Kinetic Role of Helix Caps in Protein Folding Is Context
... described and classified (8). In this study, the N- and C-cap residues are defined as the first and last residues of the helix with CR atoms within the helical spiral (7) (see Materials and Methods for exact details). Extensive analyses by several groups have shown that helix caps at the N-terminus ...
... described and classified (8). In this study, the N- and C-cap residues are defined as the first and last residues of the helix with CR atoms within the helical spiral (7) (see Materials and Methods for exact details). Extensive analyses by several groups have shown that helix caps at the N-terminus ...
Cloning and sequencing of a gene encoding acidophilic amylase
... Agnano 101 (Buonocore et at., 1976) and A-2 (Kanno, 1986), and from Bacillus sp. 11-1s (Uchino, 1982). The pH optima of the three enzymes ranged from 2-0 to 3.5, temperature optima between 70 and 75 "C and apparent molecular masses from 54 to 68 kDa. The molecular mass of the B. acidocaldarius ATCC ...
... Agnano 101 (Buonocore et at., 1976) and A-2 (Kanno, 1986), and from Bacillus sp. 11-1s (Uchino, 1982). The pH optima of the three enzymes ranged from 2-0 to 3.5, temperature optima between 70 and 75 "C and apparent molecular masses from 54 to 68 kDa. The molecular mass of the B. acidocaldarius ATCC ...
Current Microbiology
... Isolation and Characterization of Transposon-Insertional Mutants from Paenibacillus polymyxa E681 Altering the Biosynthesis of ...
... Isolation and Characterization of Transposon-Insertional Mutants from Paenibacillus polymyxa E681 Altering the Biosynthesis of ...
MORINGA OLEIFERA IN SILICO Research Article
... palmitate and Ethyl stearate. The scores of Ethyl stearate and Ethyl Oleate with all the target proteins except nGCNT1 indicated that the complementarity decreased with unsaturation. In general, the scores increased with increase in the molecular weight of the ligands and might not be proportional t ...
... palmitate and Ethyl stearate. The scores of Ethyl stearate and Ethyl Oleate with all the target proteins except nGCNT1 indicated that the complementarity decreased with unsaturation. In general, the scores increased with increase in the molecular weight of the ligands and might not be proportional t ...
Metal ion reconstitution studies of yeast copper
... was measured using an Orion Research Microprocessor Ionalyzer/901 (Boston, Mass.) with an Ingold microelectrode (Wilmington, Mass.). Native gel electrophoresis using 9% acrylamide was performed in accordance with published procedures at 4 7C [26]. ...
... was measured using an Orion Research Microprocessor Ionalyzer/901 (Boston, Mass.) with an Ingold microelectrode (Wilmington, Mass.). Native gel electrophoresis using 9% acrylamide was performed in accordance with published procedures at 4 7C [26]. ...
The use of deuteration for the structural study of larger proteins
... β transitions) this leads to nuclear relaxation. • The required fluctuations of the local magnetic fields can be caused by e.g. brownian motion (overall rotational tumbling of a molecule) or due to e.g. internal mobility within a molecule. ...
... β transitions) this leads to nuclear relaxation. • The required fluctuations of the local magnetic fields can be caused by e.g. brownian motion (overall rotational tumbling of a molecule) or due to e.g. internal mobility within a molecule. ...
The size, operation, and technical capabilities of protein and nucleic
... and off-line PTH amino acid HPLC systems but not HPLC systems dedicated solely to the analysis of free amino acids or their phenylthiocarbamyl derivatives; these are included under amino acid analyzers. ...
... and off-line PTH amino acid HPLC systems but not HPLC systems dedicated solely to the analysis of free amino acids or their phenylthiocarbamyl derivatives; these are included under amino acid analyzers. ...
Variations in amino acid composition in bacterial single stranded
... Thus, SSBs prevent degradation of ssDNA and the formation of unproductive secondary structures (2). One of the most extensively studied bacterial SSB belongs to Escherichia coli (EcoSSB) (3, 4). Apart from passive protection of cellular ssDNA, SSB also has a second less known ...
... Thus, SSBs prevent degradation of ssDNA and the formation of unproductive secondary structures (2). One of the most extensively studied bacterial SSB belongs to Escherichia coli (EcoSSB) (3, 4). Apart from passive protection of cellular ssDNA, SSB also has a second less known ...
Inositol 1,3,4,5,6-Pentakisphosphate 2-Kinase
... 2003). Radioisotope labeling experiments with duckweed (Spirodela polyrhiza), an aquatic plant, suggest that a lipid-independent pathway may be operational in plants. The intermediates of this phytic acid biosynthetic route were reported to be Ins(3)P1, Ins(3,4)P2, Ins(3,4,6)P3, Ins(3,4,5,6)P4, and ...
