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Ch_20
Ch_20

... lacZ gene and many cuts within the human DNA. 3 Mix the DNAs; they join by base pairing. The products are recombinant plasmids and many nonrecombinant plasmids. ...
Feb. 11-12 Day 2: The Work of Gregor Mendel
Feb. 11-12 Day 2: The Work of Gregor Mendel

... LAB: The Law of Probability Should this Dog be Called Spot Feb 19-20 Day 4: Exploring Mendel’s Genetics OBJECTIVES: 1. Describe how two-factor crosses illustrate the principle of independent assortment. 2. Describe the inheritance patterns that exist aside from simple dominance. 3. Explain how Mende ...
Snork Activity
Snork Activity

... sequence of amino acids in proteins. The sequence of DNA is the most important part of determining what proteins are synthesized.  During transcription, which takes place in the nucleus of the cell, messenger RNA (mRNA) molecules are built along the DNA sequence into a single RNA strand. mRNA leave ...
Answer Key
Answer Key

... industrial melanism ...
Introduction to DNA Microarrays
Introduction to DNA Microarrays

... which code for the protein into RNA used in its production – The RNA present in a cell can be extracted – If a gene has been expressed in a cell ...
Document
Document

... analysis we measured: accuracy, specificity and sensitivity. Accuracy was defined as (TP + TN)/(TP + FP + FN + TN), specificity as TN/(TN + FP) and sensitivity as TP/(TP + FN) where TP is the number of true positives, TN is the number of true negatives, FP is the number of false positives and FN is ...
General
General

...  KEGG does not predict presence or absence of pathways  KEGG lacks pathway hole filler, operon predictor  Curation tools  KEGG does not distribute curation tools  No ability to customize pathways to the organism  Pathway Tools schema much more comprehensive  Visualization and analysis  KEGG ...
Investigation 1: Identify the Transcriptional Unit
Investigation 1: Identify the Transcriptional Unit

... proteins are required for transcription? How does it work mechanistically? What is/are the products of transcription? (students discuss in pairs, then as a class) Work through the genome browser investigation, then identify where transcription starts and ends for the tra gene. How long is the pre-mR ...
Mutations Handout
Mutations Handout

... ______18. Why are insertion and deletion mutations usually more serious than substitutions? A. they can be passed on to offspring B. they change every codon after the mutation C. they always cause some form of cancer D. they cause recessive traits to become dominant traits ______19. Why do some gen ...
Recombination in Bacteria Overview This module looks at how the
Recombination in Bacteria Overview This module looks at how the

... amount of the mixture would be removed and conjugation would be disrupted using a blender (the shear force of the blender would cause any pili to break). These bacteria would then be tested for gene conversion (for example, if the mutations rendered the F- bacteria auxotrophic, the bacteria could be ...
Identification of an Insertion Sequence Located
Identification of an Insertion Sequence Located

... promote microbial evolution and can be facilitated by insertion sequences (IS). These mobile genetic elements, by definition, contain genes related only to insertion functions (4). Despite this definition, the phenotype of the recipient bacterium can be changed if the IS is inserted into a structura ...
Identification of porcine Lhx3 and SF1 as candidate genes for QTL
Identification of porcine Lhx3 and SF1 as candidate genes for QTL

BrownCNA Thank you with the QC checking of this genome. It was
BrownCNA Thank you with the QC checking of this genome. It was

The study of threshold determination of gene identification and its
The study of threshold determination of gene identification and its

... information biology. Firstly, this essay will discuss the threshold determination of different species types of genes. To determine the threshold of genes types in different species, and to study the threshold determination method of each kind of representative gene sequence exons, and determine the ...
printer-friendly version
printer-friendly version

... most of DNA is quite similar. Based on sequencing to date it appears that on average two unrelated people have one different nucleotide per 1000 bases. Thus with 3 billion bp total bases this means there are 3 million differences between individuals or less than 0.01% difference between individuals. ...
Gill: Transcription Regulation I
Gill: Transcription Regulation I

Introduction to Biological Data
Introduction to Biological Data

... 1 The LOCUS field ...
Recombinant DNA Technology
Recombinant DNA Technology

... 3. A plasmid is a circular, double stranded piece of DNA that occurs naturally in bacteria and can be used as an important tool in genetic engineering. A human gene can be inserted into a plasmid (this is used as a vector to transfer the gene into a bacterial cell), and then this DNA is absorbed by ...
pptx - Fenyo Lab
pptx - Fenyo Lab

... Protein Sequence Databases • Identification of peptides from MS relies heavily on the quality of the protein sequence ...
PPT - Blumberg Lab
PPT - Blumberg Lab

Gen660_Lecture3A_Ortho
Gen660_Lecture3A_Ortho

... * Clear cases where the top BLAST hit is NOT the ortholog e.g. top hits can be highly conserved common domains ...
PAG 2012 - Illumina
PAG 2012 - Illumina

... to see a live demo of the MiSeq® personal sequencer. Learn more at www.illumina.com/MiSeq ...
Lecture 1 – Mendelian inheritance
Lecture 1 – Mendelian inheritance

... outcomes in a number of trials that could each have either of two possible outcomes e.g., determining the probability of 1 female and 4 male children in a family with 5 children ...
Case Study: Visualization of annotated DNA sequences
Case Study: Visualization of annotated DNA sequences

... the minimum of all start positions and max the maximum of all end positions of the regions of α. properties is a set of name-value pairs representing properties that are not contained in the standard attributes. Annotations are supplied as files in GFF format [GFF]. There are many types of annotatio ...
Biol 101 Study Guide Exam 5
Biol 101 Study Guide Exam 5

... hair, etc., from your body behind because if you do, the DNA in this material can be amplified by //___, subjected to genetic analysis, and used to identify you as the perpetrator of the crime. 15) ______ A) ATP B) blotting C) PCR D) RFLP E) reverse transcriptase 16) The polymerase chain reaction re ...
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Genome editing

Genome editing, or genome editing with engineered nucleases (GEEN) is a type of genetic engineering in which DNA is inserted, replaced, or removed from a genome using artificially engineered nucleases, or ""molecular scissors."" The nucleases create specific double-stranded break (DSBs) at desired locations in the genome, and harness the cell’s endogenous mechanisms to repair the induced break by natural processes of homologous recombination (HR) and nonhomologous end-joining (NHEJ). There are currently four families of engineered nucleases being used: Zinc finger nucleases (ZFNs), Transcription Activator-Like Effector Nucleases (TALENs), the CRISPR/Cas system, and engineered meganuclease re-engineered homing endonucleases.It is commonly practiced in genetic analysis that in order to understand the function of a gene or a protein function one interferes with it in a sequence-specific way and monitors its effects on the organism. However, in some organisms it is difficult or impossible to perform site-specific mutagenesis, and therefore more indirect methods have to be used, such as silencing the gene of interest by short RNA interference (siRNA) . Yet gene disruption by siRNA can be variable and incomplete. Genome editing with nucleases such as ZFN is different from siRNA in that the engineered nuclease is able to modify DNA-binding specificity and therefore can in principle cut any targeted position in the genome, and introduce modification of the endogenous sequences for genes that are impossible to specifically target by conventional RNAi. Furthermore, the specificity of ZFNs and TALENs are enhanced as two ZFNs are required in the recognition of their portion of the target and subsequently direct to the neighboring sequences.It was chosen by Nature Methods as the 2011 Method of the Year.
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