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Cellular ageing processes - Homepages | The University of Aberdeen
Cellular ageing processes - Homepages | The University of Aberdeen

... Cellular ageing in yeast Reference: Sinclair & Guarente (1997), Cell 91, 1033-1042. Ageing is a complex process in higher organisms, and it is not fully understood. However ageing also applies to microorganisms such as yeast, and these simple systems can be used to investigate certain aspects of th ...
Lecture 11 Analysis of Gene Sequences Anatomy of a bacterial
Lecture 11 Analysis of Gene Sequences Anatomy of a bacterial

... recognized because of mutations in the gene that give an observable phenotypic change. Historically, many genes have been discovered because of their effects on phenotype. Now, in the era of genomic sequencing, many genes of no known function can be detected by looking for patterns in DNA sequences. ...
Transposons - iPlant Pods
Transposons - iPlant Pods

... • Subtle impact on the expression of many genes • Produces stress-inducible networks (cold, salt, others?) • Generates dominant alleles Naito et al, Nature, 2009 ...
Is it on or off? The Use of Microarrays in Functional Genomics
Is it on or off? The Use of Microarrays in Functional Genomics

... One of the most important applications being employed with microarrays is with a phenomenon called expression profiling, which measures the amount of gene expression by assessing the levels of mRNA abundance. For the most part, the assumption is made that changes in mRNA amounts correspond to change ...
Activity 1: How Mendel`s Pea Plants Helped Us With Genetics You
Activity 1: How Mendel`s Pea Plants Helped Us With Genetics You

... Genetics is the science of genes and heredity. Inside the nucleus of all your body cells are 23 pairs of chromosomes. Each chromosome carries hundreds to thousands of genes on it. Each gene describes some of your traits. Traits include characteristics like your hair color, eye color, allergies, body ...
Bio 101 Homework #3 Prof. Fournier
Bio 101 Homework #3 Prof. Fournier

... flounder DNA that is then spliced into the DNA of a strawberry plant. As a result, the plant can now produce a protein that makes it more resistant to the damaging effects of frost. What is this process known as? A) B) C) D) ...
Genetic Changes - Down the Rabbit Hole
Genetic Changes - Down the Rabbit Hole

... • Cystic Fibrosis • Down Syndrome • Some are beneficial • Sickle Cell Anemia to Malaria • Immunity to HIV ...
Document
Document

... traits, they both appear). There is another blood type that is somewhat similar, the MN blood typing system. It is not as medically important, so it is not discussed as often. For the gene in question, the M and N forms each code for different types of glycoproteins (proteins with sugars attached to ...
Document
Document

... If recombination rate is high, genes are far apart. ...
Eukaryotic Gene Expression Practice Problems Class Work 1
Eukaryotic Gene Expression Practice Problems Class Work 1

... b. All cells in a eukaryotic organism contain a complete genome. Selective gene expression allows cells to selectively express genes that will produce the types of proteins the cell needs to perform specific functions. Muscle cells and brain cells, for example, have very different structures and per ...
Genetics of bacteria and bacteriophages
Genetics of bacteria and bacteriophages

... If recombination rate is high, genes are far apart. ...
Eukaryotic Gene Expression Practice Problems Class Work 1
Eukaryotic Gene Expression Practice Problems Class Work 1

... b. All cells in a eukaryotic organism contain a complete genome. Selective gene expression allows cells to selectively express genes that will produce the types of proteins the cell needs to perform specific functions. Muscle cells and brain cells, for example, have very different structures and per ...
Lesson 12 Mutations
Lesson 12 Mutations

...  A small-scale change in the nitrogenous base sequence of DNA. A point mutation is a failure by the replicating cell to copy the genetic information accurately. Point mutations may be beneficial, harmful, or neutral (having no effect on the organism). There are three major point mutations. ...
Name
Name

