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SEG exam 2 1
SEG exam 2 1

Genetics Study Guide
Genetics Study Guide

... 7. What is genetic engineering used for? Genetically alter plants and manufacture proteins 8. Some genetic disorders, such as sickle cell anemia, are due to a mutation. 9. Nucleotides are made of a sugar, a phosphate, and a base. 10. What is a phenotype? The way an organism looks 11. A string of nuc ...
Using restriction enzymes, foreign genes can be added to an
Using restriction enzymes, foreign genes can be added to an

DNA - heredity2
DNA - heredity2

... DNA Profiling • Approximately 5% of your DNA codes for proteins • The other ~95% is non-coding or ‘junk’ DNA which varies greatly between individuals • In this ‘junk’ there are sections which have repeated patterns • These repeated patterns are what is used to identify an individual when doing DNA ...
Deoxyribonucleic Acid (DNA)
Deoxyribonucleic Acid (DNA)

Key Concepts File - Northwest ISD Moodle
Key Concepts File - Northwest ISD Moodle

... cells). Eukaryotic multicellular organisms reproduce sexually by combining two gametes containing homologous chromosomes (one set of chromosomes from each parent) during fertilization. Crossing over during meiosis allows for the reshuffling of genetic combinations between individual homologous chrom ...
Finding Protein-Coding Genes
Finding Protein-Coding Genes

... The purpose of this exercise is to illustrate some of the concepts in the lectures and readings by using web servers to annotate genes. As with all my assignments, if your interests lead you in a different direction, you are free to follow that direction as long as it deals with gene annotation. You ...
Deoxyribonucleic Acid (DNA)
Deoxyribonucleic Acid (DNA)

... Deoxyribonucleic Acid (DNA) Deoxyribonucleic acid (DNA) is the chemical compound that contains the instructions needed to develop and direct the activities of nearly all living organisms. DNA molecules are made of two twisting, paired strands, often referred to as a double helix. Each DNA strand is ...
How can we tell synthetic from native sequences?
How can we tell synthetic from native sequences?

... maximize difference (Avoid first 100 bases of each gene) At least 33% of nucleotides recoded (target tags to regions where amino acids can vary at >1 nucleotide) First and last nucleotides correspond to variable position Melting temperature between 58-60C Amplifies 200-500 bp fragment Primers will n ...
Biotechnology and Recombinant DNA
Biotechnology and Recombinant DNA

... the specific gene of interest – Can be difficult (number of cells) ...
Assignment #1
Assignment #1

... during cell division to produce gametes(6) containing one chromosome of each type. b. Only certain cells in a multicellular(7) organism undergo meiosis. c. Random chromosome segregation explains the probability that a particular allele(8) with be in a gamete(9). d. e. Approximately half of an indivi ...
1 - web.biosci.utexas.edu
1 - web.biosci.utexas.edu

... d. degradation of the transposon while it is moving 7. Oxidative stress can damage DNA by a. causing single-strand breaks b, causing double-strand breaks c. oxidation of guanine to 8-oxo-guanine d. b and c e. all of the above 8. Which of the following is not true regarding DNA photolyases a. repair ...
LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

... 19. Expand MODY. Which is the most commonly seen MODY phenotype in India? 20. What is the function of Cytochrome P450 gene (CYP450)? ...
Answer Key DNA Review - John Bowne High School
Answer Key DNA Review - John Bowne High School

... 23. Coded instructions that arc passed from one generation to the next can be most directly changed by the processes of A) passive transport, natural selection, and synthesis B) selective breeding, replication, and absorption C) recombination, mutation, and genetic engineering D) evolution, reproduc ...
Week 10 Pre-Lecture Slides
Week 10 Pre-Lecture Slides

Human Genome Project and Sequencing
Human Genome Project and Sequencing

... (though Dr. Venter’s DNA was “randomly” selected ...
Population Genetics Sequence Diversity Molecular Evolution
Population Genetics Sequence Diversity Molecular Evolution