... 2003). Radioisotope labeling experiments with duckweed (Spirodela polyrhiza), an aquatic plant, suggest that a lipid-independent pathway may be operational in plants. The intermediates of this phytic acid biosynthetic route were reported to be Ins(3)P1, Ins(3,4)P2, Ins(3,4,6)P3, Ins(3,4,5,6)P4, and ...
Isolation and Characterization of Two Polypeptides
... cytoplasmic component of cells in stratified squamous epithelia . They are often referred to as tonofilaments and are morphologically similar in different epithelia: they are 6-10 nm in diameter, they form undulating, unbranched curves in the cytoplasm, their length is often several hundred times th ...
... cytoplasmic component of cells in stratified squamous epithelia . They are often referred to as tonofilaments and are morphologically similar in different epithelia: they are 6-10 nm in diameter, they form undulating, unbranched curves in the cytoplasm, their length is often several hundred times th ...
Bioinformatics
... 3. DNA DataBank of Japan (DDBJ) at Mishima, Japan http://www.ddbj.nig.ac.jp/ 4. Protein International Resource (PIR) database at the National Biomedical Research Foundation in Washington, DC (see Barker et al. 1998) http://wwwnbrf.georgetown.edu/pirwww/ 5. The SwissProt protein sequence database at ...
... 3. DNA DataBank of Japan (DDBJ) at Mishima, Japan http://www.ddbj.nig.ac.jp/ 4. Protein International Resource (PIR) database at the National Biomedical Research Foundation in Washington, DC (see Barker et al. 1998) http://wwwnbrf.georgetown.edu/pirwww/ 5. The SwissProt protein sequence database at ...
Peroxisomes: family of versatile organelles
... • diameter: peroxisomes 0.5µm; microperoxisomes 0.1-0.2µm • single membrane • no own DNA ...
... • diameter: peroxisomes 0.5µm; microperoxisomes 0.1-0.2µm • single membrane • no own DNA ...
Ecological and molecular investigations of cyanotoxin production
... ters on toxin production. Hypotheses regarding the ecophysiology of the cyanotoxins, stemming from such studies, are discussed in this review. With advances in molecular biology, it has more recently been possible to elucidate the genetics behind the biosynthetic pathways of some of these toxins [3] ...
... ters on toxin production. Hypotheses regarding the ecophysiology of the cyanotoxins, stemming from such studies, are discussed in this review. With advances in molecular biology, it has more recently been possible to elucidate the genetics behind the biosynthetic pathways of some of these toxins [3] ...
Sildenafil (Viagra) – A Patient Case Related to Medicinal Chemistry
... The body’s main pathway for signaling vasodilation is the NO-sGC-cGMP pathway shown in Figure 1. In this pathway: NO signals for an increase in intracellular cGMP which causes vasodilation. Phosphodiesterase enzymes break down cGMP causing vasoconstriction. Drugs target this pathway via several mech ...
... The body’s main pathway for signaling vasodilation is the NO-sGC-cGMP pathway shown in Figure 1. In this pathway: NO signals for an increase in intracellular cGMP which causes vasodilation. Phosphodiesterase enzymes break down cGMP causing vasoconstriction. Drugs target this pathway via several mech ...
The maize ID1 flowering time regulator is a zinc finger protein with
... a-helix of each ®nger interact with a triplet of base pairs (5,6). In plants, the C2H2 class of zinc ®nger proteins is one of the largest families of transcription factors to be identi®ed, although they appear to not be as prevalent as C2H2 proteins in animals (7,8). Genetic and functional analyses ...
... a-helix of each ®nger interact with a triplet of base pairs (5,6). In plants, the C2H2 class of zinc ®nger proteins is one of the largest families of transcription factors to be identi®ed, although they appear to not be as prevalent as C2H2 proteins in animals (7,8). Genetic and functional analyses ...
Passenger Protein Determines Translocation Versus Retention in
... of these regions (Szczesna-Skorupa et al., 1988; Kuroiwa et al., 1991). Thus, the exact mechanism by which newly synthesized proteins translocate across the ER membrane remains unclear. It is still unknown why some proteins completely translocate across the ER bilayer whereas others stay partially e ...
... of these regions (Szczesna-Skorupa et al., 1988; Kuroiwa et al., 1991). Thus, the exact mechanism by which newly synthesized proteins translocate across the ER membrane remains unclear. It is still unknown why some proteins completely translocate across the ER bilayer whereas others stay partially e ...
prosthetic group as non polypeptide biocatalyst essential for
... these enzymes in allosteric regulation, often binding to these enzymes in a complex with calmodulin.[18] Calcium is, therefore, a cell signaling molecule and not usually considered a cofactor of the enzymes it regulates. Other ...