... 15. Malaria is a disease caused by parasites that reproduce within red blood cells. Malaria can cause fever, chills, nausea, flu-like symptoms, and in some cases, coma and death. This disease is prevalent in warm climates, especially South America, Africa, and the southern portions of Asia. Sickle- ...
Plant Functional Genomics
Plant Functional Genomics

hox genes
hox genes

... vitripennis [6], Hox genes are organized in a contiguous, single-copy cluster in the leafcutter ant Atta cephalotes. However, only 9 of the 10 genes usually found in the cluster were identified here (Figure1). Despite sequence similarity analyses against the entire genome, a homolog for Hox3-A was n ...
Genomics
Genomics

... and related techniques.  The main difference between genomics and genetics is that genetics scrutinizes the functioning and composition of the single gene where as genomics addresses all genes and their inter relationships in order to identify their combined influence on the growth and development ...
Apple Molecular Biology: Animation 1
Apple Molecular Biology: Animation 1

... contributes the overall general knowledge as well as allowing researchers to make comparative studies between the genomes of crop species and other known model plant species. Conclusions drawn from such comparisons help scientists understand the relationships between gene composition and function at ...
Gene Therapy
Gene Therapy

RUNX1-RUNX1T1 pre
RUNX1-RUNX1T1 pre

... SPLICING IS ASSOCIATED WITH SEQUENCE-RELATED FEATURES ...


... * DNA copy gain/loss within a known clinically significant gene region of 50 Kb or greater. * DNA copy number loss of >200 kb or gain >500 kb outside known clinically significant regions with at least one OMIM annotated gene or within a region of clear clinical significance. * UPD testing is recomme ...
Chromosome Contact Matrices
Chromosome Contact Matrices

... The dynamic algorithm will find the global optimum, provided that the data is consistent with the compartment model For every compartment run the method recursively, on re-normalized sub-matrix The process naturally stops when all vectors in the compartment have positive correlation Works in polynom ...
Structural and Functional Genomics of Tomato
Structural and Functional Genomics of Tomato

...  2) Develop deep EST databases from various Solanaceae tissues and shotgun genomic sequencing of other Solanaceae with data integration.  4) Construct a set of interspecific introgression resources (e.g. introgression lines, backcross inbred lines etc.) for all Solanaceae crop species in order to ...
STRs and Marker Analysis
STRs and Marker Analysis

Ensembl. Going beyond A,T, G and C
Ensembl. Going beyond A,T, G and C

... GenCode 5’ and CAGE/DiTag ...
< 1 ... 265 266 267 268 269 270 271 272 273 ... 445 >

Genome editing

Genome editing, or genome editing with engineered nucleases (GEEN) is a type of genetic engineering in which DNA is inserted, replaced, or removed from a genome using artificially engineered nucleases, or ""molecular scissors."" The nucleases create specific double-stranded break (DSBs) at desired locations in the genome, and harness the cell’s endogenous mechanisms to repair the induced break by natural processes of homologous recombination (HR) and nonhomologous end-joining (NHEJ). There are currently four families of engineered nucleases being used: Zinc finger nucleases (ZFNs), Transcription Activator-Like Effector Nucleases (TALENs), the CRISPR/Cas system, and engineered meganuclease re-engineered homing endonucleases.It is commonly practiced in genetic analysis that in order to understand the function of a gene or a protein function one interferes with it in a sequence-specific way and monitors its effects on the organism. However, in some organisms it is difficult or impossible to perform site-specific mutagenesis, and therefore more indirect methods have to be used, such as silencing the gene of interest by short RNA interference (siRNA) . Yet gene disruption by siRNA can be variable and incomplete. Genome editing with nucleases such as ZFN is different from siRNA in that the engineered nuclease is able to modify DNA-binding specificity and therefore can in principle cut any targeted position in the genome, and introduce modification of the endogenous sequences for genes that are impossible to specifically target by conventional RNAi. Furthermore, the specificity of ZFNs and TALENs are enhanced as two ZFNs are required in the recognition of their portion of the target and subsequently direct to the neighboring sequences.It was chosen by Nature Methods as the 2011 Method of the Year.
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