... How common are polymorphisms ? - a debate in the 1950s How much genetic variation within a species Classical school concern about genetic load - most mutations are expected to be deleterious H.J. Muller predicted that only one locus (protein) in 1000 would be polymorphic J.B.S. Haldane thought rate ...
No patents on Life - Diakonia Council Of Churches
No patents on Life - Diakonia Council Of Churches

... There are a few ways in which genetic engineers force the foreign gene cassette into the host plant cell: 1. Certain bacteria (Agrobacterium tumefaciens) normally infect plants by inserting a portion of its own DNA into a plant, which causes the plant to grow tumours. Gene scientists swop the tumour ...
File
File

... a) some may carry oncogenes- genes to send cell cycle out of control b) some viruses may turn on protooncogenes at inappropriate times ...
DNA Discovery
DNA Discovery

... Recombinant DNA Recombinant DNA •Bacteria often provide the appropriate machinery (enzymes and ribosomes) for us to produce proteins from a specific gene  insulin •Bacteria have small circular pieces of DNA called plasmids within their cytoplasm ...
BIOTECHNOLOGY AND GENETIC ENGINEERING
BIOTECHNOLOGY AND GENETIC ENGINEERING

... experiment on all stem cells?  Experimenting with adult stem cells is not controversial because it does not harm the adult if the cells are removed from the body.  There has been some exciting recent research that has demonstrated the ability to turn adult stem cells into embryo-like stem cells. T ...
Gene expression and DNA microarrays
Gene expression and DNA microarrays

... (common lab strain) found that the O157:H7 genome is ~ 1Mb larger than K-12 and contains 1,387 genes specific for O157:H7. – Genomes share a 4.1 Mb backbone with species specific DNA interspersed throughout the genome • K-islands - specific to K-12 (0.53Mb) • O-islands - specific ...
ASviewer: Visualizing the transcript structure and functional
ASviewer: Visualizing the transcript structure and functional

Genetics and Our Lives
Genetics and Our Lives

... Clones are genetically identical. Plants clone easily (cuttings), but animal cells are much more difficult. Dolly the sheep (first mammal cloned). Identical twins are the only human clones. ...
Recombinant DNA - University of Central Oklahoma
Recombinant DNA - University of Central Oklahoma

... be passed on to future generations, causing irreversible gene pollution and the potential for new genetic diseases. • In addition to the immediate and long-term gross health risks posed by irreversible gene pollution, we have no idea what the subtle effects of incomplete or mutated human DNA will be ...
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Genome editing

Genome editing, or genome editing with engineered nucleases (GEEN) is a type of genetic engineering in which DNA is inserted, replaced, or removed from a genome using artificially engineered nucleases, or ""molecular scissors."" The nucleases create specific double-stranded break (DSBs) at desired locations in the genome, and harness the cell’s endogenous mechanisms to repair the induced break by natural processes of homologous recombination (HR) and nonhomologous end-joining (NHEJ). There are currently four families of engineered nucleases being used: Zinc finger nucleases (ZFNs), Transcription Activator-Like Effector Nucleases (TALENs), the CRISPR/Cas system, and engineered meganuclease re-engineered homing endonucleases.It is commonly practiced in genetic analysis that in order to understand the function of a gene or a protein function one interferes with it in a sequence-specific way and monitors its effects on the organism. However, in some organisms it is difficult or impossible to perform site-specific mutagenesis, and therefore more indirect methods have to be used, such as silencing the gene of interest by short RNA interference (siRNA) . Yet gene disruption by siRNA can be variable and incomplete. Genome editing with nucleases such as ZFN is different from siRNA in that the engineered nuclease is able to modify DNA-binding specificity and therefore can in principle cut any targeted position in the genome, and introduce modification of the endogenous sequences for genes that are impossible to specifically target by conventional RNAi. Furthermore, the specificity of ZFNs and TALENs are enhanced as two ZFNs are required in the recognition of their portion of the target and subsequently direct to the neighboring sequences.It was chosen by Nature Methods as the 2011 Method of the Year.
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