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Slide 1
Slide 1

... followed by synthesis of a new DNA strand • Damage recognized by endonuclease  cut phosphodiester backbone on both sides of damage • Excision of DNA with damaged backbone carried out by exonuclease ...
Recombinant DNA
Recombinant DNA

... Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings ...
Analyzing DNA Sequence Similarity on the Computer
Analyzing DNA Sequence Similarity on the Computer

... Part C: Creating a phylogenetic tree based on the genomic information for this gene of interest Now that you know this gene is one that is conserved across multiple species, you can use compare your DNA sequence to the sequences of other species in order to create a phylogenetic tree that shows just ...
Name Date Period ______ Chapter 3 and 4 Study Points Discuss
Name Date Period ______ Chapter 3 and 4 Study Points Discuss

... DNA is composed of sugars, phosphates, and nitrogen bases. The nitrogen bases pair up (A with T and T with A) (C with G and G with C). In DNA replication, an enzyme unzips the DNA, free nitrogen bases with sugars and phosphates attached find their match, and an enzyme zips them back up. The result i ...
Common Assessment Review
Common Assessment Review

... Transcription- process by which RNA is made. Part of the nucleotide sequence of a DNA molecule is copied into RNA. Occurs in the nucleus Steps: DNA is unzipped in the nucleus by an enzyme - Another enzyme, RNA polymerase, bonds “free nucleotides” to the exposed bases - Adenine bonds with uracil (A ...
Advances in Genetics
Advances in Genetics

... Genetic Engineering In Practice: Insulin ...
Biotech Mini-Lab Students will model the process of using restriction
Biotech Mini-Lab Students will model the process of using restriction

... 1. Cut out the plasmid strips along the dotted lines. Connect the strips and tape them together to form a single long strip. Letters should all be in the same direction when the strips are taped. The two ends of the strip should then be taped together with the genetic code facing out to form a circu ...
Chapter 13: The Molecular Basis of Inheritance
Chapter 13: The Molecular Basis of Inheritance

Mutations - The Super Heroes of Biology
Mutations - The Super Heroes of Biology

... • One nucleotide is replaced by another but it still codes for the same amino acid ...
Electrical Biosensors in Microfluidic for High Throughput Genomics and Proteomics   
Electrical Biosensors in Microfluidic for High Throughput Genomics and Proteomics   

... Department of Electrical Engineering  ...
Genetic Engineering
Genetic Engineering

Modern Genetics Meets the Dodo and the Solitaire
Modern Genetics Meets the Dodo and the Solitaire

... are inherited and can be followed through different generations of a family by using a pedigree. Other genetic disorders, such as cancer, are caused by mutations that occur during a person’s lifetime. ...
Single Nucleotide Polymorphism
Single Nucleotide Polymorphism

Test Answers - WordPress.com
Test Answers - WordPress.com

... would be affected. Person III-1 is unaffected. All other modes of inheritance can be justified from this
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AP Biology Review Sheet for Chapters 18,19, and 20 Test (Test on
AP Biology Review Sheet for Chapters 18,19, and 20 Test (Test on

... Chapter 19 Vocabulary and objectives Chromatin ...
Genome Annotation: From Sequence to Biology
Genome Annotation: From Sequence to Biology

... -Assign putative functions to proteins of an organism -Classify proteins into families: -using similarities to better-characterized proteins of other species (BLASTP) -on the basis of functional domains, motifs, and folds -Search against protein databases of functional domains (e.g. PFAM) -InterPro: ...
39 Karyotyping and Chromosomes Discovering
39 Karyotyping and Chromosomes Discovering

... Page 3 (Cont. #33 Bio. B) treatments for humans (most human insulin is made by bacteria today). In the past, we succeeded in breeding certain traits into animals (larger cattle, cows that give more milk) by selective breeding. Selective breeding can be when you specifically mate a particular animal ...
Biologists have learned to manipulate DNA
Biologists have learned to manipulate DNA

