisms fully sequenced. These estimates are mainly computed on the basis of sequence comparison and membrane protein prediction. It is therefore urgent to develop methods capable of selecting membrane proteins especial ...
New New Developments Gene Therapy
New New Developments Gene Therapy

... Gene therapy is a difficult and complex process which is currently in an early stage of development and is used in the treatment of a limited number of medical conditions. Protein therapy has been successful for many medical problems such as Pompe disease. Originally, the particular therapeutic prot ...
File
File

... soft, spongy bread rather than a firm, thin cracker, they were acting as fledgling biotechnologists.  The first animal breeders, realizing that different physical traits could be either magnified or lost by mating appropriate pairs of animals, engaged in the manipulations of biotechnology. ...
History of Biotech and Biotech Applications
History of Biotech and Biotech Applications

... soft, spongy bread rather than a firm, thin cracker, they were acting as fledgling biotechnologists.  The first animal breeders, realizing that different physical traits could be either magnified or lost by mating appropriate pairs of animals, engaged in the manipulations of biotechnology. ...
overview - El Paso High School
overview - El Paso High School

... DNA replication begins with the binding of a large protein complex—the pre-replication complex—to a specific site on the DNA molecule. The complex contains DNA polymerase, which catalyzes addition of nucleotides. The complex binds to a region on the chromosome called the origin of replication (ori). ...
video slide - Buena Park High School
video slide - Buena Park High School

... Transposase gene (b) Transposons contain one or more genes in addition to the transposase gene. In the transposon shown here, a gene for resistance to an antibiotic is located between twin insertion sequences. The gene for antibiotic resistance is carried along as part of the transposon when the tra ...
Analysis by pulsed-field gel electrophoresis mutations in the
Analysis by pulsed-field gel electrophoresis mutations in the

... loci, although three of the five loci were located on the same 330-kb SmaI fragment of the wild-type strain Eagan chromosome. This fragment also contains several important virulence determinants, including the capb locus, and one of the five constitutive mutants had concomitantly lost the ability to ...
GMOD: Identifying Genetically Modified Organisms in Food
GMOD: Identifying Genetically Modified Organisms in Food

... • After isolating DNA from samples of food, you will use PCR to amplify two genes. • The first gene, known as NPTII, is part of the vector used to create many GMOs. • The second gene is a 16S ribosomal gene associated with plant chloroplast DNA. Because this gene is present in all plants, it serves ...
The Ingredients for a Postgenomic Synthesis of Nature and Nurture
The Ingredients for a Postgenomic Synthesis of Nature and Nurture

... stretch of DNA. Beyond the activation of DNA an ever-expanding array of processing and targeting mechanisms are coming into play that not only determine the final gene product but which amplify the repertoire of protein products specified through the eukaryotic genome. We have to understand that gen ...
Genetic Linkage Analysis
Genetic Linkage Analysis

... AAAAAAAAAAA would be referred to as (A) 11 GTGTGTGTGTGT would be referred to as (GT) 6 CTGCTGCTGCTG would be referred to as (CTG) 4 ACTCACTCACTCACTC would be referred to as (ACTC) 4 ...
Document
Document

... Copyright © 2005 by author, who wishes to thank Dr. Raymond F. Gesteland for his editorial assistance. ...
Powerpoint for Bioinformatics course at CSULA
Powerpoint for Bioinformatics course at CSULA

... Introduce software and databases currently used by bioinformaticists Introduce how data is organized. How data is gathered and how it is annotated. Introduce statistics of data analysis. Introduce the concept of dynamic programming Give an opportunity to create an algorithm that analyzes sequence da ...
Transposition - Pennsylvania State University
Transposition - Pennsylvania State University

... Evidence Mechanisms: DNA-mediated RNA-mediated ...
Genetic markers in beef and sheep breeding
Genetic markers in beef and sheep breeding

... those animals that carry either specific alleles or the characteristic markers associated with those alleles means that we can now identify animals with the ‘best’ genes for some of the complex traits more accurately. The large scale breeding programmes and recording of millions of animals in contro ...
Sample pages 2 PDF
Sample pages 2 PDF

... In the process of transcription, some sections of DNA, called genes, are transcribed into complementary copies of ribonucleic acid (RNA). Since RNA is single stranded, only one strand of DNA is used in the transcription process. The resulting RNA strand is complementary and antiparallel to the “pare ...
ppt - Chair of Computational Biology
ppt - Chair of Computational Biology

... A much more expanded view of epigenetics has recently emerged ...
2 Genetic Epidemiology - How to quantify, localize and identify
2 Genetic Epidemiology - How to quantify, localize and identify

... dominance can be found on http://pngu.mgh.harvard.edu/~purcell/bgim/index2.html#sgene. In this chapter we will provide an overview of genetic epidemiological methods and developments, in three sections. The first part will describe the estimation of heritability, as well as some more advanced modeli ...
De novo Structure Variations of the Y Chromosome in a 47,XXY
De novo Structure Variations of the Y Chromosome in a 47,XXY

... was then used to detect the CNVs in the genome of the patient. Two large deletions were identified on the short arm of the Y chromosome (fig. 1c). Deletion 1 was ∼6 Mb in size and spanned Yp11.32 to Yp11.2 (positions from the gene chip data: 179,542–6,110,498 bp; hg19) and included the genes ZBED1, ...
Dihydropyrimidine dehydrogenase (DPD)
Dihydropyrimidine dehydrogenase (DPD)

... Fluorouracil is mainly (> 80%) converted by dihydropyrimidine dehydrogenase (DPD) to the inactive metabolite dihydrofluorouracil. DPD is mainly present in the liver, but also in most other tissues. Lower metabolic activity of DPD leads to increased intracellular concentrations of fluorodeoxyuridine ...
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Genome editing

Genome editing, or genome editing with engineered nucleases (GEEN) is a type of genetic engineering in which DNA is inserted, replaced, or removed from a genome using artificially engineered nucleases, or ""molecular scissors."" The nucleases create specific double-stranded break (DSBs) at desired locations in the genome, and harness the cell’s endogenous mechanisms to repair the induced break by natural processes of homologous recombination (HR) and nonhomologous end-joining (NHEJ). There are currently four families of engineered nucleases being used: Zinc finger nucleases (ZFNs), Transcription Activator-Like Effector Nucleases (TALENs), the CRISPR/Cas system, and engineered meganuclease re-engineered homing endonucleases.It is commonly practiced in genetic analysis that in order to understand the function of a gene or a protein function one interferes with it in a sequence-specific way and monitors its effects on the organism. However, in some organisms it is difficult or impossible to perform site-specific mutagenesis, and therefore more indirect methods have to be used, such as silencing the gene of interest by short RNA interference (siRNA) . Yet gene disruption by siRNA can be variable and incomplete. Genome editing with nucleases such as ZFN is different from siRNA in that the engineered nuclease is able to modify DNA-binding specificity and therefore can in principle cut any targeted position in the genome, and introduce modification of the endogenous sequences for genes that are impossible to specifically target by conventional RNAi. Furthermore, the specificity of ZFNs and TALENs are enhanced as two ZFNs are required in the recognition of their portion of the target and subsequently direct to the neighboring sequences.It was chosen by Nature Methods as the 2011 Method of the Year.
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