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CHEMISTRY OF AQUATIC ORGANISMS AND THEIR UTILIZATION
CHEMISTRY OF AQUATIC ORGANISMS AND THEIR UTILIZATION

... Fig. 4.4. Molecular dendrogram of globin superfamily based on amino acid sequences. Each protein forms clear clusters. ...
Comparative Analysis of Prothrombin Activators
Comparative Analysis of Prothrombin Activators

... for the factor X–like protease precursor, identified in elapids containing either group C or D prothrombin activators, demonstrated a remarkable degree of relatedness to each other (80%–97%). The factor V–like component of the prothrombin activator, present only in snakes containing group C complexe ...
Purified Hexameric Epstein-Barr Virus-Encoded BARF1 Protein for Measuring Anti-BARF1 Antibody
Purified Hexameric Epstein-Barr Virus-Encoded BARF1 Protein for Measuring Anti-BARF1 Antibody

... Sera from NPC patients and healthy EBV carriers. Serum panels from histologically confirmed NPC patients (n ⫽ 155) were collected at the Department of Ear, Nose, and Throat (ENT), Dr. Sardjito General Hospital, Yogyakarta, Indonesia. NPC sera were taken prior to treatment. NPC staging was done by EN ...
structure-helix-text
structure-helix-text

... Pro: least common residue in α-helix because of its rigid cyclic side chain disrupts the helical structure by occupying space that a neighboring residue of the helix would otherwise occupy CHMI 2227 - E.R. Gauthier, Ph.D. ...
The RNA-binding protein HuR regulates protein nuclear import
The RNA-binding protein HuR regulates protein nuclear import

Article Fitness Trade-Offs Determine the Role of the Molecular
Article Fitness Trade-Offs Determine the Role of the Molecular

Free amino acid content in infant formulas
Free amino acid content in infant formulas

... formulas (Giovannini et al., 1994; Hernell and Lonnerdal, 2003; Rigo et al., 1995), the consequence of which is still unclear. The effects, if any, that the relative and absolute levels of FAAs in breast milk or formulas have on the establishment of long-term flavor and food preferences and understan ...
Amino Acid Incorporation by in Vitro Tumor and
Amino Acid Incorporation by in Vitro Tumor and

... The addition of polyuridylic acid to the tumor system markedly stimulated the incorporation of phenylalanine-C'4, and as little as 50 @ig.of the synthetic polynucleotide resulted in a seven-fold average increase in the radioactivity (Table 3). In some instances, the incorporation was raised from 27 ...


... a matrix, but unlike global matrix, it has an extra row along the top and an extra column on the left side which are filled in with zeros. Therefore, for two sequences of length "m" and "n", the matrix dimension will be "m+1" and "n+1". In the first stage the match and mismatch letters are scored. M ...
Prediction of B cell epitopes
Prediction of B cell epitopes

... antigen complexes in the PDB  The data set has been used for developing a method for predictions of discontinuous B cell epitopes  Since about 30 of the PDB entries represented Lysozyme, I have used homology grouping (25 groups of non-homologous antigens) and 5 fold cross-validation for training o ...
Constant Growth Rate Can Be Supported by Decreasing Energy
Constant Growth Rate Can Be Supported by Decreasing Energy

... continuously changing metabolic fluxes per cell (Figures 2E and 2F) during exponential growth suggest that the cells may not be at steady state even when their growth rate remains constant; this observation is so unexpected that we sought to test it further by multiple independent experimental measu ...
The sequence of human serum albumin cDNA and its expression in
The sequence of human serum albumin cDNA and its expression in

... An additional cDNA clone extending even farther 5' was obtained by similar oligodeoxynucleotide primed cDNA synthesis (from a primer corresponding to amino acid codons no. 175-179). Although not employed in the construction of the mature HSA expression plasmid, this cDNA clone (P-14) allowed determi ...
REGULATORY MECHANISMS OF CELLULAR RESPIRATION I
REGULATORY MECHANISMS OF CELLULAR RESPIRATION I