... 2. Help us understand how our genes work from others 13.2 Biologists can engineer bacteria to make useful products I. Engineering bacteria: an introduction A. Plasmids are small circle-shape DNA molecule separate from larger bacterial chromosomes B. Plasmids can be shared between bacteria, for examp ...
BIO208 Bacterial Genetics Worksheet 1 1. . Fill in: Transformation
BIO208 Bacterial Genetics Worksheet 1 1. . Fill in: Transformation

... a. the reciprocal exchange of DNA between two DNA fragments of identical (or at least close to identical) sequences is called __________________________________ b. A plasmid can be maintained _______________________ or integrate into bacterial chromosome. c. In ______________ transduction, all bacte ...
23 development of molecular markers to distinguish cytoplasm
23 development of molecular markers to distinguish cytoplasm

... fragment also yielded two groups, separating the D8 and B1 lines from the remaining alloplasms in the study. The identity of these lines was confirmed. The low level of polymorphisms found among the cytoplasms in relation to the number of primers used and digestions performed can be explained by the ...
Chp 8_6 - Western High School
Chp 8_6 - Western High School

... Substitutions usually affect no more than a single _______________________________, but deletions and insertions can have a more dramatic effect. ...
Bioinformatics - Oxford Academic
Bioinformatics - Oxford Academic

... data-mining backhoe is the fact that this book covers all the topics that, most of us would agree, make up bioinformatics in the twenty-®rst century. If your favourite method or program is not dealt with here, then a suitable, perhaps even better, equivalent will be. There is coverage of pre-genomic ...
Carrots and Genomics
Carrots and Genomics

... • Mimics the process of DNA duplication in the plant • The polymerase (enzyme) requires dsDNA to start building in nucleotides ...
Regulation of Gene Activity in Eukaryotes
Regulation of Gene Activity in Eukaryotes

... from the body in order to genetically to modify them, nor can all types of cell be grown successfully in culture. • There are major problems inherent in attempting to introduce foreign DNA into specific types of human cells in vivo. – viral vector infect only certain type of cells in vivo – lack sel ...
242413_Fx_DNA_Fingerprinting_Lab
242413_Fx_DNA_Fingerprinting_Lab

... 3. If you inherited a chromosome from your father that had 7 repeats at a certain locus (location of a specific gene) and a chromosome from your mother that had 12 repeats, what would your genotype be for this specific repeating sequence? Genetics – Today’s DNA Profile 1. How many STRs are analyzed ...
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Genome editing

Genome editing, or genome editing with engineered nucleases (GEEN) is a type of genetic engineering in which DNA is inserted, replaced, or removed from a genome using artificially engineered nucleases, or ""molecular scissors."" The nucleases create specific double-stranded break (DSBs) at desired locations in the genome, and harness the cell’s endogenous mechanisms to repair the induced break by natural processes of homologous recombination (HR) and nonhomologous end-joining (NHEJ). There are currently four families of engineered nucleases being used: Zinc finger nucleases (ZFNs), Transcription Activator-Like Effector Nucleases (TALENs), the CRISPR/Cas system, and engineered meganuclease re-engineered homing endonucleases.It is commonly practiced in genetic analysis that in order to understand the function of a gene or a protein function one interferes with it in a sequence-specific way and monitors its effects on the organism. However, in some organisms it is difficult or impossible to perform site-specific mutagenesis, and therefore more indirect methods have to be used, such as silencing the gene of interest by short RNA interference (siRNA) . Yet gene disruption by siRNA can be variable and incomplete. Genome editing with nucleases such as ZFN is different from siRNA in that the engineered nuclease is able to modify DNA-binding specificity and therefore can in principle cut any targeted position in the genome, and introduce modification of the endogenous sequences for genes that are impossible to specifically target by conventional RNAi. Furthermore, the specificity of ZFNs and TALENs are enhanced as two ZFNs are required in the recognition of their portion of the target and subsequently direct to the neighboring sequences.It was chosen by Nature Methods as the 2011 Method of the Year.
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