... these enzymes in allosteric regulation, often binding to these enzymes in a complex with calmodulin.[18] Calcium is, therefore, a cell signaling molecule and not usually considered a cofactor of the enzymes it regulates. Other ...
Solution Blowing of Soy Protein Fibers
... control, soy protein isolate was also subjected to the same treatment and it was found that it was never fully destroyed and left residual quite comparable in volume to the original soy protein. Therefore, it was established that after such treatment the PAN-based core−shell samples will not be holl ...
... control, soy protein isolate was also subjected to the same treatment and it was found that it was never fully destroyed and left residual quite comparable in volume to the original soy protein. Therefore, it was established that after such treatment the PAN-based core−shell samples will not be holl ...
Effects of rare codon clusters on high-level expression
... heterologous product in an E. coli K-12 host containing a chromosomal copy o f ~. ci857 gene. A temperature shiti upwards induced product expression. Growth and expression occurred in 10 liter fermenters. Western analysis indicated a full-length protein together with one major and two minor molecula ...
... heterologous product in an E. coli K-12 host containing a chromosomal copy o f ~. ci857 gene. A temperature shiti upwards induced product expression. Growth and expression occurred in 10 liter fermenters. Western analysis indicated a full-length protein together with one major and two minor molecula ...
NAD - SBI
... Uchikoba H, Fushinobu S, Wakagi T, Konno M, Taguchi H, Matsuzawa H .Crystal Structure of Non-Allosteric L-Lactate Dehydrogenase From Lactobacillus pentosus at 2.3 Å Resolution: Specific Interactions at Subunit Interfaces. PROTEINS: Structure, Function, and Genetics. 2002;46:206–214. Michel G, Roszak ...
... Uchikoba H, Fushinobu S, Wakagi T, Konno M, Taguchi H, Matsuzawa H .Crystal Structure of Non-Allosteric L-Lactate Dehydrogenase From Lactobacillus pentosus at 2.3 Å Resolution: Specific Interactions at Subunit Interfaces. PROTEINS: Structure, Function, and Genetics. 2002;46:206–214. Michel G, Roszak ...
Adaptations of protein structure and function to temperature: there is
... the enzyme. Consequently, as temperature increases, enzyme reaction rates first tend to accelerate, as stabilizing bonds break and reform more rapidly, and the conformational changes in the protein necessary for catalysis occur at a faster pace. However, as temperature continues to rise, enough stab ...
... the enzyme. Consequently, as temperature increases, enzyme reaction rates first tend to accelerate, as stabilizing bonds break and reform more rapidly, and the conformational changes in the protein necessary for catalysis occur at a faster pace. However, as temperature continues to rise, enough stab ...
Feed Ingredients and Feeds for Channel Catfish
... obtained by grinding the flakes remaining after removing most of the oil from dehulled soybeans by a solventextraction process. It contains about 48 percent highquality protein and is the predominant protein source in catfish feeds. Soybean meal has the best amino acid profile of all common plant pr ...
... obtained by grinding the flakes remaining after removing most of the oil from dehulled soybeans by a solventextraction process. It contains about 48 percent highquality protein and is the predominant protein source in catfish feeds. Soybean meal has the best amino acid profile of all common plant pr ...
IndelFR: a database of indels in protein structures and their flanking
... evolutionary history (9,10). However, there are few studies of the sequence–structure–function relationships of indels. Indels usually occur as reverse turns or coils within loops in a domain, most often on the surface of proteins (11–13). Compared to deletions, a succession of insertions and rapid ...
... evolutionary history (9,10). However, there are few studies of the sequence–structure–function relationships of indels. Indels usually occur as reverse turns or coils within loops in a domain, most often on the surface of proteins (11–13). Compared to deletions, a succession of insertions and rapid ...
Food derived from glufosinate ammonium
... Tu494. It codes for the enzyme phosphinothricin acetyl transferase (PAT), which modifies and inactivates the herbicide glufosinate ammonium (Strauch et al, 1988). The pat gene is often used as a selectable marker to distinguish genetically modified plant cells from unmodified cells. In this applicat ...
... Tu494. It codes for the enzyme phosphinothricin acetyl transferase (PAT), which modifies and inactivates the herbicide glufosinate ammonium (Strauch et al, 1988). The pat gene is often used as a selectable marker to distinguish genetically modified plant cells from unmodified cells. In this applicat ...
Interactome
In molecular biology, an interactome is the whole set of molecular interactions in a particular cell. The term specifically refers to physical interactions among molecules (such as those among proteins, also known as protein-protein interactions) but can also describe sets of indirect interactions among genes (genetic interactions). Mathematically, interactomes are generally displayed as graphs.The word ""interactome"" was originally coined in 1999 by a group of French scientists headed by Bernard Jacq. Though interactomes may be described as biological networks, they should not be confused with other networks such as neural networks or food webs.