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SEARCH_16S: A new algorithm for identifying 16S
SEARCH_16S: A new algorithm for identifying 16S

... To test the sensitivity of SEARCH_16S I used all finished prokaryotic genomes in Genbank and three large curated databases containing small subunit (SSU) ribosomal sequences: SILVA (Pruesse et al., 2007) v23, RDP (Maidak et al., 2001) downloaded 30th Sept. 2016 and Greengenes v13.5. I identified the ...
- Philsci
- Philsci

... ancestry, but are instead used to identify the historical events which led to the homology within an organism, in terms of the gene duplication events. Homology of one type does not necessitate similarity of another type. But why is it that the phylogenetic lineage of a gene or genome cannot merely ...
DNA vaccines against influenza
DNA vaccines against influenza

... generation” vaccines against influenza virus. Subunit vaccines and DNA vaccines against influenza virus are often based on hemagglutinin (HA), the main viral antigen. Other viral proteins are used rather rarely, usually together with HA, however the sequences encoding NA, M2, NP and M1 were also use ...
Somatic MEN1 gene mutation does not contribute
Somatic MEN1 gene mutation does not contribute

... The pathogenesis of pituitary tumours remains unexplained in most cases. The MEN1 gene has been cloned recently. As pituitary tumours are classically associated with MEN1, we tested the presence of MEN1 gene mutations in sporadic tumours. We studied 35 secreting and non-secreting sporadic adenomas. ...
Activation of proto-oncogenes by disruption of
Activation of proto-oncogenes by disruption of

... LMO2 as evidenced by 5C analysis (Fig. 3K-L, S10). These results indicate that the deleted CTCF sites contribute to the silent state of the LMO2 proto-oncogene (Fig. 3J). ...
Presence of a mitochondrial-type 70
Presence of a mitochondrial-type 70

... mainly on ribosomal RNA, show that three amitochondriate protist lineages, diplomonads, microsporidia, and trichomonads, emerge consistently at the base of the eukaryotic tree before groups having mitochondria. This suggests that these groups could have diverged before the mitochondrial endosymbiosi ...
Towards an accurate identification of mosaic genes and partial
Towards an accurate identification of mosaic genes and partial

... imposed by the necessity for taxa involved in HGT to be contemporaneous (6,18,20). Fix the sliding window size w and the step size s. In our experiments, the window sizes of l/5, l/4, l/3 and l/2 sites and the sliding window progress step of 10 sites were used. Step k. Fix the position of the slidin ...
Gill: Human Disease Genomics
Gill: Human Disease Genomics

Powerpoint summary
Powerpoint summary

... Biotech Time Line Humans domesticate crops and livestock ...
Comparative In silico Study of Sex
Comparative In silico Study of Sex

... and 4) classified the species into two groups. Group 1 contains of four species (Homo sapiens, Pan troglodytes, Rattusnorvegicus, and Musmusculus) with the lowest genetic distances, Group 2 contains11 species (Canis lupus, Tursiopsaduncus, Susscrofa, ...
Application Note: Targeted sequencing and chromosomal haplotype
Application Note: Targeted sequencing and chromosomal haplotype

... complete loci on the basis of crosslinking physically proximal sequences. Unlike other targeted sequencing methods, TLA works without prior detailed locus information, as one primer pair is sufficient to amplify tens to hundreds of kilobases of DNA surrounding that locus. In a separate application o ...
The California Institute for Telecommunications and
The California Institute for Telecommunications and

... Dept. of Computer Science and Engineering Jacobs School of Engineering, UCSD ...
The Novel Gene HOMOLOGOUS PAIRING
The Novel Gene HOMOLOGOUS PAIRING

... little effect on bouquet formation (Pawlowski et al., 2004). The AHP2 of Arabidopsis (Arabidopsis thaliana) is homologous to Saccharomyces cerevisiae HOP2 (Leu et al., 1998) and S. pombe MEU13 (Nabeshima et al., 2001), which are known to act in monitoring homology between pairing partner at meiosis, ...
The Novel Gene HOMOLOGOUS PAIRING
The Novel Gene HOMOLOGOUS PAIRING

... phs1 mutation of maize causes nonhomologous synapsis, delay of DNA double-strand break (DSB) repair, and dramatically reduced RAD51 foci on meiotic chromosomes, although it has little effect on bouquet formation (Pawlowski et al., 2004). The AHP2 of Arabidopsis (Arabidopsis thaliana) is homologous t ...
Genetic Analysis of Familial Connective Tissue Alterations
Genetic Analysis of Familial Connective Tissue Alterations

... Three hundred and eighty-three autosomal microsatellite markers of the Applied Biosystems MD10 linkage mapping set (mean distance of the genetic markers 10 cM) were used for an initial genome scan of family A. Fluorescent amplification products were visualized on the ABI-310 genetic analyzer and ana ...
13-3
13-3

