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Manipulating DNA extracting and studying DNA
Manipulating DNA extracting and studying DNA

... A defective protein is replaced with a good one, eliminating the symptoms of the disease. Insertion of a new “healthy” gene into the organism to provide needed (usually) proteins, hormones etc. Gene is carried into the host by a viral vector (like the flu virus) that has been disabled. Can provide r ...
How Genes and Genomes Evolve
How Genes and Genomes Evolve

Geneticist Definition of Gene
Geneticist Definition of Gene

Chapter 20 Terms to Know
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... Insulin is made by the pancreas.  This peptide hormone (protein) ensures that glucose is taken up by the ...
Recombination, Lateral Gene Transfer, and Gene Duplication Can
Recombination, Lateral Gene Transfer, and Gene Duplication Can

... We have see the tree of life branching as new adaptations and specialization occurs within individual organisms, however there are processes that can result in lateral gene transfer Lateral gene transfer—individual genes, organelles, or genome fragments move horizontally from one lineage to another ...
8th Grade Unit Plan: Genetics
8th Grade Unit Plan: Genetics

... Individual conferences during work time: I try to meet with each student individually at least once per work period (work time in lesson) Peer-editing: students switch work and check their partner’s accuracy. Students use peer editing form to provide specific feedback for their classmate. Informed I ...
Information- Part 1 Study Guide
Information- Part 1 Study Guide

... (A) Viruses have highly efficient replicative capabilities that allow for rapid evolution and acquisition of new phenotypes. (B) Viruses replicate via a component assembly model allowing one virus to produce many progeny simultaneously via the lytic cycle. (C) Virus replication allows for mutations ...
DNA Typing
DNA Typing

... Identifying the gene associated with a specific disease requires years of work. The first step is to identify the region of the chromosome the gene is in (pedigree analysis, identifying breaks in chromosomes which cause the disease, etc.) Once the gene has been localized to a region of a chromosome, ...
Homologous Recombination DNA break repair by homologous
Homologous Recombination DNA break repair by homologous

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No Slide Title
No Slide Title

... - Gene is inserted into plasmid - Plasmid is transformed into a host cell (E. coli) - Cell culture is prepared - Each cell contains several copies of the plasmid with gene - Gene expression leads to the production of protein - Protein level may reach 30% of total cellular protein -Isolation of prote ...
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... MDLRQFLMCLSLCTAF I ordered this gene to contain an EcoR1 site at the 5’ end of the coding sequence. Show precisely where M starts by circling the correct peaks (1pt) Please determine if the sequencing is correct? (1pt) Yes or No (circle one) What amino acid follows the 2nd F in the sequence below? ( ...
Biology: Unit 13 Directed Reading Guide
Biology: Unit 13 Directed Reading Guide

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Genetics and Biotechnology Test Review

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Name _________KEY___________________________
Name _________KEY___________________________

... 35. What is the goal of the Human Genome Project? Map (find the location of) the genes on human chromosomes 36. What is genetic engineering? Manipulating genes for practical purposes 37. Describe how bacteria are used to produce human genes. (ex insulin) 1) DNA is cut in human DNA and bacterial plas ...
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Lecture 6 Gene expression: microarray and deep sequencing

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stranded DNA from genomic library

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Ch. 13 - Genetic Engineering
Ch. 13 - Genetic Engineering

... the cell, the external DNA gets incorporated into the bacterium’s own DNA. Recombinant DNA has been made.  The cell has been transformed. It will make a new protein(s). ...
statistical testing
statistical testing

... Our group is heavily involved in the analysis and interpretation of deep sequencing data. During 2010 we have implemented several pipelines capable of analyzing different types of data such as RNA-seq, resequencing data for polymorphism detection, De novo assembly , ChIP-Seq and functional annotatio ...
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Thalassaemia

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Genetic conditions - Centre for Genetics Education
Genetic conditions - Centre for Genetics Education

... are found in pairs and each pair varies in size. Thus there are 23 pairs of chromosomes, one of each pair being inherited from each parent. ...
key words for genetics
key words for genetics

... One of the phenotypes you will learn about when 23andMe genotypes you is whether you can taste a bitter flavor in raw broccoli. Some people’s tongue cells make a protein that can detect bitter flavors; others make one that can’t. Each of your cells contains a copy of your genome, which is made up of ...
Biotech
Biotech

... • A way to get genes into bacteria easily – insert new gene into plasmid – insert plasmid into bacteria = vector – bacteria now expresses new gene • bacteria make new protein gene from other organism ...
Biology 303 EXAM II 3/14/00 NAME
Biology 303 EXAM II 3/14/00 NAME

... 1. from one cell into the culture medium, where it is taken up by another cell. 2. with the help of a viral go-between. 3. in a bidirectional fashion between two cells. 4. from one bacterium to another. ...
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... • Both female and male organisms have identical chromosomes except for one pair. • Genes are located on chromosomes • All organisms have two types of chromosomes: • Sex chromosomes ...
Mendelian Genetics
Mendelian Genetics

... • evaluate the possible impact of biotechnology on the individual, society, and the environment, including medical and ethical issues, such as: ...
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Genome editing

Genome editing, or genome editing with engineered nucleases (GEEN) is a type of genetic engineering in which DNA is inserted, replaced, or removed from a genome using artificially engineered nucleases, or ""molecular scissors."" The nucleases create specific double-stranded break (DSBs) at desired locations in the genome, and harness the cell’s endogenous mechanisms to repair the induced break by natural processes of homologous recombination (HR) and nonhomologous end-joining (NHEJ). There are currently four families of engineered nucleases being used: Zinc finger nucleases (ZFNs), Transcription Activator-Like Effector Nucleases (TALENs), the CRISPR/Cas system, and engineered meganuclease re-engineered homing endonucleases.It is commonly practiced in genetic analysis that in order to understand the function of a gene or a protein function one interferes with it in a sequence-specific way and monitors its effects on the organism. However, in some organisms it is difficult or impossible to perform site-specific mutagenesis, and therefore more indirect methods have to be used, such as silencing the gene of interest by short RNA interference (siRNA) . Yet gene disruption by siRNA can be variable and incomplete. Genome editing with nucleases such as ZFN is different from siRNA in that the engineered nuclease is able to modify DNA-binding specificity and therefore can in principle cut any targeted position in the genome, and introduce modification of the endogenous sequences for genes that are impossible to specifically target by conventional RNAi. Furthermore, the specificity of ZFNs and TALENs are enhanced as two ZFNs are required in the recognition of their portion of the target and subsequently direct to the neighboring sequences.It was chosen by Nature Methods as the 2011 Method of the Year.
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