... so (1) by combining with the activating protein, either through some groups essential for activity (for example, the - S H groups), through denaturation of the molecule, or by combination on the side chains where substrates or prosthetic groups form the protein-substrate complex (structural inhibito ...
Structure and mechanism of action of a novel
Structure and mechanism of action of a novel

... (Chander et al., 1998). These latter organisms include members of Staphylococcus and Streptococcus species, some of which are important human pathogens. Consequently, it is possible that iPGMs could be a target for rational design of a novel antibiotic. Interestingly, some bacteria have genes for bo ...
Selected Reaction Monitoring (SRM) to determine protein
Selected Reaction Monitoring (SRM) to determine protein

... confirming the reduction in abundance of the encoded protein will often aid the link between genotype and phenotype. However, measuring specific protein abundance is particularly difficult in plant research where only a limited number of antibodies are available. This problem is enhanced when studyi ...
Effects of signaling on subcellular localization of MITF
Effects of signaling on subcellular localization of MITF

... regulated by signaling, for example through the MAPK pathway which mediates signals with protein phosphorylations. The MAPK pathway is deregulated in at least 90% of all melanomas. When transcription factors are phosphorylated, it can affect their activity, stability, protein interactions and their ...
Chapter 1 - Nutrition Gardener
Chapter 1 - Nutrition Gardener

... activity can make for anxiety and depression as well as weight and weight loss. Physical activity for such things as stress can dissipate stress hormones. This can be so helpful for the obese individual that learned to eat in response to stress. If an individual’s self-esteem has been lowered becau ...
Isolation of casein from milk
Isolation of casein from milk

... • Is a phosphoprotein, which has phosphate groups attached to some of the amino acid side chains. Mostly these amino acid are serine and threonine. • casein is a mixture of at least three similar proteins, which differ primarily in molecular weight and amount of phosphorus they contain (number of ph ...
Sequence ID: ref|WP_006700522.1
Sequence ID: ref|WP_006700522.1

... Clone several, using one known to work to find orthologs Use sequence to design primers to clone cDNA ...
FRET!
FRET!

... Responses: Fluid secretion, exocytosis, channel gating, enzyme activities, cell division, proliferation, gene expression ...
What is Xtend - Bodybuilding.com Forums
What is Xtend - Bodybuilding.com Forums

... protein synthesis is met leucine can then be used as a precursor for alanine and glutamine or to activate various signaling pathways (Layman, 2003), such as the mTOR pathway. It may sound like leucine is free to exert its powerful effect of mTOR activation, but one must remember that protein breakdo ...
Molecular Characterization of a Hamster Oviduct
Molecular Characterization of a Hamster Oviduct

... of the mature form of the HOGP region. The amino acid sequence of HOGP appeared to have eight potential N-glycosylation sites. Northern blot analysis revealed that a single message of approximately 2.5 kb was present inoviductal RNA but not inthe RNA of several other hamster tissues. The HOGP showed ...
Akashi_Gojobori.PNAS02
Akashi_Gojobori.PNAS02

12 Molecular Visualization of an Enzyme, Acetylcholinesterase
12 Molecular Visualization of an Enzyme, Acetylcholinesterase

... and myasthenia gravis target acetylcholinesterase. Examples of these drugs include physostigmine (eserine), neostigmine, and pyridostigmine. How is the activity of acetylcholinesterase impacted by these insecticides or drugs? How does this change in acetylcholinesterase affect nerve transmission? ...
PDF - Biotechnology for Biofuels
PDF - Biotechnology for Biofuels

... and N2 atmosphere. The culture was monitored during the 25 h of fermentation by which time the pH had dropped naturally to about 4.5 (Figure 1A). Directly after the lag phase there was a rapid consumption of glucose and an increase in growth. In total 17.16 ± 0.84 L of biogas were produced. Given th ...
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Two-hybrid screening



Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.
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