... vary widely. Some have little or no effect; and some produce beneficial variations. Some negatively disrupt gene function. Many if not most mutations are neutral; they have little or no effect on the expression of genes or the function of the proteins for which they code. Whether a mutation is negati ...
Identifying 3D expression domains by graph clustering
Identifying 3D expression domains by graph clustering

... The five types labeled in the work are identified as major types which may consist of subtypes.  Each type is defined by unique combinations of proteins, with proteins not restricted to a single  type of chromatin. One of the main findings is that roughly half the genome is covered by a  repressive  ...
The nuclear envelope in genome organization, expression and
The nuclear envelope in genome organization, expression and

... contains recombination-enhancing (RE) sequences that harbour replication fork-blocking sequences, which can stall replication forks and induce double strand breaks. The locations of the centromere (Cen), telomeres (Tel), Pol I transcription initiation region (TIR) and DNA replication origin (empty c ...
File
File

... vary widely. Some have little or no effect; and some produce beneficial variations. Some negatively disrupt gene function. Many if not most mutations are neutral; they have little or no effect on the expression of genes or the function of the proteins for which they code. Whether a mutation is negat ...
Co-dominant SCAR marker for detection of the begomovirus
Co-dominant SCAR marker for detection of the begomovirus

... one of 3 nt, and one of 120 nt). The sequence for the T0302 marker from S. habrochaites LA0386 (909 bp, EU046611) was identical to the sequence from H24. The sequence for the T0302 marker (789 bp, EU046612) was also obtained for another accession, S. chilense LA2779, that has been used as a source ...
Genes for Two Mitochondrial Ribosomal Proteins in
Genes for Two Mitochondrial Ribosomal Proteins in

... the mitochondrial genome of the liverwort Marchantia polymorpha that are not present in the mitochondrion of Arabidopsis or any other angiosperm examined. rpl6 has been identified in the Arabidopsis nucleus (Arabidopsis Genome Initiative, 2000), but a transferred rps8 gene has not been ...
SCOOTER OER Fact Sheet: Dr V Rolfe, December 2010
SCOOTER OER Fact Sheet: Dr V Rolfe, December 2010

... How many chromosomes are there in a human cell? ...
The nucleotide sequence of Saccharomyces cerevisiae
The nucleotide sequence of Saccharomyces cerevisiae

... opposite strands without any intervening nucleotide between the stop codons11. This region, as well as the CSE1 region, have been verified by direct PCR sequencing on the yeast genome. Chromosome VII contains six yeast retrotransposons: three Ty1s, one Ty2, one Ty3 and a pseudo-Ty, which contains, i ...
Genome Visualisation and Annotation Tools: Artemis and ACT
Genome Visualisation and Annotation Tools: Artemis and ACT

... Feature. A window will appear containing all the annotation that is associated with that CDS. The format for this information is constrained by that which can be submitted to the EMBL database where it is stored within ‘keys’ and ‘Qualifiers’ see Appendix II. Viewing amino acid or protein sequence C ...
Correction of copy number induced false positives in
Correction of copy number induced false positives in

< 1 ... 33 34 35 36 37 38 39 40 41 ... 445 >

Genome editing

Genome editing, or genome editing with engineered nucleases (GEEN) is a type of genetic engineering in which DNA is inserted, replaced, or removed from a genome using artificially engineered nucleases, or ""molecular scissors."" The nucleases create specific double-stranded break (DSBs) at desired locations in the genome, and harness the cell’s endogenous mechanisms to repair the induced break by natural processes of homologous recombination (HR) and nonhomologous end-joining (NHEJ). There are currently four families of engineered nucleases being used: Zinc finger nucleases (ZFNs), Transcription Activator-Like Effector Nucleases (TALENs), the CRISPR/Cas system, and engineered meganuclease re-engineered homing endonucleases.It is commonly practiced in genetic analysis that in order to understand the function of a gene or a protein function one interferes with it in a sequence-specific way and monitors its effects on the organism. However, in some organisms it is difficult or impossible to perform site-specific mutagenesis, and therefore more indirect methods have to be used, such as silencing the gene of interest by short RNA interference (siRNA) . Yet gene disruption by siRNA can be variable and incomplete. Genome editing with nucleases such as ZFN is different from siRNA in that the engineered nuclease is able to modify DNA-binding specificity and therefore can in principle cut any targeted position in the genome, and introduce modification of the endogenous sequences for genes that are impossible to specifically target by conventional RNAi. Furthermore, the specificity of ZFNs and TALENs are enhanced as two ZFNs are required in the recognition of their portion of the target and subsequently direct to the neighboring sequences.It was chosen by Nature Methods as the 2011 Method of the Year.
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